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| 1 | Theory and practice of cancer“Green Therapy”显示文摘Under the guidance of the theory of traditional Chinese medicine(TCM),the“Green Therapy”for tumors aims to“control”the condition,i.e.“When lethal treatments were adopted,they should be discontinued when six-tenths of the disease is eliminated;When treatments with moderate toxicity were chosen,they should be stopped when seven-tenths of the disease is eliminated;When treatments with mild toxicity were using,they should be discontinued when eight-tenths of the disease is eliminated;Even if using non-toxic treatments,they should be terminated when nine-tenths of the disease is wiped out..If the efficacy is below our expectation,we could repeat the treatments;But if go beyond the extent,healthy qi will be damaged and it will not be beneficial or even do harm to the repair capability of the body”,which underlines the end point of treatment,or“stop interventions when half of the disorder is gone”.Based on the Green Therapy theory,minimally invasive surgeries,TCM external treatments,natural medications and the biological target therapies are main approaches in our clinical cancer management.It differs from the modern treatment mode.The Green Therapy for cancers treats“patient’s quality of life”as our priority.This therapy system is particularly suitable for cancer patients who are aged and disqualified for or failed from classic anti-cancer treatments.This is a new trend of anti-cancer strategy characterized by effectiveness,minimally invasion,quick recovery,easy toleration and great sustainability. | Kaiwen Hu Tian Zhou | 2016 | Journal of Traditional Chinese Medical Sciences2016，3，4： | 8 |
| 2 | How do medical students understand disease behaviors?Evidence from event-related potentials显示文摘Objective: To investigate how medical students process Western medicine(WM) terms vs. traditional Chinese medicine(TCM) terms and how this is impacted by analogical priming from the perspective of cognitive neuroscience.Methods: The experiments were designed as a 2(TCM terms vs. WM terms) × 2(correct terms vs.incorrect terms) × 2(analogical priming task vs. non-priming task) scheme. A total of 26 medical students completed a non-priming judgment task and an analogical priming judgment task on medical terms. During the tasks, the participants were asked to make correct/incorrect judgments on WM terms and TCM terms, and their behavioral data and event-related potentials(ERPs) were recorded.Results: Behaviorally, the response speed and accuracy of WM terms were higher than those of TCM terms(both P <.001), indicating a prominent concreteness effect. Analogical priming shortened the response time to medical terms(P <.001), and the response time to TCM terms was shortened more significantly(P =.001). For ERPs, WM terms induced a larger P200(an early positive ERP component), a smaller N400(a negative ERP component), and a higher late positive ERP component, indicating superiority of attention adjustment, smaller-scale semantic activation, and a higher cost of late semantic analysis and integration. However, the analogical priming eliminated the difference between WM and TCM terms in P200 and N400 while maintaining it in the LPC. This suggests that WM terms are sensitive to analogical attention adjustment, and TCM terms are susceptible to analogical semantic integration.Conclusion: Our findings suggest that WM and TCM disease behavior terms may initially differ in concreteness or the use of a verbal-linguistic system. Analogy is more conducive to understanding TCM terms. This research provides important neuroscientific evidence of the difference in thinking between TCM and WM. | Huiwen Huang Buxin Han Chunhua Jia Cuilan Ma Jin Guo Sisi Ma | 2022 | Journal of Traditional Chinese Medical Sciences2022，9，2： | 7 |
| 3 | Transdermal permeation of Zanthoxylum bungeanum essential oil on TCM components with different lipophilicity显示文摘Objective:To investigate the percutaneous penetration enhancement effect of essential oil from Zanthoxylum bungeanum Maxim.(Z.bungeanum oil)on active components in externally-applied traditional Chinese medicines.Methods:Five model drugs,geniposide,puerarin,ferulic acid,tetramethylpyrazine,and osthole,were chosen based on their lipophilicity and tested using in vitro transdermal permeation studies consisting of Franz diffusion cells and full thickness rat abdominal skin.Scanning electron microscopy was employed to observe the morphological changes of rat skin tissue after treatment with Z.bungeanum oil.The molecular interactions between the oil and the polar head groups in stratum corneum(SC)lipids were monitored using molecular dynamic simulation,and the SC/vehicle partition coefficients and saturation solubilities of the selected model drugs treated with and without the oil were also determined to ascertain its mechanisms of action.Results:As oil concentration increased,the log ERflow trended toward a negative linear relationship with the lipophilicity of drugs.After treatment with Z.bungeanum oil,a mild lifting up and wrinkle on the SC surface were observed,and appeared to become more pronounced as oil concentration increased.There was no significant difference between the control and the Z.bungeanum oil at different concentrations in terms of saturation solubility of GP,while saturation solubilities of the 4 other drugs gradually increased as oil concentration increased.The oxygen-containing constituents in Z.bungeanum oil,such as terpinen-4-ol and 1,8-cineole,which accounted for 57.95%of total oil,could form stable hydrogen bonds with the polar head group of ceramide 3.Conclusion:Z.bungeanum oil facilitated transdermal permeation of drugs with different lipophilicity,including the extremely hydrophilic and lipophilic drugs,whereas it exhibited greater enhancement activity for strongly hydrophilic drugs.The mechanisms of transdermal permeation enhancement by the oil could be explained with SC/vehicle partition coefficient,saturation solubility,and the interactions with SC lipids. | Yi Lan Jingyan Wang Shuwang He Shuying Li Peng Tan Hongmei Lin Qing Wu | 2016 | Journal of Traditional Chinese Medical Sciences2016，3，3： | 6 |
| 4 | Effects of Xiangsha Liujunzi decoction drug serum on gastric antrum smooth muscle cells from rats with functional dyspepsia by regulating gastrointestinal hormones显示文摘Objective:To observe the effect and mechanism of Xiangsha Liujunzi decoction(XSLJZD)drug serum on gastric antrum smooth muscle cells(SMCs)in rats with functional dyspepsia(FD).Methods:Gastric antrum SMCs from rats with FD were isolated,cultured,and then divided into six groups as follows:control,model,domperidone,low-dose XSLJZD(LXSLJZD),medium-dose XSLJZD(MXSLJZD),and high-dose XSLJZD(HXSLJZD).Each group was administered the corresponding drug serum for intervention.Drug serum intervention conditions and proliferative activity of SMCs were tested by cholecystokinin octapeptide.Ghrelin,gastrin,somatostatin,and substance P(SP)levels were measured by ELISA.Somatostatin and SP mRNA expression was measured by real-time PCR.Results:A concentration of 10%drug serum for 24 h was decided to be the best intervention condition for later study.The mean optical density value in the model group was lower than that in the control group(P紏.001).Optical density values in the domperidone and HXSLJZD groups were higher than those in the model group(P?.025,P?.032,respectively).Gastrin,SP,and ghrelin levels in the model group were lower(P?.007,P?.037,P?.005,respectively),but somatostatin levels were higher,compared with those in the control group(P?.031).Gastrin,SP,and ghrelin levels in the domperidone,MXSLJZD,and HXSLJZD groups were higher than those in the model group(all P<.05).Somatostatin levels in the four drug-treated groups were lower than those in the model group(P?.002,P?.007,P?.001,P?.009,respectively).SP mRNA levels in the model group were lower than those in the control,domperidone,MXSLJZD,and HXSLJZD groups(P?.037 P?.016,P?.025,P?.002,respectively).Somatostatin mRNA levels in the model group were higher than those in the control and MXSLJZD groups(P紏.042,P紏.035).Conclusions:XSLJZD and domperidone drug serum effectively promote proliferative activity of gastric antrum SMCs in an FD model.The mechanism of this activity may be regulated by gastrointestinal hormones. | Ning Dai Jinghong Hu Yan Liu Dongyu Ge Ruijuan Dong Fengzhi Wu Jiaojiao Yu Mindan Chen Deborah CMa Feng Li | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，1： | 6 |
| 5 | Regulation of non-classical immune parameters in immune thrombocytopenic purpura mice by a spleen-invigorating,qi-replenishing and blood-containing formula显示文摘Objective:This study investigated the regulatory effect of non-classical immune parameters on immune thrombocytopenic purpura(ITP)mice by a spleen-invigorating,qi-replenishing and blood-containing formula(SQBF).Method:A total of 80 BALB/c mice were randomly divided into four equal groups(20 mice each):control group,model group,prednisone group and spleen-invigorating,qi-replenishing and blood-containing(SQBF)group.Mice in the model group,prednisone group,and SQBF group were administered anti-platelet serum to induce ITP.The dynamic variations of platelet counts in ITP mice were measured with an automatic blood analyzer before modeling and 48 h,and 8,12 and 15 days following APS injection.Levels of b-endorphin(b-EP),vasoactive intestinal peptide(VIP)and salivary IgA(SIgA)were detected by enzyme-linked immunosorbent assay(ELISA)on 15th day of experiment.Results:SQBF enhanced peripheral blood platelet counts in ITP mice similar to that of prednisone,and both groups showed a statistically significant response compared with the model group(P<.01).The SQBF significantly decreased b-EP levels compared with the model and prednisone intervention groups(P<.05),significantly increased the levels of VIP and SIgA in ITP mice compared with the model group(P<.05)and had significant protective effects on the thymus of ITP mice compared with the model group(P<.01).Conclusions:The SQBF had a similar effect to prednisone with regards to enhancing peripheral blood platelet counts in ITP mice.Furthermore,it decreased b-EP levels and increased VIP and SIgA,and protected the thymus.This shows that,on base of the brain-gut axis functions,some non-classical immune vascular active factors or neurotransmitters are also involved in immune responses,and also have relationship with the onset of ITP and bleeding and/or hemostasis.It needs further study to determine whether a change in these active factors is related to immediate hemostasis. | Tiantian Li Hao He Li Hou Yue Xu Xiaoyong Wu Yanping Sun Lei Zheng Zhixiong Chen Xinyi Chen Bei Qin | 2015 | Journal of Traditional Chinese Medical Sciences2015，2，2： | 6 |
| 6 | Xiaoyaosan improves depressive-like behaviors by regulating the NLRP3 signaling pathway in the rat cerebral cortex显示文摘Objective:To observe changes in the molecular expression of the NLR Family Pyrin Domain Containing Protein 3(NLRP3)pathway in depressed rats after treatment with Xiaoyaosan,and identify the regulatory mechanism of this compound.Methods:Male SpragueeD awley rats were randomly divided into five groups with 12 rats in each group,including the control group,model group,Fluoxetine group,Xiaoyaosan group,and MCC950 group.A depression model was generated by chronic immobilization stress(induced by 3 h of restraint immobilization every day),and the drugs were administered at the same time in each group for 21 days.The effects of Xiaoyaosan on behavioral changes of depressed rats were observed through macroscopic characterization,body mass,open field experiments,and a sucrose preference test.The m RNA and protein expression of the NLRP3 signaling pathway was examined by fluorescence real-time quantitative PCR and Western blot assays.Results:The Xiaoyaosan group,Fluoxetine group,and MCC950 group rats showed improved depressive behavior and an increased weight of sucrose water consumption.The protein and mRNA expression levels of NLRP3,Caspase-1,and IL-1 b were also decreased in the Fluoxetine,Xiaoyaosan,and MCC950 groups.Conclusion:NLRP3,Caspase-1,and IL-1 b protein and mRNA expression levels were increased in the cortex of depressed rats,while Xiaoyaosan protected cortical tissue in these rats by decreasing NLRP3,Caspase-1,and IL-1 b protein and mRNA expression. | Cong Chen Rong Yu Zhe Xue Zhiyi Yan Qinlai Bian Yajing Hou Yunzhi Chen Yueyun Liu Jiaxu Chen | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，3： | 5 |
| 7 | Classification of traditional Chinese medicine constitution based on facial features in color images显示文摘Objective:To explore the possible correlation between traditional Chinese medicine(TCM)constitution and facial features in color images and to improve the accuracy of automated constitution classification.Methods:Color images were taken of 5150 individuals of different professions.Automated face detection and key point positioning were performed on the collected images,which were then transformed into a standard size.The relationship between facial features and TCM constitution based on the red,green,blue(RGB)pixel and the local binary pattern(LBP)texture features was explored.Results:The overall accuracy rate and robustness of TCM constitution classification based on RGB features were low.Classification results of the phlegm-dampness,damp-heat,blood stasis,and balance constitutions achieved high accuracy rates.Classification accuracy rate using the LBP texture feature was higher than that of the RGB feature,with the best accuracy observed for the balance constitution.Conclusion:Application of computer image acquisition and processing of facial features may serve as an adjunct to the TCM diagnostic method of inspection. | Jian Zhang Shujuan Hou Ji Wang Lingru Li Pin Li Junwen Han Haiqiang Yao Ranran Sun Ziqing Li Zhen Lei Qi Wang | 2016 | Journal of Traditional Chinese Medical Sciences2016，3，3： | 5 |
| 8 | Color spaces of safflower (Carthamus tinctorius L.) for quality assessment显示文摘Objective:In this study,safflower(Carthamus tinctorius L.)was taken as a representative example to examine the application of color characteristics to evaluate quality.Methods:A computer vision system was established for the objective and nondestructive assessment of color using image processing algorithms.Color parameters were investigated based on the RGB,L*a*b and HSV color spaces.The content of hydroxysafflor yellow A(HSYA),a major bioactive constituent of safflower,was determined by high-performance liquid chromatography.The relationship between HSYA content and color values was investigated by Pearson correlation analysis.A multiple linear regression model was established to predict the HSYA content from color values.Results:The red color and lightness of safflower were found to be significantly related to HSYA content.The prediction equation obtained by multiple regression was reliable with an R2 value of 0.805(P<.01).Conclusion:The results suggest that the computer vision technique could be used as a promising and non-destructive technology for color measurement and quality evaluation of CHM. | Manfei Xu Chenzhao Du Na Zhang Xinyuan Shi Zhisheng Wu Yanjiang Qiao | 2016 | Journal of Traditional Chinese Medical Sciences2016，3，3： | 5 |
| 9 | Association between dietary habits and recurrent respiratory infection in children: A case-control study显示文摘Objective:To explore the association between dietary habits and recurrent respiratory infection(RRI)in children aged 0e14 years.Methods:This caseecontrol study compared dietary data of children with(cases)and without RRI(controls)collected via structured questionnaire.Participants were recruited from Chinese medicine clinics,hospitals,and children’s learning institutions in Beijing.A logistic regression analysis and odds ratio(OR)calculations were conducted using SPSS 17.0 software.Results:A total of 241 questionnaires were collected(case:control ratio:approximately 2:1).Frequent consumption of processed foods(OR Z 2.988,95%confidence intervals 1.375e6.491)and high-sugar foods(OR Z 2.268,95%confidence intervals 1.163e4.424),frequent picky eating(OR Z 2.614,95%confidence intervals 1.363e5.014),and a meat-heavy diet with fewer vegetables(OR Z 1.830,95%confidence intervals 1.358e2.467)correlated positively correlated with RRI.Additionally,57.80%of the children with RRI were addicted to high-sugar foods,compared with 41.57%of the children without RRI(P Z.015).Furthermore,63.16%of the children with RRI were picky eaters,compared with 48.31%of the children without RRI(P Z.024).Finally,30.92%of the children with RRI frequently consumed processed foods,compared with only 17.98%of the children without RRI(P Z.027Conclusion:Although RRI correlates positively with several dietary habits,in the future,prospective cohort studies with larger samples are needed to generalize these findings. | Wang Zhang He Yu Jinbang Shang Tiegang Liu Jiaju Ma Xiaohong Gu | 2015 | Journal of Traditional Chinese Medical Sciences2015，2，2： | 5 |
| 10 | Current anti-diabetes mechanisms and clinical trials using Morus alba L.显示文摘Backgrounds:Diabetes mellitus,especially type 2 diabetes,with its fast-rising prevalence,has become a global epidemic.Mulberry(Morus alba L.)leaf has been known to have hypoglycemic effects since ancient times.In Asia mulberry leaf is used as tea to complement the treatment of diabetes mellitus.The methods by which mulberry leaf affects the body and its mechanism when combined with chemical agents have been studied extensively.Conclusions:We summarize the possible mechanisms of the anti-diabetic effects of mulberry leaf based on extraction procedures,in vitro and in vivo experiments,and clinical trials.We also discuss the hypothesis that crosstalk and“critical nodes”may be useful for a deeper molecular understanding of the treatment and prevention of diabetes with mulberry leaf. | Simin Tian Mingmin Tang Baosheng Zhao | 2016 | Journal of Traditional Chinese Medical Sciences2016，3，1： | 4 |
| 11 | Effects of different acupuncture manipulations on protein expression in the parietal cortex of spontaneously hypertensive rats显示文摘Objective: To analyze the effects of twirling reinforcing and reducing manipulations on protein expression in parietal cortex of spontaneously hypertensive rats(SHRs), and elucidate the main mechanisms and differences between two manipulations in hypertension treatment.Methods: Rats were randomly divided into the control, model, twirling reinforcing manipulation(TRFM),and twirling reducing manipulation(TRDM) groups. The control and model groups received catch and fixation stimulations once a day for 14 days. The TRFM and TRDM groups were intervened once a day for 20 min for 14 days. On days 0, 2, 6, 10, and 14 after acupuncture, rat systolic blood pressures(SBPs) were measured. Differential protein(DP) expression in the rat parietal cortices was detected. Thereafter, GO functional significance and KEGG pathway enrichment analyses were performed.Results: Compared with the model group, SBP of rats in the TRDM and TRFM groups decreased on days 6 and 10 of acupuncture, respectively(P=.009;P <.001). Moreover, SBP of the TRDM group was significantly lower than that of the TRFM group on days 10 and 14 of acupuncture(P=.015;P=.013).Compared with control group, 601 and 1040 DPs were up-and downregulated, respectively, in the model group. Compared with model group, 44 and 28 up-and downregulated DPs were expressed, respectively,in the TRFM group. Compared with model group, expression of 616 and 427 up-and downregulated DPs,respectively, was found in the TRDM group. After combining the results of GO and KEGG enrichment analyses, five and one pathways were found to be related to the central antihypertensive mechanism of the parietal cortex during twirling reducing and reinforcing manipulations, respectively.Conclusion: TRDM showed a more effective antihypertensive effect on SHRs than TRFM;this antihypertensive effect was related to the regulation of different proteins and their biological functions. | Jiaojuan Wu Tianxiao Zeng Jingrong Liang Xudong Zhang Qi Xie Taotao Lv Pui Yee Wong Zhi Ji Qingguo Liu | 2021 | Journal of Traditional Chinese Medical Sciences2021，8，3： | 4 |
| 12 | Acu-TENS lowers blood lactate levels and enhances heart rate recovery after exercise显示文摘Objective:The primary aim of this study is to investigate the effect of application of transcutaneous electrical nerve stimulation over acupuncture points(Acu-TENS)on postexercise blood lactate level.The secondary aim is to explore the effect of Acu-TENS on heart rate recovery and its association with autonomic nervous system.Methods:Twenty healthy subjects(mean age 26.91.3)acting as their own controls,were randomized to receive either Acu-TENS or Placebo-TENS as the first of two intervention protocols,implemented one week apart.During Acu-TENS,subjects received 45 min TENS bilaterally over the acupoints Neiguan(PC6).Subjects receiving Placebo-TENS had identical electrode placement but with no electrical output from the TENS unit despite an active output light.Interventions were followed by a 10-min ergometer exercise at 70%age-predicted maximal heart rate.Oxygen consumption and heart rate(HR)were recorded continuously throughout exercise.Blood lactate and blood pressure were taken at 4 time points:prior to,immediately after,at 15-min after exercise,and when HR had returned to baseline values.Results:The post-exercise blood lactate level in the Acu-TENS group was lower than that of the placebo group by 1.120.39 mmol/L(p Z 0.01).The Acu-TENS group also had a faster return of HR to pre-exercise level compared to placebo(9.984.54 min,p Z 0.047).Heart rate variability analysis inferred reduced sympathetic modulation during exercise after Acu-TENS.There was no between-group difference in post-exercise oxygen consumption.Conclusion:Acu-TENS lowered post-exercise blood lactate level and enhanced heart rate recovery after moderate exercise.The role of Acu-TENS in exercise performance and energy metabolism warrants further investigation.(ClinicalTrails.gov Identifier:NCT01102634)a 2014 Beijing University of Chinese Medicine.Production and hosting by Elsevier B.V.This is an open access article under the CC BY-NC-ND license(http://creativecommons.org/licenses/by-nc-nd/3.0/). | Alice Y.M.Jones Shirley P.C.Ngai | 2014 | Journal of Traditional Chinese Medical Sciences2014，1，1： | 4 |
| 13 | Efficacy of Chinese herbal medicine in functional dyspepsia: A meta-analysis of randomized, double-blind, placebo-controlled trials显示文摘Objective:To evaluate the efficacy and safety of Chinese herbal medicine(CHM)for the treatment of functional dyspepsia(FD).Methods:Web of Science,PubMed,EMBASE,Cochrane Library,and four other Chinese electronic databases,including China National Knowledge Infrastructure(CNKI),Chinese Biological Medical Database(CBM),Chinese Scientific Journals Database(VIP),and WanFang Database were used to search(up to Feb,2016)for randomized,double-blind,placebocontrolled trials recruiting adults with FD treated with CHM.Study selection,data extraction,quality assessment,and data analyses were conducted based on Cochrane standards using Review Manager software.Results:Fourteen publications(1424 patients)were included.Evidence revealed that CHM was more efficacious than the placebo in improving global dyspepsia symptoms(RR,1.45;95%CI,1.31e1.60),Chinese medicine syndrome(CMS)(RR,1.36;95%CI,1.23e1.50),and quality of life(SMD,0.30;95%CI,0.15e0.45)in FD patients.Furthermore,the difference in the incidence of adverse events between CHM and placebo groups had no statistical significance(RR,1.06;95%CI,0.66e1.70).Conclusion:This meta-analysis demonstrates that CHM has a therapeutic potential in treating FD with a certain safety.However,due to the restricted number of trials included,wellplanned,long-term studies are necessary to provide credible evidence. | Yu Guo Jiajie Zhu Xiaolan Su Jianqin Yang Yijie Li Qingguo Wang Wei Wei | 2016 | Journal of Traditional Chinese Medical Sciences2016，3，3： | 4 |
| 14 | Influence of puerarin,paeoniflorin,and menthol on structure and barrier function of tight junctions in MDCK and MDCK-MDR1 Cells显示文摘Objective:In this study,the influence of puerarin,paeoniflorin,and menthol on the structure and barrier function of tight junctions(TJs)in MadineDarby canine kidney epithelial(MDCK)and MDCK-multi-drug resistance 1(MDR1)cells was evaluated to determine the mechanisms by which the drugs cross the bloodebrain barrier(BBB).Method:Cells were treated with puerarin,paeoniflorin,and menthol followed by immunohistochemical staining with occludin,claudin-1,and F-actin.The cells were then observed using laser-scanning confocal microscopy.Average optical density(AOD)of the immunofluorescence images of the proteins were analyzed using ImageJ software while Transepithelial electrical resistance(TEER)was measured using an epithelial voltohmmeter.Results:Confocal microscopy revealed that puerarin-and paeoniflorin-treated tight junction proteins were conspicuous while menthol suppressed their expression.Correspondingly,AOD values of cells treated with puerarin or paeoniflorin,or both showed no difference compared to the control group(P>.05)while the menthol group value was downregulated.In 3 h,TEER of cells not treated with menthol were similar to the control group,while treatment with menthol significantly decreased TEER value(P<.05).In addition,application of menthol decreased TEER in MDCK cells earlier than in MDCK-MDR1 cells.Conclusion:Menthol but not puerarin and paeoniflorin may enhance paracellular transport and improve drug penetration of the BBB by disrupting the structure and,thereby,weakening the barrier function of TJs. | Lin Zhang Shouying Du Yang Lu Chang Liu Huichao Wu Bing Yang Jie Bai Pengyue Li | 2015 | Journal of Traditional Chinese Medical Sciences2015，2，2： | 4 |
| 15 | Neuroprotective effect of Longshengzhi capsule following permanent middle cerebral artery occlusion in rats显示文摘Objective:Longshengzhi capsule(LSZC)is an optimized preparation based on the traditional Chinese Medicine formula Buyanghuanwu Decoction(BYHWD),and is approved by the China Food and Drug Administration for treating stroke-induced disability and vascular diseases.Herein,we examined the pharmacodynamics,anti-apoptotic and anti-oxidant actions,and potential mechanisms of action of LSZC following stroke in rats.Methods:Permanent middle cerebral artery occlusion(MCAO)was used as an ischemic stroke model.LSZC was administered intragastrically.We examined the survival rate,bodyweight,and neurological deficits in stroke rats.Brain infarct size was determined by 2,3,5-triphenyltetrazolium chloride staining.Brain pathology was examined using hematoxylin and eosin staining,Nissl staining,and TdT-mediated dUTP Nick-End Labeling staining.Malondialdehyde,catalase,superoxide dismutase,and glutathione levels were examined by commercial kits.Expression of Nrf2,heme oxygenase-1,Bax,Bcl-2,cleaved caspase-3,and caspase-3 proteins in brain tissue was measured by Western blot.Results:LSZC markedly improved the survival rate and bodyweight,and reduced infarct volume and neurological deficit scores,in MCAO stroke rats.LSZC also significantly attenuated oxidative stress,as indicated by decreased expression of malondialdehyde,and upregulation of Nrf2,heme oxygenase-1,catalase,superoxide dismutase,and glutathione.Moreover,LSZC significantly decreased apoptosis,including a decrease in Bax and cleaved caspase-3 expression,and an increase in Bcl-2,as well as a reduction in numbers of apoptotic neurons.Conclusion:LSZC treatment is neuroprotective against ischemic stroke,potentially via reducing oxidative stress and apoptosis.The Nrf2 and apoptotic signaling pathways may play important roles in the antioxidant and anti-apoptotic actions of LSZC. | Xiaoli Sun Shuzhen Guo Yong Wang Bangze Fu Junjie Liu Yili Zhang Ruocong Yang Chun Li Jian Gao Li Gao Wei Wang | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，1： | 4 |
| 16 | Systems pharmacology for traditional Chinese medicine with application to cardio-cerebrovascular diseases显示文摘Identified as a treasure of natural herbal products,traditional Chinese medicine(TCM)has attracted extensive attention for their moderate treatment effect and lower side effect.Cardio-cerebrovascular diseases(CCVD)are a leading cause of death.TCM is used in China to prevent and treat CCVD.However,the complexity of TCM poses challenges in understanding the mechanisms of herbs at a systems-level,thus hampering the modernization and globalization of TCM.A novel model,termed traditional Chinese medicine systems pharmacology(TCMSP)analysis platform,which relies on the theory of systems pharmacology and integrates absorption,distribution,metabolism,excretion and toxicity(ADME/T)evaluation,target prediction and network/pathway analysis,was proposed to address these problems.Here,we review the development of systems pharmacology,the TCMSP approach and its applications in the investigations of CCVD and compare it with other methods.TCMSP assists in uncovering the mechanisms of action of herbal formulas used in treating CCVD.It can also be applied in ascertaining the different syndrome patterns of coronary artery disease,decoding the multi-scale mechanisms of herbs,and in understanding the mechanisms of herbal synergism. | Yingxue Fu Yonghua Wang Boli Zhang | 2014 | Journal of Traditional Chinese Medical Sciences2014，1，2： | 4 |
| 17 | Percutaneous penetration enhancement effect of essential oil of mint (Mentha haplocalyx Briq.) on Chinese herbal components with different lipophilicity显示文摘Objective:To investigate the percutaneous penetration effect of essential oil of mint from Mentha haplocalyx Briq.on the complex active components in Chinese herbal external preparations,and assess its toxicity on the skin cells.Methods:The cytotoxicity of mint oil on HaCaT keratinocytes and CCC-ESF-1 fibroblasts was measured using an MTT assay.Five model drugs with a wide range of lipophilicity,namely osthole,tetramethylpyrazine,ferulic acid,puerarin,and geniposide,were tested using in vitro permeation studies to investigate the percutaneous penetration enhancement effect of mint oil.Secondary structure alterations of skin stratum corneum(SC)were measured using Fourier transform infrared spectroscopy(FTIR).Saturation solubilities and SC/vehicle partition coefficients of the five model drugs with and without mint oil were also determined to understand the potential mechanisms of the essential oil.Results:Half maximal inhibitory concentration(IC50)values of mint oil were significantly higher in HaCaT and CCC-ESF-1 cell lines than values in the well-established and standard penetration enhancer Azone.Conclusions:Mint oil at proper concentration could effectively facilitate percutaneous penetration of both lipophilic and hydrophilic drugs,and exhibit higher efficiency for moderate hydrophilic drugs.Mechanisms of penetration enhancement by mint oil could be explained with saturation solubility,SC/vehicle partition coefficient and the secondary structure change of SC. | Jingyan Wang Yi Lan Hui Li Yewen Zhang Qing Zhang Yanfeng Cao Qing Wu | 2014 | Journal of Traditional Chinese Medical Sciences2014，1，2： | 4 |
| 18 | A novel quality evaluation method for magnolia bark using electronic nose and colorimeter data with multiple statistical algorithms显示文摘Background:Magnolia bark(Magnolia Officinalis REHD.&WILS.and Magnolia officinalis REHD.&WILS.VAR.biloba REHD.&WILS,Hou Po in Chinese),is widely applied in clinical prescriptions and Chinese patent medicines.Origin place is a crucial factor affecting the quality of Hou Po,and chemical composition is an important index for evaluating its quality,which is closely related to its clinical efficacy.This study aims to develop a novel method for rapidly,accurately and comprehensively identifying the origin places of Hou Po and predicting the contents of its important chemical components.Methods:High performance liquid chromatography was used to analyze the contents of magnolol and honokiol and ultra-performance liquid chromatography the contents of magnocurarine and magnoflorine.The cold soak method was used to determine the contents of water-soluble extracts.The E-nose and colorimeter were used to determine the odor and color characteristics,respectively,of the collected Hou Po samples.Results:Using several statistical algorithms,different discriminant models based on the E-nose and colorimeter data were established to distinguish the origin place of Hou-Po and predict the chemical components of honokiol,magnolol,magnocurarine,magnoflorine and water-soluble extracts.The results showed that the Random Forest classifier combined with the ten-fold cross-validation method provided the highest classification accuracy for origin place,accounting for 99.53%among these models.The correlation coefficients between predicted and experimental values of the five chemical components were all higher than 0.96.Conclusion:This study has indicated that the electronic nose and colorimeter are promising methods for evaluating the quality of Chinese herbal medicines both qualitatively and quantitatively. | Jiahui Li Yuanyang Shao Yuebao Yao Yuetong Yu Guangzhao Cao Huiqin Zou Yonghong Yan | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，2： | 4 |
| 19 | Risk assessment models of integrative medicine indicators for malignant transformation of chronic atrophic gastritis:A registry study protocol显示文摘Objective:Traditional Chinese medicine(TCM)and modern medicine have both been used in arresting malignant transformation of chronic atrophic gastritis(CAG)in China with good therapeutic effect.However,no studies have been undertaken to assess the risk of malignant transformation in CAG patients using both modern medicine and TCM features.Our study aimed to develop risk assessment models for malignant transformation of CAG combining indicators of both TCM and modern medicine.These models will facilitate early warning and control of malignant transformation of CAG from the perspective of evidence-based integrative medicine.Methods:In the proposed registry study,a total of 1000 eligible CAG patients will be recruited from four hospitals in China.A 10-year follow-up study will be conducted both on-site and off-site to track the events of malignant transformation.Frequency analysis and chi-squared tests will be used to perform the comparative analysis on the prevalence of malignant transformation events and indicators in TCM or modern medicine in different groups.A multivariate Cox proportional hazard model will be used to perform correlation analyses of malignant transformation events and factors of TCM or modern medicine.Conclusion:The proposed study has been designed with a large sample size and long follow-up period,in which wide-ranging modern medicine and TCM indicators can be gathered over the whole process of malignant transformation of CAG.Based on this study,risk assessment models for malignant transformation ofCAGmaybe constructed fromthe perspective of integrative medicine.This may provide clinicians and patients with an optimized early warning system as well as prevention strategies for malignant transformation of CAG. | Yue Liu Wanjuan Wang Yuan Li Fusheng Liu Li Zhang Tao Tang Mingxuan Zang Yin Zhang Xia Ding | 2019 | Journal of Traditional Chinese Medical Sciences2019，6，3： | 3 |
| 20 | Potential mechanism of Huatan Qushi decoction on improving phlegm-dampness constitution using microRNA array and RT-qPCR targeting on hsa-miR-1237e3p显示文摘Background:Metabolic diseases pose considerable burden on the healthcare system worldwide,indicating the significance of prevention and treatment.In constitution theory of traditional Chinese medicine,phlegm-dampness constitution(PDC)is the common basis of metabolic diseases.In clinical practice,Huatan Qushi(HTQS)decoction targeting on PDC can effectively improve metabolic indicators.However,its underlying biochemical mechanism still remains unclear.Methods:Eight PDC participants received HTQS decoction for three months.Their blood was collected at baseline and 1 and 3 months after intervention started.Related biomedical indicators were detected.High-throughput sequencing and RT-qPCR were used for validation.Due to the missing data,repeated measures with missing values in mixed models were used.Results:After 3-month treatment,HDL-C level increased(P<.001)and FBG,FINS,and HbA1c all showed decreasing trend at different time points(all P<.05).After miRNA high-throughput sequencing,compared with the baseline,differential miRNAs at 1 and 3 months were screened,and target gene prediction and KEGG pathway enrichment analysis were performed.The results displayed that metabolic disease-related pathways mainly included pathways in cancer,PI3K-Akt signaling pathway,etc.Further,RT-qPCR showed that hsa-miR-1237e3p differed statistically(P=.008).Then we validated the target genes of hsa-miR-1237e3p in the“Pathways in Cancer”pathway including SDF1,AC,CRK,and HGF,also known as upstream target genes of PI3K/AKT pathway.The results showed that two indicators of CRK and HGF were in statistical significance(P=.045 and P=.036,respectively).Conclusion:PDC serves as a common basis for various metabolic diseases.Through adjusting PDC,HTQS decoction can improve biomedical indicators including blood glucose,HbA1c,insulin,and HDL-C.The target pathway is“Pathways in cancer”.Specifically,HTQS decoction acts on targets of CRK and HGF by regulating hsa-miR-1237e3p,and probably exerts effects on their downstream PI3K/AKT pathway. | Xiuping Zhang Ji Wang Baoxing Liu Haiqiang Yao Yu Chen Yuqing Yin Xuejing Yang Lingru Li | 2021 | Journal of Traditional Chinese Medical Sciences2021，8，1： | 3 |
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