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| 1 | Increased catabolism and decreased unsaturation of ganglioside in patients with inflammatory bowel disease显示文摘AIM: To investigate whether accelerated catabolism of ganglioside and decreased ganglioside content contribute to the etiology of pro-inflammatory intestinal disease. METHODS: Intestinal mucosa from terminal ileum or colon was obtained from patients with ulcerative colitis or inflammatory Crohn's disease(n = 11) undergoing bowel resection and compared to control samples of normal intestine from patients with benign colon polyps(n = 6) and colorectal cancer(n = 12) in this observational case-control study. Gangliosides and phospholipids of intestinal mucosa were characterized by class and ceramide or fatty acid composition using liquid chromatography triple-quad mass spectrometry. Content and composition of ganglioside classes GM1, GM3, GD3, GD1 a, GT1 and GT3 were compared among subject groups. Content and composition of phospholipid classes phosphatidylcholine(PC) and phosphatidylethanolamine were compared among subject groups. Unsaturation index of individual ganglioside and phospholipid classes was computed and compared among subject groups. Ganglioside catabolism enzymes beta-hexosaminidase A(HEXA) and sialidase-3(NEU3) were measured in intestinal mucosa using western blot and compared among subject groups. RESULTS: Relative GM3 ganglioside content was 2-fold higher(P < 0.05) in intestine from patients with inflammatory bowel disease(IBD) compared to control intestine. The quantity of GM3 and ratio of GM3/GD3 was also higher in IBD intestine than control tissue(P < 0.05). Control intestine exhibited 3-fold higher(P < 0.01) relative GD1 a ganglioside content than IBD intestine. GD3 and GD1 a species of ganglioside containing three unsaturated bonds were present in control intestine, but were not detected in IBD intestine. The relative content of PC containing more than two unsaturated bonds was 30% lower in IBD intestine than control intestine(P < 0.05). The relative content of HEXA in IBD intestine was increased 1.7-fold(P < 0.05) and NEU3 was increased 8.3-fold(P < 0.01) compared to normal intestine. Intestinal mucosa in IBD is characterized by increased GM3 content, decreased GD1 a, and a reduction in polyunsaturated fatty acid constituents in GD3, GD1 a and PC.CONCLUSION: This study suggests a new paradigm by proposing that IBD occurs as a consequence of increased metabolism of specific gangliosides. | John J Miklavcic Glen K Shoemaker Vera C Mazurak M Tom Clandinin Tasha DL Hart Kareena L Schnabl Gordon M Lees Bodil MK Larsen Oliver F Bathe Alan BR Thomson M Tom Clandinin | 2015 | World Journal of Gastroenterology2015，21，35： | 3 |
| 2 | Enzyme immunoassay: a new method for the estimation of hemoglobin A1 c 显示文摘 | John WG Gray MR Bates DL | 1993 | Clin Chem1993，39，4： | 1 |
| 3 | Role of ocular ma- trix metalloproteinases in peripheral ulcerative keratitis 显示文摘 | Smith VA John HB Easty DL | 1999 | Br J Ophthamol1999，83，12： | 1 |
| 4 | Nonpeptide angiotensin Ⅱ receptor antagonists: The next generation in antihypertensive therepy显示文摘 | Ruth RW Willian JG John DL | 1996 | J Med Chem1996，39，： | 1 |
| 5 | Management of acute coronary syn-drome in diabetes mellitus显示文摘 | John JM Bhatt DL | 2004 | Herz2004，29，4： | 1 |
| 6 | Enzyme immuno-assay-a new technique for estimating of hemoglobin A1c显示文摘 | John WG Gray MR Bates DL | 1993 | Clin Chem1993，39，4： | 1 |
| 7 | Part 3:evidence evaluation process:2010 international consensus on eardiopulmonary resuscitation and emergency cardiovascular care science with treatment recommendations显示文摘 | Morley PT Atkins DL John E | 2010 | Circulation2010，122，162： | 1 |
| 8 | The German version of the Oral Health Impact Profile - translation and psychometric properties显示文摘 | John MT Patrick DL Slade GD | 2002 | Eur J Oral Sci2002，110，： | 1 |
| 9 | Modification of silk fibroin using diazonium coupling chemistry and the effects on hMSC proliferation and differentiation显示文摘 | Murphy AR St John P Kaplan DL | | 0，，19： | 1 |
| 10 | The german version of the oral health impact profile-translation and psychometric properties 显示文摘 | John MT DL Patrick GD Slade | 2002 | Eur J Oral Sci2002，110，6： | 1 |
| 11 | Apixaban for reduction in stroke and other thromboembolic events in atrial fibrillation (ARISTOTLE) trial: design and rationale显示文摘 | Renato DL John HA Sana MA | 2010 | Am Heart J2010，159，3： | 1 |
| 12 | Acetabulplasty in the treatment of congenital dislocation of the hip显示文摘 | Tervor D Johns DL Fixsen JA | 1975 | J Bone Joint Surg1975，57，： | 1 |
| 13 | Laboratory wash resistance of long-lasting insecticidal nets显示文摘 | John EG Kim AL Mount DL | 2005 | Trop Med Intern Heal2005，10，10： | 1 |
| 14 | Management of Acute Coronary Syndrome in Diabetes ellitus显示文摘 | John JM Bhatt DL | 2004 | Herz2004，29，4： | 1 |
| 15 | Upregulation of endothelial and neuronal constitutive nitric oxide synthase in pregnant rats显示文摘 | XU DL MARTIN PY JOHN ST | 1996 | Am J Physiol Regulatory Integrative Comp Physiol1996，271，： | 1 |
| 16 | Multiplex amplication of STR loci with geneder alleles using infrared fluorescence detection显示文摘 | Reena R John A | 1997 | Forensic Science International1997，85，22： | 1 |
| 17 | Enzyme immunoassay-a new technique for estimating haemoglobin Ale 显示文摘 | John WG Gray MR Bates DL | 1993 | Clin Chem1993，39，4： | 1 |
| 18 | Diphenylamine residues in apples(Malus domestica borkh), cider, and pomace following commercial controlled atmosphere storage显示文摘 | Johns on GD Geronimo J Hughes DL | 1997 | J Agriculture Food Chemistry1997，45，： | 1 |
| 19 | Quality of Life in young people: ratings and factor structure of the Quality of Life Profile Adolescent Version显示文摘 | Bradford R Rutherford DL John A | 2002 | J Adolesc2002，25，3： | 1 |
| 20 | Glomerular development in intraocular and intrarenal grafts of fetal kidneys显示文摘 | John PL Pillion DL | 1991 | Lab Invest1991，64，： | 1 |
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