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| 1 | Experimental and clinic-opathologic study on the relationship between transcription factor Egr-1 and esophageal carcinoma显示文摘AIM To observe the growth suppression effect of exogenous introduction of early growth response gene-1 (Egr-1 gene) on esophageal carcinoma tissue as well as on esophageal carcinoma cell line Eca109 and to explore the potential application of Egr-1 gene in gene therapy of tumor.METHODS Eukaryotic expression vector of PCMV-Egr-1 plasmid was introduced into Eca109 cell line which expressed no Egr-1 protein originally with lipofectamine transfection method. The introduction and expression of PCMV-Egr-1 plasmid into Eca109 cell line was confirmed by G418 selection culture, PCR amplification of neogene contained in the vector, Western blot analysis and immunocytochemical analysis. The cell growth curve,soft agar colony formation rate and tumorigenicity in SClD mice were examined to demonstrate the growth suppression effect of exogenous Egr-1 gene on Eca109 cell line. The Egr-1 mRNA and Egr-1 protein were also detected in 50 surgical specimens of esophageal carcinoma by in situ hybridization and immunohistochemistry.RESULTS Exogenous Egr-1 gene was introduced successfully into Eca109 cell line and expressed Egr-1 protein stably. The transfected Eca109 cell line grew more slowly than control Eca109 as shown by cell growth curves, the soft agar colony formation rate (4.0% vs 6.9%, P<0.01) and the average growth rate of tumor in SCID mice (35.5 ± 7.6 vs 65.8 ± 7.6, P<0.05). The expression level of Egr-1 mRNA and protein significantly increased in dysplastic epithelia adjacent to cancer rather than in cancer tissues (65.8% vs 20.0% by ISH and 57.9% vs 14.0% by IHC, P<0.01).CONCLUSION Exogenous Egr-1 gene shows the strong effect of growth inhibition in Eca109 cell line. Egr-1 in the cancer tissue shows down-regulated expression that supports the inhibited function of Egr-1 in cancer growth and suggests Egr-1 may have an important role in gene therapy of esophageal carcinoma. | Ming-Yao Wu Mao-Huai Chen Ying-Rui Liang Guo-Zhao Meng Huan-Xing Yang Chu-Xiang Zhuang Department of Pathology,Shantou University Medical College,Shantou 515031,Guangdong Province,China Supported by the National Natural Science Foundation of China,No.39670298. | 2001 | World Journal of Gastroenterology2001，7，4： | 20 |
| 2 | CONTINUOUS L-DOMAINS显示文摘Two characterization theorems of continuous L-domain are given. Then a problem raisedby J. D. Lawson and M. Mislove is solved. | LIANG JIHUA LIU YINGMING(Institute of Mathematics, Sichuan University, Chengdu 610064, China.)(Project supported by the National Natural Science Foundation of China.) | 1996 | Chinese Annals of Mathematics,Series B1996，17，3： | 8 |
| 3 | Retrieving crop leaf area index by assimilation of MODIS data into a crop growth model显示文摘Leaf area index (LAI) is an important parameter in monitoring crop growth. One of the methods for retrieving LAI from remotely sensed observations is through inversion of canopy reflectance models. Many model inversion methods fail to account for variable LAI values at different crop growth stages. In this research, we use the crop growth model to describe the LAI changes with crop growth, and consider a priori LAI values at different crop growth stages as constraint information. The key approach of this research is to assimilate multiple canopy reflectance values observed at different growth stages and a priori LAI values into a coupled crop growth and radiative transfer model sequentially using a variational data assimilation algorithm. Adjoint method is used to minimize the cost function. Any other information source can be easily incorporated into the inversion cost function. The validation results show that the time series of MODIS canopy reflectance can greatly reduce the uncertainty of the inverted LAI values. Compared with MODIS LAI product at Changping and Shunyi Counties of Beijing, this method has significantly improved the estimated LAI temporal profile. | WANG DongWei1,2,3, WANG JinDi1,2 & LIANG ShunLin4 1 State Key Laboratory of Remote Sensing Science, Jointly Sponsored by Beijing Normal University and Institute of Remote Sensing Applications, CAS, Beijing 100875, China 2 School of Geography, Beijing Key Laboratory of Environmental Remote Sensing and Digital City, Beijing Normal University, Beijing 100875, China 3 Haihe River Water Conservancy Commission, Tianjin 300170, China 4 Department of Geography, University of Maryland, College Park, MD 20742, USA | 2010 | Science China Earth Sciences2010，53，5： | 8 |
| 4 | Remote sensing and geographic information systems in the spatial temporal dynamics modeling of infectious diseases显示文摘Similar to species immigration or exotic species invasion,infectious disease transmis-sion is strengthened due to the globalization of human activities. Using schistosomiasis as an exam-ple,we propose a conceptual model simulating the spatio-temporal dynamics of infectious diseases. We base the model on the knowledge of the interrelationship among the source,media,and the hosts of the disease. With the endemics data of schistosomiasis in Xichang,China,we demonstrate that the conceptual model is feasible; we introduce how remote sensing and geographic information systems techniques can be used in support of spatio-temporal modeling; we compare the different effects caused to the entire population when selecting different groups of people for schistosomiasis control. Our work illustrates the importance of such a modeling tool in supporting spatial decisions. Our mod-eling method can be directly applied to such infectious diseases as the plague,lyme disease,and hemorrhagic fever with renal syndrome. The application of remote sensing and geographic informa-tion systems can shed light on the modeling of other infectious disease and invasive species studies. | GONG Peng 1,XU Bing 2 & LIANG Song 1、3 1. State Key Laboratory of Remote Sensing Science,Jointly Sponsored by the Institute of Remote Sensing Applications,Chi-nese Academy of Sciences and Beijing Normal University,Box 9718,Beijing 100101,China 2. Department of Geography,University of Utah,Salt Lake City,UT 84112,USA 3. School of Public Health,University of California,Berkeley,CA 94720,USA | 2006 | Science China(Life Sciences)2006，49，6： | 5 |
| 5 | Loose architecture of multi-level massive geospatial data based on virtual quadtree显示文摘This paper proposed a virtual quadtree (VQT) based loose architecture of multi-level massive geospatial data for integrating massive geospatial data dispersed in the departments of different hierarchies in the same sector into a unified GIS (Geographic Information System) platform. By virtualizing the nodes of the quad-tree,the VQT separates the structure of data organization from data storage,and screens the difference between the data storage in local computer and in the re-mote computers in network environment. And by mounting,VQT easily integrates the data from the remote computers into the local VQT so as to implement seam-less integration of distributed multi-level massive geospatial data. Based on that mode,the paper built an application system with geospatial data over 1200 GB distributed in 12 servers deployed in 12 cities. The experiment showed that all data can be seamlessly rapidly traveled and performed zooming in and zooming out smoothly. | YANG ChongJun,WU Sheng,REN YingChao,FU Li,ZHANG FuQing,WANG Gang,TAN Jian,LIU DongLin,MA ChaoJi & LIANG Li State Key Laboratory of Remote Sensing Science,Jointly Sponsored by the Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University,Beijing 100101,China | 2008 | Science China(Technological Sciences)2008，51，S1： | 4 |
| 6 | A Study of the Next Generation Intelligent Networks Interworking with IP Networks显示文摘In the past three years, the author has participated in several projects involved in Intelligent Network (IN). In 1997, we started our research on the project 'The Integration of IN and B ISDN' supported by National Science Foundation. Since 1999, the author has engaged in the project 'the Next Generation IN Based Service Platform Interworking with IP Networks' supported by the National 863 Project. While working on the project, the author made a deep research on the architecture and the key technology of the next generation IN defined by ITU T IN CS3 and CS4. In the following parts. 1. I conclude the research work and the innovation in my dissertation. The early research on the interworking of IN and Internet, despite the shortage of materials, the interworking architecture by means of IAF is analyzed. Some key techniques like triggering intelligent service and reporting dynamic IP address are solved. Some new SIBs and INAPs are added, and the dial up software of users is enhanced, too. The incoming call notification service is designed. What we have done paves the way for the future research. 2. The gateway based architecture for the interworking of IN and Internet is described. Considering the research results from ITU, PINT work group and TIPHON work group, we emphasize that the interworking scenario through MG,SCG and C/BG is promising. Based on the CIN software developed by ourselves, we provide the scenario to enhance the function entities and signaling protocol. We address the function of entities and the interaction of signaling with an example like ICW service. 3. With the introduction of mobile agent technology, the author proposes the distributed IN which can be integrated with IP networks. According to the drawbacks of the centralized IN, we address the architecture and conceptual model of distributed IN to support advanced service in IP Telephony. We try to illustrate the role and relationship between function entities. We also present a smooth evolution scenario from today’s networks. 4. A novel service control software based on mobile agent technology is proposed. With the adiquately understanding of CIN 02 SCP, we present the OO based framework of MA SCP. And we explore how our new SCP works. We prove that our solution can work efficiently by a service called VPN in IP Telephony. 5. The concept, architecture and related research of active networks are introduced. We take the ticket order service as an example, give the simulation results and prove that active networks can improve the performance of distributed applications. We also compare active networks with mobile agent based IN. We highlight that the two techniques can complement each other and borrow lessons from the other one. 6. The architecture of Broadband IN is discussed. From the angles of architecture, access mode and software structure, a novel B IP that can support multimedia services such as VoD and B VPN is given. Moreover, B IP is introduced into VoD service. To improve the performance of VoD, we present a novel replacement algorithm with the multi layer decision with multi factors. | CHEN Jun directed by CHEN Jun liang | 2001 | The Journal of China Universities of Posts and Telecommunications2001，8，4： | 2 |
| 7 | Multicomponent spectral correlative chromatography applied to complex herbal medicines显示文摘 | Hu Y Liang YZ Li BY | | 0，，26： | 1 |
| 8 | Prevalence of epilepsy in the People’s Republic of China:A systematic review显示文摘 | Gu L Liang BY Chen Q | 2013 | Epilepsy Res2013，105，12： | 1 |
| 9 | Trends in prevalence,awareness,treatment,and control of diabetes mellitus in mainland china from1979to 2012显示文摘 | Li MZ Su L Liang BY | 2013 | Int J Endocrinol2013，2013，75： | 1 |
| 10 | Nucleophosmin/B23 regulates transcriptional activation of E2F1 via modulating the promoter binding of NF-kappaB, E2F1 and pRB 显示文摘 | Lin CY Liang YC Yung BY | 2006 | Cell Signal2006，18，11： | 1 |
| 11 | Evaluation of gas chromatography /mass spectrometry in conjunction with chemometric resolution foridentification of nitrogen compounds in crude oiI显示文摘 | Li BY Liang YZ Hu Y | 2003 | Talanta2003，61，6： | 1 |
| 12 | Calpain activation promotes BACE1 expression, amyloid preeursor protein processing, and arny- loid plaque formation in a transgenie mouse model of Alzheimer dis- ease 显示文摘 | Liang B Duan BY Zhou XP | 2010 | J Biol Chem2010，285，27： | 1 |
| 13 | The application of RNAi to the medical genetics 显示文摘 | Wu D Wu BY Liang HY | 2005 | Beijing Da Xue Xue Bao2005，37，1： | 1 |
| 14 | Estate of Pack显示文摘 | Angela Liang The Argument Against a Physician's Duty to Warn for Genetic Diseases:the Conflicts Greated by Safer v | 1998 | Journal of Health Care Law and Policy1998，1，2： | 1 |
| 15 | Spectral correlative chromatogra- phy and its application to analysis of chromatographic fingerprints of herbal medicines显示文摘 | Li BY Hu Y Liang YZ | 2004 | J Sep Sci2004，27，78： | 1 |
| 16 | Primary results of salvage liver transplantation in the patients with unresectable recurrent hepatocellular carcinoma after initial liver resection显示文摘 | Liang BY Huang ZY Zhou SJ | 2014 | Hepatogastroenterology2014，61，135： | 1 |
| 17 | Multicomponent spectral correla- tive chromatography applied to complex herbal medicines 显示文摘 | Hu Y Liang YZ Li BY | 2004 | J Agric Food chem2004，52，26： | 1 |
| 18 | Long-term outcomes of repeat hepatic resection in patients with recurrent hepatocellular carcinoma and analysis of recurrent types and their prognosis: a single-center experience in China 显示文摘 | Huang ZY Liang BY Chen XP | 2012 | Ann Surg Oncol2012，19，： | 1 |
| 19 | Pharmacological effects of Niancian Milian Chuanbei Pipa Gao显示文摘 | Li ZL Dai BQ Liang AH Li GQ Yang Q Peng BY 1994 | 1994 | China Journal of Chinese Materia Medica1994，19，9： | 1 |
| 20 | AGT M235T polymorphisms and ischemic stroke risk:a meta-analysis显示文摘 | Liang BY Qin LL Wei HJ | 2013 | J Neurological Sci2013，331，： | 1 |
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