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| 1 | Transcription factor WRKY22 regulates canker susceptibility in sweet orange (Citrus sinensis Osbeck) by enhancing cell enlargement and CsLOB1 expression显示文摘Pathological hypertrophy(cell enlargement)plays an important role in the development of citrus canker,but its regulators are largely unknown.Although WRKY22 is known to be involved in pathogen-triggered immunity and positively regulates resistance to bacterial pathogens in Arabidopsis,rice and pepper,the CRISPR/Cas9-mediated partial knockout of CsWRKY22 improves resistance to Xanthomonas citri subsp.citri(Xcc)in Wanjincheng orange(Citrus sinensis Osbeck).Here,we demonstrate that CsWRKY22 is a nucleus-localized transcriptional activator.CsWRKY22-overexpressing plants exhibited dwarf phenotypes that had wrinkled and thickened leaves and were more sensitive to Xcc,whereas CsWRKY22-silenced plants showed no visible phenotype changes and were more resistant to Xcc.Microscopic observations revealed that the overexpression of CsWRKY22 increased cell size in the spongy mesophyll.Transcriptome analysis showed that cell growth-related pathways,such as the auxin and brassinosteroid hormonal signaling and cell wall organization and biogenesis pathways,were significantly upregulated upon CsWRKY22 overexpression.Interestingly,CsWRKY22 activated the expression of CsLOB1,which is a key gene regulating susceptibility to citrus canker.We further confirmed that CsWRKY22 bound directly to the W-boxes just upstream of the transcription start site of CsLOB1 in vivo and in vitro.We conclude that CsWRKY22 enhances susceptibility to citrus canker by promoting host hypertrophy and CsLOB1 expression.Thus,our study provides new insights into the mechanism regulating pathological hypertrophy and the function of WRKY22 in citrus. | Qin Long Meixia Du Junhong Long Yu Xie Jingyun Zhang Lanzhen Xu Yongrui He Qiang Li Shanchun Chen Xiuping Zou | 2021 | Horticulture Research2021，8，1： | 5 |
| 2 | CitGVD:a comprehensive database of citrus genomic variations显示文摘Citrus is one of the most important commercial fruit crops worldwide.With the vast genomic data currently available for citrus fruit,genetic relationships,and molecular markers can be assessed for the development of molecular breeding and genomic selection strategies.In this study,to permit the ease of access to these data,a web-based database,the citrus genomic variation database(CitGVD,http://citgvd.cric.cn/home)was developed as the first citrusspecific comprehensive database dedicated to genome-wide variations including single nucleotide polymorphisms(SNPs)and insertions/deletions(INDELs).The current version(V1.0.0)of CitGVD is an open-access resource centered on 1,493,258,964 high-quality genomic variations and 84 phenotypes of 346 organisms curated from in-house projects and public resources.CitGVD integrates closely related information on genomic variation annotations,related gene annotations,and details regarding the organisms,incorporating a variety of built-in tools for data accession and analysis.As an example,CitGWAS can be used for genome-wide association studies(GWASs)with SNPs and phenotypic data,while CitEVOL can be used for genetic structure analysis.These features make CitGVD a comprehensive web portal and bioinformatics platform for citrus-related studies.It also provides a model for analyzing genome-wide variations for a wide range of crop varieties. | Qiang Li Jingjing Qi Xiujuan Qin Wanfu Dou Tiangang Lei Anhua Hu Ruirui Jia Guojin Jiang Xiuping Zou Qin Long Lanzhen Xu Aihong Peng Lixiao Yao Shanchun Chen Yongrui He | 2020 | Horticulture Research2020，7，1： | 4 |
| 3 | Cloning and Expression Analysis of Citrus Genes Cs GH3.1 and Cs GH3.6Responding to Xanthomonas axonopodis pv. citri Infection显示文摘To study the functions of the early auxin-responsive genes Cs GH3.1 and Cs GH3.6 in citrus resistance against canker disease, we cloned Cs GH3.1and Cs GH3.6 in ‘Newhall' Navel Orange(Citrus sinensis Osbeck). They are 1 797 bp and 1 887 bp and encode 598 and 629 amino acids, respectively. In vitro mature leaves from susceptible ‘Newhall' and resistant Calamondin(C. madurensis) were inoculated by a Xanthomonas axonopodis pv. citri(Xac) bacterial suspension, and expression of Cs GH3.1 and Cs GH3.6 in the two varieties were analyzed using quantitative real-time PCR(q RT-PCR). ‘Newhall' leaves were treated with different hormones for 3 days, inoculated by Xac bacterial suspension, and then the symptoms in these leaves were investigated. We used q RT-PCR to analyze the effect of different hormones on Cs GH3.1 and Cs GH3.6 expression in ‘Newhall'leaves. The expression levels of both Cs GH3.1 and Cs GH3.6 were significantly induced by Xac in ‘Newhall' leaves, compared with levels in Calamondin leaves. 1-naphthy acetic acid(NAA) increased the hypertrophy of infection sites in ‘Newhall' leaves, while naphthyl-phthalamic acid(NPA)had no visible effect on lesion development. NAA hormone greatly improved expression of Cs GH3.1 in ‘Newhall', but not Cs GH3.6. These results indicate that the auxin primary-response gene Cs GH3.1 plays an important role in citrus susceptibility to Xac. | CHEN Min HE Yongrui XU Lanzhen PENG Aihong LEI Tiangang YAO Lixiao LI Qiang ZHOU Pengfei BAI Xiaojing DUAN Minjie JIANG Xueyou JIA Ruirui ZOU Xiuping CHEN Shanchun | 2016 | Horticultural Plant Journal2016，2，4： | 4 |
| 4 | The response of electron transport mediated by active NADPH dehydrogenase complexes to heat stress in the cyanobacterium Synechocystis 6803显示文摘The electron-transport machinery in photosynthetic membranes is known to be very sensitive to heat. In this study, the rate of electron transport (ETR) driven by photosystem I (PSI) and photosystem II (PSII) during heat stress in the wild-type Synechocystis sp. strain PCC 6803 (WT) and its ndh gene inactiva-tion mutants △ndhB (M55) and △ndhD1/ndhD2 (D1/D2) was simultaneously assessed by using the novel Dual-PAM-100 measuring system. The rate of electron transport driven by the photosystems (ETRPSs) in the WT, M55, and D1/D2 cells incubated at 30℃ and at 55℃ for 10 min was compared. Incubation at 55 ℃ for 10 min significantly inhibited PSII-driven ETR (ETRPSII) in the WT, M55 and D1/D2 cells, and the ex-tent of inhibition in both the M55 and D1/D2 cells was greater than that in the WT cells. Further, PSI-driven ETR (ETRPSI) was stimulated in both the WT and D1/D2 cells, and this rate was increased to a greater extent in the D1/D2 than in the WT cells. However, ETRPSI was considerably inhibited in the M55 cells. Analysis of the effect of heat stress on ETRPSs with regard to the alterations in the 2 active NDH-1 complexes in the WT, M55, and D1/D2 cells indicated that the active NDH-1 supercomplex and medi-umcomplex are essential for alleviating the heat-induced inhibition of ETRPSII and for accelerating the heat-induced stimulation of ETRPSI, respectively. Further, it is believed that these effects are most likely brought about by the electron transport mediated by each of these 2 active NDH-1 complexes. | MA WeiMin, WEI LanZhen & WANG QuanXi College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China | 2008 | Science China(Life Sciences)2008，51，12： | 4 |
| 5 | CsWAKL08,a pathogen-induced wall-associated receptor-like kinase in sweet orange,confers resistance to citrus bacterial canker via ROS control and JA signaling显示文摘Citrus bacterial canker(CBC)is a disease resulting from Xanthomonas citri subsp.citri(Xcc)infection and poses a grave threat to citrus production worldwide.Wall-associated receptor-like kinases(WAKLs)are proteins with a central role in resisting a range of fungal and bacterial diseases.The roles of WAKLs in the context of CBC resistance,however,remain unclear.Here,we explored the role of CsWAKL08,which confers resistance to CBC,and we additionally analyzed the molecular mechanisms of CsWAKL08-mediated CBC resistance.Based on systematic annotation and induced expression analysis of the CsWAKL family in Citrus sinensis,CsWAKL08 was identified as a candidate that can be upregulated by Xcc infection in the CBC-resistant variety.CsWAKL08 can also be induced by the phytohormones salicylic acid(SA)and methyl jasmonic acid(MeJA)and spans the plasma membrane.Overexpression of CsWAKL08 resulted in strong CBC resistance in transgenic sweet oranges,whereas silencing of CsWAKL08 resulted in susceptibility to CBC.The peroxidase(POD)and superoxide dismutase(SOD)activities were significantly enhanced in the CsWAKL08-overexpressing plants compared to the control plants,thereby mediating reactive oxygen species(ROS)homeostasis in the transgenic plants.Moreover,the JA levels and the expression of JA biosynthesis and JA responsive genes were substantially elevated in the CsWAKL08 overexpression plants relative to the controls upon Xcc infection.Based on these findings,we conclude that the wall-associated receptor-like kinase CsWAKL08 positively regulates CBC resistance through a mechanism involving ROS control and JA signaling.These results further highlight the importance of this kinase family in plant pathogen resistance. | Qiang Li Anhua Hu Jingjing Qi Wanfu Dou Xiujuan Qin Xiuping Zou Lanzhen Xu Shanchun Chen Yongrui He | 2020 | Horticulture Research2020，7，1： | 3 |
| 6 | PCR-based approaches for identification of multi-copy transgene integration sites in mouse genome显示文摘Generation of transgenic mice by DNA microinjection has become one of the most important technologies in studying gene function in vivo. Ran- dom integration of transgene often results in inser- tional mutation which may complicate phenotype analysis of transgenic mice and/or create a good opportunity to study the function of endogenous gene. However,the utilization of these potentially valuable resources is hampered due to lack of efficient ap- proaches for rapid identification of multi-copy trans- gene integration sites. Here we propose two PCR-based approaches to identifying trans- gene/genome junction sequences. The efficiency of these two strategies was tested in 9 independent transgenic mouse lines. Among them,the transgene in 8 mouse lines was clearly localized to certain chromosome regions. These rapid and efficient ap- proaches will greatly facilitate the study of insertional mutation due to transgene integration. | ZHAO Xudong DANG Suying LIANG Bin LEI Xia CHEN Zheng WANG Long YAN Lanzhen SUN Hantang FU Jiliang FEI Jian WANG Zhugang | 2006 | Chinese Science Bulletin2006，51，18： | 2 |
| 7 | Determination of Chinese honey adulterated with high fructose corn syrup by near infrared spectroscopy显示文摘 | Lanzhen Chen Xiaofeng Xue Zhihua Ye Jinghui Zhou Fang Chen Jing Zhao | 2010 | Food Chemistry2010，，4： | 1 |
| 8 | Sequences of (S)fuzzy integral functions显示文摘 | HA Minghu WANG Xizhao YANG Lanzhen | 2003 | Fuzzy Sets and Systems2003，138，： | 1 |
| 9 | Determination of Chinese honey adulterated with high fructose corn syrup by near infrared spectroscopy显示文摘 | Lanzhen Chen Xiaofeng Xue Zhihua Ye Jinghui Zhou Fang Chen Jing Zhao | 2010 | Food Chemistry2010，，4： | 1 |
| 10 | Biological properties of the chitosan-gelatin sponge wound dressing显示文摘 | Deng Chunmei He Lanzhen Zhao Ming | 2007 | Carbohydrate Polymers2007，69，： | 1 |
| 11 | Effect of cell wateramount on photosynthetic yield in the cyanobacterium Nos-toc flagelliforme and involvement of NADPH dehydrogen- ase-mediated cyclic electron transport显示文摘 | Lanzhen W Weimin M Quanxi W et al | 2009 | J Appl Phycol2009，21，： | 1 |
| 12 | Effi- cient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection 显示文摘 | Zou Xiuping Peng Aihong Xu Lanzhen | 2013 | Plant Cell Rep2013，32，10： | 1 |
| 13 | Mick to shu united salbutamol aerosel inhalation treatment curative effect observation and nursing pediatric asthma 显示文摘 | Zhuang Yan Yun Ye Liqin Chen Lanzhen | 2010 | Journal of general nursing2010，28，5： | 1 |
| 14 | Risk Factors and Pregnancy Outcome in Women with a History of Cesarean Section Complicated by Placenta Accreta显示文摘Objective:To explore the risk factors and pregnancy outcomes in women with a history of cesarean section complicated by placenta accreta(PA).Methods:This case-control study included clinical data from singleton mothers with a history of cesarean section in 11 public tertiary hospitals in seven provinces of China between January 2017 and December 2017.According to the intraoperative findings after delivery,the study population was divided into PA and non-PA groups.We compared the pregnancy outcomes between the two groups,used multivariate logistic regression to analyze the risk factors for placental accreta.Results:For this study we included 11,074 pregnant women with a history of cesarean section;and of these,869 cases were in the PA group and 10,205 cases were in the non-PA group.Compared with the non-PA group,the probability of postpartum hemorrhage(236/10,205,2.31%vs.283/869,32.57%),severe postpartum hemorrhage(89/10,205,0.87%vs.186/869,21.75%),diffuse intravascular coagulation(3/10,205,0.03%vs.4/869,0.46%),puerperal infection(33/10,205,0.32%vs.12/869,1.38%),intraoperative bladder injury(1/10,205,0.01%vs.16/869,1.84%),hysterectomy(130/10,205,1.27%vs.59/869,6.79%),and blood transfusion(328/10,205,3.21%vs.231/869,26.58%)was significantly increased in the PA group(P<0.05).At the same time,the neonatal birth weight 3250.00(2950.00–3520.00)g vs.2920.00(2530.00–3250.00)g),the probability of neonatal comorbidities(245/10,205,2.40%vs.61/869,7.02%),and the rate of neonatal intensive care unit admission(817/10,205,8.01%vs.210/869,24.17%)also increased significantly(P<0.05).Weight(odds ratio)(OR)=1.03,95%confidence interval(CI):1.01–1.05)),parity(OR=1.18,95%CI:1.03–1.34),number of miscarriages(OR=1.31,95%CI:1.17–1.47),number of previous cesarean sections(OR=2.57,95%CI:2.02–3.26),history of premature rupture of membrane(OR=1.61,95%CI:1.32–1.96),previous cesarean-section transverse incisions(OR=1.38,95%CI:1.12–1.69),history of placenta previa(OR=2.44,95%CI:1.50–3.96),and the combination of prenatal hemorrhage(OR=9.95,95%CI:8.42–11.75)and placenta previa(OR=91.74,95%CI:74.11–113.56)were all independent risk factors for PA.Conclusion:There was an increased risk of adverse outcomes in pregnancies complicated by PA in women with a history of cesarean section,and this required close clinical attention.Weight before pregnancy,parity,number of miscarriages,number of previous cesarean sections,history of premature rupture of membranes,past transverse incisions in cesarean sections,a history of placenta previa,prenatal hemorrhage,and placenta previa were independent risk factors for pregnancies complicated with PA in women with a history of cesarean section.These independent risk factors showed a high value in predicting the risk for placentab accreta in pregnancies of women with a history of cesarean section. | Yingyu Liang Lizi Zhang Shilei Bi Jingsi Chen Shanshan Zeng Lijun Huang Yulian Li Minshan Huang Hu Tan Jinping Jia Suiwen Wen Zhijian Wang Yinli Cao Shaoshuai Wang Xiaoyan Xu Ling Feng Xianlan Zhao Yangyu Zhao Qiying Zhu Hongbo Qi Lanzhen Zhang Hongtian Li Lili Du Dunjin Chen | 2022 | Maternal-Fetal Medicine2022，4，3： | 1 |
| 15 | Determination of organochlorine pesticides in propolis by gas chromatography-electron capture detection using double column series solid-phase extraction显示文摘 | Fang CHEN Lanzhen CHEN Qiang WANG | 2009 | Anal Bioanal Chem2009，393，： | 1 |
| 16 | On the fundamental convergence in the (C) mean in problems of information fusion显示文摘 | PEDRYCZ W YANG Lanzhen HA Minghu | 2009 | J Math Anal Appl2009，358，： | 1 |
| 17 | Determination of organochlorine pesticides in propolis by gas chromatography–electron capture detection using double column series solid-phase extraction显示文摘 | Fang Chen Lanzhen Chen Qiang Wang Jinhui Zhou Xiaofeng Xue Jing Zhao | 2009 | Analytical and Bioanalytical Chemistry2009，，3： | 1 |
| 18 | Preparation of the photosensitive copper complex and CuO film pattern显示文摘 | Zhang Huangli Zhao Gaoyang Xu Lanzhen | | 0，，： | 1 |
| 19 | Analysis of coenzyme Q10 in bee pollen using online cleanup by accelerated solvent extraction and high performance liquid chromatography显示文摘 | XUE Xiaofeng ZHAO Jing CHEN Lanzhen | 2012 | Food Chemistry2012，133，： | 1 |
| 20 | EMMPRIN基因增强CT26小鼠结肠癌细胞的转移潜能(英文)显示文摘Objective: Colon cancer metastasis is the key in fertility rate of colon cancer. Many recent results about metastasis research indicated that EMMPRIN played an important role in cancer metastasis. So, we designed this experiment to investigate whether EMMPRIN can enhance the metastatic ability of murine colon adenocarcinoma cell, CT26. Methods: EMMPRIN was over expressed in CT26 cells through transfecting pCMV-HA2-EMMPRIN into the CT26 cells. Invasion assay, wound migration assay and adhesion assay were utilized to analyze the metastasis of CT26 cells in vitro after EMMPRIN over expression. Results: After EMMPRIN over expression, invasion assay showed that invasive cells were 103.33 + 8.49 in EMMPRIN group and 48.67 + 5.3 in control group (P < 0.001). Migration assay showed that migrating cells were 40.67 + 2.49 in EMMPRIN group and 18.33 + 2.05 in control group (P < 0.001). CCK-8 absorbance value in adhesion assay were 3.33 + 0.17 in EMMPRIN group and 2.10 + 0.22 in control group (P < 0.001). Conclusion: Over expression of EMMPRIN could enhance the CT26 cell capacity of invasion and migration, and inhibit CT26 cell capacity of adhesion remarkably. The results suggest that EMMPRIN may be involved in cancer metastasis and play an important role in promotion of cancer metastasis. | Ling Dai Xiaoling Tian Ning Tan Haibin Wen Lanzhen Huang | 2011 | The Chinese-German Journal of Clinical Oncology2011，10，11： | 0 |
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