|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A Duplex Real-Time PCR Assay for the Simultaneous Detection of Porcine Circovirus 2 and Circovirus 3显示文摘Porcine circoviruses(PCV) include PCV1, PCV2, and the new-emerging PCV3. PCV2 is pathogenic to pigs, but the pathogenicity of PCV3 in pigs is debatable. Recently, there have been frequent reports of PCV2 and PCV3 co-infections in clinical samples. Thus, it would be practical to develop a duplex PCR method to detect PCV2 and PCV3 simultaneously. In this study, specific primers and probes were designed to target PCV2 cap and PCV3 rep genes. A duplex real-time PCR method was then developed to detect the two viruses. The assay was found to be highly specific, sensitive, and reproducible for PCV2/3 without cross-reactions with other swine pathogens. The sensitivity of this assay was 2.9 copies for the PCV2 plasmid and 22.5 copies for the PCV3 plasmid. The established assay was then used to detect PCV2/3 infection in 340 clinical samples collected in the first half of 2017. The results showed that the co-infection rate of PCV2/3 in the samples was 27.6%. Our study provides an important tool that can be used to perform urgently needed surveys for the two porcine circoviruses to evaluate their impact on the swine industry. | Xiangdong Li Mingming Qiao Ming Sun Kegong Tian | 2018 | Virologica Sinica2018，33，2： | 15 |
| 2 | Higher alcohol synthesis over Cu-Fe composite oxides with high selectivity to C_(2+)OH显示文摘Cu-Fe composite oxides were prepared by co-precipitation method and tested for higher alcohol synthesis from syngas. The selectivity to C2+OH and C6+OH in alcohol distribution was very high while the methane product fraction in hydrocarbon distribution was rather low, demonstrating a promising potential in higher alcohols synthesis from syngas. The distribution of alcohols and hydrocarbons approximately obeyed Anderson-Schulz-Flory distribution with similar chain growth probability, indicating alcohols and hydrocarbons derived from the same intermediates. The effects of Cu/Fe molar ratio, reaction temperature and gas hourly space velocity (GHSV) on catalytic performance were studied in detail. The sample with a Cu/Fe molar ratio of 10/1 exhibited the best catalytic performance. Higher reaction temperature accelerated water-gas-shift reaction and led to lower total alcohols selectivity. GHSV showed great effect on catalytic performance and higher GHSV increased the total alcohol selectivity, indicating there existed visible dehydration reaction of alcohol into hydrocarbon. | Zhenghong Bao Kang Xiao Xingzhen Qi Xinxing Wang Liangshu Zhong Kegong Fang Minggui Lin Yuhan Sun | 2013 | Journal of Energy Chemistry2013，22，1： | 10 |
| 3 | Non-harmonious deformation controlling of gob-side entry in thin coal seam under dynamic pressure显示文摘The behavior of gob-side entry under dynamic pressure is totally different from the one driven after the movement of overlying strata above the adjacent coalface goaf.The gob-side entry will experience severe roof lateral structural adjustments caused by adjacent coalface mining.Thus the deformation and failure characteristics of narrow coal pillar along the gob should be carefully considered.On the basis of the data of the gob-side entry obtained in a thin coal seam under dynamic pressure,the measures to reinforce the narrow coal pillar are put forward.In addition,the non-harmonious controlling of the rock structures and non-equilibrium gob-side entry deformation is proposed to avoid potential failure.Field practices show that the supporting problems of the gob-side entry under dynamic pressure can be well addressed,which could be used in other similar mining cases. | Kegong Fan Hongguang Liang Chishuai Ma Chuanwei Zang | 2014 | Journal of Rock Mechanics and Geotechnical Engineering2014，6，3： | 5 |
| 4 | MicroRNA-21 acts as an oncomir through multiple targets in human hepatocellular carcinoma显示文摘 | Changzheng Liu Jia Yu Shuangni Yu Robert M. Lavker Lei Cai Wei Liu Kegong Yang Xiaodong He Songsen Chen | 2010 | Journal of Hepatology2010，，1： | 2 |
| 5 | A comprehensive analysis of the Bencao(herbal)small RNA Atlas reveals novel RNA therapeutics for treating human diseases显示文摘Cross-kingdom herbal mi RNA was first reported in 2012.Using a modified herbal extraction protocol,we obtained 73,677,287sequences by RNA-seq from 245 traditional Chinese Medicine(TCM),of which 20,758,257 were unique sequences.We constructed a Bencao(herbal)small RNA(s RNA)Atlas(http://bencao.bmicc.cn),annotated the sequences by sequence-based clustering,and created a nomenclature system for Bencao s RNAs.The profiles of 21,757 mi RNAs in the Atlas were highly consistent with those of plant mi RNAs in mi RBase.Using software tools,our results demonstrated that all human genes might be regulated by s RNAs from the Bencao s RNA Atlas,part of the predicted human target genes were experimentally validated,suggesting that Bencao s RNAs might be one of the main bioactive components of herbal medicines.We established roadmaps for oligonucleotide drugs development and optimization of TCM prescriptions.Moreover,the decoctosome,a lipo-nano particle consisting of 0.5%–2.5%of the decoction,demonstrated potent medical effects.We propose a Bencao(herbal)Index,including small-molecule compounds(SM),protein peptides(P),nucleic acid(N),non-nucleic and non-proteinogenic large-molecule compounds(LM)and elements from Mendeleev's periodic table(E),to quantitatively measure the medical effects of botanic medicine.The Bencao s RNA Atlas is a resource for developing gene-targeting oligonucleotide drugs and optimizing botanical medicine,and may provide potential remedies for the theory and practice of one medicine. | Yinghao Cao Yexuan Lin Na Sun Xinyi Du Yixin Dong Song Mei Xingyu Deng Xiaobei Li Shaoting Guo Kegong Tang Jiaqi Liu Xiangyu Qiao Dandan Zhao Yuhao Qin Cong Zhang Tianyi Xin Xiaohu Shi Congzhao Zhou Tao Dong De-an Guo Benedikt MKessler Dong Xu Jingyuan Song Fengming Huang Xiaoyue Wang Chengyu Jiang | 2023 | Science China(Life Sciences)2023，66，10： | 2 |
| 6 | Molecular Epidemiology of Porcine Circovirus Type 3 Infection in Swine Herds in China显示文摘Dear Editor,Porcine circovirus type 3(PCV3)is a new-emerging circovirus belonging to the genus Circovirus in the family Circoviridae in which PCV type 1(PCV1)and PCV type 2(PCV2)were well documented(Palinski et al.2017).PCV1 is a cell culture-derived virus and is considered to be nonpathogenic for swine,whereas PCV2 is the primary etiological agent of porcine circovirus-associated | Xiangdong Li Chaolin Zhang Mingming Qiao Jiangsong Guo Guangyuan Xing Chunxia Jins Juan Wang Ming Sun Kegong Tian | 2018 | Virologica Sinica2018，33，4： | 2 |
| 7 | Research and development of a novel subunit vaccine for the currently circulating pseudorabies virus variant in China显示文摘Pseudorabies(PR)is a devastating viral disease which leads to fatal encephalitis and respiratory disorders in pigs.Commercial gE-deleted live pseudorabies virus(PRV)vaccine has been widely used to control this disease in China.However,the new-emerging variants of PRV compromises the protection provided by current vaccines and lead to the outbreak of PR in vaccinated pig herds.Several killed and live vaccine candidates based on current PRV variants have been reported to be effective to control the disease.A subunit vaccine based on gB protein,one major PRV glycoprotein which elicits strong humoral and cellular immune responses,however,was never evaluated for protection against the current circulating PRV variants.In this study,full-length PRV gB protein was successfully expressed in baculovirus/insect cells in the soluble format and was tested on 3-week-old piglets as a subunit vaccine.Compared with unvaccinated pigs,the gB-vaccinated pigs developed specific antibody-mediated responses and were protected from the virulent PRV HN1201 challenge.All vaccinated pigs survived without showing any PRV-specific respiratory and neurological signs,but all unvaccinated pigs died within 7 days after HN1201 challenge.Hence,this novel gB-based vaccine could be applied as an effective subunit vaccine to control PRV variant in China. | Yuzhou WANG Tongyan WANG He YAN Fanli YANG Linghua GUO Qingyuan YANG Xule HU Feifei TAN Yan XIAO Xiangdong LI Kegong TIAN | 2015 | Frontiers of Agricultural Science and Engineering2015，2，3： | 2 |
| 8 | Synthesis of Acetic Acid on Pd-H_4SiW_(12)O_(40)-Based Catalysts by Direct Oxidation of Ethylene显示文摘Synthesis of acetic acid by direct oxidation of ethylene on Pd-H4SiW12O4o-based catalystswas studied in a fixed-bed integral reactor and a pulse differential reactor. From the performance of thecatalysts with different compositions and configurations, it is proposed that acetic acid is predominantlyproduced via an intermediate of acetaldehyde. This can be easily confirmed by comparing the productdistributions in the integral and the differential reactors. The active sites for acetic acid formation areconsidered to exist mainly at the boundaries between the H4SiW12O4o and the Pd particles. The Pd-basedcatalysts reduced by H2/N2 have higher activities than those reduced by hydrazine, as explained by thedegree of Pd dispersion obtained from the characteristics of hydrogen chemical adsorption. It was foundthat the Pd-Se-SiW12/SiO2 catalyst with selenium tetrachloride as a precursor was more active than thatwith potassium selenite, and that the acetic acid yield can be greatly increased by adding a suitable amountof dichloroethane (C2H4Cl2/C2H4 mole ratio=0.03) to the reactants. | Xinping Wang Kegong Fang Jianlu Zhang Tianxi Cai | 2002 | Journal of Natural Gas Chemistry2002，11，1： | 2 |
| 9 | H5N6禽流感病毒的基因起源、进化和流行显示文摘2014~2016年间对我国16个省份活禽市场的监测,共鉴定了3174份流感阳性样品,分离并测序了1135份禽流感病毒,分属于31种亚型。 | yuhai bi quanjiao chen qianli wang jianjun chen tao jin gary wong chuansong quan jun liu jun wu renfu yin lihua zhao mingxin li zhuang ding rongrong zou wen xu hong li huijun wang kegong tian guanghua fu yu huang alexander shestopalov shoujun li bing xu hongjie yu tingrong luo lin lu xun xu yang luo 刘映霞 史卫峰 刘翟 高福 | 2017 | 科学新闻2017，0，4： | 2 |
| 10 | Topology group concept for truss topology optimization with frequency constraints显示文摘 | Xu Bin Jiang Jiesheng Tong Weihua Wu Kegong | 2003 | Journal of Sound and Vibration2003，261，2003： | 1 |
| 11 | Orally administered BZL-s RNA-20 oligonucleotide targeting TLR4 effectively ameliorates acute lung injury in mice显示文摘The global COVID-19 pandemic emerged at the end of December 2019.Acute respiratory distress syndrome(ARDS)and acute lung injury(ALI)are common lethal outcomes of bacterial lipopolysaccharide(LPS),avian influenza virus,and SARS-Co V-2.Toll-like receptor 4(TLR4)is a key target in the pathological pathway of ARDS and ALI.Previous studies have reported that herbal small RNAs(s RNAs)are a functional medical component.BZL-s RNA-20(Accession number:B59471456;Family ID:F2201.Q001979.B11)is a potent inhibitor of Toll-like receptor 4(TLR4)and pro-inflammatory cytokines.Furthermore,BZLs RNA-20 reduces intracellular levels of cytokines induced by lipoteichoic acid(LTA)and polyinosinic-polycytidylic acid(poly(I:C)).We found that BZL-s RNA-20 rescued the viability of cells infected with avian influenza H5N1,SARS-Co V-2,and several of its variants of concern(VOCs).Acute lung injury induced by LPS and SARS-Co V-2 in mice was significantly ameliorated by the oral medical decoctosome mimic(bencaosome;sphinganine(d22:0)+BZL-s RNA-20).Our findings suggest that BZLs RNA-20 could be a pan-anti-ARDS/ALI drug. | Dandan Zhao Yuhao Qin Jiaqi Liu Kegong Tang Shuaiyao Lu Zirui Liu Yexuan Lin Cong Zhang Fengming Huang Jiahui Chang Chang Li Mingyao Tian Yiming Ma Xiaoyun Li Congzhao Zhou Xiao Li Xiaozhong Peng Ningyi Jin Chengyu Jiang | 2023 | Science China(Life Sciences)2023，66，7： | 1 |
| 12 | Cloning and Expression of Human Stem Cell Factor Fused with Thrombopoietin Mimetic Peptide in Escherichia coli显示文摘 | Lin Su Songsen Chen Kegong Yang Changzheng Liu Zhiquan Liang | 2006 | Biotechnology Letters2006，，12： | 1 |
| 13 | Emergence of Fatal PRRSV Variants: Unparalleled Outbreaks of Atypical PRRS in China and Molecular Dissection of the Unique Hallmark 显示文摘 | Kegong Tian Xiuling Yu Tiezhu Zhao | 2007 | PloSone2007，6，： | 1 |
| 14 | Emergence of Fatal PRRSV Variants: Unparalleled Outbreaks of Atypical PRRS in China and Molecular Dissection of the Unique Hall- mark显示文摘 | Kegong Tian Xiuling Yu Tiezhu Zhao | 2007 | PLoS ONE2007，，6： | 1 |
| 15 | Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark 显示文摘 | Tian Kegong Yu Xiuling Zhao Tiezhu | 2007 | PLoS ONE2007，，6： | 1 |
| 16 | Emergence of Fatal PRRSV Variants: Unparalleled Outbreaks of Atypical PRRS in China and Molecular Dissection of the Unique Hallmark显示文摘 | KEGONG TIAN XIULING YU | 2007 | Plos One2007，，6： | 1 |
| 17 | Effect of nickel precursors on the performance of Ni/AlMCM-41 catalysts for n-dodecane hydroconversion显示文摘 | FANG Kegong REN Jie SUN Yuhan | 2005 | J Mol Catal A:Chem2005，229，： | 1 |
| 18 | A Short Re-view of Heterogeneous Catalytic Process for Mixed Alcohols Synthesis via Syngas显示文摘 | Fang Kegong Lin Debao Lin Minggui | 2009 | Catal Today2009，147，2： | 1 |
| 19 | Carbon dis- persed iron manganese catalyst for light olefin synthesis from CO hydrogenation显示文摘 | Zhang Jianli Fang Kegong Zhang Kan | 2009 | Korean J Chem Eng2009，26，： | 1 |
| 20 | Emergence of Fatal PRRSV Variants: Unparalleled Outbreaks of Atypical PRRS in China and Molecular Dissection of the Unique Hallmark 显示文摘 | Kegong Tian Xiuling Yu Tiezhu Zhao | 2007 | PLoS ONE2007，2，6： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员