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| 1 | A structure-supporting,self-healing,and high permeating hydrogel bioink for establishment of diverse homogeneous tissue-like constructs显示文摘The ready-to-use,structure-supporting hydrogel bioink can shorten the time for ink preparation,ensure cell dispersion,and maintain the preset shape/microstructure without additional assistance during printing.Meanwhile,ink with high permeability might facilitate uniform cell growth in biological constructs,which is beneficial to homogeneous tissue repair.Unfortunately,current bioinks are hard to meet these requirements simultaneously in a simple way.Here,based on the fast dynamic crosslinking of aldehyde hyaluronic acid(AHA)/N-carboxymethyl chitosan(CMC)and the slow stable crosslinking of gelatin(GEL)/4-arm poly(ethylene glycol)succinimidyl glutarate(PEG-SG),we present a time-sharing structure-supporting(TSHSP)hydrogel bioink with high permeability,containing 1%AHA,0.75%CMC,1%GEL and 0.5%PEG-SG.The TSHSP hydrogel can facilitate printing with proper viscoelastic property and self-healing behavior.By crosslinking with 4%PEG-SG for only 3 min,the integrity of the cell-laden construct can last for 21 days due to the stable internal and external GEL/PEG-SG networks,and cells manifested long-term viability and spreading morphology.Nerve-like,muscle-like,and cartilage-like in vitro constructs exhibited homogeneous cell growth and remarkable biological specificities.This work provides not only a convenient and practical bioink for tissue engineering,targeted cell therapy,but also a new direction for hydrogel bioink development. | Hongqing Chen Fei Fei Xinda Li Zhenguo Nie Dezhi Zhou Libiao Liu Jing Zhang Haitao Zhang Zhou Fei Tao Xu | 2021 | Bioactive Materials2021，6，10： | 2 |
| 2 | Explore the Influence of Natural Environment to Highway Traffic Safety of Mountainous Area Based on DEA Theory 显示文摘 | Ming Zhang YuanHao Li HaiBo Shu LiBiao Liu | 2013 | The Engineering Index2013，，： | 1 |
| 3 | Detection of diverse viruses in alimentary specimens of bats in Macao显示文摘Bats carry a variety of viruses, and some of them cause public health problems. Macao, which is famous for its gambling industry, has a complex population structure. The globalization in such an international metropolis has enhanced the chance of disease transmission. Therefore, surveillance of zoonotic viruses is necessary for the early warning of potential emerging infectious diseases.Here, we report the first surveillance of bat viruses in Macao. In this study, we collected 1004 samples involving 10 bat species from 7 sites from April 2015 to May 2016, and examined the presence of viruses using nucleic acid-based methods. Coronaviruses, adenoviruses and paramyxoviruses were detected in these samples, with a high prevalence of coronaviruses. While,none was positive for hepatitis A virus, hepatitis E virus or hantavirus. Co-infections are not common in those bat species, but coronavirus HKU6 and adenovirus can be found commonly occurred in Myotis ricketti. | Jie Liang Xing-Lou Yang Bei Li Qi Liu Qin Zhang Hui Liu Hon-Pio Kan Kai-Chin Wong Si-Nga Chek Xiangyang He Xingwen Peng Zheng-Li Shi Yi Wu Libiao Zhang | 2017 | Virologica Sinica2017，32，3： | 1 |
| 4 | Pangolin HKU4-related coronaviruses found in greater bamboo bats from southern China显示文摘Coronavirus(CoV)spillover originating from game animals,particularly pangolins,is currently a significant concern.Meanwhile,vigilance is urgently needed for coronaviruses carried by bats,which are known as natural reservoirs of many coronaviruses.In this study,we collected 729 anal swabs of 20 different bat species from nine locations in Yunnan and Guangdong provinces,southern China,in 2016 and 2017,and described the molecular characteristics and genetic diversity of alphacoronaviruses(αCoVs)and betacoronaviruses(βCoVs)found in these bats.Using RT-PCR,we identified 58(8.0%)bat CoVs in nine bat species from six locations.Furthermore,using the Illumina platform,we obtained two representative full-length genomes of the bat CoVs,namely TyRo-CoV-162275 and TyRo-CoV-162269.Sequence analysis showed that TyRo-CoV-162275 shared the highest identity with Malayan pangolin(Manis javanica)HKU4-related coronaviruses(MjHKU4r-CoVs)from Guangxi Province,whereas TyRo-CoV-162269 was closely related to HKU33-CoV discovered in a greater bamboo bat(Tylonycteris robustula)from Guizhou Province.Notably,TyRo-CoV-162275 has a putative furin protease cleavage site in its S protein and is likely to utilize human dipeptidyl peptidase-4(hDPP4)as a cell-entry receptor,similar to MERSCoV.To the best of our knowledge,this is the first report of a bat HKU4r-CoV strain containing a furin protease cleavage site.These findings expand our understanding of coronavirus geographic and host distributions. | Min Guo Kai Zhao Xingwen Peng Xiangyang He Jin Deng Bo Wang Xinglou Yang Libiao Zhang | 2023 | Virologica Sinica2023，38，6： | 0 |
| 5 | A facile,versatile hydrogel bioink for 3D bioprinting benefits long-term subaqueous fidelity,cell viability and proliferation显示文摘Both of the long-term fidelity and cell viability of three-dimensional(3D)-bioprinted constructs are essential to precise soft tissue repair.However,the shrinking/swelling behavior of hydrogels brings about inadequate long-term fidelity of constructs,and bioinks containing excessive polymer are detrimental to cell viability.Here,we obtained a facile hydrogel by introducing 1%aldehyde hyaluronic acid(AHA)and 0.375%N-carboxymethyl chitosan(CMC),two polysaccharides with strong water absorption and water retention capacity,into classic gelatin(GEL,5%)–alginate(ALG,1%)ink.This GEL–ALG/CMC/AHA bioink possesses weak temperature dependence due to the Schiff base linkage of CMC/AHA and electrostatic interaction of CMC/ALG.We fabricated integrated constructs through traditional printing at room temperature and in vivo simulation printing at 37C.The printed cell-laden constructs can maintain subaqueous fidelity for 30 days after being reinforced by 3%calcium chloride for only 20 s.Flow cytometry results showed that the cell viability was 91.3861.55%on day 29,and the cells in the proliferation plateau at this time still maintained their dynamic renewal with a DNA replication rate of 6.0661.24%.This work provides a convenient and practical bioink option for 3D bioprinting in precise soft tissue repair. | Hongqing Chen Fei Fei Xinda Li Zhenguo Nie Dezhi Zhou Libiao Liu Jing Zhang Haitao Zhang Zhou Fei Tao Xu | 2021 | Regenerative Biomaterials2021，8，3： | 0 |
| 6 | Implementing Broadcast Encryption Scheme Using Bilinear Map and Group Characteristic显示文摘This paper introduced a novel method for implementing broadcast encryption. Our scheme takes advantages of bilinear map and group characteristic, and shifts most of the storage overhead to the public device instead of storing in the tamper-proof device which is a major problem on current implementation. Furthermore, the broadcast keys in our scheme could be reused periodically resulting in more operational efficiency. | ZHANG Yichun LIU Jianbo JIN Libiao LI Jianzeng | 2006 | Wuhan University Journal of Natural Sciences2006，11，6： | 0 |
| 7 | Comparative transcriptome analysis reveals molecular adaptations underlying distinct immunity and inverted resting posture in bats显示文摘Understanding how natural selection shapes unique traits in mammals is a central topic in evolutionary biology.The mammalian order Chiroptera(bats)is attractive for biologists as well as the general public due to their specific traits of extraordinary immunity and inverted resting posture.However,genomic resources for bats that occupy key phylogenetic positions are not sufficient,which hinders comprehensive investigation of the molecular mechanisms underpinning the origin of specific traits in bats.Here,we sequenced the transcriptomes of 5 bats that are phylo-genetically divergent and occupy key positions in the phylogenetic tree of bats.In combination with the available genomes of 19 bats and 21 other mammals,we built a database consisting of 10918 one-to-one ortholog genes and reconstructed phylogenetic relationships of these mammals.We found that genes related to immunity,bone remodeling,and cardiovascular system are targets of natural selection along the ancestral branch of bats.Further analyses revealed that the T cell receptor signaling pathway involved in immune adaptation is specifically enriched in bats.Moreover,molecular adaptations of bone remodeling,cardiovascular system,and balance sensing may help to explain the reverted resting posture in bats.Our study provides valuable transcriptome resources,enabling us to tentatively identify genetic changes associated with bat-specific traits.This work is among thefirst to advance our understanding of the molecular underpinnings of inverted resting posture in bats,which could provide insight into healthcare applications such as hypertension in humans. | Jinwei WU Libiao ZHANG Chao SHEN Simon Yung Wa SIN Caoqi LEI Huabin ZHAO | 2023 | Integrative Zoology2023，18，3： | 0 |
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