| 1 | Transcriptomic analysis reveals the effect of the exopolysaccharide of Psychrobacter sp.B-3 on gene expression in RAW264.7 macrophage cells显示文摘B-3 exopolysaccharide is extracted from the Antarctic psychrophilic bacterium Psychrobacter sp. B-3. We have previously shown that it activates macrophages and affects their immunoregulatory activities. To determine what genes are affected during this process, we detected the genes differentially expressed in cells of RAW264.7 macrophages treated with B-3 exopolysaccharide by transcriptomic analysis. B-3 exopolysaccharide treatment caused differential expression of 420 genes, of which 178 were up-regulated and 242 were down-regulated. These genes were shown to be involved in many aspects of cell function, mainly metabolism and immunity. Genes were enriched in multiple immune-related pathways, and the most significantly enriched genes were involved in antigen processing and presentation pathways. The pathway in which differentially expressed genes were the most significantly enriched was the metabolic pathway; specifically, the expression of many metabolic enzyme genes was altered by B-3 exopolysaccharide treatment. Additionally, the genes involved in metabolisms of amino acids, carbohydrates, lipids and nucleotides, varied to certain degrees. B-3 exopolysaccharide, therefore, appears to directly affect the immune function of RAW264.7 macrophages as an immunostimulant, or to indirectly change intracellular metabolism. This is the first study to determine the effect of an Antarctic psychrophilic bacterial exopolysaccharide on RAW264.7 macrophages. Our findings provide an important reference for research into the regulation of macrophage immune function by different polysaccharides. | ZHANG Pingping LI Jiang YU Leiye WEI Jingfang XU Tong SUN Guojie | 2018 | Acta Oceanologica Sinica2018，37，8： | 1 |
| 2 | Molecular basis of Spns2-facilitated sphingosine-1-phosphate transport显示文摘Dear Editor,As one of the critical sphingolipid metabolites in eukaryotes,sphingosine-1-phosphate(S1P)acts as a bioactive lipid mediator in the immune and vascular systems.S1P prompts its physiological roles through two mechanisms,binding to its intracellular targets or extracellular secretion.Intracellular S1P promotes cellular proliferation,whereas plasma S1P facilitates immune cell trafficking,regulates angiogenesis,and helps to maintain vascular integrity.1 Due to the amphipathic property,S1P cannot diffuse freely but has to be transported across the cell membrane through active transport.1 In the past two decades,several S1P transporters have been identified,including two major facilitator superfamily(MFS)members:Spinster homolog 2(Spns2)and Mfsd2b,and some ATP-binding cassette family transporters.Among these transporters,Spns2 is the first identified and the most extensively studied.2 Here,we reported two cryo-electron microscopy(EM)structures of human Spns2 in inward-open conformations bound to S1P or inhibitor 16d. | Bin Pang Leiye Yu Tong Li Haizhan Jiao Xiaomei Wu Jinxin Wang Ruiping He Yurou Zhang Juan Wang Hongli Hu Wei Dai Li Chen Ruobing Ren | 2024 | Cell Research2024，34，2： | 0 |
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