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| 1 | Innovative immunohistochemistry identifies MMP-9 expressing macrophages at the invasive front of murine HCC显示文摘AIM:To investigate the proteolytic contribution of tumor-associated macrophages(TAM)in tumor invasion,we analyzed whether TAM at the invasive front of small HCC in Abcb4-/--mice show an enhanced expression of MMP-9. METHODS:Liver cryosections of the hepatocellular carcinoma(HCC)invasive front from 12 mo old Abcb4-/--mice were stained for collagen typeⅠand MMP-9 using Alexa488 and Alexa568 labeled secondary antibodies.Afterwards,the Alexa568 dye was bleached and the macrophage marker F4/80 was visualized using Alexa568 labeled secondary antibodies.Finally, photographs of the invasive tumor front were digitally overlaid and analyzed. RESULTS:After complete bleaching of the primary dye,specific fluorescence staining of a third antigen, here F4/80,was successfully performed on the same histological section.With this method,we were able to identify conglomerates of matrix metalloproteinase (MMP-9)expressing macrophages within the tumor capsule of HCC. CONCLUSION:MMP-9 expressing macrophages are involved in matrix remodelling at the invasive tumor front of HCC.The described staining protocol provides a simple yet powerful extension of conventional immuno-histochemistry,facilitating visualization of at least three different antigens plus nuclei in one single histological section. | Martin Roderfeld Timo Rath Frank Lammert Christian Dierkes Jürgen Graf Elke Roeb | 2010 | World Journal of Hepatology2010，2，5： | 6 |
| 2 | Cholesterol crystal binding of biliary immunoglobulin A: visualization by fluorescence light microscopy显示文摘AIM To assess potential contributions of biliary IgA for crystal agglomeration into gallstones, we visualized cholesterol crystal binding of biliary IgA.METHODS Crystal-binding biliary proteins were extracted from human gallbladder bile using lectin affinity chromatography. Biliary IgA was isolated from the bound protein fraction by immunoaffinity chromatography. Pure cholesterol monohydrate crystals were incubated with biliary IgA and fluoresceine isothiocyanate (FITC)-conjugated anti-lgA at 37C. Samples were examined under polarizing and fluorescence light microscopy with digital image processing.RESULTS Binding of biliary IgA to cholesterol monohydrate crystals could be visualized with FITC-conjugated anti-lgA antibodies. Peak fluorescence occurred at crystal edges and dislocations. Controls without biliary IgA or with biliary IgG showed no significant fluorescence.CONCLUSION Fluorescence light microscopy provided evidence for cholesterol crystal binding of biliary IgA. Cholesterol crystalbinding proteins like IgA might be important mediators of crystal agglomeration and growth of cholesterol gallstones by modifying the evolving crystal structures in vivo. | Frank Lammert Stefan Südfeld Norbert Busch Siegfried Matern | 2001 | World Journal of Gastroenterology2001，7，2： | 6 |
| 3 | Nonselective betablocker therapy decreases intestinal permeability and serum levels of LBP and IL-6 in patients with cirrhosis显示文摘 | T. Reiberger A. Ferlitsch B.A. Payer M. Mandorfer B.B. Heinisch H. Hayden F. Lammert M. Trauner M. Peck-Radosavljevic H. Vogelsang | 2012 | Journal of Hepatology2012，，： | 5 |
| 4 | COMPARING PERFORMANCE OF CROP SPECIES MIXTURES AND PURE STANDS显示文摘Intercropping is the planned cultivation of species mixtures on agricultural land.Intercropping has many attributes that make it attractive for developing a more sustainable agriculture,such as high yield,high resource use efficiency,lower input requirements,natural suppression of pests,pathogens and weeds,and building a soil with more organic carbon and nitrogen.Information is needed which species combinations perform best under different circumstances and which management is suitable to bring out the best from intercropping in a given production situation.The literature is replete with case studies on intercropping from across the globe,but evidence synthesis is needed to make this information accessible.Meta-analysis requires a careful choice of metric that is appropriate for answering the question at hand,and which lends itself for a robust meta-analysis.This paper reviews some metrics that may be used in the quantitative synthesis of literature data on intercropping. | Wopke VAN DER WERF Lizhen ZHANG Chunjie LI Ping CHEN Chen FENG Zhan XU Chaochun ZHANG Chunfeng GU Lammert BASTIAANS David MAKOWSKI TjeerdJan STOMPH | 2021 | Frontiers of Agricultural Science and Engineering2021，8，3： | 2 |
| 5 | The Genetics of Complex Cholestatic Disorders显示文摘 | Gideon M. Hirschfield Roger W. Chapman Tom H. Karlsen Frank Lammert Konstantinos N. Lazaridis Andrew L. Mason | 2013 | Gastroenterology2013，，7： | 2 |
| 6 | Effect of thrombocytopenia on treatment tolerability and outcome in patients with chronic HCV infection and advanced hepatic fibrosis显示文摘 | Raoel Maan Adriaan J. van der Meer Bettina E. Hansen Jordan J. Feld Heiner Wedemeyer Jean-Fran?ois Dufour Hooman F. Zangneh Frank Lammert Michael P. Manns Stefan Zeuzem Harry L.A. Janssen Robert J. de Knegt Bart J. Veldt | 2014 | Journal of Hepatology2014，，： | 2 |
| 7 | Variant adiponutrin (PNPLA3) represents a common fibrosis risk gene: Non-invasive elastography-based study in chronic liver disease显示文摘 | Marcin Krawczyk Frank Grünhage Vincent Zimmer Frank Lammert | 2011 | Journal of Hepatology2011，，2： | 2 |
| 8 | The vascular niche and its basement membrane显示文摘 | Nikolova G Strilic B Lammert E | 2007 | Trends Cell Biol2007，17，1： | 1 |
| 9 | Genetic risk factors for hepatic fibrosis in chronic liver diseases 显示文摘 | WASMUTH HE LAMMERT F MATERN S | 2003 | Med Klin2003，98，12： | 1 |
| 10 | Precision demanded of a rowland circle monochromator: its realization显示文摘 | Senf F Lammert H Flechsig U | 1995 | Rev Sci Instrum1995，66，2： | 1 |
| 11 | Biliary secretory immunoglobulin A is a major of the new group of cholesterol crystal-binding proteins显示文摘 | Busch N Lammert F Matern S | 1998 | Gastroenterology1998，115，1： | 1 |
| 12 | 'Giving and taking' : endothelial and beta-cells in the islets of Langerhans 显示文摘 | Eberhard D Kragl M Lammert E | 2010 | Trends Endocrinol Metab2010，21，： | 1 |
| 13 | Induction of pancreatic differentiation by signals from blood vessels显示文摘 | ECKHARD LAMMERT ONDINE CLEAVER DOUGLAS MELTON | 2001 | Science2001，294，： | 1 |
| 14 | Environmental riskmapping of pollu- tants : State of the art and communication aspects显示文摘 | JOOST L LAMMERT K | 2010 | Science of the Total Environment2010，408，18： | 1 |
| 15 | Gallstones:environment,lifestyle and genes显示文摘 | Stokes CS Krawczyk M Lammert F | 2011 | Dig Dis2011，29，2： | 1 |
| 16 | Regurgitation of bile acids from leaky bile ducts causes sclerosing cholangitis in Mdr2 ( Abcb4 ) knockout mice显示文摘 | Peter Fickert Andrea Fuchsbichler Martin Wagner Gernot Zollner Arthur Kaser Herbert Tilg Robert Krause Frank Lammert Cord Langner Kurt Zatloukal Hanns-Ulrich Marschall Helmut Denk Michael Trauner | 2004 | Gastroenterology2004，，1： | 1 |
| 17 | Spontaneous cholecysto - and hepatolithiasis in Mdr2 -/- mice: a model for low phospholipid -associated cholelithiasis 显示文摘 | Lammert F Wang DQ Hillebrandt S | 2004 | Hepatology2004，39，1： | 1 |
| 18 | Chromosomal organization of candidate genes involved in cholesterol gallstone formation: a murine gallstone map显示文摘 | Lammert F Carey MC Paigen B | 2001 | Gastroenterology2001，120，1： | 1 |
| 19 | Sterol carrier protein 2 participates in hypersecretion of biliary cholesterol during gallstone formation in genetically gallstone-susceptible mice显示文摘 | Fuchs M Lammert F Wang DQ | 1998 | Biochem J1998，336，1： | 1 |
| 20 | Chromosomal organization of candidate genes involved in cholesterol gallstone formation: A murine gallstone map显示文摘 | Frank Lammert Martin C. Carey Beverly Paigen | 2001 | Gastroenterology2001，，1： | 1 |
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