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| 1 | The Effects of Polyphenol Oxidase and Cycloheximide on the Early Stage of Browning in Phalaenopsis Explants显示文摘Explant browning is one of the major problems in the tissue culture process, and polyphenol oxidase(PPO), is the major proteases involved in plant tissue browning. We investigated the effects of polyphenol oxidase on the early stage of browning in explants of the orchid Phalaenopsis. Our results show that PPO activity was significantly higher in explants cultured for 3 d than in the 0 h control. The levels of PPO transcripts and PPO protein were significantly higher in explants cultured for 6 h compared to the 0 h control; these high expression levels were maintained over increasing cultivation time. Cycloheximide(CHX) treatment reduced PPO transcript levels, PPO protein levels, and PPO enzyme activity. High levels of PPO m RNA and PPO protein were detected in the cytoplasm and vascular bundles of Phalaenopsis explants cultured for 6 h compared to explants cultured for 0 h, 24 h, and 3 d. CHX treatment did not significantly affect the distribution of PPO m RNA and PPO protein in explant tissues, but their levels were significantly lower than those of the untreated control. | XU Chuanjun RU Zhiwei LI Ling ZENG Biyu HUANG Junmei HUANG Wen HU Ou | 2015 | Horticultural Plant Journal2015，1，3： | 2 |
| 2 | Large Scale One-Pot Synthesis of Monodispersed Na_(3)(VOPO_(4))_(2)F Cathode for Na-Ion Batteries显示文摘Na-ion batteries(NIBs)have received significant interest as potential candidates for large-scale energy storage owing to the widespread distribution of sodium and superior low-temperature performance.However,their commercial application is usually hindered by the high production cost and inadequate performance for electrode materials,particularly for cathodes.Na_(3)(VOPO_(4))_(2)F(NVOPF)has been recognized as one of the most promising cathodes for high-energy NIBs owing to the high working voltage and energy density.Here,we report a facile highly efficient room-temperature solution protocol for large-scale synthesis of NVOPF cathode for NIBs.By simply regulating pH,NVOPF can be obtained,which delivered a discharge capacity of 120.2 mAh g^(-1) at 0.1 C and 72%capacity retention over 8000 cycles at 25 C.Besides,the kilogram-level NVOPF products have been synthesized,and 26650 cylindrical cells were fabricated,which exhibit excellent cycling stabilities,remarkable lowtemperature performance with comparable safety features.We hope our findings could provide insights on the industrial application of NVOPF in NIBs. | Qiangqiang Zhang Xing Shen Quan Zhou Kaixuan Li Feixiang Ding Yaxiang Lu Junmei Zhao Liquan Chen Yong-Sheng Hu | 2022 | Energy Material Advances2022，，1： | 2 |
| 3 | Synthesis and Antitumor Activity of 4-tert-Butyl-5-benzyl-2-benzyliminothiazoles显示文摘 | YE Jiao QIU Shenyi HU Aixi PENG Junmei QIN Zhi | 2014 | Chemical Research in Chinese Universities2014，30，1： | 1 |
| 4 | Expanding the gene pool for soybean improvement with its wild relatives显示文摘Genetic diversity is a cornerstone of crop improvement,However,cultivated soybean(Glycine max)has undergone several genetic bottlenecks,including domestication in China,the introduction of landraces to other areas of the world and,latterly,selective breeding,leading to low genetic diversity the poses a major obstacle to soybean improvement.By contrast,there remains a relatively high level of genetic diversity in soybean's wild relatives,especially the perennial soybeans(Glycine subgenus Glycine),which could serve as potential gene pools for improving soybean cultivars.Wild soybeans are phylogenetically diversified and adapted to various habitats,harboring resistance to various biotic and abiotic stresses.Advances in genome and transcriptome sequencing enable alleles associated with desirable traits that were lost during domestication of soybean to be discovered in wild soybean.The collection and conservation of soybean wild relatives and the dissection of their genomic features will accelerate soybean breeding and facilitate sustainable agriculture and food production. | Yongbin Zhuang Xiaoming Li Junmei Hu Ran Xu Dajian Zhang | 2022 | aBIOTECH2022，3，2： | 1 |
| 5 | Subjective well-being, social support, and age-related functioning among the very old in China 显示文摘 | Deng Juelin Hu Junmei Wu Weili | 2010 | Int J Geriatr Psychiatry2010，25，： | 1 |
| 6 | Disodium Terephthalate (Na<sub>2</sub>C<sub>8</sub>H<sub>4</sub>O<sub>4</sub>) as High Performance Anode Material for Low‐Cost Room‐Temperature Sodium‐Ion Battery显示文摘 | Liang Zhao Junmei Zhao Yong‐Sheng Hu Hong Li Zhibin Zhou Michel Armand Liquan Chen | 2012 | Adv Energy Mater2012，，8： | 1 |
| 7 | Effects of electrolyte stratification on performances of AGM valve- regulated lead-acid batteries显示文摘 | Junmei Hu Yonglang Guo | 2007 | Electrochimica Acta2007，52，： | 1 |
| 8 | Investigations on self-discharge of gel valve- regulated lead-acid batteries 显示文摘 | Yonglang Guo Junmei Hu Mingxiang Huang | 2006 | Journal of Power Sources2006，158，： | 1 |
| 9 | Sn doped ZnO layered porous nanocrystals with hierarchical structures and modified surfaces for gas sensors显示文摘 | Mingshui Yao Fei Ding Yuebin Cao Peng Hu Junmei Fan Chen Lu Fangli Yuan Changyong Shi Yunfa Chen | 2014 | Sensors & Actuators: B. Chemical2014，，： | 1 |
| 10 | RF plasma synthesis of nickel nanopowders via hydrogen reduction of nickel hydroxide/carbonate显示文摘 | Bai Liuyang Fan Junmei Hu Peng | | 0，，1： | 1 |
| 11 | RF Plasma Synthesis of Nickel Nanopowders via Hydrogen Reduction of Nickel Hydroxide/Carbonate 显示文摘 | Liuyang Bai Junmei Fan Peng Hu | 2009 | Journal of Alloys and Compounds2009，481，12： | 1 |
| 12 | Effects of platelets on characteristics of lymphocytes cultured in vitro and optimization of adoptive immunotherapy显示文摘T lymphocytes,the main participants of cellular immunity,can express a variety of surface molecules and form different lymphocyte subsets under the induction of different factors to play the functions of immune regulation and immune killing.Studies have shown that platelets play a crucial role in maintaining the stable differentiation of lymphocytes and the balance in immunomodulation.Therefore,it is necessary to study the effect of platelets on lymphocytes in vitro to better understand the role of platelets in the immune system and broaden the application of adoptive immunotherapy.Methods:Cell counting and microscopic observation were used to detect the effect of activated platelets on lymphocyte proliferation in vitro;Flow cytometry was used to detect whether changes in platelet activity affect the proportion of lymphocyte subpopulations in vitro,and to detect differences in the expression of granzyme B;lactate dehydrogenase assay(LDH)was used to determine the difference in lymphocyte killing activity caused by platelet activity in vitro.Results:This was the first to promote lymphocyte proliferation through the expression or release of certain molecules in vitro,demonstrating that platelet activation is one of the key factors.Secondly,activated platelets or inactivated platelets promoted lymphocyte subset differentiation by enhancing the proportion of CD3+CD8+T lymphocytes(CTL cells)but had a slight effect on the proportion of CD3+CD4+T(Th cells)and CD4+CD25+T lymphocytes(Treg cells).Then,it was found that either activated platelets or inactivated platelets down-regulated the proportion of natural killer(NK)T lymphocytes,while activated platelets significantly enhance the proportion of NK lymphocytes.Therefore,by further detecting the killing activity of PBMCs treated with platelets,it was found that activated platelets promoted the extensive anti-tumor activity of lymphocytes and significantly increased the expression of granzyme B.Conclusion:Our results suggest that activated platelets promote lymphocyte proliferation,optimize lymphocyte subpopulation ratio,and promote cytotoxic effect of lymphocytes in vitro,which may provide a new strategy for optimizing the adoptive immunotherapy culture system and improving its efficacy. | CONGLIANG CHEN XIAOHONG LENG YU ZHANG JUNMEI HU DAPENG WEI PEIPEI WANG XIA WANG | 2023 | BIOCELL2023，47，12： | 0 |
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