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| 1 | Helicobacter pylori vac A genotype is a predominant determinant of immune response to Helicobacter pylori CagA显示文摘AIM To evaluate the frequency of Helicobacter pylori(H. pylori) Cag A antibodies in H. pylori infected subjects and to identify potential histopathological and bacterial factors related to H. pylori Cag A-immune response.METHODS Systematic data to H. pylori isolates, blood samples, gastric biopsies for histological and molecular analyses were available from 99 prospectively recruited subjects. Serological profile(anti-H. pylori, anti-Cag A) was correlated with H. pylori isolates(cag A, EPIYA, vac A s/m genotype), histology(Sydney classification) and mucosal interleukin-8(IL-8) m RNA and protein expression. Selected H. pylori strains were assessed for H. pylori Cag A protein expression and IL-8 induction in co-cultivation model with AGS cells.RESULTS Thirty point three percent of microbiologically confirmed H. pylori infected patients were seropositive for Cag A. Majority of H. pylori isolates were cag A gene positive(93.9%) with following vac A polymorphisms: 42.4% vac A s1m1, 23.2% s1m2 and 34.3% s2m2. Anti-Cag AIg G seropositivity was strongly associated with atrophic gastritis, increased mucosal inflammation according to the Sydney score, IL-8 and cag A m RNA expression. V a c A s a n d m p o l y m o r p h i s m s w e r e t h e m a j o r determinants for positive(vac A s1m1) or negative(vac A s2m2) anti-Cag A serological immune response, which also correlated with the in vitro inflammatory potential in AGS cells. In vitro co-cultivation of representative H. pylori strains with AGS cells confirmed functional Cag A translocation, which showed only partial correlation with Cag A seropositivity in patients, supporting vac A as major co-determinant of the immune response.CONCLUSION Serological immune response to H. pylori cag A + strain in H. pylori infected patients is strongly associated with vac A polymorphism, suggesting the crucial role of bacterial factors in immune and clinical phenotype of the infection. | Alexander Link Cosima Langner Wiebke Schirrmeister Wiebke Habendorf Jochen Weigt Marino Venerito Ina Tammer Dirk Schlüter Philipp Schlaermann Thomas F Meyer Thomas Wex Peter Malfertheiner | 2017 | World Journal of Gastroenterology2017，23，26： | 12 |
| 2 | Different antibiotic susceptibility between antrum and corpus of the stomach,a possible reason for treatment failure of Helicobacter pylori infection显示文摘AIM:To assess whether antibiotic resistance varies between the antrum and corpus of the stomach of patients that are either Helicobacter pylori(H.pylori)therapy-naive or pre-treated.METHODS:H.pylori strains were isolated from antrum and corpus biopsies from 66 patients that received a diagnostic gastroduodenoscopy for variant clinical indications.Antimicrobial susceptibility to amoxicillin,clarithromycin,tetracycline,metronidazole,levofloxacin and rifabutin was tested with the E-test method on IsoSensitest agar with 10 vol%defibrinated horse blood.In patients with a different antibiotic susceptibility pattern between the isolates from the antrum and corpus,DNA fingerprinting via random amplified polymorphic DNA analysis was performed to detect differences among DNA patterns of H.pylori isolates.RESULTS:Primary,secondary and tertiary resistance to clarithromycin was 6.9%,53.8%and 83.3%,retrospectively.Metronidazole and levofloxacin resistance also increased according to the number of previous treatments(17.2%,69.2%,83.3%;13.8%,23.1%,33.3%).Tertiary resistance to rifabutin was detected in12.5%of patients.In none of the 66 patients a resistance against amoxicillin or tetracycline was detectable.Discordant antibiotic susceptibility between antrum and corpus isolates for different antibiotics was seen in 15.2%(10/66)of the patients.Two out of those ten patients were naive to any H.pylori antibiotic treatment.The remaining eight patients previously received at least one eradication therapy.DNA fingerprinting analysis revealed no substantial differences among DNA patterns between antrum and corpus isolates in the majority of patients suggesting an infection with a single H.pylori strain.CONCLUSION:Different antibiotic susceptibility between antrum and corpus biopsies is a common phenomenon and a possible explanation for treatment failure.Resistant H.pylori strains may be missed if just one biopsy from one anatomic site of the stomach is taken for H.pylori susceptibility testing. | Michael Selgrad Ina Tammer Cosima Langner Jan Bornschein Julia Mei?le Arne Kandulski Mariya Varbanova Thomas Wex Dirk Schlüter Peter Malfertheiner | 2014 | World Journal of Gastroenterology2014，20，43： | 10 |
| 3 | Expression of cytokeratins in Helicobacter pylori-associated chronic gastritis of adult patients infected with cagA+strains:An immunohistochemical study显示文摘瞄准:为了与长期的胃炎在成年病人的胃的上皮调查不同 cytokeratins (CK ) 的表示,与 Helicobacter pylori (H pylori ) 感染了 cagA+ 紧张。方法:CK 7 的表示, 8, 18, 19 和 20 在 84 个病人的窦的胃的活体检视组织化学地是学习免疫。所有 CK 是在 cagA+H pylori 胃炎(57 个案例) 染色的免疫, non-H pylori 胃炎(17 个案例) 和正常胃粘膜(10 个案例) 。结果:在 cagA+ H pylori 胃炎, CK8 从表面上皮可比较地被表示到正常的窦粘膜到深腺。CK18 和 CK 19 的分发是未改变的,即表示的 transmucosal,而是紧张在与正常相比的小凹的区域是不同的胃粘膜。Cytokeratin 18 免疫反应在与 H pylori 否定的胃炎和控制相比的 H pylori 积极的胃炎的小凹的上皮是显著地更高的。相反,在 CK19 免疫反应的减少发生在 H pylori 积极的胃炎的小凹的上皮。在没有 H pylori 感染的正常、煽动的窦粘膜, CK20 在表面上皮和上面的小凹的区域强烈 / 中等并且同类地被表示,但是在 H, pylori 导致了胃炎在小凹的区域的表示的重要减少被注意。通常,在正常窦的粘膜和 H pylori 否定的胃炎,, CK7 的表示没被观察在关于半 cagA+ , H 感染 pylori 的病人,节制颈的焦点的 CK7 免疫反应,卷的腺区域被登记,特别在区域与更严重煽动性渗入。结论:在 CK 7 的表示的改变, 18, 19 和 20 在感染 cagA+ 紧张的成年病人和 CK8 的正常表示发生在 H 联系 pylori 的长期的胃炎的窦粘膜。在不同 cytokeratins 表示力量的改变贡献在 H 感染 pylori 的胃粘膜观察的上皮的紧密的连接变弱。 | Vera Todorovic Neda Drndarevic Olivera Mitrovic Institutefor MedicalResearch Aleksandra Sokic-Milutinovic Tomica Milosavljevic Marjan Micev Ivan Nikolic Thomas Wex Peter Malfertheiner | 2006 | World Journal of Gastroenterology2006，12，12： | 2 |
| 4 | Differential Expression of Human Beta Defensin 2 and 3 in Gastric Mucosa of H elicobacter pylori ‐Infected Individuals显示文摘 | Bianca Bauer Thomas Wex Doerthe Kuester Thomas Meyer Peter Malfertheiner | 2012 | Helicobacter2012，，1： | 2 |
| 5 | Polymorphisms of micro RNA target genes IL12B, INSR, CCND1 and IL10 in gastric cancer显示文摘AIM To evaluate associations between mi RNA target genes IL12B,INSR,CCND1 and IL10 polymorphisms and gastric cancer(GC)in European population.METHODS Gene polymorphisms were analyzed in 508 controls and474 GC patients from 3 tertiary centers in Germany,Lithuania and Latvia.Controls were patients from the out-patient departments,who were referred for upper endoscopy because of dyspeptic symptoms and had no history of previous malignancy.Gastric cancer(GC)patients had histopathological verification of gastric adenocarcinoma.Genomic DNA was extracted using salting out method from peripheral blood mononuclear cells.IL12B T>G(rs1368439),INSR T>C(rs1051690),CCND1 A>C(rs7177)and IL10 T>C(rs3024498)SNPs were genotyped by the real-time polymerase chain reaction.Associations between gene polymorphism and GC were evaluated using multiple logistic regression analysis with adjustment for sex,age and country of birth.RESULTS We observed similar distribution of genotypes and allelic frequencies of all polymorphisms between GC patients and controls except of INSR rs1051690.The frequency of the T allele of INSR gene was significantly higher in GC patients than in controls(23.26%and 19.19%respectively,P=0.028).CT genotype was also more prevalent in patients compared to control group(38.48%and 30.12%respectively,P<0.021).Logistic regression analysis revealed that only one polymorphism(rs1051690 in INSR gene)was associated with increased risk of GC.Carriers of CT genotype had higher odds of GC when compared to CC genotype(OR=1.45,95%PI:1.08-1.95,P=0.01).Similar association was observed in a dominant model for INSR gene,where comparison of TT+CT vs CC genotypes showed an increased risk of GC(OR=1.44,95%PI:1.08-1.90,P=0.01).Other analyzed SNPs were not associated with the presence of GC.CONCLUSION INSR rs1051690 SNP is associated with increased risk of GC,while polymorphisms in IL12B,CCND1 and IL10genes are not linked with the presence of GC. | Vytenis Petkevicius Violeta Salteniene Simonas Juzenas Thomas Wex Alexander Link Marcis Leja Ruta Steponaitiene Jurgita Skieceviciene Limas Kupcinskas Laimas Jonaitis Gediminas Kiudelis Peter Malfertheiner Juozas Kupcinskas | 2017 | World Journal of Gastroenterology2017，23，19： | 2 |
| 6 | Serological assessment of gastric mucosal atrophy in gastric cancer显示文摘 | Jan Bornschein Michael Selgrad Thomas Wex et : | 2012 | BMC Gastroenterology2012，12，10： | 1 |
| 7 | Interleukin-1B and interleukin-1 receptor antagonist gene polymorphisms are not associated with premalignant gastric conditions: a combined haplotype analysis显示文摘 | Limas Kupcinskas Thomas Wex Juozas Kupcinskas Marcis Leja Audrius Ivanauskas Laimas Virgilijus Jonaitis Dainius Janciauskas Gediminas Kiudelis Konrads Funka Agnese Sudraba Han-Mo Chiu Jaw-Town Lin Peter Malfertheiner | 2010 | European Journal of Gastroenterology & Hepatology2010，，10： | 1 |
| 8 | Antibiotic susceptibility of Helicobacter pylori in central Germany and its relationship with the number of eradication therapies显示文摘 | Michael Selgrad Julia Meile Jan Bornschein Arne Kandulski Cosima Langner Mariya Varbanova Thomas Wex Ina Tammer Dirk Schlüter Peter Malfertheiner | 2013 | European Journal of Gastroenterology & Hepatology2013，，11： | 1 |
| 9 | H. pylori Infection Is a Key Risk Factor for Proximal Gastric Cancer显示文摘 | Jan Bornschein Michael Selgrad Maren Warnecke Doerthe Kuester Thomas Wex Peter Malfertheiner | 2010 | Digestive Diseases and Sciences2010，，11： | 1 |
| 10 | A Frequent Toll‐Like Receptor 1 Gene Polymorphism Affects NK ‐ and T‐cell IFN ‐γ Production and is Associated with Helicobacter pylori ‐induced Gastric Disease显示文摘 | Chin‐An Yang Carmen Scheibenbogen Sandra Bauer Christoph Kleinle Thomas Wex Jan Bornschein Peter Malfertheiner Stephan Hellmig Ralf R. Schumann Lutz Hamann | 2012 | Helicobacter2012，，1： | 1 |
| 11 | Esophageal Intraluminal Baseline Impedance Differentiates Gastroesophageal Reflux Disease From Functional Heartburn显示文摘 | Arne Kandulski Jochen Weigt Carlos Caro Doerthe Jechorek Thomas Wex Peter Malfertheiner | 2014 | Clinical Gastroenterology and Hepatology2014，，： | 1 |
| 12 | Antibiotic susceptibility of Helicobacter pylori in central Germany and its relationship with the number of eradication therapies显示文摘 | Michael Selgrad Julia Meile Jan Bornschein Arne Kandulski Cosima Langner Mariya Varbanova Thomas Wex Ina Tammer Dirk Schlüter Peter Malfertheiner | 2013 | European Journal of Gastroenterology & Hepatology2013，，11： | 1 |
| 13 | A Frequent Toll‐Like Receptor 1 Gene Polymorphism Affects NK ‐ and T‐cell IFN ‐γ Production and is Associated with Helicobacter pylori ‐induced Gastric Disease显示文摘 | Chin‐An Yang Carmen Scheibenbogen Sandra Bauer Christoph Kleinle Thomas Wex Jan Bornschein Peter Malfertheiner Stephan Hellmig Ralf R. Schumann Lutz Hamann | 2012 | Helicobacter2012，，1： | 1 |
| 14 | Helicobacter pylori infection and fundic gastric atrophy are not associated with esophageal squamous cell carcinoma: a case–control study显示文摘 | Marino Venerito Sabine Kohrs Thomas Wex Daniela Adolf Doerthe Kuester Daniel Schubert Ulrich Peitz Klaus M?nkemüller Peter Malfertheiner | 2011 | European Journal of Gastroenterology & Hepatology2011，，10： | 1 |
| 15 | 1047 Efficacy of an Investigational Recombinant Antigen Based Vaccine Against a CagA H. pylori Infectious Challenge in Healthy Volunteers显示文摘 | Peter Malfertheiner Michael Selgrad Thomas Wex Jan Bornschein Emanuela Palla Giuseppe Del Giudice David Graham Penny M. Heaton | 2012 | Gastroenterology2012，，5： | 1 |
| 16 | Plasma leptin and leptin receptor expression in childhood acute lymphoblastic leukemia显示文摘 | Heike Wex Edita Ponelis Thomas Wex Regina Dressend?rfer Uwe Mittler Peter Vorwerk | 2002 | International Journal of Hematology2002，，5： | 1 |
| 17 | Helicobacter pylori but not gastrin is associated with the development of colonic neoplasms显示文摘 | Michael Selgrad Jan Bornschein Arne Kandulski Carla Hille Jochen Weigt Albert Roessner Thomas Wex Peter Malfertheiner | 2014 | Int. J. Cancer2014，，5： | 1 |
| 18 | A Frequent Toll‐Like Receptor 1 Gene Polymorphism Affects NK ‐ and T‐cell IFN ‐γ Production and is Associated with Helicobacter pylori ‐induced Gastric Disease显示文摘 | Chin‐An Yang Carmen Scheibenbogen Sandra Bauer Christoph Kleinle Thomas Wex Jan Bornschein Peter Malfertheiner Stephan Hellmig Ralf R. Schumann Lutz Hamann | 2012 | Helicobacter2012，，1： | 1 |
| 19 | 幽门螺杆菌下调人胃黏膜分泌性白细胞蛋白酶抑制因子的表达显示文摘目的通过低剂量阿斯匹林的处理作为炎性模型,研究分析人胃和十二指肠黏膜分泌性白细胞蛋白酶抑制因子(SLPI)表达下调是否与幽门螺杆菌(Helicobacter prlori,Hp)定居或感染有关.方法选择20例健康志愿者分成Hp+和Hp-组,其中Hp+组成功进行了根除治疗.每组10人,每人口服阿司匹林100 mg/d.通过活检提取受试者不同部位胃黏膜的总RNA和蛋白,采用RT/realtime PCR检测SLPI的mRNA表达水平和单抗ELISA定量测定SLPI蛋白,统计分析SLPI基因表达的相关数据.结果与Hp-组比较,Hp+组胃窦黏膜SLPI表达水平显著降低,但Hp+组经抗生素根除治疗后其SLPI水平恢复至与Hp-组相当的水平.在低剂量阿司匹林处理下,所有受试者表现组织学观察到的活动性或慢性炎性征.各组在药物处理的不同时间虽有一定的差异,但与药物处理对照组(第0天)比较,SLPI的表达差异没有统计学意义[第1天:(77±880)pg/10?g蛋白;第3天:(941±149)pg/10?g蛋白;第7天:(763±363)pg/10?g蛋白].阿司匹林处理的1周内以胃窦为主的胃炎持续存在(活动性:1.5~1.7;慢性:1.2~2.1),但与Hp-和Hpe(根除组)比较,Hp+组的胃窦黏膜SLPI蛋白表达仍显著降低.结论低剂量阿司匹林处理所致炎性反应对人胃黏膜SLPI的表达没有影响,胃窦黏膜SLPI基因表达的下调与Hp感染有关. | 叶嗣颖 Thomas Wex Gerhard Treiber Michael Vieth Peter Malfertheiner | 2005 | 中华微生物学和免疫学杂志2005，25，10： | 0 |
| 20 | Dongting Lake显示文摘Located along the middle reaches of the Yangtze River,Dongting ALake at its peak stretched to a total area of 6,000 square kilometres(sq.km).Historical records say that the lake was once part of the ancient Yunmeng Lakes that spanned modern-day Hunan and Hubei provinces,and covered a total of 40,000 sq.km.Later,with the silting up of the Yangtze River,the Yunmeng Lakes were divided into northern and southern parts.The northern part,located north of the Yangtze River,became a marsh.The southern part,to the south of the Yangtze,remained a lake,which came to be called Dongting Lake. | Zhang Wexing Thomas Price | 2021 | Beijing2021，，40： | 0 |
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