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| 1 | 不同澄清剂对金樱子发酵果酒澄清效果的影响显示文摘以果胶酶、明胶、膨润土、壳聚糖四种澄清剂单一或者复合使用的处理方法对金樱子发酵果酒进行澄清处理,测定了金樱子果酒澄清处理前后的透光率、酒精度、总糖、总酸以及相应感官评价等指标。实验结果表明:以复合澄清剂澄清效果最佳,即明胶0.02g/L、膨润土1.2g/L和壳聚糖0.4g/L,经澄清处理后,果酒的理化性质变化小,酒精度为14.2%vol,总糖4.5g/L,总酸2.8g/L,透光率96.5%,处理后的果酒呈宝石光泽,果香、酒香良好、酒体醇厚,典型优雅。 | 谢晶 陈跃进 Wanna Ammawath 夏永良 金晨钟 肖荣 高进 | 2013 | 食品工业科技2013，34，4： | 26 |
| 2 | Ultrasound-assisted Low Density Solvent Based Dispersive Liquid-liquid Microextraction for Determination of Phthalate Esters in Bottled Water Samples显示文摘 | UANSIRI Sutthirak VICHAPONG Jitlada KANCHANAMAYOON Wanna | 2016 | Chemical Research in Chinese Universities2016，32，2： | 5 |
| 3 | Polymorphisms of the oxidant enzymes glutathione S-transferase and glutathione reductase and their association with resistance of Plasmodium falciparum isolates to antimalarial drugs显示文摘Objective:To investigate the association between amplification of the two regulatory genes controlling glutathione(GSH) levels,glutathione reductase(PfGR) and glutathione S-transferase (PfGST) genes and sensitivity of Plasmodium falciparum(P.falciparum) isolates collected from different malaria endemic areas of Thailand to standard antimalarial drugs.Methods:A total of 70 P.falciparum isolates were collected from endemic areas of multi-drug resistance (Tak,Chantaburi and Ranong Provinces) during the year 2008-2009.The in vitro assessment of antimalarial activity of P.falciparum clones(K1- and Dd2 chloroquine resistant and 3D7- chloroquine sensitive) and isolates to chloroquine,quinine,mefloquine and arteusnate was performed based on SYBR Green modified assay.Results:68(97.14%),11(15.71%) and 28(40%) isolates respectively were classified as chloroquine-,quinine- and mefloquine-resistant isolates. With this limited number of P.falciparum isolates included in the analysis,no significant association between amplification of PfGST gene and sensitivity of the parasite to chloroquine, quinine,mefloquine and quinine was found.Based on PCR analysis,Dd2,Kl and 3D7 clones all contained only one copy of the PfGST gene.All isolates(70) also carried only one copy number of PfGST gene.There appears to be an association between amplification of PfGR gene and chloroquine resistance.The 3D7 and Dd2 clones were found to carry only one PfGR gene copy, whereas the K1 clone carried two gene copies.Conclusions:Chloroquine resistance is likely to be a consequence of multi-factors and enzymes in the GSH system may be partly involved. Larger number of parasite isolates are required to increase power of the hypothesis testing in order to confirm the involvement of both genes as well as other genes implicated in glutathione metabolism in conferring chloroquine resistance. | Raewadee Wisedpanichkij Wanna Chaicharoenkul Poonuch Mahamad Prapichaya Prompradit Kesara Na-Bangchang | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，9： | 3 |
| 4 | A Thai family with hereditary pancreatitis and increased cancer risk due to a mutation in PRSS1 gene显示文摘AIM: To investigate mutation of serine protease 1-cationic trypsinogen (CT, PRSS1) gene in members of a Thai family with hereditary pancreatitis and pancreatic cancer.METHODS: Polymerase chain reaction and direct sequencing were performed to analyze the PRSS1 gene in two members of the family affected by pancreatitis.Allele specific amplification (ASA) method was then developed to detect the mutation of the PRSS1 gene in all available members of the family and normal control subjects.RESULTS: A cytosine (C) to thymine (T) mutation at position 2441 (g.2441C>T) of the PRSS1 gene, which results in a substitution of arginine by cysteine at position 116 (R116C) of CT, was identified by direct sequencing in both clinically affected members of the family but was not found in the unaffected member. This mutation, which might be arising from deamination of methylated cytosine in CpG dinucleotide of codon 116 (CGT>FGT), was also detected by the ASA method in the two affected members and a proband's brother but was not observed in unaffected members and 54 normal control subjects.CONCLUSION: Autosomal dominant pancreatitis with increased cancer risk in the studied Thai family is most likely due to missense (R116C) mutation in the PRSS1gene. | Theeraphong Pho-Iam Wanna Thongnoppakhun Pa-Thai Yenchitsomanus Chanin Limwongse | 2005 | World Journal of Gastroenterology2005，11，11： | 3 |
| 5 | In vitro inhibitory effects of plumbagin,the promising antimalarial candidate,on human cytochrome P450 enzymes显示文摘Objective:To investigate the propensity of plumbagin to inhibit the three isoforms of human cytochrome P450(CYP),ie.,CYP1A2,CYP2C19,and CYP3A4 using human liver microsomes in ritro.Methods:Inhibitory effects of plumbagin on the three human CYP isoformswere investigated using pooled human liver microsomes.Phenacetin O-deethylation,omeprazole hydroxylation and nifedipine oxidation were used as selective substrates for CYP1A2,CYP2C19 and CYP3A4 activities,respectively.Concentrations of paracetamol,5-hydroxyomeprazole,and oxidized nifedipine were determined in microsomal incubation mixture using high performance liquid chromatography.Results:Plumbagin showed significantinhibitory effects on all CYP isoforms.but with the most potent activity on CYP2C19-mediated omeprazole hydroxylation.The IC50(concentration that inhibits enzyme activity by 50%) values of plumbagin and nootkatone(selective inhibitor) for CYP2C19 were(0.78±0.01) and(27.31±0.66) μM,respectively.The inhibitory activities on CYP1 A2-mediated phenacetin O-deethylation and CYP3A4-mediated nifedipine oxidation were moderate.The IC_(50) values of plumbagin and-naphthoflavone(selective inhibitor) for CYP1A2 were(1.39±0.01) and(0.02±.0.36) μM,respectively.The corresponding IC_(50) values of plumbagin and ketoconazole(selective inhibitor) for CYP3A4 were(2.37+0.10) and(0.18±0.06) μM,respectively.Conclusions:Clinical relevance of the interference of human drug metabolizing enzymes should be aware of for further development scheme of plumbagin as antimalarial drug when used in combination with other antimalarial drugs which are metabolized by these CYP isoforms. | Wiriyaporn Sumsakul Wanna Chaijaroenkul Kesara Na-Bangchang | 2015 | Asian Pacific Journal of Tropical Medicine2015，8，11： | 2 |
| 6 | Assessment of in vitro sensitivity of Plasmodium vivax fresh isolates显示文摘Objective:To compare the applicability of the SYBK Grcen-Ⅰ assay with the standard schizont maturalion assay,for determination of sensitivity of Plasmodium vivax(P.vivax) to chloroquine and a new antifolale WR 99210.Methods:The study was conducted at Mae Tao Clinic for migrant workers,Tak Province during April 2009 to July 2010.A total of 64 blood samples(1 mL blood collected into sodium heparinized plastic tube) were collected from patients with monoinfection with P.vivax malaria prior to treatment with standard regimen of a 3-day chloroquine. In vitro sensitivity of P.vivax isolates was evaluated by schizont maturation inhibition and SYBR Green-Ⅰ assays.Results:A total of 30 out of 64 blood samples collected from patients with P.vivax malaria were successfully analyzed using both the microscopic schizont maturation inhibition and SYBR Green-I assays.The failure rates of the schizont maturation inhibition assay(50%) and the SYBR Green-I assay(54%) were similar(P=0.51).The median IC_(10)s,IC_(50)s and IC_(90)s of both chloroquine and WR99210 were not significantly different from the clinical isolates of P.vivax tested.Based on the cut-off of 100 nM,the prevalences of chloroquine resistance determined by schizont maturation inhibition and SYBR Green-I assays were 19 and 11 isolates,respectively.The strength of agreement between the two methods was very poor for both chloroquine and WR992I0.Conclusions:On the basis of this condition and its superior sensitivity,the microscopic method appears better than the SYUK Green-I Green assay for assessing in vitro sensitivity of fresh P.vivax isolates to antimalarial drugs. | Poonuch Muhamad Wanna Chacharoenkul Kanchana Rungsihirunrat Ronnatrai Ruengweerayut Kesara NaBangchang | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，1： | 2 |
| 7 | 国内外细支卷烟设计参数对比及国内细支烟用卷烟纸特性分析显示文摘对比国内外细支烟设计参数差异,对国内细支烟主要设计特点及其卷烟纸特性进行研究。开展实验设计,对开放吸阻、封闭吸阻等烟支设计参数,通风率等滤棒设计参数和透气度、助燃剂含量等卷烟纸设计参数进行测定,表明国内细支烟采用相对较高的卷烟纸透气度、助燃剂含量和烟支吸阻,以及相对较低的滤棒通风率。在烟支和滤棒方面:1)开放吸阻主要分布在1 200~1 600 Pa;2)滤棒通风率主要分布在24%~48%,与焦油含量相关。根据卷烟纸特性的分析提出相关建议:1)选用全麻卷烟纸有利于突现烟草本香;2)适当提高滤嘴通风率、降低卷烟纸透气度至50 CU或40 CU,有潜力提高卷烟抽吸前后的一致性;3)尽可能选择低定量的卷烟纸,以降低对烟草本香的影响;4)助燃剂以混合柠檬酸盐为主,以减少'掉火头'现象。 | 王小平 周桂园 吴雄会 陈泽亮 黄启志 肖翠翠 陈绍全 李锐 周俊 刘静 WANNA Joseph 陈才 | 2018 | 工业技术创新2018，5，3： | 2 |
| 8 | Antimalarial activity and toxicity of Garcinia mangostana Linn.显示文摘Objective:To investigate the antimalarial activity and toxicity of the crude ethanolic extract of its pericarp both in vitro and in vim.Methods:The antimalarial activity of Gareinja mangostana(G.mangostana)Linn.extract against 3D7 and Kl Plasmodium falciparum(P.falciparum)clone were assessed using SYBR green I-based assay.A 4-day suppressive test of Plasmodium berghei{P.berghei)infected mouse was performed to investigate in vivo antimalarial activity.Results:The in vitro antimalarial activity was seleclive(SI>5?and classified as weak and good lo moderate activity against both 3D7 and K1 P.falciparum,clones with median IC_(50)(range)values of 11.12(10.94-11.29)and 7.54(6.80-7.68)μg/mL,respectively.The extract was considered nontoxic to mice.The maximum tolerated doses for acute and subacute toxicity in mice were 5 000and 2 000 mg/kg,respectively.Median(range)parasite density on day 4 of the negative control group(25%Tween-80),mice treated with 250,500,1000,and 2 000 mg/kg body weight of the extract,and 10 mg/kg body weight of chloroquine for 14 d were 12.8(12.2-13.7),11.4(9.49-13.8),11.6(9.9-12.5),11.7(10.6-12.8),10.9(9.4-11.6)and 0(0-0)%respectively.Parasite density on day 4in the control group treated with Tween-80 was higher than the groups treated with chloroquine and all dose levels of the extract.Conclusions:G.mangostana linn,showed weak antimalarial activity of the extract both in vitro and in vivo could be due to limitation of absorption of the active compounds. | Ratchanu Bunyong Wanna Chaijaroenkul Tullayakorn Plengsuriyakarn Kesara Na-Bangchang | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，9： | 2 |
| 9 | Facial nerve outcomes in facial nerve schwannomas显示文摘 | MCRACKAN T R RIVAS A R WANNA G B | 2011 | Otol Neurotol2011，33，： | 1 |
| 10 | Middle ear implantable hearing devices:an overview显示文摘 | Haynes D S Young J A Wanna G B | 2009 | Trends Amplif2009，13，3： | 1 |
| 11 | Antimalarial activities of medicinal plants and herbal formulations used in Thai traditional medicine显示文摘 | Artitaya Thiengsusuk Wanna Chaijaroenkul Kesara Na-Bangchang | 2013 | Parasitology Research2013，，4： | 1 |
| 12 | Sequence variations of the first ribosomal intemal transcribed spacer of Penaeus species in Thailand显示文摘 | Wanna W Chotigeat W Phongdara A | 2006 | J Exp Mar Biol Ecol2006，331，1： | 1 |
| 13 | Spinal Versus Epidural Anesthesia for Cesarean Delivery in Severe Preeclampsia: A Prospective Randomized, Multicenter Study显示文摘 | Shusee Visalyaputra Oraluxna Rodanant Wanna Somboonviboon Kamthorn Tantivitayatan Somboon Thienthong Wanawimol Saengchote | 2005 | Anesthesia & Analgesia2005，，3： | 1 |
| 14 | Exploiting the dual functions of polymer depressants in fine particle flotation显示文摘 | Liu Q Wannas D Peng Y J | 2006 | International Journal of Mineral Processing2006，80，24： | 1 |
| 15 | “Not Like the Good Old Days”:The Political Impact of the Fitzgerald Inquiry into Police Corruption in Queensland显示文摘 | Coaldrake P Wanna J | | Australian Quarterly0，60，4： | 1 |
| 16 | Simultaneous manipulation of the nitric o:dde and prostanoid pathways reduces myocardial reperfusion injury 显示文摘 | Wanna FS Obayashi DY Young JN | 1995 | J Thorac Cardiovasc Surg1995，110，41： | 1 |
| 17 | Molecular cloning and expression of caspase from white shrimp Penaeus merguiensis显示文摘 | Phongdara A Wanna W Chotigeat W | 2006 | Aquaculture2006，252，24： | 1 |
| 18 | Glomerular epithelial cells transform to myofibroblasts: early but not late removal of TGF-betal reverses transformation 显示文摘 | Sam R Wanna L Gudehithlu KP | 2006 | Transl Res2006，148，3： | 1 |
| 19 | Specific intronic p53 mutation in esophageal squamous cell carcinoma in Southern Thailand显示文摘AIM:To investigate p53 mutations in esophageal cancer in a high-risk population,and correlate them with smoking,alcohol consumption and betel chewing.METHODS:One hundred and sixty-five tumor samples of esophageal squamous cell carcinoma(ESCC) obtained from a university hospital in Songkhla province,Southern Thailand were investigated for p53 mutations in exons 5-8,using polymerase chain reaction-single strand conformation polymorphism analysis,followed by direct sequencing.A polymerase chain reactionrestriction fragment length polymorphism(RFLP) assay was additionally used to confirm possible germline mutation in intron 6.A history of risk habits was obtained by interviews.The association between risk habits and mutation frequency was evaluated using the χ 2 test.RESULTS:The studied specimens were from 139 male and 26 female patients with ESCC,treated at Songklanagarind Hospital.Most of the patients were smokers(86.7%) and alcohol consumers(72.73%),and 38.3% were betel chewers.Forty-three mutations of the p53 gene were detected in 25.5%(42/165) of tumor samples.Mutations were most commonly found in exon 5(25.6%) and exon 8(25.6%).Mutations in the hot-spot codon 248 were found in four cases(9.3% of all mutations).G:C→C:G(30.23%),G:C→A:T(27.90%) and G:C →T:A(16.28%) were the prevalent spectra of mutations.Unexpectedly,among 10 intronic mutations,eight cases harbored a similar mutation:G→C substitution in intron 6(nucleotide 12759,GenBank NC_000017).These were additionally confirmed by the RFLP technique.Similar mutations were also detected in their matched blood samples using RFLP and direct sequencing,which suggested germline mutations.There was no significant correlation between risk habits and p53 mutation frequency.CONCLUSION:A proportion of Thai ESCC patients harbored specific intronic p53 mutations,which might be germline mutations.Further studies are needed to explore this novel finding. | Paramee Thongsuksai Pleumjit Boonyaphiphat Puttisak Puttawibul Wanna Sudhikaran | 2010 | World Journal of Gastroenterology2010，16，42： | 1 |
| 20 | Interleukin-12 expression enhances vesicular stomatitis virus oncolytie therapy in murine squamous cell car cinoma显示文摘 | SHIN E J WANNA G B CHOI B | 2007 | Laryngoscope2007，117，2： | 1 |
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