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| 1 | Perspectives of CRISPR/Cas-mediated cisengineering in horticulture:unlocking the neglected potential for crop improvement显示文摘Directed breeding of horticultural crops is essential for increasing yield,nutritional content,and consumer-valued characteristics such as shape and color of the produce.However,limited genetic diversity restricts the amount of crop improvement that can be achieved through conventional breeding approaches.Natural genetic changes in cisregulatory regions of genes play important roles in shaping phenotypic diversity by altering their expression.Utilization of CRISPR/Cas editing in crop species can accelerate crop improvement through the introduction of genetic variation in a targeted manner.The advent of CRISPR/Cas-mediated cis-regulatory region engineering(cis-engineering)provides a more refined method for modulating gene expression and creating phenotypic diversity to benefit crop improvement.Here,we focus on the current applications of CRISPR/Cas-mediated cis-engineering in horticultural crops.We describe strategies and limitations for its use in crop improvement,including de novo cis-regulatory element(CRE)discovery,precise genome editing,and transgene-free genome editing.In addition,we discuss the challenges and prospects regarding current technologies and achievements.CRISPR/Cas-mediated cis-engineering is a critical tool for generating horticultural crops that are better able to adapt to climate change and providing food for an increasing world population. | Qiang Li Manoj Sapkota Esther van der Knaap | 2020 | Horticulture Research2020，7，1： | 3 |
| 2 | Identification and characterization of GLOBE, a major gene controlling fruit shape and impacting fruit size and marketability in tomato显示文摘Within large-fruited germplasm,fruit size is influenced by flat and globe shapes.Whereas flat fruits are smaller and retain better marketability,globe fruits are larger and more prone to cuticle disorders.Commercial hybrids are often developed from crosses between flat and globe shaped parents because flat shape is thought to be dominant and fruit size intermediate.The objectives of this study were to determine the genetic basis of flat/globe fruit shape in large-fruited fresh-market tomato germplasm and to characterize its effects on several fruit traits.Twenty-three advanced single plant selections from the Fla.8000×Fla.8111B cross were selectively genotyped using a genome-wide SNP array,and inclusive composite interval mapping identified a single locus on the upper arm of chromosome 12 associated with shape,which we termed globe.A 238-plant F 2 population and 69 recombinant inbred lines for this region from the same parents delimited globe to approximately 392-kilobases.A germplasm survey representing materials from multiple breeding programs demonstrated that the locus explains the flat/globe shape broadly.A single base insertion in an exon of Solyc12g006860,a gene annotated as a brassinosteroid hydroxylase,segregated completely with shape in all populations tested.CRISPR/Cas9 knock out plants confirmed this gene as underlying the globe locus.In silico analysis of the mutant allele of GLOBE among 595 wild and domesticated accessions suggested that the allele arose very late in the domestication process.Fruit measurements in three genetic backgrounds evidenced that globe impacts fruit size and several fruit shape attributes,pedicel length/width,and susceptibility of fruit to weather check.The mutant allele of GLOBE appears mostly recessive for all traits except fruit size where it acts additively. | Edgar Sierra-Orozco Reza Shekasteband Eudald Illa-Berenguer Ashley Snouffer Esther van der Knaap Tong Geon Lee Samuel F.Hutton | 2021 | Horticulture Research2021，8，1： | 2 |
| 3 | Exploiting the diversity of tomato:the development of a phenotypically and genetically detailed germplasm collection显示文摘A collection of 163 accessions,including Solanum pimpinellifolium,Solanum lycopersicum var.cerasiforme and Solanum lycopersicum var.lycopersicum,was selected to represent the genetic and morphological variability of tomato at its centers of origin and domestication:Andean regions of Peru and Ecuador and Mesoamerica.The collection is enriched with S.lycopersicum var.cerasiforme from the Amazonian region that has not been analyzed previously nor used extensively.The collection has been morphologically characterized showing diversity for fruit,flower and vegetative traits.Their genomes were sequenced in the Varitome project and are publicly available(solgenomics.net/projects/varitome).The identified SNPs have been annotated with respect to their impact and a total number of 37,974 out of 19,364,146 SNPs have been described as high impact by the SnpEeff analysis.GWAS has shown associations for different traits,demonstrating the potential of this collection for this kind of analysis.We have not only identified known QTLs and genes,but also new regions associated with traits such as fruit color,number of flowers per inflorescence or inflorescence architecture.To speed up and facilitate the use of this information,F2 populations were constructed by crossing the whole collection with three different parents.This F2 collection is useful for testing SNPs identified by GWAs,selection sweeps or any other candidate gene.All data is available on Solanaceae Genomics Network and the accession and F2 seeds are freely available at COMAV and at TGRC genebanks.All these resources together make this collection a good candidate for genetic studies. | Estefanía Mata-Nicolás Javier Montero-Pau Esther Gimeno-Paez Víctor Garcia-Carpintero Peio Ziarsolo Naama Menda Lukas A.Mueller JoséBlanca Joaquín Cañizares Esther van der Knaap María JoséDíez | 2020 | Horticulture Research2020，7，1： | 2 |
| 4 | How does the corpus callosum mediate interhemispheric transfer? A review显示文摘 | Lisette J. van der Knaap Ineke J.M. van der Ham | 2011 | Behavioural Brain Research2011，，1： | 2 |
| 5 | A new class of tobacco chitinase homologous to bacterial exochitinases displays antifungal activity显示文摘 | Melchers L S Apothekerde Groot M van der Knaap J A | 1994 | Plant Journal1994，，5： | 1 |
| 6 | The spatial dispersion of economic activities and development trends in China: 1952-1985显示文摘 | Kim T J Knaap G J | 2001 | The Annals of Regional Science2001，35，1： | 1 |
| 7 | The second virial coefficients of the hydrogen iso- topes between 20 and 70 K显示文摘 | KNAAP H F P KNOESTER M KNOBLER C M | 1962 | Physica1962，28，1： | 1 |
| 8 | Measuring Urban Form: Is Portland Winning the War on Sprawl? 显示文摘 | Song Y G J Knaap | 2004 | Journal of the American Planning Association2004，70，： | 1 |
| 9 | Metabolic enzyme IMPDH is also a transcription factor regulated by cellularstate 显示文摘 | Kozhevnikova EN van der Knaap JA Pindyurin AV | 2012 | Cell2012，47，： | 1 |
| 10 | Quantitative analysis of urban form : a muhidisciplinary review 显示文摘 | Clifton K Ewing R Knaap G J | 2008 | Journal of Urbanism2008，1，1： | 1 |
| 11 | Managing urban growth with urban growth boundaries:A theoretical analysis显示文摘 | Ding R Knaap G Hopkins L | 1999 | Journal of Urban Economics1999，46，： | 1 |
| 12 | Fine mapping of fw3.2 controlling fruit weight in tomato显示文摘 | Na Zhang Marin Brewer Esther Knaap | 2012 | Theoretical and Applied Genetics2012，，2： | 1 |
| 13 | Do Plans Matter The Effect of Light Rail Plans on Lands Values in Station Area显示文摘 | Knaap G J Ding C Hopkins L D | 2004 | Joumal of Planning Education and Research2004，21，1： | 1 |
| 14 | Leukoencephalopathy with swelling in children and adolescents:MRI patterns and differential diagnosis显示文摘 | Van der Knaap MS Valk J Barth PG | 1995 | Neuroradiology1995，37，： | 1 |
| 15 | The price effects of an urban growth boundary in metropolitan Portland,Oregon显示文摘 | KNAAP G J | 1985 | Land Economics1985，61，1： | 1 |
| 16 | Mutagenic activity of acrylamide in eukaryotic systems but not in bacteria 显示文摘 | KNAAP A P KRANIERS C VOOGD W | 1988 | Mutagenesis1988，，3： | 1 |
| 17 | Deep water rice: a model plant to study stem elongation显示文摘 | Kende H Van der Knaap E Cho H-T | 1998 | Plant Physiology1998，118，： | 1 |
| 18 | New Urbanism and Housing Values: A Disaggregated Assessment显示文摘 | Song Y G J Knaap | 2003 | Journal of Urban Economics2003，54，2： | 1 |
| 19 | Talking smart in the United States,Working Paper,Prepared for the International Meeting on Multiple Intensive Land Use显示文摘 | Knaap Gerrit | 2002 | Sponsored by Habiform2002，，3： | 1 |
| 20 | Continuous dose-response relationship of the LDL-choles- terol-lowering effect of phytosterol intake显示文摘 | Isabelle D Rouyanne TR van der Knaap HCM | 2009 | J Nutr2009，139，2： | 1 |
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