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| 1 | Influence of puerarin,paeoniflorin,and menthol on structure and barrier function of tight junctions in MDCK and MDCK-MDR1 Cells显示文摘Objective:In this study,the influence of puerarin,paeoniflorin,and menthol on the structure and barrier function of tight junctions(TJs)in MadineDarby canine kidney epithelial(MDCK)and MDCK-multi-drug resistance 1(MDR1)cells was evaluated to determine the mechanisms by which the drugs cross the bloodebrain barrier(BBB).Method:Cells were treated with puerarin,paeoniflorin,and menthol followed by immunohistochemical staining with occludin,claudin-1,and F-actin.The cells were then observed using laser-scanning confocal microscopy.Average optical density(AOD)of the immunofluorescence images of the proteins were analyzed using ImageJ software while Transepithelial electrical resistance(TEER)was measured using an epithelial voltohmmeter.Results:Confocal microscopy revealed that puerarin-and paeoniflorin-treated tight junction proteins were conspicuous while menthol suppressed their expression.Correspondingly,AOD values of cells treated with puerarin or paeoniflorin,or both showed no difference compared to the control group(P>.05)while the menthol group value was downregulated.In 3 h,TEER of cells not treated with menthol were similar to the control group,while treatment with menthol significantly decreased TEER value(P<.05).In addition,application of menthol decreased TEER in MDCK cells earlier than in MDCK-MDR1 cells.Conclusion:Menthol but not puerarin and paeoniflorin may enhance paracellular transport and improve drug penetration of the BBB by disrupting the structure and,thereby,weakening the barrier function of TJs. | Lin Zhang Shouying Du Yang Lu Chang Liu Huichao Wu Bing Yang Jie Bai Pengyue Li | 2015 | Journal of Traditional Chinese Medical Sciences2015，2，2： | 4 |
| 2 | Synthesis and characterization of the flower-like La_(x)Ce_(1-x)O_(1.5+δ) catalyst for low-temperature oxidative coupling of methane显示文摘Lanthanum-based oxides are promising candidates for low-temperature oxidative coupling of methane(OCM).To further lower the OCM reaction temperature,the Ce doped flower-like La_(2)O_(2)CO_(3)microsphere catalysts were synthesized,achieving a significantly low reaction temperature (375℃) while maintaining high C_(2) hydrocarbon selectivity (43.0%).Doping Ce into the lattice of La_(2)O_(2)CO_(3)created more surface oxygen vacancies and bulk lattice defects,which was in favor of the transformation and migration of oxygen species at 350–400℃.The designed H_(2) temperature-programmed reduction (H_(2)-TPR) experiments provided strong evidence that the low reaction temperature of La_(x)Ce_(1-x)O_(1.5+δ)can be attributed to the transformation and migration of oxygen species,which dynamically generated surface oxygen vacancies for continuous oxygen activation to selectively convert methane.Moreover,designed temperatureprogrammed surface reaction (TPSR) clarified that two kinds of surface oxygen species in La_(x)Ce_(1-x)O_(1.5+δ)catalysts were concerned with catalytic performance,that is,the surface chemisorbed oxygen species for the activation of CH_(2)and the formation of CH_(2)·intermediates,surface La-Ce-O lattice oxygen species that caused the excessive oxidation of CH_(2)·intermediates.Finally,the factors affecting the transformation and migration of oxygen species were explored. | Ru Feng Pengyu Niu Bo Hou Qiang Wang Litao Jia Minggui Lin Debao Li | 2022 | Journal of Energy Chemistry2022，31，4： | 2 |
| 3 | LIM domain proteins Pinch1/2 regulate chondrogenesis and bone mass in mice显示文摘The LIM domain-containing proteins Pinch1/2 regulate integrin activation and cell–extracellular matrix interaction and adhesion.Here,we report that deleting Pinch1 in limb mesenchymal stem cells(MSCs)and Pinch2 globally(double knockout;dKO)in mice causes severe chondrodysplasia,while single mutant mice do not display marked defects.Pinch deletion decreases chondrocyte proliferation,accelerates cell differentiation and disrupts column formation.Pinch loss drastically reduces Smad2/3 protein expression in proliferative zone(PZ)chondrocytes and increases Runx2 and Col10a1 expression in both PZ and hypertrophic zone(HZ)chondrocytes.Pinch loss increases sclerostin and Rankl expression in HZ chondrocytes,reduces bone formation,and increases bone resorption,leading to low bone mass.In vitro studies revealed that Pinch1 and Smad2/3 colocalize in the nuclei of chondrocytes.Through its C-terminal region,Pinch1 interacts with Smad2/3 proteins.Pinch loss increases Smad2/3 ubiquitination and degradation in primary bone marrow stromal cells(BMSCs).Pinch loss reduces TGF-β-induced Smad2/3 phosphorylation and nuclear localization in primary BMSCs.Interestingly,compared to those from single mutant mice,BMSCs from dKO mice express dramatically lower protein levels ofβ-catenin and Yap1/Taz and display reduced osteogenic but increased adipogenic differentiation capacity.Finally,ablating Pinch1 in chondrocytes and Pinch2 globally causes severe osteopenia with subtle limb shortening.Collectively,our findings demonstrate critical roles for Pinch1/2 and a functional redundancy of both factors in the control of chondrogenesis and bone mass through distinct mechanisms. | Yiming Lei Xuekun Fu Pengyu Li Sixiong Lin Qinnan Yan Yumei Lai Xin Liu Yishu Wang Xiaochun Bai Chuanju Liu Di Chen Xuenong Zou Xu Cao Huiling Cao Guozhi Xiao | 2020 | Bone Research2020，8，4： | 2 |
| 4 | Wear resistance of a beating steel processed by laser surface remelting cooled by water 显示文摘 | Lin Pengyu Zhu Yongfu Zhou Hong | 2010 | ScriptaMaterialia2010，63，： | 1 |
| 5 | The mechanical properties and mierostructures of AZ91D magnesium alloy processed by selective laser- cladding with AI powder显示文摘 | LIN PENGYU ZHANG ZHIHUI REN LUQUAN | 2014 | Optics& Laser Technolo- gy2014，，60： | 1 |
| 6 | The mechanical properties and microstructures of AZ91D magnesium alloy processed by selective laser- cladding with A1 powder显示文摘 | LIN PENGYU ZHANG ZHIHUI REN LUQUAN | 2014 | Optics & Laser Technol- ogy2014，，60： | 1 |
| 7 | Wear resistance of a bearing steel processed by laser surface remelting cooled by water 显示文摘 | Lin Pengyu Zhu Yongfu Zhou Hong | 2010 | ScriptaMaterialia2010，63，： | 1 |
| 8 | Microstructure and rolling capability of modified AZ31–Ce–Gd alloys显示文摘 | Wenping Li Hong Zhou Pengyu Lin Shizhe Zhao | 2009 | Materials Characterization2009，，11： | 1 |
| 9 | Analysis of Food Safety Supervision and Spot-Check in Sichuan Province in 2019显示文摘Through the analysis on 211614 batches of samples from 35 categories of food safety supervision and inspection in Sichuan Province by Administration for Market Regulation of Sichuan Province in 2019,four suggestions were finally put forward.The analysis results showed that in 2019,the overall trend of food safety in Sichuan Province was stable and improved.A total of 30 categories of unqualified samples were detected,and a total of 12135 batches of unqualified samples were detected,with an average sample unqualified rate of 5.73%.Among them,the main problems of unqualified samples were as follows:the use of food additives beyond the scope and limit,accounting for 40.17%of the total number of unqualified samples,microbial contamination in food,accounting for 23.61%of the total unqualified samples,the residue index of agricultural and veterinary drugs in food not meeting the standard,accounting for 15.61%of the total unqualified samples,the quality index in food not meeting the standard,accounting for 8.51%of the total number of unqualified samples,and heavy metals and other pollutants in food exceeding the standard,accounting for 7.10%of the total unqualified samples. | Pengyu CHEN Wei WANG Xueli WU Lili JI Jiamin ZHANG Zhipin ZHAO Rui ZHANG Ting BAI Bo HOU Yixi YANG Lin CHEN | 2022 | Agricultural Biotechnology2022，11，2： | 0 |
| 10 | Facile access to C-N bonds via unexpected side reactions of Knoevenagel condensation and their application in high-efficiency organic solar cells显示文摘The construction of C-N bonds is of great importance in the fields of biology,medicine,chemistry and materials science.Here,the replacement of organic base from pyridine to piperidine in the Knoevenagel condensation process unexpectedly yields a series of novel organic molecules containing C-N bonds.Interestingly,the synthesis method does not require any external transition-metals catalysis,and photo-/electro-catalysis.Additionally,when the new compound 1b is added as a third component to a well-known binary system of PM6:Y6,the efficiency of the organic solar cell is significantly improved,resulting in an outstanding efficiency of 18.0%,which is one of the highest values reported to date for PM6:Y6-based ternary organic solar cells. | Daobin Yang Shuncheng Yang Hongqian Wang Lin Xie Pengyu Yan Ziyi Ge | 2024 | Science China Chemistry2024，67，1： | 0 |
| 11 | Composition,dispersion,and health risks of bioaerosols in wastewater treatment plants:A review显示文摘Bioaerosols are defined as airbome particles(0.05-100 um in size)of biological origin.They are considered potentially harmful to human health as they can contain pathogens such as bacteria,fungi,and viruses.This review summarizes the most recent research on the health risks of bioaerosols emitted from wastewater treatment plants(WWTPs)in order to improve the control of such bioaerosols.The concentration and size distribution of WWTP bioaerosols;their major emission sources,composition,and health risks;and considerations for future research are discussed.The major themes and findings in the literature are as follows:the major emission sources of WWTP bioaerosols include screen rooms,sludge-dewatering rooms,and acration tanks;the bioaerosol concentrations in screen and sludge-dewatering rooms are higher than those outdoors.WWTP bioacrosols contain a variety of potentially pathogenic bacteria,fungi,antibiotic resistance genes,viruses,endotoxins,and toxic metal(loid)s.These potentially,pathogenic substances spread with the bioaerosols,thereby posing health risks to workers and residents in and around the WWTP.Inhalation has been identified as the main exposure route,and children are at a higher risk of this than adults.Future studies should identify emerging contaminants,establish health risk assessments,and develop prevention and control systems. | Yunping Han Lin Li Ying Wang Jiawei Ma Pengyu Li Chao Han Junxin Liu | 2021 | Frontiers of Environmental Science & Engineering2021，15，3： | 0 |
| 12 | Study on Relationship Between Arc Behavior and Joint Forming in Cold Metal Transfer Welding Technology with Polarity⁃Exchanging显示文摘Cold metal transfer with polarity⁃exchanging is a new integrated welding technology based on MIG.Due to the alternation of the positive and negative polarities of the wire,favorable control upon the deposition rate and the welding shape coefficient was obtained in order to meet the desired joint design,and the related controlling principles and joint characteristics were reported.Droplet transfer physical behavior exhibited strong dependability on the studied welding parameters,such as welding voltage,welding current,wire feeding speed,and polarity⁃exchanging.This welding technology provides a new way for the welding of body⁃in⁃white(BIW)thin sheet with special demands.Moreover,the typical quality defects of MIG were greatly improved.Our study provides important technical information from the perspective of industrial application of MIG and sheds light on the higher application level of MIG in BIW welding. | Lijun Han Pengyu Lin Gengwei Zhang Lihui Zhong Changhua Liu | 2020 | Journal of Harbin Institute of Technology(New Series)2020，27，5： | 0 |
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