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| 1 | Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase显示文摘包含 methyltransferase 建筑群的象 3 一样的 methyltransferase (METTL3 ) 催化 N6-methyladenosine (m6A ) 形成,一个新奇 epitranscriptomic 标记;然而,这建筑群的性质仍然保持大部分未知。这里,我们报导人的 m6A methyltransferase 建筑群, Wilm 的肿瘤 1 伙伴蛋白质(WTAP ) 和象 14 一样的 methyltransferase (METTL14 ) 的二个新部件。WTAP 与 METTL3 和 METTL14 交往,并且为他们的本地化被要求进在 vivo 与处理因素的 pre-mRNA 并且为 m6A methyltransferase 的催化活动充实的原子点缀。RNA 的多数在 vivo 由 WTAP 和 METTL3 跳了代表包含一致 m6A 主题的 mRNAs。当 WTAP 不在时, METTL3 的 RNA 有约束力的能力强烈被减少,建议 WTAP 可以工作调整到 mRNA 目标的 m6A methyltransferase 建筑群的招募。而且,在有 photoactivatable-ribonucleoside-enhanced crosslinking 和 immunoprecipitation (同等片断) 的联合的 transcriptomic 分析说明那 WTAP 和 METTL3 调整涉及抄写并且 RNA 处理的基因拼接的表示和选择。在 zebrafish 胚胎的调停 Morpholino 的击倒的指向 WTAP 或 METTL3 引起了织物区别缺点并且增加了 apoptosis。这些调查结果提供 WTAP 可以在 m6A methyltransferase 建筑群作为一个规章的子单元工作并且在 RNA 的 epitranscriptomic 规定起一个关键作用的充分证据新陈代谢。 | Xiao-Li Ping Bao-Fa Sun Lu Wang Wen Xiao Xin Yang Wen-Jia Wang Samir Adhikari Yue Shi Ying Lv Yu-Sheng Chen Xu Zhao Ang Li Ying Yang Ujwal Dahal Xiao-Min Lou Xi Liu Jun Huang Wei-Ping Yuan Xiao-Fan Zhu Tao Cheng Yong-Liang Zhao Xinquan Wang Jannie M Rendtlew Danielsen Feng Liu Yun-Gui Yang | 2014 | Cell Research2014，24，2： | 244 |
| 2 | Fast increasing of surface ozone concentrations in Pearl River Delta characterized by a regional air quality monitoring network during 2006–2011显示文摘Based on the observation by a Regional Air Quality Monitoring Network including 16 monitoring stations, temporal and spatial variations of ozone(O3), NO2and total oxidant(Ox) were analyzed by both linear regression and cluster analysis. A fast increase of regional O3concentrations of 0.86 ppbV/yr was found for the annual averaged values from 2006 to 2011 in Guangdong, China. Such fast O3increase is accompanied by a correspondingly fast NOx reduction as indicated by a fast NO2 reduction rate of 0.61 ppbV/yr. Based on a cluster analysis, the monitoring stations were classified into two major categories – rural stations(non-urban) and suburban/urban stations. The O3concentrations at rural stations were relatively conserved while those at suburban/urban stations showed a fast increase rate of 2.0 ppbV/yr accompanied by a NO2 reduction rate of 1.2 ppbV/yr. Moreover, a rapid increase of the averaged O3 concentrations in springtime(13%/yr referred to 2006 level) was observed, which may result from the increase of solar duration, reduction of precipitation in Guangdong and transport from Eastern Central China. Application of smog production algorithm showed that the photochemical O3production is mainly volatile organic compounds(VOC)-controlled. However, the photochemical O3production is sensitive to both NOx and VOC for O3pollution episode. Accordingly, it is expected that a combined NOx and VOC reduction will be helpful for the reduction of the O3 pollution episodes in Pearl River Delta while stringent VOC emission control is in general required for the regional O3 pollution control. | Jinfeng Li Keding Lu Wei Lv Jun Li Liuju Zhong Yubo Ou Duohong Chen Xin Huang Yuanhang Zhang | 2014 | Journal of Environmental Sciences2014，26，1： | 51 |
| 3 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 4 | Consensus on the application of negative pressure wound therapy of diabetic foot wounds显示文摘Because China is becoming an aging society,the incidence of diabetes and diabetic foot have been increasing.Diabetic foot has become one of the main health-related killers due to its high disability and mortality rates.Negative pressure wound therapy(NPWT)is one of the most effective techniques for the treatment of diabetic foot wounds and great progress,both in terms of research and its clinical application,has been made in the last 20 years of its development.However,due to the complex pathogenesis and management of diabetic foot,irregular application of NPWT often leads to complications,such as infection,bleeding and necrosis,that seriously affect its treatment outcomes.In 2020,under the leadership of Burns,Trauma and Tissue Repair Committee of the Cross-Straits Medicine Exchange Association,the writing group for‘Consensus on the application of negative pressure wound therapy of diabetic foot wounds’was established with the participation of scholars from the specialized areas of burns,endocrinology,vascular surgery,orthopedics and wound repair.Drawing on evidence-based practice suggested by the latest clinical research,this consensus proposes the best clinical practice guidelines for the application and prognostic evaluation of NPWT for diabetic foot.The consensus aims to support the formation of standardized treatment schemes that clinicians can refer to when treating cases of diabetic foot. | Shizhao Ji Xiaobin Liu Jie Huang Junmin Bao Zhaohong Chen Chunmao Han Daifeng Hao Jingsong Hong Dahai Hu Yufeng Jiang Shang Ju Hongye Li Zongyu Li Guangping Liang Yan Liu Gaoxing Luo Guozhong Lv Xingwu Ran Zhongmin Shi Juyu Tang Aiping Wang Guangyi Wang Jiangning Wang Xin Wang Bing Wen Jun Wu Hailin Xu Maojin Xu Xiaofei Ye Liangxi Yuan Yi Zhang Shichu Xiao Zhaofan Xia | 2021 | Burns & Trauma2021，9，1： | 17 |
| 5 | OsVPS9A Functions Cooperatively with OsRAB5A to Regulate Post-Golgi Dense Vesicle-Mediated Storage Protein Trafficking to the Protein Storage Vacuole in Rice Endosperm Cells显示文摘在米饭内乳房间, glutelins 作为 proglutelins 在不平的 endoplasmic 蜂窝胃上被综合并且被排序到称为蛋白质身体 II (PBII ) 的 theprotein 存储液泡(PSV ) ,在他们被变换成成熟形式的地方。稠密的泡(DV ) 调停了在大米种子的 proglutelins 的 trafficking 被建议了,但是这个过程的 post-Golgi 控制是 largelyunknown。DV 是否能与 PSV 直接熔化,是争论的另一件事。在这研究,我们建议规章的 mechanismunderlying 调停 DV, post-Golgi proglutelin trafficking 到 PBII (PSV ) 。gpa2, OsVPS9A 的 loss-of-function 异种,编码 OsRAB5A 的 GEF,积累了 uncleaved proglutelins。Proglutelins 对到沿着在 DV 形式的房间墙的 apoplast 的 paramural bodiesand 指向 mis,它在 PBII 尺寸导致了伴随物减小。Previouslyreported gpa1,在 OsRab5a 变异,有类似的显型,当时两倍异种加重了的 gpa1gpa2 条件。另外, OsVPS9A 在 vitro 并且在 vivo 与 OsRAB5A 交往了。我们断定 OsVPS9A 和 OsRAB5A 可以一起工作并且在调停 DV 的 post-Golgi proglutelintrafficking 起一个规章的作用到 PBII (PSV ) 。DV 可能直接熔化到 PBII (PSV ) 交付货物的证据也是 presented.Key 词: | Feng Liu Yulong Ren Yihua Wang Cheng Peng Kunneng Zhou Jia Lv Xiuping Guo Xin Zhang Jianmin wan Yiqun Bao | 2013 | Molecular Plant2013，6，6： | 16 |
| 6 | Evaluation of liver functional reserve by combining D-sorbitol clearance rate and CT measured liver volume显示文摘AIM: Our research attempted to evaluate the overall functional reserve of cirrhotic liver by combination of hepatic functional blood flow, liver volume, and ChildPugh′s classification, and to discuss its value of clinical application.METHODS: Ninety two patients with portal hypertension due to hepatic cirrhosis were investigated. All had a historyof haematemesis and hematochezia, esophageal and gastric fundus varices, splenomegaly and hypersplenia.A 2-year follow-up was routinely performed and no one was lost. Twenty two healthy volunteers were used as control group. Blood and urine samples were collected 4times before and after intravenous D-sorbitol infusion.The hepatic clearance (CLH) of D-sorbitol was then calculated according to enzymatic spectrophotometric method while the total blood flow (QToTAL) and intrahepatic shunt (RINs) were detected by multicolor Doppler ultrasound, and the liver volume was measured by spiral CT. Data were estimated by t-test, variance calculation and chi-squared test. The relationships between all these parameters and different groups were investigated according to Child-Pugh classification and postoperative complications respectively.RESULTS: Steady blood concentration was achieved 120 mins after D-sorbitol intravenous infusion, which was (0.358±0.064) mmoⅠ@L-1 in cirrhotic group and (0.189±0.05)mmol@L-1 in control group (P<0.01). CLH=(812.7±112.4) ml@min-1,QTOTAL=(1280.6±131.4) ml@min-1, and RINS=(36.54±10.65)%in cirrhotic group and CLH=(1248.3±210.5) ml.min-1, QTOTAL=(1362.4-±126.9) ml@min-1, and RINS=(8.37±3.32) % in control group (P<0.01). The liver volume of cirrhotic group was 1057±249 cm3, 851±148 cm3 and 663±77 cm3 in Child A, B and C group respectively with significant difference (P<0.001).The average volume of cirrhotic liver in Child B, C group was significantly reduced in comparison with that in control group (P<0.001). The patient, whose liver volume decreased by 40 % with the CLH below 600 ml@min-1, would have a higher incidence of postoperative complications. There was no strict correspondent relationship between CLH, liver volume and Child-Pugh′s classification.CONCLUSION: The hepatic clearance of D-sorbitol, CT measured liver volume can be reliably used for the evaluation of hepatic functional blood flow and liver metabolic volume. Combined with the Child-Pugh′s classification, it could be very useful for further understanding the liver functional reserve, therefore help determine reasonable therapeutic plan, choose surgical procedures and operating time. | Yi-Ming Li Fan Lv Xin Xu Hong Ji Wen-Tao Gao Tuan-Jie Lei Gui-Bing Ren Zhi-Lan Bai Qiang Li General Surgery Department,the 2~(nd)Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,ShaanXi Province,China | 2003 | World Journal of Gastroenterology2003，9，9： | 16 |
| 7 | The Chromosome-Based Rubber Tree Genome Provides New Insights into Spurge Genome Evolution and Rubber Biosynthesis显示文摘The rubber tree,Hevea brasiliensis,produces natural rubber that serves as an essential industrial raw material.Here,we present a high-quality reference genome for a rubber tree cultivar GT1 using single-molecule real-time sequencing(SMRT)and Hi-C technologies to anchor the~1.47-Gb genome assembly into 18 pseudochromosomes.The chromosome-based genome analysis enabled us to establish a model of spurge chromosome evolution,since the common paleopolyploid event occurred before the split of Hevea and Manihot.We show recent and rapid bursts of the three Hevea-specific LTR-retrotransposon families during the last 10 million years,leading to the massive expansion by~65.88%(~970 Mbp)of the whole rubber tree genome since the divergence from Manihot.We identify large-scale expansion of genes associated with whole rubber biosynthesis processes,such as basal metabolic processes,ethylene biosynthesis,and the activation of polysaccharide and glycoprotein lectin,which are important properties for latex production.A map of genomic variation between the cultivated and wild rubber trees was obtained,which contains~15.7 million high-quality single-nucleotide polymorphisms.We identified hundreds of candidate domestication genes with drastically lowered genomic diversity in the cultivated but not wild rubber trees despite a relatively short domestication history of rubber tree,some of which are involved in rubber biosynthesis.This genome assembly represents key resources for future rubber tree research and breeding,providing novel targets for improving plant biotic and abiotic tolerance and rubber production. | Jin Liu Cong Shi Cheng-Cheng Shi Wei Li Qun-Jie Zhang Yun Zhang Kui Li Hui-Fang Lu Chao Shi Si-Tao Zhu Zai-Yun Xiao Hong Nan Yao Yue Xun-Ge Zhu Yu Wu Xiao-Ning Hong Guang-Yi Fan Yan Tong Dan Zhang Chang-Li Mao Yun-Long Liu Shi-Jie Hao Wei-Qing Liu Mei-Qi Lv Hai-Bin Zhang Yuan Liu Ge-Ran Hu-tang Jin-Peng Wang Jia-Hao Wang Ying-Huai Sun Shu-Bang Ni Wen-Bin Chen Xing-Cai Zhang Yuan-Nian Jiao Evan E.Eichler Guo-Hua Li Xin Liu Li-Zhi Gao | 2020 | Molecular Plant2020，13，2： | 15 |
| 8 | Soil Fertility,Microbial Biomass,and Microbial Functional Diversity Responses to Four Years Fertilization in an Apple Orchard in North China显示文摘Soil microbial communities play an essential role in maintaining soil fertility and are considered as ecological indicators to evaluate soil health.In the present study,we examined the influence of almost 4 years of fertilization[no fertilizer(CK),nitrogen alone(N),nitrogen,phosphorus and potassium chemical fertilizer(NPK),organicmanure(M),nitrogen plus organic manure(NM),and NPK plus organic manure(NPKM)]on soil fertility and the functional diversity of soil microbial communities in an apple orchard.Compared to CK,fertilization increased soil organic carbon,total nitrogen,and available nutrients,but reduced soil pH in N and NPK treatments.The highest microbial biomass carbon and nitrogen,most probable number of actinomycetes,bacteria,and fungi occurred in the NPKM treatment.The average well color development(AWCD)values followed the order of NPKM>M>NPK and NM>CK and N.The Shannon index in organic manure treatments were significantly higher than in control and in treatments without organic manure.The principal component analysis showed that manure treatment was significantly separated from other treatments.These results indicated that organic manure applied alone or in combination with chemical fertilizers would increase soil fertility and functional diversity of soil microbial communities.Moreover,applying balanced N,P,K fertilizer in combination with organic manure was found to be superior to the use of a single fertilizer in improving soil microbial community quality. | Zhanling Zhu Yan Bai Minglu Lv Ge Tian Xin Zhang Li Li Yuanmao Jiang Shunfeng Ge | 2020 | Horticultural Plant Journal2020，6，4： | 13 |
| 9 | Electroacupuncture at Dazhui(GV14) and Mingmen(GV4) protects against spinal cord injury:the role of the Wnt/β-catenin signaling pathway显示文摘Electroacupuncture at Dazhui(GV14) and Mingmen(GV4) on the Governor Vessel has been shown to exhibit curative effects on spinal cord injury; however, the underlying mechanism remains poorly understood. In this study, we established rat models of spinal cord injury using a modified Allen's weight-drop method. Ninety-nine male Sprague-Dawley rats were randomly divided into three equal groups: sham(only laminectomy), SCI(induction of spinal cord injury at T10), and EA(induction of spinal cord injury at T10 and electroacupuncture intervention at GV14 and GV4 for 20 minutes once a day). Rats in the SCI and EA groups were further randomly divided into the following subgroups: 1-day(n = 11), 7-day(n = 11), and 14-day(n = 11). At 1, 7, and 14 days after electroacupuncture treatment, the Basso, Beattie and Bresnahan locomotor rating scale showed obvious improvement in rat hind limb locomotor function, hematoxylin-eosin staining showed that the histological change of injured spinal cord tissue was obviously alleviated, and immunohistochemistry and western blot analysis showed that Wnt1, Wnt3 a, β-catenin immunoreactivity and protein expression in the injured spinal cord tissue were greatly increased compared with the sham and SCI groups. These findings suggest that electroacupuncture at GV14 and GV4 upregulates Wnt1, Wnt3 a, and β-catenin expression in the Wnt/β-catenin signaling pathway, exhibiting neuroprotective effects against spinal cord injury. | Xin Wang Su-hua Shi Hai-jiang Yao Quan-kai Jing Yu-ping Mo Wei Lv Liang-yu Song Xiao-chen Yuan Zhi-gang Li Li-na Qin | 2016 | Neural Regeneration Research2016，11，12： | 13 |
| 10 | Use of tailored loading-dose clopidogrel in patients undergoing selected percutaneous coronary intervention based on adenosine diphosphate-mediated platelet aggregation显示文摘背景腺苷调停磷酸盐的血小板聚集是为在经历了选择经皮的冠的干预的病人的主要不利心脏的事件的一个预示的因素。试图估计 clopidogrel 的调整装载剂量是否能更有效地在经历的病人禁止血小板聚集的这研究选择了经皮的冠的 intervention.Methods 经历选择经皮的冠的干预的 205 个病人的一个总数在这 multicenter 被注册,未来的、使随机化的学习。收到国内 clopidogrel (n=104 ) 的病人担任了 Talcom (Taijia ) 组;其它(n=101 ) 收到了 Plavix, Plavix 组,病人们收到了腺苷直到装载 clopidogrel 的剂量到减少的 3 另外的 300-mg 在超过 50% 的调停磷酸盐的血小板聚集索引(主要端点) 与基线相比。第二等的端点与合理装载剂量相比是在 12 months.Results 的主要不利心血管的事件,定制的装载剂量更好在腺苷基于 50% 减少禁止了血小板聚集调停磷酸盐的血小板聚集(合理装载剂量对定制的装载剂量, 48% 对 73% , P=0.028 ) 。在在 Talcom 和 Plavix 组之间的合格索引没有重要差别(47% 对 49% 在 300 mg;62% 对 59% 在 600 mg;74% 对 72% 在 900 mg;P 0.05 ) 基于标准腺苷 50% 的调停 diphosphate 的血小板聚集减少。在 12 月后续以后,在主要不利心脏的事件没有重要差别(2.5% 对 2.9% , P=5.43 ) 。没有尖锐或稍尖的 stent 血栓事件 occurred.Conclusion clopidogrel 的调整装载剂量能与合理剂量相比在 antiplatelet 聚集上有重要效果,减少的 1 年的专业在基于腺苷经历经皮的冠的干预的病人的不利心脏的事件没有流血的增加的调停磷酸盐的血小板聚集。 | MENG Kang LV Shu-zheng ZHU Hua-gang CHEN Xin GE Chang-jiang SONG Xian-tao | 2010 | Chinese Medical Journal2010，，24： | 11 |
| 11 | A static pressure sensitive receptor APJ promote H9c2 cardiomyocyte hypertrophy via PI3K-autophagy pathway显示文摘这研究被设计调查 APJ 受体是否在静态的导致压力的 cardiomyocyte hypertrophyand 充当一个传感器调查 PI3K-autophagy 小径的机制。左室的肥大老鼠模型是腹的主动脉的确定的 bycoarctation。H9c2 老鼠 cardiomyocytes 面对被给 bya 的静态的压力是有教养的定做的压力孵卵器。结果表明 apelin/APJ 系统, PI3K, Akt 和他们的 phosphorylationwere 的表达式显著地在操作组增加了。静态的压力起来调整在 cardiomyocytes 的 APJ 表示, PI3K phosphorylation, Akt phosphorylation, LC3-II/I 和 beclin-1 表示。APJ shRNA pGPU6/Neo-rat-399, PI3K 禁止者 LY294002, Akt 禁止者 1701-1 分别地堵住了 APJ, PI3K phosphorylation, Akt phosphorylation, LC3-II/I 和 beclin-1 表示的起来规定。而且,静态的压力增加了直径,体积,房间的蛋白质内容,和这些能是 reversedwhen 房间分别地与 pGPU6/Neo-rat-399, LY294002,和 autophagy 禁止者 3-methyladenine 被对待。Theseresults 建议了那静态的压力起来调整 APJ 表情由 PI3K-autophagypathway 支持 cardiomyocyte 肥大。 | Feng Xiei Wei Liu Fen Feng Xin Li Li Yang Deguan Lv Xuping Qin Lanfang Li Linxi Chen | 2014 | Acta Biochimica et Biophysica Sinica2014，46，8： | 10 |
| 12 | Apelin-13 promotes cardiomyocyte hypertrophy via PI3K-Akt-ERK1/2-p70S6K and PI3K-induced autophagy显示文摘Apelin 高度在左室的肥大 Sprague Dawley 老鼠建模的老鼠被表示,并且它在心血管的系统起一个关键作用。这研究是澄清 apelin-13 是否在 H9c2 老鼠 cardiomyocytes 支持肥大并且调查它的内在的机制的目的。cardiomyocyte 肥大被测量 H9c2 房间的直径,体积,和蛋白质内容观察。autophagy 的激活被由传播电子显微镜学观察 autophagosomes 的形态学评估,观察由轻显微镜学的 LC3 的 subcellular 本地化,并且由西方的污点分析检测 LC3 的联系膜的形式。表明小径的 phosphatidylinositol 3-kinase (PI3K ) 被识别,蛋白质表示用西方的污点分析被检测。结果表明 apelin-13 增加了 H9c2 房间的直径,体积,和蛋白质内容并且支持了 PI3K, Akt, ERK1/2,和 p70S6K 的 phosphorylation。Apelin-13 激活 PI3K-Akt-ERK1/2-p70S6K 小径。PI3K 禁止者 LY294002, Akt 禁止者 1701-1, ERK1/2 禁止者 PD98059 稀释了房间直径的增加,体积,蛋白质内容由 apelin-13 导致了。Apelin-13 增加了 autophagosomes 并且起来调整 beclin 的表情 1 并且 LC3-II/I 短暂地并且稳定地。autophagy 禁止者 3MA 改善了被 apelin-13 导致的房间直径,体积,和蛋白质内容的增加。这些结果建议 apelin-13 经由 PI3K-Akt-ERK1/2-p70S6K 和导致 PI3K 的 autophagy 支持 H9c2 老鼠 cardiomyocyte 肥大。 | Feng Xie Wei Liu Fen Feng Xin Li Lu He Deguan Lv Xuping Qin Lifang Li Lanfang Li Linxi Chen | 2015 | Acta Biochimica et Biophysica Sinica2015，47，12： | 9 |
| 13 | Radiomics for the detection of microvascular invasion in hepatocellular carcinoma显示文摘Hepatocellular carcinoma(HCC)is the most common primary liver cancer,accounting for about 90%of liver cancer cases.It is currently the fifth most common cancer in the world and the third leading cause of cancer-related mortality.Moreover,recurrence of HCC is common.Microvascular invasion(MVI)is a major factor associated with recurrence in postoperative HCC.It is difficult to evaluate MVI using traditional imaging modalities.Currently,MVI is assessed primarily through pathological and immunohistochemical analyses of postoperative tissue samples.Needle biopsy is the primary method used to confirm MVI diagnosis before surgery.As the puncture specimens represent just a small part of the tumor,and given the heterogeneity of HCC,biopsy samples may yield false-negative results.Radiomics,an emerging,powerful,and non-invasive tool based on various imaging modalities,such as computed tomography,magnetic resonance imaging,ultrasound,and positron emission tomography,can predict the HCC-MVI status preoperatively by delineating the tumor and/or the regions at a certain distance from the surface of the tumor to extract the image features.Although positive results have been reported for radiomics,its drawbacks have limited its clinical translation.This article reviews the application of radiomics,based on various imaging modalities,in preoperative evaluation of HCC-MVI and explores future research directions that facilitate its clinical translation. | Kun Lv Xin Cao Peng Du Jun-Yan Fu Dao-Ying Geng Jun Zhang | 2022 | World Journal of Gastroenterology2022，28，20： | 9 |
| 14 | Effects of cotton straw amendment on soil fertility and microbial communities显示文摘 | Wuren HUANG Zhihui BAI Daniel HOEFEL Qing HU Xin LV Guoqiang ZHUANG Shengjun XU Hongyan QI Hongxun ZHANG | 2012 | Frontiers of Environmental Science & Engineering2012，6，3： | 8 |
| 15 | Immediate implant placement in combination with platelet rich-fibrin into extraction sites with periapical infection in the esthetic zone:A case report and review of literature显示文摘BACKGROUND In this case,platelet-rich fibrin(PRF)was added to guided tissue regeneration as a biomaterial in proper order for immediate planting in aesthetic area with periapical infection.CASE SUMMARY With the history of endodontic failure in maxillary central incisor,a 34-year-old female patient required the extraction of maxillary anterior residual root and immediate implantation.Cone beam computed tomography and clinical observation were used to assess the regeneration of soft and bone tissue.Before operation,cone beam computed tomography showed the anterior residual root had serious periapical periodontitis with insufficient labial bone in the aesthetic zone.The patient underwent immediate implant placement and reconstruction of the bone substitution by modified guided bone regeneration.The barrier was a three-layer structure of PRF-collagen membrane-PRF that covered the mixture of PRF and Bio-Oss to promote both osteogenesis and soft tissue healing.At 6 mo postoperatively,the definitive crown was placed after accomplished finial impression.One-year follow-up showed a satisfactory aesthetic effect with no obvious absorption of the labial bone and soft tissue.CONCLUSION The use of PRF in combination with guided bone regeneration can serve as a reliable and simple adjuvant for immediate implanting in infected socket and result in a stable osteogenic effect with good aesthetic outcome. | Jiao Fang Xi-Rui Xin Wen Li Han-Chi Wang Hui-Xin Lv Yan-Min Zhou | 2021 | World Journal of Clinical Cases2021，9，4： | 7 |
| 16 | “Three Methods and Three Points” regulates p38 mitogen-activated protein kinase in the dorsal horn of the spinal cord in a rat model of sciatic nerve injury显示文摘Tuina is a traditional Chinese treatment for sensory disturbances caused by peripheral nerve injury and related diseases. Our previous studies showed that tuina regulates relevant regions and indices of the spinal dorsal horn using the Dian, Bo, and Rou method in Yinmen(BL37), Yanglingquan(GB34), and Weizhong(BL40). Treatment prevents muscle atrophy, protects spinal cord neurons, and promotes sciatic nerve repair. The mechanisms of action of tuina for treating peripheral nerve injury remain poorly understood. This study established rat models of sciatic nerve injury using the crushing method. Rats received Chinese tuina in accordance with the principle of 'Three Methods and Three Points,' once daily for 20 days. Tuina intervention reduced paw withdrawal latency and improved wet weight of the gastrocnemius muscle, as well as promoting morphological recovery of sciatic nerve fibers, Schwann cells, and axons. The protein expression levels of phospho-p38 mitogen-activated protein kinase, tumor necrosis factor-α, and interleukin-1β also decreased. These findings indicate that 'Three Methods and Three Points' promoted morphological recovery and improved behavior of rats with peripheral nerve injury. | Xin Guo Tian-yuan Yu Wong Steven Wen-duan Jia Chi Ma Yan-hong Tao Chao Yang Tao-tao Lv Shuai Wu Meng-qian Lu Jia-li Liu | 2016 | Neural Regeneration Research2016，11，12： | 7 |
| 17 | Lipid analysis of three special nervonic acid resources in China显示文摘Nervonic acid plays an important role in nutrition and function of the human body.Malania oleifera,Acer truncatum and Xanthoceras sorbifolium are China’s unique woody plant rich in nervonic acid in seed oil.This study aims to investigate the lipid composition of these 3 special resources.Their fatty acids were detected by gas chromatography coupled with flame detector(GC-FID).Triglycerides(TAGs)and phospholipids(PLs)were detected by shotgun-mass spectrometry(shotgun-MS).Results showed that M.oleifera oil presented the highest level of nervonic acid(46.20±0.22%)among the 3 oils.Seeds oil of A.truncatum and X.sorbifolium had 3.53±0.20%and 1.83±0.21%nervonic acid respectively.53 species of TAGs and 15 species of PLs were identified in M.oleifera oil,with PLs content of 499.94±22.34μg/g.In A.truncatum oil,PL and TAG species were twice more than those in M.oleifera oil,and its’content of PLs was 76.27±3.21μg/g.In X.sorbifolium oil,75 TAGs and 34 PLs were detected,with the lowest PLs at 23.84±0.17μg/g.The results demonstrated that these 3 vegetable oils have great potential to become nervonic acid supplements for human health. | Xinghao Tu Jingya Wan Ya Xie Fang Wei Siewyoung Quek Xin Lv Liqing Du Hong Chen | 2020 | Oil Crop Science2020，5，4： | 7 |
| 18 | Association of genotypes of rs671 within ALDH2 with risk for gastric cardia adenocarcinoma in the Chinese Han population in high-and low-incidence areas显示文摘Objective: This study aimed to determine if gastric cardia adenocarcinoma(GCA) risk was associated with the lys(A or *2) allele at the rs671(glu504lys) polymorphism within the aldehyde dehydrogenase 2(ALDH2) gene in a Chinese Han population. We also aimed to investigate ALDH2 genotypic distributions between subjects from high- and low-incidence areas for both GCA and esophageal squamous cell carcinoma(ESCC).Methods: We designed a case-control study including 2,686 patients with GCA and 3,675 control subjects from high- and lowincidence areas for both GCA and ESCC in China. Taq Man allele discrimination assay was used to genotype the rs671 polymorphism. χ~2 test and binary logistic regression analysis were used to estimate the odds ratios for the development of GCA,and multivariate ordinal logistic regression was used to analyze ALDH2 genotypic distributions among different groups.Results: Compared with ALDH2*1/*1 homozygotes, ALDH2*1/*2 and ALDH2*2/*2 carriers did not increase the risk for GCA in the Chinese Han population(P>0.05). Interestingly, the ratio of homozygous or heterozygous ALDH2 *2 carriers in highincidence areas for both GCA and ESCC was lower than that in low-incidence areas(P<0.001).Conclusions: Genotypes of rs671 at ALDH2 may not increase GCA susceptibility in Chinese Han populations. In addition, the ALDH2 genotypic distribution differs between Chinese Han populations from high- and low-incidence areas for both GCA and ESCC. Our findings may shed light on the possible genetic mechanism for the dramatic geographic differences of GCA occurrence in China. | Lian-Qun Zhang Xin Song Xue-Ke Zhao Jia Huang Peng Zhang Lu-Wen Wang Hui Meng Jian-Wei Ku Guo-Qiang Kong Tao Jiang Xin-Min Li Xiao-Long Lv Teng Ma Guo Yuan Min-Jie Wu Shou-Jia Hu Shuang Lv Tang-Juan Zhang Ling-Fen Ji Zong-Min Fan Neng-Chao Wang Yao-Wen Zhang Fu-You Zhou Li-Dong Wang | 2017 | Cancer Biology & Medicine2017，14，1： | 7 |
| 19 | From nanoparticles to single atoms for Pt/CeO2:Synthetic strategies,characterizations and applications显示文摘Pt/CeO2 catalysts with unitary Pt species,nanoparticles,clusters or single atoms,often exhibit excellent activity and unique selectivity in many catalytic reactions benefiting from their small size,abundant unsaturated active sites,and unique electro nic structure.In recent years,a tre mendous number of related articles have provided great inspiration to future research and development of Pt/CeO2 catalysts.In this review,the state-of-the-art evolution of Pt nanoparticles to Pt single atoms on CeO2 is reviewed with the emphasis on synthetic strategies,advanced characterization techniques(allowing one to clarify the single atoms from clusters),the catalytic applications and mechanisms from the viewpoint of theoretical calculation.Finally,the critical outlooks and the challenges faced in developing the single-atom Pt/CeO2 catalysts are highlighted. | Ying Xin Nana Zhang Yanan Lv Jin Wang Qian Li Zhaoliang Zhang | 2020 | Journal of Rare Earths2020，38，8： | 7 |
| 20 | bHLH transcription factor SmbHLH92 negatively regulates biosynthesis of phenolic acids and tanshinones in Salvia miltiorrhiza显示文摘Objective:Salvia miltiorrhiza is a valuable herbal medicine with tanshinone and phenolic acid as the main biological active ingredients.The biosynthetic regulation of these bioactive compounds is controlled by a set of transcription factors(TFs).The basic helix-loop-helix(bHLH)transcription factor plays an important role in various physiological and biochemical processes in plants.However,research on bHLH TFs regulating phenolic acid or tanshinone biosynthesis in S.miltiorrhiza is limited.Methods:qRT-PCR was used for gene expression analysis.The subcellular localization of SmbHLH92 was detected by SmbHLHg2-GFP transient transformation into tobacco leaves,and its fluorescence was observed using a confocal laser scanning microscope.The transcriptional activity of SmbHLH92 was confirmed in the AH109 yeast strain.RNA interference hairy roots of SmbHLH92-RNAi transgenic lines were obtained through Agrobacterium-mediated genetic transformation.Ultra performance liquid chromatography(UPLC)was used to detect the changes of phenolic acids and tanshinones.Results:SmbHLH92 is a bHLH transcription factor that is highly expressed in the root and phloem of S.miltiorrhiza.The subcellular localization and transcriptional activity of SmbHLH92 indicated that SmbHLH92 was located in the nucleus and may be a transcription factor.RNA interference(RNAi)of SmbHLH92 in hairy roots of S.miltiorrhiza significantly increased the accumulation of phenolic acid and tanshinone.Quantitative RT-PCR(RT-qPCR)analysis showed the transcription level of genes encoding the key enzymes involved in the phenolic acid and tanshinone biosynthetic pathways was increased in the hairy roots of the SmbHLH92-RNAi transgenic line,comparing with the control line.Conclusion:These data indicate that SmbHLH92 is a negative regulator involved in the regulation of phenolic acid and tanshinone biosynthesis in S.miltiorrhiza. | Jian-hong Zhang Hai-zhou Lv Wan-jing Liu Ai-jia Ji Xin Zhang Jing-yuan Song Hong-mei Luo Shi-lin Chen | 2020 | Chinese Herbal Medicines2020，12，3： | 7 |
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