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| 1 | Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus显示文摘Sparse labeling of neurons contributes to uncovering their morphology, and rapid expression of a fluorescent protein reduces the experiment range. To achieve the goal of rapid and sparse labeling of neurons in vivo, we established a rapid method for depicting the fine structure of neurons at 24 h post-infection based on a mutant viruslike particle of Semliki Forest virus. Approximately 0.014 fluorescent focus-forming units of the mutant virus-like particle transferred enhanced green fluorescent protein into neurons in vivo, and its affinity for neurons in vivo was stronger than for neurons in vitro and BHK21(baby hamster kidney) cells. Collectively, the mutant virus-likeparticle provides a robust and convenient way to reveal the fine structure of neurons and is expected to be a helper virus for combining with other tools to determine their connectivity. Our work adds a new tool to the approaches for rapid and sparse labeling of neurons in vivo. | Fan Jia Xutao Zhu Pei Lv Liang Hu Qing Liu Sen Jin Fuqiang Xu | 2019 | Neuroscience Bulletin2019，35，3： | 3 |
| 2 | The spontaneous emission of an excited atom embedded in photonic crystals with two atomic position-dependent bands显示文摘The spontaneous emission of an excited atom embedded in photonic crystals with two atomic position-dependent bands is investigated.The distribution of the density of states between two bands depends on the atomic position in a unit cell of the photonic crystal and is described with an atomic position-dependent parameter.The result shows that the emitted field and the time evolution of the upper-level population are affected by the atomic position and the gap width.The spontaneous emission spectrum in free space can be shifted and narrowed with the photonic reservoir and the gap width. | HUANG XianShan LIU HaiLian WANG Dong MO XuTao LIU HouTong | 2013 | Science China Chemistry2013，56，3： | 2 |
| 3 | Rabies Virus Pseudotyped with CVS-N2C Glycoprotein as a Powerful Tool for Retrograde Neuronal Network Tracing显示文摘Efficient viral vectors for mapping and manipulating long-projection neuronal circuits are crucial in structural and functional studies of the brain. The SAD strain rabies virus with the glycoprotein gene deleted pseudotyped with the N2 C glycoprotein(SAD-RV(DG)-N2 C(G)) shows strong neuro-tropism in cell culture, but its in vivo efficiency for retrograde gene transduction and neuro-tropism have not been systematically characterized.We compared these features in different mouse brain regions for SAD-RV-N2 C(G) and two other widely-used retrograde tracers, SAD-RV(DG)-B19(G) and r AAV2-retro. We found that SAD-RV(DG)-N2 C(G) enhanced the infection efficiency of long-projecting neurons by^10 times but with very similar neuro-tropism, compared with SAD-RV(DG)-B19(G). On the other hand, SAD-RV(DG)-N2 C(G) had an infection efficiency comparable with r AAV2-retro, but a more restricted diffusion range, and broader tropism to different types and regions of longprojecting neuronal populations. These results demonstrate that SAD-RV(DG)-N2 C(G) can serve as an effective retrograde vector for studying neuronal circuits. | Xutao Zhu Kunzhang Lin Qing Liu Xinpei Yue Huijie Mi Xiaoping Huang Xiaobin He Ruiqi Wu Danhao Zheng Dong Wei Liangliang Jia Weilin Wang Anne Manyande Jie Wang Zhijian Zhang Fuqiang Xu | 2020 | Neuroscience Bulletin2020，36，3： | 2 |
| 4 | WoT/SDN:Web of Things Architecture Using SDN显示文摘Wo T(Web of Things) integrates smart devices into Web by reusing and extending Web standards. While Web technology makes the developers' job easier,it faces security,management and efficiency challenges. We propose Wo T/SDN,the architecture of resource oriented Wo T built on SDN(Software Defined Network),in which applications could be developed through resource subscription and Mashup with the programmability provided by SDN. The key components are designed,including Security and Management Controller(SMC),various atomic services and resource subscription syntax. Three applications covering device management,data access and security protection are demonstrated. Compared to traditional resource-oriented Wo T systems,our test results show that SDN,with its logically centralized control capability and awareness of flow forwarding,provides new opportunity to improve performance,simplify management and enhance security for Wo T. | QIU Xiaofeng LIU Wenmao GAO Teng HE Xinxin WEN Xutao CHEN Pengcheng | 2015 | China Communications2015，12，11： | 2 |
| 5 | STX5 Inhibits Hepatocellular Carcinoma Adhesion and Promotes Metastasis by Regulating the PI3K/mTOR Pathway显示文摘Background and Aims:Syntaxin 5(STX5)is a member of the syntaxin or target-soluble SNAP receptor(t-SNARE)fam-ily and plays a critical role in autophagy.However,its function and molecular mechanism in tumor cell migration are still un-known.The role of STX5 in influencing hepatocellular carci-noma(HCC)is an important topic in our research.Methods:By using quantitative reverse transcription polymerase chain reaction(qPCR),western blotting,and immunohistochemical analysis of RNA and protein in tissues,we comprehensively evaluated data sets from public databases and clinical patient cohorts for STX5.The correlation of STX5 expression with the clinicopathological characteristics of HCC patients were assessed.In addition,we predicted signal pathways from dif-ferentially expressed genes(DEGs)and the Cancer Genome Atlas(TCGA)databases,and confirmed the prediction using integrated transcriptome and RNA-seq.We further investi-gated the underlying mechanisms of STX5 in the migration and adhesion of HCC cells both in vitro and in vivo.Results:In the TCGA dataset and our patient cohort,STX5 levels were significantly higher in HCC tissues than in adjacent normal liver tissues.At the same time,high expression of STX5 pre-dicted worse prognosis in patients with liver cancer.High ex-pression of STX5 indicates the decrease of adhesion and the increase of migration of HCC cells,and the conversion of epi-thelial-mesenchymal transition(EMT)in vitro via PI3K/mTOR pathway activation.Conversely,when Sirolimus,a phospho-inositide 3-kinase(PI3K)/AKT/mechanistic target of rapa-mycin(mTOR)inhibitor acts on cells simultaneously,STX5 overexpression-mediated enhancement of HCC metastasis is reversed.Double-negative regulation of STX5 and mTOR further enhanced the inhibitory effect of STX5 on HCC me-tastasis.In vivo,STX5 knockdown inhibited the metastasis of HCC cells.Conclusions:Our study demonstrates a novel research result that STX5 promotes HCC metastasis through PI3K/mTOR pathway.We believe that combined inhibition of STX5 and mTOR is a potential treatment for effectively pro-longing patient survival and inhibiting HCC metastasis. | Bin Zhang Ziyin Zhao Youpeng Wang Tingting Guo Mingyang He Ge Guan Pai Peng Jinzhen Cai Bingyuan Zhang Xutao Liu Qiaoling Song | 2023 | Journal of Clinical and Translational Hepatology2023，11，3： | 1 |
| 6 | Visible-Light-Sensitive SrCO3/AgI Hybrids for Tetracycline Degradation显示文摘The SrCO3/AgI photocatalysts were prepared via a co-precipitation method by using SrCO3 as a co-photocatalyst and AgI as a photo sensitizer.X-ray diffraction,field emission scanning electron microscope,X-ray photoelectron spectrometer,UV-vis diffuse reflectance spectroscopy and electrochemical impedance spectroscope were used to analyze the structure,micro-morphology,chemical compositions,optical properties and photo-generated carrier behaviors of the as-prepared samples,respectively.The photocatalytic degradation mechanism of the as-developed composites was also proposed.Analysis results show SrCO3,an insulator,can improve the photocatalytic performances and recyclability of AgI for degrading tetracycline under visible light.As the theoretical molar ratio of Sr(NO3)2 to AgNO3 increases,the degradation efficiency of the hybrids first increases and then descends.When the theoretical molar ratio of that is 1:1,it acquires the maximum of 66.6% within 8 min.This is higher than 32.0% of pure AgI and 34.0% of SrCO3.Moreover,after three times degradations it is 63.0%,which is higher than 13.6% of AgI.The improvement of the photocatalytic performance of the sample is attributed to the construction of hybrids.The main activated species in catalysis process are superoxide radicals. | 贾云宁 吴湘锋 LI Hui ZHANG Weiguang WANG Hui CHANG Tianlong FU Yunxuan LIU Xutao GUO Yudong SHANG Jialu | 2020 | Journal of Wuhan University of Technology(Materials Science)2020，35，5： | 1 |
| 7 | ING5 overexpression upregulates miR-34c-5p/Snail1 to inhibit EMT and invasion of lung cancer cells显示文摘ING5 belongs to the inhibitor of growth(ING)candidate tumor suppressor family,which is involved in multiple cellular functions,such as cell cycle regulation,apoptosis,and chromatin remodelling.Previously,we reported that ING5 overexpression inhibits EMT by regulating EMT-related molecules,including Snail1,at the mRNA and protein levels.However,the mechanisms remain unclear.In the current study,we identify that ING5 overexpression induces the upregulation of miR-34c-5p.The expression levels of both ING5 and miR-34c-5p in NSCLC tissues from the TCGA database are decreased compared with that in adjacent tissues.Higher expression levels of both ING5 and miR-34c-5p predict longer overall survival(OS).Snail1 is the target gene of miR-34c-5p,as predicted by an online database,which is further verified by a dual-luciferase reporter assay.The expression level of Snail1 in NSCLC cells is markedly reduced following miR-34c-5p overexpression,leading to the inactivation of the Snail1 downstream TGF-β/Smad3 signaling pathway.The TGF-βsignaling-specific inhibitor LY2157299 reverses the enhanced EMT,proliferation,migration,and invasion abilities induced by the miR-34c-5p inhibitor.Furthermore,tail vein injection of miR-34c-5p agomir inhibits xenografted tumor metastasis.Overall,this study concludes that miR-34c-5p,induced by ING5 overexpression,is a tumor suppressor that targets Snail1 and mediates the inhibitory effects of ING5 on the EMT and invasion of NSCLC cells.These results provide a novel mechanism mediating the antitumor effects of ING5. | Jiong Yang Xinli Liu Yang Sun Xutao Zhang Yong Zhao Haihua Zhang Qibing Mei Jin Meng Feng Zhang Tao Zhang | 2023 | Acta Biochimica et Biophysica Sinica2023，55，5： | 1 |
| 8 | Etendue analysis and measurement of light source with elliptical reflector 显示文摘 | Sun Xutao Zheng Zhenrong Liu Xu | 2006 | Displays2006，27，2： | 1 |
| 9 | Etendue analysis and measurement of light source with elliptical reflector 显示文摘 | Xutao Sun Zhenrong Zheng Xu Liu Peifu GU | | Displays0，27，2： | 1 |
| 10 | GRB 220408B:A Three-episode Burst from a Precessing Jet显示文摘Jet precession has previously been proposed to explain the apparently repeating features in the light curves of a few gamma-ray bursts(GRBs).In this paper,we further apply the precession model to a bright GRB 220408B by examining both its temporal and spectral consistency with the predictions of the model.As one of the recently confirmed GRBs observed by our GRID CubeSat mission,GRB 220408B is noteworthy as it exhibits three apparently similar emission episodes.Furthermore,the similarities are reinforced by their strong temporal correlations and similar features in terms of spectral evolution and spectral lags.Our analysis demonstrates that these features can be well explained by the modulated emission of a Fast-Rise-Exponential-Decay(FRED)shape light curve intrinsically produced by a precessing jet with a precession period of 18.4_(-0.2)~(+0.2)s,a nutation period of11.1_(-0.2)~(+0.2)s and viewed off-axis.This study provides a straightforward explanation for the complex yet similar multiepisode GRB light curves. | Zijian Zhang Yi-Han Iris Yin Chenyu Wang Xiangyu Ivy Wang Jun Yang Yan-Zhi Meng Zi-Ke Liu Guo-Yin Chen Xiaoping Fu Huaizhong Gao Sihao Li Yihui Liu Xiangyun Long Yong-Chang Ma Xiaofan Pan Yuanze Sun Wei Wu Zirui Yang Zhizhen Ye Xiaoyu Yu Shuheng Zhao Xutao Zheng Tao Zhou Qing-Wen Tang Qiurong Yan Rong Zhou Zhonghai Wang Hua Feng Ming Zeng Bin-Bin Zhang | 2023 | Research in Astronomy and Astrophysics2023，23，11： | 0 |
| 11 | In-situ Synthesis of SnO2 Quantum Dots/ZnS Nanosheets Heterojunction as a Visible-light-driven Photocatalyst for Degradation of Rhodamine B,Potassium Dichromate and Tetracycline显示文摘The SnO2 quantum dots(SnO2QDs)/ZnS nanosheets(ZnSNs)heterojunction was fabricated via an in-situ synthetic method at room temperature.Rhodamine B,potassium dichromate,and tetracycline were used to discuss the photocatalytic activities of the as-prepared samples under the visible light illumination.The photocatalytic mechanism of the as-prepared samples was also proposed.The experimental results indicate that the degradation efficiency of the as-prepared SnO2QDs/ZnSNs heterojunction first increases and then decreases with increasing the usage of ZnSNs.When the mass ratio of SnO2QDs to ZnSNs is 1:2 in 180 min,the as-prepared samples have the highest degradation efficiency of 89.1%for rhodamine B,97.7%for potassium dichromate,and 83.8%for tetracycline,which are much higher than 51.7%,26.8%,and 0.9%of pure SnO2QDs as well as 37.9%,87.1%,and 19.1%of pure ZnSNs,respectively.After it is repeatedly degraded for 3 times,it possesses the degradation efficiency of 62.5%for rhodamine B,which increases by 200.5%in comparison with 20.8%of the pure SnO2QDs.Moreover,the enhanced photocatalytic performances of the as-prepared hybrids are attributed to the formation of heterojunction between the SnO2QDs and ZnSNs.In addition,hydroxyl radicals and superoxide anion radicals play major roles during the photocatalytic degradation process,while holes play a minor role. | 张琛旭 吴湘锋 JIA Yunning CHANG Tianlong LIU Zhifeng FU Yunxuan LIU Xutao 王惠 SHI Yimai ZUO Yuqian | 2020 | Journal of Wuhan University of Technology(Materials Science)2020，35，4： | 0 |
| 12 | Numerical Study on Flow-Induced Vibration of Ejector Structure显示文摘High-speed airflow in wind tunnel tests usually causes dramatic vibration of ejector structure,which may lead to fatigue and even destruction of the wind tunnel.Therefore,analyzing and solving the flow-induced vibration problem is a tough and indispensable part of the wind tunnel security design.In this paper,taking a kind of two-stage ejector as the study object,multiple numerical simulation methods are adopted in order to carry out research on the analysis technique of the flow-induced vibration characteristics of ejector structure.Firstly,the structural dynamics characteristic is analyzed by using the ejector structural dynamics numerical model,which is built on the basis of finite element method.Secondly,the complex flow phenomenon is explored applying numerical fluid-dynamics model of the inner flow field of the ejector,which is constructed on the basis of finite volume method.Finally,based on the two numerical models above,the vibration response of the ejector structure induced by the high-speed airflow is computed via the fluid-solid coupling technique.The comparison of the simulation results with the actual vibration test indicates that these numerical simulation methods can accurately figure out the rule of flow-induced vibration of ejectors. | NIE Xutao LIU Zongzheng QIN Chaojin ZHANG Wei | 2019 | Transactions of Nanjing University of Aeronautics and Astronautics2019，36，5： | 0 |
| 13 | Polarized Water Driven Dynamic PN Junction-Based Direct-Current Generator显示文摘There is a rising prospective in harvesting energy from the environment,as in situ energy is required for the distributed sensors in the interconnected information society,among which the water flow energy is the most potential candidate as a clean and abundant mechanical source.However,for microscale and unordered movement of water,achieving a sustainable direct-current generating device with high output to drive the load element is still challenging,which requires for further exploration.Herein,we propose a dynamic PN water junction generator with moving water sandwiched between two semiconductors,which outputs a sustainable direct-current voltage of 0.3 V and a current of 0.64μA.The mechanism can be attributed to the dynamic polarization process of water as moving dielectric medium in the dynamic PN water junction,under the Fermi level difference of two semiconductors.We further demonstrate an encapsulated portable power-generating device with simple structure and continuous direct-current voltage output of 0.11 V,which exhibits its promising potential application in the field of wearable devices and the IoTs. | Yanghua Lu Yanfei Yan Xutao Yu Xu Zhou Sirui Feng Chi Xu Haonan Zheng Zunshan Yang Linjun Li Kaihui Liu Shisheng Lin | 2021 | Research2021，，1： | 0 |
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