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3篇 您的检索式:作者名="Kim Boutilier"
    题名 作者 年代 出处 被引量
1Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum显示文摘Doubled haploid(DH) technology is used to obtain homozygous lines in a single generation, a technique that significantly accelerates the crop breeding trajectory. Traditionally, in vitro culture is used to generate DHs, but this technique is limited by species and genotype recalcitrance. In vivo haploid induction(HI) through seed is widely and efficiently used in maize and was recently extended to several other crops. Here we show that in vivo HI can be triggered by mutation of DMP maternal haploid inducer genes in allopolyploid(allotetraploid) Brassica napus and Nicotiana tabacum. We developed a pipeline for selection of DMP orthologs for clustered regularly interspaced palindromic repeats mutagenesis and demonstrated average amphihaploid induction rates of2.4% and 1.2% in multiple B. napus and N. tabacum genotypes, respectively. These results further confirmed the HI ability of DMP gene in polyploid dicot crops. The DMP-HI system offers a novel DH technology to facilitate breeding in these crops. The success of this approach and the conservation of DMP genes in dicots suggest the broad applicability of this technique in other dicot crops.Yu Zhong Yuwen Wang Baojian Chen Jinchu Liu Dong Wang Mengran Li Xiaolong Qi Chenxu Liu Kim Boutilier Shaojiang Chen 2022Journal of Integrative Plant Biology2022,64,6:4
2Bnm1, a Brassica pollen-specific gene显示文摘Brian K. Treacy Jiro Hattori Isabelle Prud’homme Eric Barbour Kim Boutilier Chris L. Baszczynski Bin Huang Douglas A. Johnson Brian L. Miki 1997Plant Molecular Biology1997,,4:1
3Ectopicexpression of baby boom triggers a conversion from vegetative toembryonic growth显示文摘Boutilier Kim Offringa Remko Sharma Vijay K 2002The Plant Cell2002,14,8:1
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