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| 1 | Therapeutic potential and pharmacological activities of Atractylodes lancea (Thunb.) DC.显示文摘The rhizome of Atractylodes Iancen(A.lancea)(Thunb.) DC.(AL)is extensively used in Chinese,Thai,and Japanese traditional medicines as crude extracts/decoctions or a component in various herbal formulations.Various pharmacological activities of Al.and its major constituents have been demonstrated in ritro.ex ciro.and in animal models.Results from the toxicity studies in animal models suggest safety profile of AL,and its active constituents.Despite extensive use with positive impression in many diseases,there has not been a clinical study that can conclusively support its efficacy and safely profile in human.This review comprehensively summarizes current information on the pharmacological activities of AL and their active constituents including anticancer,anti-inflammatory,antimicrobial and antipyretic activities,as well as activities on central nervous,cardiovascular,and gastrointestinal systems. | Nut Koonrungsesomboon Kesara Na-Bangchang Juntra Karbwang | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，6： | 29 |
| 2 | Polymorphisms of the oxidant enzymes glutathione S-transferase and glutathione reductase and their association with resistance of Plasmodium falciparum isolates to antimalarial drugs显示文摘Objective:To investigate the association between amplification of the two regulatory genes controlling glutathione(GSH) levels,glutathione reductase(PfGR) and glutathione S-transferase (PfGST) genes and sensitivity of Plasmodium falciparum(P.falciparum) isolates collected from different malaria endemic areas of Thailand to standard antimalarial drugs.Methods:A total of 70 P.falciparum isolates were collected from endemic areas of multi-drug resistance (Tak,Chantaburi and Ranong Provinces) during the year 2008-2009.The in vitro assessment of antimalarial activity of P.falciparum clones(K1- and Dd2 chloroquine resistant and 3D7- chloroquine sensitive) and isolates to chloroquine,quinine,mefloquine and arteusnate was performed based on SYBR Green modified assay.Results:68(97.14%),11(15.71%) and 28(40%) isolates respectively were classified as chloroquine-,quinine- and mefloquine-resistant isolates. With this limited number of P.falciparum isolates included in the analysis,no significant association between amplification of PfGST gene and sensitivity of the parasite to chloroquine, quinine,mefloquine and quinine was found.Based on PCR analysis,Dd2,Kl and 3D7 clones all contained only one copy of the PfGST gene.All isolates(70) also carried only one copy number of PfGST gene.There appears to be an association between amplification of PfGR gene and chloroquine resistance.The 3D7 and Dd2 clones were found to carry only one PfGR gene copy, whereas the K1 clone carried two gene copies.Conclusions:Chloroquine resistance is likely to be a consequence of multi-factors and enzymes in the GSH system may be partly involved. Larger number of parasite isolates are required to increase power of the hypothesis testing in order to confirm the involvement of both genes as well as other genes implicated in glutathione metabolism in conferring chloroquine resistance. | Raewadee Wisedpanichkij Wanna Chaicharoenkul Poonuch Mahamad Prapichaya Prompradit Kesara Na-Bangchang | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，9： | 3 |
| 3 | In vitro inhibitory effects of plumbagin,the promising antimalarial candidate,on human cytochrome P450 enzymes显示文摘Objective:To investigate the propensity of plumbagin to inhibit the three isoforms of human cytochrome P450(CYP),ie.,CYP1A2,CYP2C19,and CYP3A4 using human liver microsomes in ritro.Methods:Inhibitory effects of plumbagin on the three human CYP isoformswere investigated using pooled human liver microsomes.Phenacetin O-deethylation,omeprazole hydroxylation and nifedipine oxidation were used as selective substrates for CYP1A2,CYP2C19 and CYP3A4 activities,respectively.Concentrations of paracetamol,5-hydroxyomeprazole,and oxidized nifedipine were determined in microsomal incubation mixture using high performance liquid chromatography.Results:Plumbagin showed significantinhibitory effects on all CYP isoforms.but with the most potent activity on CYP2C19-mediated omeprazole hydroxylation.The IC50(concentration that inhibits enzyme activity by 50%) values of plumbagin and nootkatone(selective inhibitor) for CYP2C19 were(0.78±0.01) and(27.31±0.66) μM,respectively.The inhibitory activities on CYP1 A2-mediated phenacetin O-deethylation and CYP3A4-mediated nifedipine oxidation were moderate.The IC_(50) values of plumbagin and-naphthoflavone(selective inhibitor) for CYP1A2 were(1.39±0.01) and(0.02±.0.36) μM,respectively.The corresponding IC_(50) values of plumbagin and ketoconazole(selective inhibitor) for CYP3A4 were(2.37+0.10) and(0.18±0.06) μM,respectively.Conclusions:Clinical relevance of the interference of human drug metabolizing enzymes should be aware of for further development scheme of plumbagin as antimalarial drug when used in combination with other antimalarial drugs which are metabolized by these CYP isoforms. | Wiriyaporn Sumsakul Wanna Chaijaroenkul Kesara Na-Bangchang | 2015 | Asian Pacific Journal of Tropical Medicine2015，8，11： | 2 |
| 4 | Assessment of in vitro sensitivity of Plasmodium vivax fresh isolates显示文摘Objective:To compare the applicability of the SYBK Grcen-Ⅰ assay with the standard schizont maturalion assay,for determination of sensitivity of Plasmodium vivax(P.vivax) to chloroquine and a new antifolale WR 99210.Methods:The study was conducted at Mae Tao Clinic for migrant workers,Tak Province during April 2009 to July 2010.A total of 64 blood samples(1 mL blood collected into sodium heparinized plastic tube) were collected from patients with monoinfection with P.vivax malaria prior to treatment with standard regimen of a 3-day chloroquine. In vitro sensitivity of P.vivax isolates was evaluated by schizont maturation inhibition and SYBR Green-Ⅰ assays.Results:A total of 30 out of 64 blood samples collected from patients with P.vivax malaria were successfully analyzed using both the microscopic schizont maturation inhibition and SYBR Green-I assays.The failure rates of the schizont maturation inhibition assay(50%) and the SYBR Green-I assay(54%) were similar(P=0.51).The median IC_(10)s,IC_(50)s and IC_(90)s of both chloroquine and WR99210 were not significantly different from the clinical isolates of P.vivax tested.Based on the cut-off of 100 nM,the prevalences of chloroquine resistance determined by schizont maturation inhibition and SYBR Green-I assays were 19 and 11 isolates,respectively.The strength of agreement between the two methods was very poor for both chloroquine and WR992I0.Conclusions:On the basis of this condition and its superior sensitivity,the microscopic method appears better than the SYUK Green-I Green assay for assessing in vitro sensitivity of fresh P.vivax isolates to antimalarial drugs. | Poonuch Muhamad Wanna Chacharoenkul Kanchana Rungsihirunrat Ronnatrai Ruengweerayut Kesara NaBangchang | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，1： | 2 |
| 5 | Antimalarial activity and toxicity of Garcinia mangostana Linn.显示文摘Objective:To investigate the antimalarial activity and toxicity of the crude ethanolic extract of its pericarp both in vitro and in vim.Methods:The antimalarial activity of Gareinja mangostana(G.mangostana)Linn.extract against 3D7 and Kl Plasmodium falciparum(P.falciparum)clone were assessed using SYBR green I-based assay.A 4-day suppressive test of Plasmodium berghei{P.berghei)infected mouse was performed to investigate in vivo antimalarial activity.Results:The in vitro antimalarial activity was seleclive(SI>5?and classified as weak and good lo moderate activity against both 3D7 and K1 P.falciparum,clones with median IC_(50)(range)values of 11.12(10.94-11.29)and 7.54(6.80-7.68)μg/mL,respectively.The extract was considered nontoxic to mice.The maximum tolerated doses for acute and subacute toxicity in mice were 5 000and 2 000 mg/kg,respectively.Median(range)parasite density on day 4 of the negative control group(25%Tween-80),mice treated with 250,500,1000,and 2 000 mg/kg body weight of the extract,and 10 mg/kg body weight of chloroquine for 14 d were 12.8(12.2-13.7),11.4(9.49-13.8),11.6(9.9-12.5),11.7(10.6-12.8),10.9(9.4-11.6)and 0(0-0)%respectively.Parasite density on day 4in the control group treated with Tween-80 was higher than the groups treated with chloroquine and all dose levels of the extract.Conclusions:G.mangostana linn,showed weak antimalarial activity of the extract both in vitro and in vivo could be due to limitation of absorption of the active compounds. | Ratchanu Bunyong Wanna Chaijaroenkul Tullayakorn Plengsuriyakarn Kesara Na-Bangchang | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，9： | 2 |
| 6 | Genomic and genetic relationships among species of Leymus (Poaceae:Triticeae) inferred from 18S-26S ribosomal genes显示文摘 | Kesara A J | 2001 | American Journal of Botany2001，88，4： | 1 |
| 7 | Antimalarial activities of medicinal plants and herbal formulations used in Thai traditional medicine显示文摘 | Artitaya Thiengsusuk Wanna Chaijaroenkul Kesara Na-Bangchang | 2013 | Parasitology Research2013，，4： | 1 |
| 8 | Discovery of the genus Pseudoroegneria (Triticeae, Poaceae)in the Western Mediterranean on exploring the generic boundaries of Elymus显示文摘In this study,we review the classification of two species,Elymus hispanicus and E.marginatus,which are restricted to highly valuable and sensitive Mediterranean ecosystems.The genomic composition of the two species is analysed by in situ hybridization.In addition,Iodicule morphology and foliar anatomy of both species are compared with those of E.caninus,E.repens,E.sibiricus (i.e.,the type species of Elymus s.s.)and Pseudoroegneria strigosa (i.e.,the type species of Pseudoroegneria).The genomic formula 2n =8x =56;HStStSt is proposed for E.hispanicus and 2n =4x =28;StSt for E.marginatus.In this latter species,the absence of the ribosomal genes in one of the two St genomes suggests that diploidization may have occurred during the evolution of the species. Regarding foliar anatomy,E.hispanicus,E.caninus,E.repens,and E.sibiricus shared several characteristics,but the leaf blades of E.marginatus proved anatomically more similar to those of Ps.strigosa.The data complied support the contention that:(i)E.hispanicus belongs to Elymus s.s.;(ii)E.marginatus should be transferred to Pseudoroegneria;and (iii)the morphology of the Iodicules should be carefully reconsidered for appropriately describing the boundaries between Elymus s.s.and Pseudoroegneria.The new combination Ps.marginata is proposed and a detailed iconography of the plant is provided. | Victor Lucia M.Montserrat Martinez-Ortega Enrique Rico Kesara Anamthawat-Jonsson | 2019 | Journal of Systematics and Evolution2019，57，1： | 1 |
| 9 | A brief review on biomarkers and proteomic approach for malaria research显示文摘Malaria remains as one of the significant health threat to people living in countries throughout tropical and subtropical zones.Proteomic studies of Plasmodium,the protozoan causing malaria,is essential for understanding its cellular structure,growth stage-specific expression of protein metabolites and complex interaction with host.In-depth knowledge of the pathogen is required for identification of novel biomarkers that can be utilized to develop diagnostic tests and therapeutic antimalarial drugs.The alarming rise in drug-resistant strains of Plasmodium has created an urgent need to identify new targets for drug development that can act by obstructing life cycle of this parasite.In the present review,we briefly discuss on role of various biomarkers including Plnsmodium-assaciated aldolase,histidine-rich proteins and lactate dehydrogenase for diagnosis of malaria.Here we also summarize the present and future prospects of currently used techniques in proteomic approaches such as two dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight(MALDI-TOF)for diagnosis and potential identification of novel proteins for malaria research. | Vivek Bhakta Mathema Kesara Na-Bangchang | 2015 | Asian Pacific Journal of Tropical Medicine2015，8，4： | 1 |
| 10 | Inhibitory activities of plumbagin on cell migration and invasion and inducing activity on cholangiocarcinoma cell apoptosis显示文摘Objective: To investigate the cytotoxic, apoptotic and inhibitory activities on cell migration and invasion of plumbagin in the human cholangiocarcinoma(CCA) cell line(CL-6) in comparison with human embryonic fibroblast cell line(OUMS). Methods: Cytotoxicity activity was evaluated using MTT assay. Inhibitory effect on cell migration and invasion were investigated using label-free real-time cell analysis and QCM ECMatrix cell invasion chamber, respectively. Apoptotic activity was evaluated using flow cytometry and Cell Event? Caspase 3/7 assay. Results: Based on results of the cytotoxicity test in CL-6 cells, 50% inhibitory concentration(IC_(50), Mean±SD) values of plumbagin and the standard drug 5-fluorouracil were(24.00±3.33) and(1 036.00±137.77) μmol/L, respectively. The corresponding values for OUMS cells were(57.00±5.23) and(2 147.00±209.98) μmol/L, respectively. The selectivity index was 2.28. The inhibitory activities of plumbagin on cell migration and invasion were potent and concentration-dependent with IC_(50) of 25.0 μmol/L and complete inhibition at 25.0 μmol/L. Flow cytometry analysis showed that plumbagin at 12.5 μmol/L(half IC_(50)) induced CL-6 cell apoptosis(43.24% of control) through stimulation of caspase 3/7 activities. Complete cell apoptosis was observed at 12.5 μmol/L. Conclusions: The cytotoxic activity and inhibition of migration and invasion including apoptosis induction in the human CCA cell line(CL-6) suggest that plumbagin could be a promising candidate for CCA chemotherapeutics. However, its relatively low selective cytotoxic effect on CCA cells is a major concern. | Luxsana Panrit Tullayakorn Plengsuriyakarn Pongsakorn Martviset Kesara Na-Bangchang | 2018 | Asian Pacific Journal of Tropical Medicine2018，11，7： | 1 |
| 11 | Possible role of PGD_2 in malaria infections显示文摘Objective:To preliminarily investigate the possible role of prostaglandin D_2(PGD_2) in malaria infections.Methods:Blood and urinary samples(n=120 each) were collected from Thai patients with Plasmodium falciparum(P.falciparum) with moderate(n=26) and high(n=4) parasitemia,patients with Plasmodium vivax(P.vivax)(n=30),patients with fever associated with other infections(n=30),and healthy subjects(n=30).PGD_2 concentrations in plasma and urinary samples of healthy subjects,patients with fever associated with other infections and patients with malaria were determined using Prostaglandin D2-MOX express EIA kit(Cayman Chemical,USA).Results:The possible association between PGD_2 and malaria infections is clearly demonstrated with PGD_2 concentration in urine.The urinary PGD_2 concentrations were relatively high(about 5-fold) in patients with P.falciparum with moderate parasitemia and P.vivax infections compared with other groups.Furthermore,the concentration in patients with P.falciparum with moderate parasitemia and P.vivax infection were significantly higher than that in healthy subjects and patients with fever associated with other infections.Conclusions:Urinary PGD_2 concentrations may offer a more dependable and useful tool for predicting malaria severity.Confirmation is this preliminary finding is required with a larger sample size. | Pimwan Thongdee Jiraporn Kuesap Raewadee Wisedpanichkij Kesara Na-Bangchang | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，9： | 1 |
| 12 | Glycoproteomics analysis of plasma proteins associated with Opisthorchis viverrini infection-induced cholangiocarcinoma in hamster model显示文摘Objective: To apply lectin affinity chromatography and glycoproteomics-based LC-MS/MS to preliminarily investigate the possible potential plasma biomarkers of Opisthorchis viverrini(OV)-associated CCA in OV/dimethylnitrosamine(DMN)-induced CCA hamster model. Methods: Nine Syrian hamsters were divided into 3 groups as follows(n=3 each): normal(healthy control group); OV group; and OV/DMN group(CCA group). Pooled plasma samples collected from animals in each group at the 6th month post-infection with OV metacercarae were subjected to glycoproteomics analysis. Glycoproteins in the pooled sample from each group were initially isolated by concanavilin A(Con A)-based affinity chromatography. The expression of glycoproteins isolated by both enrichment methods were determined using LCMS/MS. Results: Among the 24 Con A-binding glycoproteins isolated, two proteins, N-myc downstream regulated gene 1(NDRG1) and fetuin-B(FETUB) were found up-regulated only in the samples from the OV and control groups, but not in the OV/DMN(CCA) groups. On the other hand, one protein, i.e., NSFL1 cofactor p47 isoform x3(NSFL1C) was found only in the samples from OV/DMN(CCA) and control groups, but not in the OV group. The remaining 21 proteins were upregulated in the samples from all groups. Conclusions: NDRG1, FETUB and NSFL1 C glycoproteins isolated by Con A-based affinity chromatography could be potential biomarkers for CCA. Plasma samples with negative for NDRG1 and FETUB proteins but positive for NSFL1 C are likely to be OV-associated CCA. Nevertheless, this conclusion remains to be confirmed whether this battery test can discriminate OV-associated CCA from other risk factors. | Pramote Sriwanitchrak Atchara Paemanee Sittiruk Roytrakul Vithoon Viyanant Kesara Na-Bangchang | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，12： | 1 |
| 13 | Isolation,characterization,and analysis of Leymus-specific DNA sequences显示文摘 | SIGRIDUR K B KESARA A J | 2003 | Genome2003，46，： | 1 |
| 14 | DNA fingerprinting of Populus trichocarpa clones using RAPD markers显示文摘 | Vignir Sigurdsson Kesara Anamthawat Jonsson Adalsteinn Sigurgeirsson | 1995 | New Forests1995，10，3： | 1 |
| 15 | Development of oral pharmaceutical formulation of standardized crude ethanolic extract of Atractylodes lancea(Thunb) DC.显示文摘Cholangiocarcinoma(CCA), the adenocarcinoma of the biliary duct, is commonly reported in Asia with the highest incidence in northeastern Thailand. Chemotherapy of this type of cancer is limited due to the lack of effective chemotherapeutic drugs. A series of previous studies support further research and development of Atractylodes lancea(Thunb) DC.(AL) as a potential candidate for the treatment of CCA as a crude ethanolic extract. In the present study, we aimed to develop an oral pharmaceutical formulation(capsule) of the standardized AL crude ethanolic extract for further clinical development in patients with CCA. Major steps included macroscopic and microscopic authentication of the AL rhizomes, preparation of standardized AL extract, preparation of oral pharmaceutical formulation(capsule) of the standardized AL extract, quantitative and qualitative analysis of the marker compound(atractylodin) in the formulated AL extract, evaluation of contaminations of heavy metals, pesticides residues, and microorganisms in the ground AL rhizomes and the formulated(capsule) powder of AL, physicochemical and pharmaceutical properties of the formulated AL extract/capsule, and cytotoxicity evaluation of the formulated AL extract. Results of all evaluations confirmed satisfactory pharmaceutical properties of oral(capsule) formulation of the standardized AL extract. | Thananchanoke Rattanathada Tullayakorn Plengsuriyakarn Rathapon Asasujarit Anurak Cheoymang Juntra Karbwang Kesara Na-Bangchang | 2020 | Journal of Chinese Pharmaceutical Sciences2020，29，4： | 1 |
| 16 | Clinical-parasitological response and in-vitro sensitivity of Plasmodium vivax to chloroquine and quinine on the western border of Thailand显示文摘 | Oumaporn Tasanor Ronnatrai Ruengweerayut Jeerapat Sirichaisinthop Kanungnit Congpuong Walther H. Wernsdorfer Kesara Na-Bangchang | 2005 | Transactions of the Royal Society of Tropical Medicine and Hygiene2005，，5： | 1 |
| 17 | An in vitro system for assessing the sensitivity of Plasmodium vivax to chloroquine显示文摘 | Oumaporn Tasanor Harald Noedl Kesara Na-Bangchang Kanungnit Congpuong Jeeraphat Sirichaisinthop Walther H Wernsdorfer | 2002 | Acta Tropica2002，，1： | 1 |
| 18 | Chromosome number variation and polyploidy in 19 Kaempferia (Zingiberaceae) taxa from Thailand and one species from Laos显示文摘几 Kaempferia 种,对泰国地方性,对保存地位稀罕、因此命名;另外的种类广泛地被栽培。我们进行了这个类的广泛的细胞发生的调查阐明这些植物的植物、分类的描述。学习包括了从老挝在整个泰国,和一种从区域属于 15 Kaempferia 种类和四 undescribed taxa 的 42 就职。我们从从发芽根茎前 situ 收集的根尖端房间决定了染色体数字,但是在收集从的花检验了成熟分裂野。有丝分裂的分析证实 2n 染色体数字从 22 (双, 15 taxa ) , 33 (三倍性,三种) , 44 (tetraploid,五 taxa ) 到 55 (pentaploid,一种) 。四 taxa 与不同 ploidy 层次包括了就职。meiotic 分析证明所有 14 双就职调查了显示的正常成熟分裂,形成 11 bivalents,显示为这个类的基础染色体数字 x=11。Meiotic 数字从三倍性的和四 tetraploid 就职被获得。三倍性显示出 11 trivalents,最可能的显示 autotriploidy。二 tetraploid 就职给由 22 bivalents 组成的常规 meiotic 人物看了,可能显示异源从种间的混血儿发源,与植物形态学的观察一致的一个假设。另外的二 tetraploid 就职属于主要不规则的 meiotic 考虑的一样的种类和表演。细胞发生的信息为在类评估富饶和杂种是有用的。好种子集合被观察在之中双并且 tetraploid 就职。三倍性并且在另一方面, pentaploid 植物不设置种子,但是生产生长地繁殖的根茎的大簇。 | Nattapon Nopporncharoenkul Jatuporn Chanmai Thaya Jeniittikul Kesara Anamthawat-Jonsson Puangpaka Soontornchainaksaeng | 2017 | Journal of Systematics and Evolution2017，55，5： | 1 |
| 19 | SYBR GREEN I AND TAQMAN QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION METHODS FOR THE DETERMINATION OF AMPLIFICATION OF PLASMODIUM FALCIPARUM MULTIDRUG RESISTANCE-1 GENE (PFMDR1)显示文摘 | Muhamad Poonuch Chaijaroenkul Wanna Congpuong Kanungnit Na-Bangchang Kesara | 2011 | EN2011，，5： | 1 |
| 20 | Molecular analysis of pfatp6 and pfmdr1 polymorphisms and their association with in vitro sensitivity in Plasmodium falciparum isolates from the Thai-Myanmar border显示文摘 | Papichaya Phompradit Raewadee Wisedpanichkij Poonuch Muhamad Wanna Chaijaroenkul Kesara Na-Bangchang | 2011 | Acta Tropica2011，，1： | 1 |
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