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| 1 | Protective action of NADPH oxidase inhibitors and role of NADPH oxidase in pathogenesis of colon inflammation in mice显示文摘AIM:To investigate the role of nicotinamide adenine dinucleotide phosphate(NADPH) oxidase in colon epithelial cells in the pathogenesis of acute and chronic colon inflammation in a mouse model of dextran sulphate sodium(DSS)-induced colitis.METHODS:Balb/c mice were divided into three groups:8 mice with acute DSS-induced colitis(3.5% DSS solution;7 d),8 mice with chronic DSS-induced colitis(3.5% DSS solution for 5 d + water for 6 d;4 cycles;total:44 d) and 12 mice without DSS supplementation as a control group.Primary colonic epithelial cells were isolated using chelation method.The cells were cultivated in the presence of mediators(lipopolysaccharide(LPS),apocynin or diphenyleneiodonium).Viability of cells was assessed by fluorescent microscopy.Production of reactive oxygen species(ROS) by the cells was measured fluorometrically using Amplex Red.Production of tumour necrosis factor-alpha(TNF-α) by the colonic epithelial cells was analysed by ELISA.Nox1 gene expression was assessed by real-time PCR.RESULTS:Our study showed that TNF-α level was increased in unstimulated primary colonic cells both in the acute and chronic colitis groups,whereas decreased viability,increased ROS production,and expression of Nox1 was characteristic only for chronic DSS colitis mice when compared to the controls.The stimulation by LPS increased ROS generation via NADPH oxidase and decreased cell viability in mice with acute colitis.Treatment with NADPH oxidase inhibitors increased cell viability and decreased the levels of ROS and TNF-α in the LPS-treated cells isolated from mice of both acute and chronic colitis groups.CONCLUSION:Our study revealed the importance of NADPH oxidase in the pathogenesis of both acute and chronic inflammation of the colon. | Rima Ramonaite Jurgita Skieceviciene Simonas Juzenas Violeta Salteniene Juozas Kupcinskas Paulius Matusevicius Vilmante Borutaite Limas Kupcinskas | 2014 | World Journal of Gastroenterology2014，20，35： | 4 |
| 2 | Atrophic gastritis and gastric cancer tissue miRNome analysis reveals hsa-miR-129-1 and hsa-miR-196a as potential early diagnostic biomarkers显示文摘BACKGROUND Gastric cancer(GC)is one of the most frequently diagnosed tumor globally.In most cases,GC develops in a stepwise manner from chronic gastritis or atrophic gastritis(AG)to cancer.One of the major issues in clinical settings of GC is diagnosis at advanced disease stages resulting in poor prognosis.Micro RNAs(mi RNAs)are small noncoding molecules that play an essential role in a variety of fundamental biological processes.However,clinical potential of mi RNA profiling in the gastric cancerogenesis,especially in premalignant GC cases,remains unclear.AIM To evaluate the AG and GC tissue mi RNomes and identify specific mi RNAs’potential for clinical applications(e.g.,non-invasive diagnostics).METHODS Study included a total of 125 subjects:Controls(CON),AG,and GC patients.All study subjects were recruited at the Departments of Surgery or Gastroenterology,Hospital of Lithuanian University of Health Sciences and divided into the profiling(n=60)and validation(n=65)cohorts.Total RNA isolated from tissue samples was used for preparation of small RNA sequencing libraries and profiled using next-generation sequencing(NGS).Based on NGS data,deregulated mi RNAs hsa-mi R-129-1-3 p and hsa-mi R-196 a-5 p were analyzed in plasma samples of independent cohort consisting of CON,AG,and GC patients.Expression level of hsa-mi R-129-1-3 p and hsa-mi R-196 a-5 p was determined using the quantitative real-time polymerase chain reaction and 2-ΔΔCt method.RESULTS Results of tissue analysis revealed 20 differentially expressed mi RNAs in AG group compared to CON group,129 deregulated mi RNAs in GC compared to CON,and 99 altered mi RNAs comparing GC and AG groups.Only 2 mi RNAs(hsa-mi R-129-1-3 p and hsa-mi R-196 a-5 p)were identified to be step-wise deregulated in healthy-premalignant-malignant sequence.Area under the curve(AUC)-receiver operating characteristic analysis revealed that expression level of hsa-mi R-196 a-5 p is significant for discrimination of CON vs AG,CON vs GC and AG vs GC and resulted in AUCs:88.0%,93.1%and 66.3%,respectively.Comparing results in tissue and plasma samples,hsa-mi R-129-1-3 p was significantly down-regulated in GC compared to AG(P=0.0021 and P=0.024,tissue and plasma,respectively).Moreover,analysis revealed that hsa-mi R-215-3 p/5 p and hsa-mi R-934 were significantly deregulated in GC based on Helicobacter pylori(H.pylori)infection status[log2 fold change(FC)=-4.52,P-adjusted=0.02;log2 FC=-4.00,P-adjusted=0.02;log2 FC=6.09,P-adjusted=0.02,respectively].CONCLUSION Comprehensive mi RNome study provides evidence for gradual deregulation of hsa-mi R-196 a-5 p and hsa-mi R-129-1-3 p in gastric carcinogenesis and found hsami R-215-3 p/5 p and hsa-mi R-934 to be significantly deregulated in H.pylori carrying GC patients. | Greta Varkalaite Evelina Vaitkeviciute Ruta Inciuraite Violeta Salteniene Simonas Juzenas Vytenis Petkevicius Rita Gudaityte Antanas Mickevicius Alexander Link Limas Kupcinskas Marcis Leja Juozas Kupcinskas Jurgita Skieceviciene | 2022 | World Journal of Gastroenterology2022，28，6： | 2 |
| 3 | Polymorphisms of micro RNA target genes IL12B, INSR, CCND1 and IL10 in gastric cancer显示文摘AIM To evaluate associations between mi RNA target genes IL12B,INSR,CCND1 and IL10 polymorphisms and gastric cancer(GC)in European population.METHODS Gene polymorphisms were analyzed in 508 controls and474 GC patients from 3 tertiary centers in Germany,Lithuania and Latvia.Controls were patients from the out-patient departments,who were referred for upper endoscopy because of dyspeptic symptoms and had no history of previous malignancy.Gastric cancer(GC)patients had histopathological verification of gastric adenocarcinoma.Genomic DNA was extracted using salting out method from peripheral blood mononuclear cells.IL12B T>G(rs1368439),INSR T>C(rs1051690),CCND1 A>C(rs7177)and IL10 T>C(rs3024498)SNPs were genotyped by the real-time polymerase chain reaction.Associations between gene polymorphism and GC were evaluated using multiple logistic regression analysis with adjustment for sex,age and country of birth.RESULTS We observed similar distribution of genotypes and allelic frequencies of all polymorphisms between GC patients and controls except of INSR rs1051690.The frequency of the T allele of INSR gene was significantly higher in GC patients than in controls(23.26%and 19.19%respectively,P=0.028).CT genotype was also more prevalent in patients compared to control group(38.48%and 30.12%respectively,P<0.021).Logistic regression analysis revealed that only one polymorphism(rs1051690 in INSR gene)was associated with increased risk of GC.Carriers of CT genotype had higher odds of GC when compared to CC genotype(OR=1.45,95%PI:1.08-1.95,P=0.01).Similar association was observed in a dominant model for INSR gene,where comparison of TT+CT vs CC genotypes showed an increased risk of GC(OR=1.44,95%PI:1.08-1.90,P=0.01).Other analyzed SNPs were not associated with the presence of GC.CONCLUSION INSR rs1051690 SNP is associated with increased risk of GC,while polymorphisms in IL12B,CCND1 and IL10genes are not linked with the presence of GC. | Vytenis Petkevicius Violeta Salteniene Simonas Juzenas Thomas Wex Alexander Link Marcis Leja Ruta Steponaitiene Jurgita Skieceviciene Limas Kupcinskas Laimas Jonaitis Gediminas Kiudelis Peter Malfertheiner Juozas Kupcinskas | 2017 | World Journal of Gastroenterology2017，23，19： | 2 |
| 4 | Quantum dots and nanoparticles for photodynamic and radiation therapies of canc- er显示文摘 | JUZENAS P CHEN W SUN Y P | 2008 | Advanced Drug Delivery Reviews2008，60，15： | 1 |
| 5 | Temperature effect on accumulation of protoporphyrin Ⅸ after topical application of 5-aminolevulinic acid and its methylester and hexylester derivatives in normal mouse skin显示文摘 | Juzenas P Kaalhus O | 2002 | Photochem Photobiol2002，76，： | 1 |
| 6 | Lack of association between miR-27a, miR- 146a, miR- 196a- 2, miR- 492 and miR-608 gene polymorphisms and colorectal cancer显示文摘 | KUPCINSKAS J BRUZAITE I JUZENAS S | 2014 | Sci Rep2014，4，： | 1 |
| 7 | Fluorescence spectroscopy of normal mouse skin exposed to 5-aminolaevulinic acid and red light显示文摘 | Juzenas P Iani V Bagdonas S | 2001 | J Photochem Photobiol B2001，61，12： | 1 |
| 8 | Radiosensitization of tumours by porphyrins显示文摘 | Luksiene Z Juzenas P Moan J | | 0，，01： | 1 |
| 9 | Noninvasive fluorescence excitation spectroscopy during application of 5-aminolevulinic acid in vivo显示文摘 | Juzenas P Juzeniene A Kaalhus O | 2002 | Photochem Photobiol Sci2002，1，10： | 1 |
| 10 | Topical application of5-aminolaevulinic acid,methyl 5-aminolaevulinate and hexyl 5-aminolaevulinate on normal human skin 显示文摘 | Juzeniene A Juzenas P Ma LW | 2006 | Br J Dermatol2006，155，4： | 1 |
| 11 | Topical application of 5-aminolevulinic acid and its methylester,hexylester and octylester derivatives:considerations for dosimetry in mouse skin model显示文摘 | Juzeniene A Juzenas P Iani V | 2002 | Photochem Photobiol2002，76，3： | 1 |
| 12 | Quantum dots and nanoparticles for photodynamic and radiation therapies of cancer显示文摘 | Juzenas P Chen W Sun Y P | 2008 | Advanced Drug Delivery Reviews2008，60，15： | 1 |
| 13 | Systemic photodynamic therapy with aminolevulinic acid induces apoptosis in lesional T lymphocytes of psoriatic plaques显示文摘 | Bissonnette R Tremblay JF Juzenas P | 2002 | J Invest Dermatol2002，119，： | 1 |
| 14 | Topical application of 5-aminolevulinic acid and its methylester, hexylester and octylester derivatives: considerations for dosimetry in mouse skin model显示文摘 | Juzeniene A Juzenas P Iani V | 2002 | Photochem Photobiol2002，76，3： | 1 |
| 15 | Systemic photodynamic therapy with aminolevulinic acid induces apoptosis in lesional T lymphocytes of psoriatic plaques显示文摘 | Tremblay JF Juzenas P | 2002 | J Invest Dermatol2002，119，： | 1 |
| 16 | Systemic photodynamic therapy with aminolevulinic acid induces apoptosis in lesional T lymphocytes of psoriatic plaques显示文摘 | Bissonnette R Tremblay JF Juzenas P | 2002 | J Investigative Dermatol2002，119，： | 1 |
| 17 | Lack of association between miR-27a, miR-146a, miR-196a-2, miR492 and miR-608 gene polymorpbisms and colorectal cancer显示文摘 | Kupcinskas J Bruzaite I Juzenas S | 2014 | Sci Rep2014，4，： | 1 |
| 18 | Prevalence of C282Y, H63D, and S65C mutations in hereditary HFE-hemochromatosis gene in Lithuanian population显示文摘 | Laimutis Kucinskas Simonas Juzenas Jurgita Sventoraityte Ruta Cedaviciute Astra Vitkauskiene Vytenis Kalibatas Jurate Kondrackiene Limas Kupcinskas | 2012 | Annals of Hematology2012，，4： | 1 |
| 19 | Systemic pholodynamic therapy with aminolevulinic acid induces apoptosis in lesional T lymphocyles of psoriatic plaques 显示文摘 | Bissonnelle R Tremblay JF Juzenas P el al | 2002 | J Invest Dermato12002，19，： | 1 |
| 20 | Application of 5-aminolevulinic acid and its derivatives for photodynamic therapy in vitro and in vivo显示文摘 | Juzeniene A Juzenas P Moan J | 2010 | Methods Mol Biol2010，635，： | 1 |
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