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| 1 | Effects of donor cells on in vitro development of cloned bovine embryos显示文摘The donor cells from different individuals and with different foreign genes introduced were investigated to determine their effects on the efficiency of somatic cell nuclear transfer (SCNT). The bovine ear fibroblast from different individuals was isolated, cultured, and then transfected with foreign genes to establish the stable cell lines, which were used as donor cells for nuclear transfer. The oocytes were obtained through ovum pick up operation. After in vitro maturation, the M II phase oocytes were selected as receptors for nuclear transfer. The reconstructed embryos were cultured in vitro and observed at 2 h, 48 h, and 7 days after transfer to assess the rate of fusion using cleaved and blastocyst as the parameters of SCNT efficiency. The donor cells from different individuals (04036, 06081, 06088, and 06129) had no obvious effect on the fusion and cleaved rate, whereas there was significant difference in the blastocyst rate (P<0.05), and the rate was 62.3%, 37.0%, 35.1%, and 15.6%, respectively. There was no significant difference among the rate of fusion, cleaved and blastocyst in donor cells with different foreign genes (P>0.05). It was concluded that the genetic background of the donor cells could affect the effi- ciency of SCNT, while the introduction of foreign genes into the donor cells had no obvious effect on the efficiency. This study provides useful information for the SCNT and would benefit in promoting the efficiency. | Jing Fu Pengfei Guan Leiwen Zhao Hua Li Shuzhen Huang Fanyi Zeng Yitao Zeng | 2008 | Journal of Genetics and Genomics2008，35，5： | 6 |
| 2 | Supplement to the published paper'Theory-guided interventions for Chinese patients to adapt to heart failure:A quasi-experimental study'显示文摘We appreciate the question raised in a discussion with a reader and the editor about an instrument for outcome measure of the quality of life among people with chronic heart failure[1].We recognized that we should have described more details about how to convert the score of the Minnesota Living with Heart Failure Questionnaire(MLHFQ).Lack of scoring details might lead to some confusion to understand the results of this study.We thank the International Journal of Nursing Sciences for the opportunity to provide further information to clarify the process of data analysis and research findings. | Xiyi Wang Leiwen Tang Doris Howell Qi Zhang Ruolin Qiu Hui Zhang Zhihong Ye | 2021 | International Journal of Nursing Sciences2021，8，2： | 3 |
| 3 | Quantitative analysis of elements (C,N,O,Al,Si and Fe) in polyamide with wavelength dispersive X-ray fluorescence spectrometry显示文摘A wavelength dispersive X-ray fluorescence (WD-XRF) spectrometry combined with calibration curve method was established for simultaneously analyzing low-Z elements (C,N,O) and Al,Si,Fe in polyamide.To investigate the origin of plastic material causing deposition and blocking in instrument engines and pipelines,polyamide 6 (PA 6,an engineering plastic) was chosen as the study object on account of its common use in industry.The sample preparation with pressed powder disk has been developed for determination of six elements in PA 6.Pure Cu metal was used as the matrix and PA 6 was regarded as standard sample for C,N,O elements.PA 6 particles were firstly smashed to uniform powder in liquid nitrogen,and then mixed with inorganic standard powders (Fe2O3,Al2O3,SiO2,and Na 2 SiO 3).The mixture was ground to obtain homogeneous calibration materials for WD-XRF analysis.The quantitative property of the calibration curve method for each element was reliable.The limits of detection (S/N≤3) of C,N,O,Al,Si and Fe using WD-XRF were 249,120,101,6.2,3.3,and 1.8 μg/g,respectively.To confirm the accuracy of the proposed WD-XRF calibration curve method,inductively coupled plasma optical emission spectroscopy (ICP-OES) detection for Al,Si,Fe and elemental analyzer (EA) analysis for C,N,O were utilized.A good correlation of the WD-XRF results with the measurements of ICP-OES and EA was observed. | LAI MeiShuang XIANG LeiWen LIN Jin-Ming LI Hai-Fang | 2013 | Science China Chemistry2013，56，8： | 3 |
| 4 | Housing Inequality in Transitional Beijing显示文摘 | Huang Youqin and Jiang Leiwen | 2009 | International Journal of Urban and Regional Research2009，33，4： | 1 |
| 5 | Urban and rural energy use and carbon dioxide emissions in Asia显示文摘 | Volker Krey Brian C. O’Neill Bas van Ruijven Vaibhav Chaturvedi Vassilis Daioglou Jiyong Eom Leiwen Jiang Yu Nagai Shonali Pachauri Xiaolin Ren | 2012 | Energy Economics2012，，： | 1 |
| 6 | Population aging and future carbon emissions in the United States显示文摘 | Michael Dalton Brian O’Neill Alexia Prskawetz Leiwen Jiang John Pitkin | 2006 | Energy Economics2006，，2： | 1 |
| 7 | The household energy transition in India and China显示文摘 | Shonali Pachauri Leiwen Jiang | 2008 | Energy Policy2008，36，11： | 1 |
| 8 | The Household Energy Transition in India and China 显示文摘 | Shonali Pachauri Leiwen Jiang | 2008 | Energy Policy2008，36，： | 1 |
| 9 | The Energy Transition in Rural China显示文摘 | Jiang Leiwen Brian Neill C O | 2004 | International Journal of Global Energy Issues2004，21，12： | 1 |
| 10 | Living Conditions of the Floating Population in Urban China显示文摘 | Jiang Leiwen | 2006 | Housing Studies2006，，5： | 1 |
| 11 | Living Conditions of the Floating Population in Urban China显示文摘 | Leiwen Jiang | 2006 | Housing Studies2006，，5： | 1 |
| 12 | iASPPsv antagonizes apoptosis induced by chemotherapeutic agents in MCF-7 cells and mouse thymocytes显示文摘 | Lin Wang Haiyan Xing Zheng Tian Leiwen Peng Yan Li Kejing Tang Qing Rao Min Wang Jianxiang Wang | 2012 | Biochemical and Biophysical Research Communications2012，，3： | 1 |
| 13 | The household energy transition in India and China显示文摘 | Shonali Pachauri Leiwen Jiang | 2008 | Energy Policy2008，，11： | 1 |
| 14 | Correlates of Sexual Lifestyles Among Older Adults Living With HIV in China: Findings From the Sexual Well-being (SWELL) Study显示文摘Sexual lifestyles are closely related to overall human health and well-being.Few studies have focused on sexual lifestyles among older adults living with HIV(OALHIV),especially in low-and middle-income countries.This study is a part of the sexual well-being among older adults in China(SWELL)study,which is a multicenter cross-sectional study focused on sexual health among older adults aged 50 years and older.Participants were 680 OALHIV(≥50 years old)from the SWELL study conducted from June 2020 to December 2022.Data were collected through one-on-one interviews.We used logistic regression to assess the correlates of sexual activity and sexual satis-faction.Among all participants,37.1%were sexually active.Being older,male,in a steady relationship and employed were associated with being sexually active.The prevalence of sexual satisfaction was 69.8%among sexually active OALHIV.Being homosexual and reporting a better general health status were associated with sexual satisfaction.The OALHIV who have depressive symptoms were less likely to report sexual satisfaction.To support holistic healthy aging among OALHIV,it is important for healthcare providers to be educated about the importance of enquiring about sexual activity,satisfaction and problems and addressing concerns while conveying sex-positive attitudes during clinical reviews,as these are still critical aspects of their health and well-being. | Bingyi Wang Xin Peng Leiwen Fu Xinyi Li Tongxin Shi Bowen Liang Tian Tian Thomas Fitzpatrick Zhen Lu Weijie Zhang JunyeBian Xin Xiao YingWang Yunlong Ao Lin Ouyang YongCai Maohe Yu Guohui Wu Linghua Li Xiaojun Meng Huachun Zou | 2023 | Infectious Microbes & Diseases2023，5，3： | 1 |
| 15 | Population aging and future carbon emissions in the United States 显示文摘 | Michael Dalton Brian O'Neill Alexia Prskawetz Leiwen Jiang John Pitkin | 2008 | Energy Economics2008，，30： | 1 |
| 16 | Characterization of the human GARP (Golgi associated retrograde protein) complex显示文摘 | LEIWEN H MEINHOLD-HEERLEIN I OLIVEIRA V | 2005 | Exp Cell Res2005，306，： | 1 |
| 17 | Future Trend of Family Households and Elderly Living Arrangement in China 显示文摘 | Zeng Yi Zhenglian Wang Leiwen Jang Danan Gu | 2008 | Genus2008，64，12： | 1 |
| 18 | The household energy transition in India and China显示文摘 | Shonali Pachauri Leiwen Jiang | | 0，，11： | 1 |
| 19 | 显示文摘 | WANGJian-Hua(王建华) LEIWen(雷文) WANGYuan-Liang(王远壳) | | HuaxueTongbao0，，： | 1 |
| 20 | Ground move- ments caused by deep underground mining in Guan--Zhuang iron mine 显示文摘 | Wen--Xiu Li LeiWen Xiao--Min Liu | 2010 | International Journal of Applied Earth Ob- servation and Geoinformation2010，12，： | 1 |
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