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| 1 | Hepatitis C virus: Morphogenesis, infection and therapy显示文摘Hepatitis C virus(HCV) is a major cause of liver diseases including liver cirrhosis and hepatocellular carcinoma. Approximately 3% of the world population is infected with HCV. Thus, HCV infection is considered a public healthy challenge. It is worth mentioning, that the HCV prevalence is dependent on the countries with infection rates around 20% in high endemic countries. The review summarizes recent data on HCV molecular biology, the physiopathology of infection(immune-mediated liver damage, liver fibrosis and lipid metabolism), virus diagnostic and treatment. In addition, currently available in vitro, ex vivo and animal models to study the virus life cycle, virus pathogenesis and therapy are described. Understanding of both host and viral factors may in the future lead to creation of new approaches in generation of an efficient therapeutic vaccine. | Vladimir Alexei Morozov Sylvie Lagaye | 2018 | World Journal of Hepatology2018，10，2： | 7 |
| 2 | Anti-hepatitis C virus potency of a new autophagy inhibitor using human liver slices model显示文摘AIM: To evaluate the antiviral potency of a new antihepatitis C virus(HCV) antiviral agent targeting the cellular autophagy machinery. METHODS: Non-infected liver slices, obtained from human liver resection and cut in 350 μm-thick slices(2.7 × 106 cells per slice) were infected with cell culture-grown HCV Con1b/C3 supernatant(multiplicity of infection = 0.1) cultivated for up to ten days. HCV infected slices were treated at day 4 post-infection with GNS-396 for 6 d at different concentrations. HCV replication was evaluated by strand-specific real-time quantitative reverse transcription- polymerase chain reaction. The infectivity titers of supernatants were evaluated by foci formation upon inoculation into naive Huh-7.5.1 cells. The cytotoxic effect of the drugs was evaluated by lactate dehydrogenase leakage assays. RESULTS: The antiviral efficacy of a new antiviral drug, GNS-396, an autophagy inhibitor, on HCV infection of adult human liver slices was evidenced in a dosedependent manner. At day 6 post-treatment, GNS-396 EC50 was 158 nmol/L without cytotoxic effect(compared to hydroxychloroquine EC50 = 1.17 μmol/L).CONCLUSION: Our results demonstrated that our ex vivo model is efficient for evaluation the potency of autophagy inhibitors, in particular a new quinoline derivative GNS-396 as antiviral could inhibit HCV infection in a dosedependent manner without cytotoxic effect. | Sylvie Lagaye Sonia Brun Jesintha Gaston Hong Shen Ruzena Stranska Claire Camus Clarisse Dubray Géraldine Rousseau Pierre-Philippe Massault Jerome Courcambeck Firas Bassisi Philippe Halfon Stanislas Pol | 2016 | World Journal of Hepatology2016，8，21： | 5 |
| 3 | European network forthe study of in utero transmission of HIV-1 cell-to-cell contactresults in a selective translocation of maternal human immuno-deficiency virus type 1 quasispecies across a trophoblastic bar-rier by both transcytosis and infection显示文摘 | Lagaye S Derrien M Menu E | 2001 | J Virol2001，75，10： | 1 |
| 4 | Cell-to-cell contact results in a selective translocation of maternal human immunodeficiency virus type 1 quasispecies across a trophoblastic barrier by both transcytosis and infection显示文摘 | Lagaye S Derrien M Menu E | 2001 | J Virol2001，75，10： | 1 |
| 5 | Cell-to-cell contact resuits in a selective translocation of maternal human iimmunodeficiency virus type 1 quasipecies across a trophoblastic barrier by both transcytosis and infection显示文摘 | LAGAYE S DERRIEN M MENU E | 2002 | J Virol2002，75，10： | 1 |
| 6 | Efficient replication of primary or culture hepatitis C virus isolates in human liver slices: A relevant ex vivo model of liver infection显示文摘 | Sylvie Lagaye Hong Shen Bertrand Saunier Michelina Nascimbeni Jesintha Gaston Pierre Bourdoncle Laurent Hannoun Pierre‐Philippe Massault Ana?s Vallet‐Pichard Vincent Mallet Stanislas Pol | 2012 | Hepatology2012，，3： | 1 |
| 7 | Endogenous JSRV-like proviruses in domestic cattle:Analysis of sequences and transcripts显示文摘 | MOROZOV V A MOROZOV A V LAGAYE S | 2007 | Virology2007，367，1： | 1 |
| 8 | Shortbowelsyndrome:epidemiologyandetiology显示文摘 | walesPw ChristisonLagayER | 2010 | SeminPediatrSurg2010，19，1： | 1 |
| 9 | NK T cell-plasmacytoid dendritic cell cooperation via OX40 controls viral infection in a tissue-specific manner显示文摘 | Diana J Griseri T Lagaye S | 2009 | Immunity2009，30，2： | 1 |
| 10 | Human spumaretrovims-related sequences in the DNA of leukocytes from patients With Graves Graves' disease显示文摘 | Lagaye S Vexiau P Morozov V | 1992 | Proc Nail Acad Sci USA1992，89，10： | 1 |
| 11 | Human spumaretrovirus-related sequences in the DNA of leukocytes from patients with Graves disease显示文摘 | Lagaye S Vexiau P Morozov V | 1992 | Proc Natl Acad Sci U S A1992，89，10： | 1 |
| 12 | NKT cell-plasmaeytoid dendritic cell cooperation via OX40 controls viral infection in a tissue- specific manner显示文摘 | Diana J Griseri T Lagaye S | 2009 | Immunity2009，30，2： | 1 |
| 13 | Adult human liver slice cultures:Modelling of liver fibrosis and evaluation of new anti-fibrotic drugs显示文摘BACKGROUND Liver fibrosis can result in end-stage liver failure and death.AIM To examine human liver fibrogenesis and anti-fibrotic therapies,we evaluated the three dimensional ex vivo liver slice(LS)model.METHODS Fibrotic liver samples(F0 to F4 fibrosis stage according to the METAVIR score)were collected from patients after liver resection.Human liver slices(HLS)were cultivated for up to 21 days.Hepatitis C virus(HCV)infection,alcohol(ethanol stimulation)and steatosis(palmitate stimulation)were examined in fibrotic(F2 to F4)liver slices infected(or not)with HCV.F0-F1 HLS were used as controls.At day 0,either ursodeoxycholic acid(choleretic and hepatoprotective properties)and/or α-tocopherol(antioxidant properties)were added to standard of care on HLS and fibrotic liver slices,infected(or not)with HCV.Expression of the biomarkers of fibrosis and the triglyceride production were checked by quantitative reverse transcription polymerase chain reaction and/or enzymelinked immunosorbent assay.RESULTS The cultures were viable in vitro for 21 days allowing to study fibrosis inducers and to estimate the effect of anti-fibrotic drugs.Expression of the biomarkers of fibrosis and the progression to steatosis(estimated by triglycerides production)was increased with the addition of HCV and/or ethanol or palmitate.From day 15 of the follow-up studies,a significant decrease of both transforming growth factorβ-1 and Procol1A1 expression and triglycerides production was observed when a combined anti-fibrotic treatment was applied on HCV infected F2-F4 LS cultures.CONCLUSION These results show that the human three dimensional ex vivo model effectively reflects the in vivo processes in damaged human liver(viral,alcoholic,nonalcoholic steatohepatitis liver diseases)and provides the proof of concept that the LS examined model permits a rapid evaluation of new anti-fibrotic therapies when used alone or in combination. | Daria Kartasheva-Ebertz Jesintha Gaston Loriane Lair-Mehiri Pierre-Philippe Massault Olivier Scatton Jean-Christophe Vaillant Vladimir Alexei Morozov Stanislas Pol Sylvie Lagaye | 2021 | World Journal of Hepatology2021，13，2： | 0 |
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