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| 1 | Survivin isoforms and clinicopathological characteristics in colorectal adenocarcinomas using real-time qPCR显示文摘AIM:To investigate three isoforms of survivin in colorectal adenocarcinomas.METHODS:We used the LightCycler Technology(Roche),along with a common forward primer and reverse primers specific for the splice variants and two common hybridization probes labeled with fluorescein and LightCycler-Red fluorophore(LC-Red 640).Real time quantitative polymerase chain reaction(PCR) was performed on cDNAs from 52 tumor specimens from colorectal cancer patients and 10 unrelated normal colorectal tissues.In the patients group,carcinoembryonic antigen(CEA) and CA19-9 tumor markers were also measured immunochemically.RESULTS:Wild type survivin mRNA isoform was expressed in 48%of the 52 tumor samples,survivin-2b in 38%and survivin-ΔΕx3 in 29%,while no expression was found in normal tissues.The mRNA expression of wild type survivin presented a significant correlation with the expression of the ratio of survivin-2b,survivin-ΔΕx3,survivin-2b/wild type survivin and survivin-ΔΕx3/wild type survivin(P<0.001).The mRNA expression of wildsurvivin and survivin-ΔΕx3 was related with tumor size and invasion(P=0.006 and P<0.005,respectively).A significant difference was found between survivin-2b and morphologic cancer type.Also,the ratio of survivin-ΔEx3/ wild-survivin was significantly associated with prognosis.No association was observed between the three isoforms and grade,metastasis,Dukes stage and gender.The three isoforms were not correlated with CEA and CA19-9.CONCLUSION:Survivin isoforms may play a role in cell apoptosis and their quantification could provide information about clinical management of patients suffering from colorectal cancer. | Anastasia Pavlidou Maria Dalamaga Christos Kroupis George Konstantoudakis Maria Belimezi George Athanasas Kleanthi Dimas | 2011 | World Journal of Gastroenterology2011，17，12： | 9 |
| 2 | Association of survivin splice variants with prognosis and treatment of breast cancer显示文摘The purpose of this study was the overview of current knowledge regarding the use of survivin and its isoforms in prognosis and treatment of breast cancer. An advanced search of Medline was performed using the following search strategy: '(survivin isoforms) OR(survivin transcript variants) AND(breast cancer) AND(neoplasm OR tumor OR cancer OR carcinoma)'. Relevant studies were retrieved and processed thoroughly in order to analyze the related data. Besides wild-type survivin full-length transcript, another six splice variants have been identified. Overexpression of survivin and its isoforms leads to shorter overall and disease-free survival; the transcript variants are correlated with apoptosis and could assist prognosis prediction. It has been proved through numerous studies that inhibiting survivin isoforms might become a promising target of drug therapy of carcinomas. Use of small molecule YM155 could offer new therapy for triple negative breast cancer patients, while, chemotherapy with 5-fluorouracil + epirubicin + cyclophosphamide and Tax-Epi could be guided by survivin splice variants measurements. Survivin transcript variants could become prognostic biomarkers and could provide information about clinical managementof patients suffering from breast cancer. | Anastasia Pavlidou Christos Kroupis Kleanthi Dimas | 2014 | World Journal of Clinical Oncology2014，5，5： | 6 |
| 3 | Presence of high - risk human papillomavirus sequences in breast eancer tissues and association with histopathological characteristics 显示文摘 | Kroupis C Markou A Vourlidis N | 2006 | Clin Biochem2006，39，7： | 1 |
| 4 | Mutation scanning of exon 20 of the BRCA1 gene by high-resolution melting curve analysis显示文摘 | Vorkas PA Christopoulos K Kroupis C | | 0，，1： | 1 |
| 5 | Development and applications of a real - time quantitative RT - PCR method (QRT- PCR) for BRCA1 mRNA 显示文摘 | Kroupis C Stathopoulou A Zygalaki E | 2005 | Clinical Biochemistry2005，38，1： | 1 |
| 6 | Mutation scanning of exon 20 of the BRCAl gene by high-resolution melting curve analysis显示文摘 | Vorkas PA Christopoulos K Kroupis C | | 0，，1： | 1 |
| 7 | Physical training reduces peripheral markers of inflammation in patients with chronic heart failure显示文摘 | Adamopoulos S Parissis JT Kroupis C | 2001 | Eur Heart J2001，22，9： | 1 |
| 8 | Dimerization of thermomonospora fusca β-1,4-endoglucanase E2显示文摘 | MCGINNIS K KROUPIS C WILSON D B | 1993 | Biochemistry1993，32，32： | 1 |
| 9 | Antiinflammatory effects of cardiac resynchronization therapy in patients with chronic heart failure显示文摘 | Theodorakis GN Flevari P Kroupis C | 2006 | Pacing Clin Electrophysiol2006，29，3： | 1 |
| 10 | Cell adhesion molecules in relation to simvastatin and hormone replacement therapy in coronary artery disease显示文摘 | Sbarouni E Kroupis C Kyriakides Z S | 2000 | Eur Heart J2000，21，12： | 1 |
| 11 | Presence of high-risk human papillomavirus sequences in breast cancer tissues and association with histopathological characteristics显示文摘 | Kroupis C Markou A Vourlidis N | 2006 | Clin Biochem2006，39，7： | 1 |
| 12 | Antiinflammatory effects of cardiac resynchronization therapy in patients with chronic heart failure显示文摘 | Theodorakis GN Flevari P Kroupis C | 2006 | PACE2006，29，3： | 1 |
| 13 | Human papilloma virus(HPV)molecular diagnostics显示文摘 | Kroupis C Vourlid N | 2011 | Clin Chem Lab Med2011，49，11： | 1 |
| 14 | Physical training reduces peripheral markers of inflammation in patients with chronic heart failure显示文摘 | Adamopoulos S Parissis J Kroupis C | 2001 | Eur Heart J2001，22，9： | 1 |
| 15 | Cell adhesion molecules in relation to simvastatin and hormone replacement therapy in coronary artery disease显示文摘 | Sbarouni E Kroupis C Kyriakides ZS | 2000 | Eur Heart2000，21，12： | 1 |
| 16 | Presence of high-risk human papillomavirus sequences in breast cancer tissues and association with histopathological characteristics显示文摘 | Kroupis C Markou A Vourlidis N | | 0，，07： | 1 |
| 17 | Presence of high-risk human papillomavirus sequences in breast cancer tissues and association with histopathological characteristics 显示文摘 | Kroupis C Markou A Vourlidis N | 2006 | Clin Biochem2006，39，7： | 1 |
| 18 | Human papilloma virus (HPV) molecular diagnostics 显示文摘 | Kroupis C Vourlidis N | 2011 | Clin Chem Lab Med2011，49，11： | 1 |
| 19 | Presence of high-risk human papillomavirus sequences in breast cancer tissues and association with histopathological characteristics显示文摘 | Kroupis C Markou A Vourlidis N | | 0，，07： | 1 |
| 20 | Evaluation of carcinoembryonic antigen (CEA), epidermal growth factor receptor (EGFR), epithelial cell adhesion molecule EpCAM (GA733-2), and carbohydrate antigen 19-9 (CA 19-9) levels in colorectal cancer patients and correlation with clinicopathological characteristics显示文摘 | Mourtzikou A Stamouli M Kroupis C | 2012 | Clin Lab2012，58，56： | 1 |
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