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| 1 | HBV vaccine efficacy and detection and genotyping of vaccineé asymptomatic breakthrough HBV infection in Egypt显示文摘AIM:To evaluate the impact of mass vaccination against the hepatitis B virus (HBV) in Egypt,and to search for vaccinee asymptomatic breakthrough HBV infection and its genotype.METHODS:Seven hundred serum samples from vaccinated children and adults (aged 2-47 years) were used for quantitative and qualitative detection of HBsAb by ELISA.Three hundred and sixty serum samples representing undetectable or low or high HBsAb were screened for markers of active HBV infection (HBsAg,HBcAb (IgG) and HBeAb by ELISA,plus HBsAg by AxSYM) and HBV-DNA genotyping by nested multiplex PCR and by DNA sequencing.RESULTS:It was found that 65% of children aged 2-4 years,and 20.5% aged 4-13 years,as well as 45% adults were good responders to HBV vaccination mounting protective level HBsAb.Poor responders were 28%,59.5% and 34%,and non-responders were 7%,20% and 21% respectively,in the three studied groups.Markers of asymptomatic HBV infections were HBsAg detected by ELISA in 2.5% vs 11.39% by AxSYM.Other markers were HBcAb (IgG) in 1.38%,HBeAb in 0.83%,and HBV-DNA in 7.8%.All had HBV genotype E infection.CONCLUSION:It is concluded that HBV vaccine is efficient in controlling HBV infection among children and adults.The vaccine breakthrough infection was by HBV genotype E.A booster dose of vaccine is recommended,probably four years after initial vaccination. | Eman AE Abushady Magda MA Gameel John D Klena Salwa F Ahmed Kouka SE Abdel-wahab Sanya M Fahmy | 2011 | World Journal of Hepatology2011，3，6： | 5 |
| 2 | Development and optimization of thermal contrast amplification lateral flow immunoassays for ultrasensitive HIV p24 protein detection显示文摘Detection of human immunodeficiency virus(HIV)p24 protein at a single pg/ml concentration in point-of-care(POC)settings is important because it can facilitate acute HIV infection diagnosis with a detection sensitivity approaching that of laboratory-based assays.However,the limit of detection(LOD)of lateral flow immunoassays(LFAs),the most prominent POC diagnostic platform,falls short of that of laboratory protein detection methods such as enzyme-linked immunosorbent assay(ELISA).Here,we report the development and optimization of a thermal contrast amplification(TCA)LFA that will allow ultrasensitive detection of 8 pg/ml p24 protein spiked into human serum at POC,approaching the LOD of a laboratory test.To achieve this aim,we pursued several innovations as follows:(a)defining a new quantitative figure of merit for LFA design based on the specific to nonspecific binding ratio(BR);(b)using different sizes and shapes of gold nanoparticles(GNPs)in the systematic optimization of TCA LFA designs;and(c)exploring new laser wavelengths and power regimes for TCA LFA designs.First,we optimized the blocking buffer for the membrane and running buffer by quantitatively measuring the BR using a TCA reader.The TCA reader interprets the thermal signal(i.e.,temperature)of GNPs within the membrane when irradiated by a laser at the plasmon resonance wavelength of the particle.This process results in higher detection and quantitation of GNPs than in traditional visual detection(i.e.,color intensity).Further,we investigated the effect of laser power(30,100,200 mW),GNP size and shape(30 and 100 nm gold spheres,150 nm gold-silica shells),and laser wavelength(532,800 nm).Applying these innovations to a new TCA LFA design,we demonstrated that 100nm spheres with a 100 mW 532 nm laser provided the best performance(i.e.,LOD=8pg/ml).This LOD is significantly better than that of the current colorimetric LFA and is in the range of the laboratory-based p24 ELISA.In summary,this TCA LFA for p24 protein shows promise for detecting acute HIV infection in POC settings. | Li Zhan Timothy Granade Yilin Liu Xierong Wei Ae Youngpairoj Vickie Sullivan Jeff Johnson John Bischof | 2020 | Microsystems & Nanoengineering2020，6，1： | 3 |
| 3 | MicroRNA expression alterations are linked to tumorigenesis and non-neoplastic processes in pancreatic ductal adenocarcinoma 显示文摘 | Szafranska AE Davison TS John J | 2007 | Oncogene2007，26，30： | 1 |
| 4 | MicroRNA expression alterations are linked to tumorigenesis and non-neoplastic processes in pancreatic ductal adenocarcinoma显示文摘 | Szafranska AE Davison TS John J | 2007 | Oncogene2007，26，： | 1 |
| 5 | MicroRNA expression alterations are hnked to tumorigenesis and non-neoplastic processes in pancreatic ductal adenocarcinoma显示文摘 | Szafranska AE Davison TS John J | | 0，，： | 1 |
| 6 | Preclinical SPECT/CT ima-ging of av(36 integrins for molecular stratification of idiopathic pul-monary fibrosis显示文摘 | John AE Luckett JC Tatler AL | 2013 | J NucI Med2013，54，12： | 1 |
| 7 | MicroRNA expression alterations are linked to tumorigenesis and non-neoplastic processes in pancreatic duetal adcnoearcinoma 显示文摘 | Szafranska AE Davison TS John J | 2007 | Oneogene2007，26，30： | 1 |
| 8 | Preclinical SPECT/CT imaging of av/36 integrins for molecular stratification of idiopathic pulmonaryfibrosis 显示文摘 | John AE Luckett JC Tatler AL | 2013 | J Nucl Med2013，54，12： | 1 |
| 9 | Discovery of a potent nanopartide P-selectin antagonist with anti-inflammatory effects in allergic airway disease 显示文摘 | John AE Lukacs NW Berlin AA | 2003 | FASEB2003，17，15： | 1 |
| 10 | Discovery of a potent nanoparticle P-selectin antagonist with anti-inflammatory effects in allergic airway disease 显示文摘 | John AE Lukacs NW Berlin AA | 2003 | FASEB2003，17，15： | 1 |
| 11 | Discovery of a potent nanoparticle Pselectin antagonist with anti-inflammatory effects in allergic irway disease显示文摘 | John AE Lukacs NW Berlin AA | 2003 | FASEB J2003，17，15： | 1 |
| 12 | Discovery of a potent nanoparticle P-selectin antagonist with anti-inflammatory effects in allergic airway disease显示文摘 | John AE Lukacs NW Berlin AA | 2003 | FASEB2003，17，15： | 1 |
| 13 | Respiratory syncytial virus-induced exaggeration of allergic airway disease is dependent upon CCRl-associated immune responses 显示文摘 | John AE Gerard C J SchaUer M | 2005 | Eur J Immunol2005，35，1： | 1 |
| 14 | The duffy antigen/ receptor for ehemokines exists in an oligomeric form in living cells and functionally antagonizes CCR5 signaling through het ero-oligomerization 显示文摘 | Chakera A Seeber RM John AE et aN | 2008 | Mol Pharmacol2008，73，5： | 1 |
| 15 | MicroRNA expression alterations are linked to tumorigenesis and non-neoplastic processes in pancreatic ductal adenocarcinoma 显示文摘 | Szafranska AE Davison TS John J | 2007 | Oncogene2007，26，30： | 1 |
| 16 | Differential selectivity of cyto-chrome P450 inhibitors against probe substrates in human and ratliver mion>somes 显示文摘 | Victoria AE John FT David JH | 1998 | Br J Clin Pharmacol1998，45，1： | 1 |
| 17 | Dissecting aneurysm of the aorta : a review of 505 eases显示文摘 | Hirst AE Jr Johns VJ Jr Kime SW Jr | 1958 | Medieine1958，37，3： | 1 |
| 18 | Fractalkine has anti-apoptotic and proliferative effects on human vascular smooth muscle cells via epidermal growth factor receptor signalling显示文摘 | White GE Tan TC John AE | 2010 | Cardiovasc Res2010，85，4： | 1 |
| 19 | Dissecting aneurysm of the aorta z A re-view of S05cases显示文摘 | Hirst AE Jr Johns VJ | 1958 | Medicine1958，37，： | 1 |
| 20 | Micro RNA expression alterations are linked to tumorigenesis and non-neoplastic processes in pancreatic ductal adenocarcinoma显示文摘 | Szafranska AE Davison TS John J | 2007 | Oncogene2007，26，30： | 1 |
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