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| 1 | Biotinylated dextran amine anterograde tracing of the canine corticospinal tract显示文摘In this study, biotinylated dextran amine (BDA) was microinjected into the left cortical motor area of the canine brain. Fluorescence microscopy results showed that a large amount of BDA-labeled pyramidal cells were visible in the left cortical motor area after injection. In the left medulla oblongata, the BDA-labeled corticospinal tract was evenly distributed, with green fluorescence that had a clear boundary with the surrounding tissue. The BDA-positive corticospinal tract entered into the right lateral funiculus of the spinal cord and descended into the posterior part of the right lateral funiculus, close to the posterior horn, from cervical to sacral segments. There was a small amount of green fluorescence in the sacral segment. The distribution of BDA labeling in the canine central nervous system was consistent with the course of the corticospinal tract. Fluorescence labeling for BDA gradually diminished with time after injection. Our findings indicate that the BDA anterograde tracing technique can be used to visualize the localization and trajectory of the corticospinal tract in the canine central nervous system. | Xiao Han Guangming Lv Huiqun Wu Dafeng Ji Zhou Sun Yaofu Li Lemin Tang | 2012 | Neural Regeneration Research2012，7，11： | 2 |
| 2 | Magnetic resonance morphometry of the loss of gray matter volume in Parkinson's disease patients显示文摘Voxel-based morphometry can be used to quantitatively compare structural differences and functional changes of gray matter in subjects.In the present study,we compared gray matter images of 32 patients with Parkinson's disease and 25 healthy controls using voxel-based morphometry based on 3.0 T high-field magnetic resonance T1-weighted imaging and clinical neurological scale scores.Results showed that the scores in Mini-Mental State Examination and Montreal Cognitive Assessment were lower in patients compared with controls.In particular,the scores of visuospatial/executive function items in Montreal Cognitive Assessment were significantly reduced,but mean scores of non-motor symptoms significantly increased,in patients with Parkinson's disease.In addition,gray matter volume was significantly diminished in Parkinson's disease patients compared with normal controls,including bilateral temporal lobe,bilateral occipital lobe,bilateral parietal lobe,bilateral frontal lobe,bilateral insular lobe,bilateral parahippocampal gyrus,bilateral amygdale,right uncus,and right posterior lobe of the cerebellum.These findings indicate that voxel-based morphometry can accurately and quantitatively assess the loss of gray matter volume in patients with Parkinson's disease,and provide essential neuroimaging evidence for multisystem pathological mechanisms involved in Parkinson's disease. | Jianguo Xia Juan Wang Weizhong Tian Hongbin Ding Qilin Wei Huanxin Huang Jun Wang Jinli Zhao Hongmei Gu Lemin Tang | 2013 | Neural Regeneration Research2013，8，27： | 1 |
| 3 | Improving Thermal and Flame Retardant Properties of Epoxy Resin with Organic NiFe-Layered Double Hydroxide-Carbon Nanotubes Hybrids显示文摘在环氧基树脂树脂(EP ) 改进碳 nanotubes (CNT ) 和分层的双氢氧化物(LDH ) 的火焰 retardancy 的分散,器官的镍铁分层的双氢氧化物(ONiFe-LDH-CNTs ) 混血儿通过一起沉淀被装配。这些混血儿进一步被用作增强 filler in EP。与 ONiFe-LDH 和 CNT 的不同比率包含 ONiFe-LDH-CNTs 的 4 wt% 的 EP/ONiFe-LDH-CNTs nanocomposites 被治好的超声的分散和节目温度准备。获得的混血儿的结构和形态学被不同技术描绘。在 EP 矩阵的 nanofillers 的分散被传播电子显微镜学(TEM ) 观察。结果揭示了 exfoliated 的共存并且设置在聚合物矩阵的 ONiFe-LDH- CNT。有 EP 矩阵的上述 nanofillers 的强壮的联合提供了一有效热并且为 nanocomposites 的火焰 retardant 改进。它证明 EP/ONiFe-LDH-CNTs nanocomposites 与 EP 相比展出了优异火焰 retardant 和热性质。如此的改进热性质能被归因于更好同类的分散,更强壮的界面的相互作用, ONiFe-LDH 的优秀 charring 表演和在 ONiFe-LDH 和 CNT 之间的 synergistic 效果。 | Qinghong Kong Ting Wu Yingqi Tang Lemin Xiong Hong Liu Junhao Zhang Ruihua Guo Feng Zhang | 2017 | Chinese Journal of Chemistry2017，35，12： | 1 |
| 4 | Three-dimensional reconstruction of human spinal cord based on histological serial sections显示文摘BACKGROUND: It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution and contrast in macrosection images. OBJECTIVE: To explore a semi-automatic computerized three-dimensional (3D) reconstruction of human spinal cord based on histological serial sections, in order to solve issues such as low contrast. DESIGN, TIME AND SETTING: An experimental study combining serial section techniques and 3D reconstruction, performed in the laboratory of Human Anatomy and Histoembryology at the Medical School of Nantong University during January to April 2008. SETTING: Department of Anatomy, Institute of Neurobiology, Jiangsu Province Key Laboratory of Neural Regeneration, Laboratory of Image Engineering. MATERIALS: A human lumbar spinal cord segment from fresh autopsy material of an adult male. METHODS: After 4% paraformaldehyde fixation for three days, serial sections of the lumbar spinal cord were cut on a Leica cryostat and mounted on slides in sequence, with eight sections aligned separately on each slide. All sections were stained with Luxol Fast Blue to reveal myelin sheaths. After gradient dehydration and clearing, the stained slides were coverslipped. Sections were observed and images recorded under a light microscope using a digital camera. Six images were acquired at ×25 magnification and automatically stitched into a complete section image. After all serial images were obtained, 96 complete serial images of the human lumbar cord segment were automatically processed with 'Curves', 'Autocontrast', ' Gray scale 8 bit', 'Invert', 'Image resize to 50%' steps using Photoshop 7.0 software. All images were added in order into 3D-DOCTOR 4.0 software as a stack, where serial images were automatically realigned with neighboring images and semi-automatically segmented for white matter and gray matter. Finally, simple surface and volume reconstruction were completed on a personal computer. The reconstructed human lumbar spinal cord segment was interactively observed, cut, and measured. MAIN OUTCOME MEASURES: The reconstructed human lumbar spinal cord segment. RESULTS: Compared with serial images obtained from other image modalities, such as CT, MRI, and mac- rosections from The Visual Human Project database, the Luxol Fast Blue stained histological serial section images exhibited higher resolution and contrast between gray and white matter. Image processing and 3D reconstruction steps were semi-automatically performed with related software. The 3D reconstructed human lumbar cord segment were observed, cut, and measured on a PC. CONCLUSION: A semi-automatically computerized method, based on histological serial sections, is an effective way to 3D-reconstruct the human spinal cord. | Guangming Lü Huiqun Wu Lemin Tang Xiao Han Dafeng Ji Xiaosong Gu | 2008 | Neural Regeneration Research2008，3，7： | 1 |
| 5 | Genetic variants in fas signaling pathway genes and risk of gastric cancer显示文摘 | Paula L. Hyland Shih‐Wen Lin Nan Hu Han Zhang Lemin Wang Hua Su Chaoyu Wang Ti Ding Ze‐Zhong Tang Jin‐Hu Fan You‐Lin Qiao Xiaoqin Xiong William Wheeler Carol Giffen Kai Yu Jeff Yuenger Laurie Burdett Zhaoming Wang Stephen J. Chanock Margaret A. Tucker San | 2014 | Int J Cancer2014，，4： | 1 |
| 6 | Fluoro-ruby retrograde tracing and three-dimensional visualization of the corticospinal tract in the guinea pig显示文摘Fluoro-ruby was injected into the posterior funiculus of the spinal cord in the cervical(C5-T2) and lumbar(L3-6) segments of adult guinea pigs.The spinal cord was cut into serial frozen sections.The Fluoro-ruby labeling was clearly delineated from the surrounding structure.The labeling traversed the cervical,thoracic and lumbar segments,and was located on the ventral portion of the posterior funiculus on the injected side,proximal to the intermediate zone of the dorsal gray matter.The fluorescence area narrowed rostro-caudally.The spinal cord,spinal cord gray matter and corticospinal tract were reconstructed using 3D-DOCTOR 4.0 software,resulting in a robust three-dimensional profile.Using functionality provided by the reconstruction software,free multi-angle observation and sectioning could be conducted on the spinal cord and corticospinal tract.Our experimental findings indicate that the Fluoro-ruby retrograde fluorescent tracing technique can accurately display the anatomical location of corticospinal tract in the guinea pig and that three-dimensional reconstruction software can be used to provide a three-dimensional image of the corticospinal tract. | Xiao Han Lulian Xu Yue Wu Hua Xun Jinxiu Pan Yingying Huang Dafeng Ji Huiqun Wu Guangming Lv Lemin Tang | 2012 | Neural Regeneration Research2012，7，5： | 0 |
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