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| 1 | The Auxin-Regulated Protein ZmAuxRPI Coordinates the Balance between Root Growth and Stalk Rot Disease Resistance in Maize显示文摘To optimize fitness, plants must efficiently allocate their resources between growth and defense. Although phytohormone crosstalk has emerged as a major player in balancing growth and defense, the genetic basis by which plants man age this balance remai ns elusive. We previously ide ntified a quantitative disease . resistance locus, qRfg2, in maize (Zea mays) that protects against the fungal disease Gibberella stalk rot. Here, through map-based cloning, we demonstrate that the causal gene at qRfg2 is ZmAuxRPI, which encodes a plastid stroma-localized auxin-regulated protein. ZmAuxRPI responded quickly to pathogen challenge with a rapid yet transient reduction in expression that led to arrested root growth but enhanced resista nee to Gibberella stalk rot and Fusarium ear rot. ZmAuxRPI was show n to promote the biosynthesis of indole-3-acetic acid (IAA), while suppressing the formation of benzoxazinoid defense compounds. ZmAuxRPI presumably acts as a resource regulator modulating indole-3-glycerol phosphate and/or indole flux at the branch point between the IAA and benzoxazinoid biosynthetic pathways. The concerted interplay between IAA and benzoxazinoids can regulate the growth-defense balance in a timely and efficient manner to optimize plant fitness. | Jianrong Ye Tao Zhong Dongfeng Zhang Chuanyu Ma Lina Wang Lishan Yao Qianqian Zhang Mang Zhu Mingliang Xu | 2019 | Molecular Plant2019，12，3： | 19 |
| 2 | Combined genome-wide association study and transcriptome analysis reveal candidate genes for resistance to Fusarium ear rot in maize显示文摘Fusarium ear rot,caused by Fusarium verti-cillioides,is a devastating fungal disease in maize that re-duces yield and quality;moreover,F.verticillioides pro-duces fumonisin mycotoxins,which pose serious threats to human and animal health.Here,we performed a genome-wide association study(GWAS)under three en-vironmental conditions and identified 34 single-nucleotide polymorphisms(SNPs)that were significantly associated with Fusarium ear rot resistance.With reference to the maize B73 genome,69 genes that overlapped with or were adjacent to the significant SNPs were identified as potential resistance genes to Fusarium ear rot.Comparing transcriptomes of the most resistant and most susceptible lines during the very early response to Fusarium ear rot,we detected many differentially expressed genes enriched for pathways related to plant immune responses,such as plant hormone signal transduction,phenylpropanoid bio-synthesis,and cytochrome P450 metabolism.More than one-fourth of the potential resistance genes detected in the GWAS were differentially expressed in the tran-scriptome analysis,which allowed us to predict numbers of candidate genes for maize resistance to ear rot,in-cluding genes related to plant hormones,a MAP kinase,a PR5-like receptor kinase,and heat shock proteins.We propose that maize plants initiate early immune responses to Fusarium ear rot mainly by regulating the growth-defense balance and promoting biosynthesis of defense compounds. | Lishan Yao Yanmei Li Chuanyu Ma Lixiu Tong Feili Du Mingliang Xu | 2020 | Journal of Integrative Plant Biology2020，62，10： | 13 |
| 3 | Controlled synthesis of single cobalt atom catalysts via a facile one-pot pyrolysis for efficient oxygen reduction and hydrogen evolution reactions显示文摘Metal-nitrogen doped carbon catalysts(M-N/C) with abundantly accessible M-Nxsites, particularly single metal atom M-N/C(SAM-N/C), have been developed as a substitute for expensive Pt-based catalysts.These catalysts are used to increase the efficiency of otherwise sluggish oxygen reduction reactions(ORR) and hydrogen evolution reactions(HER). However, although the agglomerated metal nanoparticles are usually easy to form, they are very difficult to remove due to the protective surface-coating carbon layers, a factor that significantly hampers SAM-N/C fabrication. Herein, we report a one-step pyrolysis approach to successfully fabricate single cobalt atom Co-N/C(SACo-N/C) by using a Co2+-SCN-coordination compound as the metal precursor. Thanks to the decomposition of Co2+-SCN-compound at lower temperature than that of carbon layer deposition, Co-rich particles grow up to larger ones before carbon layers formation. Even though encapsulated by the carbon layers, it is difficult for the large Co-rich particle to be completely sealed. And thus, it makes the Co atoms possible to escape from incomplete carbon layer, to coordinate with nitrogen atoms, and to form SACo-N/C catalysts. This SACo-N/C exhibits excellent performances for both ORR(half-wave potential of 0.878 V) and HER(overpotential at 10 mA/cm2 of178 m V), and is thus a potential replacement for Pt-based catalysts. When SACo-N/C is integrated into a Zn-O2 battery, battery with high open-circuit voltage(1.536 V) has high peak power density(266 mW/cm2)and large gravimetric energy density(755 mA h/gZn) at current densities of 100 mA/cm2. Thus, we believe that this strategy may offer a new direction for the effective generation of SAM-N/C catalysts. | Yao Wang Linhui Chen Zhanxin Mao Lishan Peng Rui Xiang Xianyi Tang Jianghai Deng Zidong Wei Qiang Liao | 2019 | Science Bulletin2019，64，15： | 10 |
| 4 | SNX14 deficiency-induced defective axonal mitochondrial transport in Purkinje cells underlies cerebellar ataxia and can be reversed by valproate显示文摘Loss-of-function mutations in sorting nexin 14(SNX14)cause autosomal recessive spinocerebellar ataxia 20,which is a form of early-onset cerebellar ataxia that lacks molecular mechanisms and mouse models.We generated Snx14-deficient mouse models and observed severe motor deficits and cell-autonomous Purkinje cell degeneration.SNX14 deficiency disrupted microtubule organization and mitochondrial transport in axons by destabilizing the microtubule-severing enzyme spastin,which is implicated in dominant hereditary spastic paraplegia with cerebellar ataxia,and compromised axonal integrity and mitochondrial function.Axonal transport disruption and mitochondrial dysfunction further led to degeneration of high-energy-demanding Purkinje cells,which resulted in the pathogenesis of cerebellar ataxia.The antiepileptic drug valproate ameliorated motor deficits and cerebellar degeneration in Snx14-deficient mice via the restoration of mitochondrial transport and function in Purkinje cells.Our study revealed an unprecedented role for SNX14-dependent axonal transport in cerebellar ataxia,demonstrated the convergence of SNX14 and spastin in mitochondrial dysfunction,and suggested valproate as a potential therapeutic agent. | Hongfeng Zhang Yujuan Hong Weijie Yang Ruimin Wang Ting Yao Jian Wang Ke Liu Huilong Yuan Chaoqun Xu Yuanyuan Zhou Guanxian Li Lishan Zhang Hong Luo Xian Zhang Dan Du Hao Sun Qiuyang Zheng Yun-Wu Zhang Yingjun Zhao Ying Zhou Huaxi Xu Xin Wang | 2021 | National Science Review2021，8，7： | 1 |
| 5 | Sustainable silicon anodes facilitated via a double-layer interface engineering: Inner SiOx combined with outer nitrogen and boron co-doped carbon显示文摘Silicon-based(Si)materials are promising anodes for lithium-ion batteries(LIBs)because of their ultrahigh theoretical capacity of 4200 mA h g^(−1).However,commercial applications of Si anodes have been hindered by their drastic volume variation(∼300%)and low electrical conductivity.Here,to tackle the drawbacks,a hierarchical Si anode with double-layer coatings of a SiOx inner layer and a nitrogen(N),boron(B)co-doped carbon(C-NB)outer layer is elaborately designed by copyrolysis of Si-OH structures and a H3BO_(3)-doped polyaniline polymer on the Si surface.Compared with the pristine Si anodes(7mA h g^(−1) at 0.5 A g^(−1) after 340 cycles and 340 mA h g^(−1) at 5 A g^(−1)),the modified Si-based materials(Si@SiOx@C-NB nanospheres)present su perior cycling stability(reversible 1301 mA h g^(−1) at 0.5 A g^(−1) after 340 cycles)as well as excellent rate capability(690mA h g^(−1) at 5 A g^(−1))when used as anodes in LIBs.The unique double-layer coating structure,in which the inner amorphous SiOx layer acts as a buffer matrix and the outer defect-rich carbon enhances the electron diffusion of the whole anode,makes it possible to de liver excellent electrochemical properties.These results indicate that our double-layer coating strategy is a promising approach not only for the devel opment of sustainable Si anodes but also for the design of multielement-doped carbon nanomaterials. | Jun Zhou Yao Lu Lishan Yang Wenqiang Zhu Weifang Liu Yahui Yang Kaiyu Liu | 2022 | Carbon Energy2022，4，3： | 1 |
| 6 | Structure-guided manipulation of the regioselectivity of the cyclosporine A hydroxylase CYP-sb21 from Sebekia benihana显示文摘The cytochrome P450 enzyme CYP-sb21 from the rare actinomycete Sebekia benihana is capable of hydroxylating the immunosuppressive drug molecule cyclosporine A(CsA)primarily at the 4th N-methyl leucine(MeLeu4),giving rise toγ-hydroxy-N-methyl-L-Leu4-CsA(CsA-4-OH).This oxidative modification of CsA leads to dramatically reduced immunosuppressive activity while retaining the hair growth-promoting side-effect,thus demonstrating great application potential in both pharmaceutical and cosmetic industries.However,this P450 enzyme also hydroxylates CsA at the unwanted position of the 9th N-methyl leucine(MeLeu9),indicating that the regioselectivity needs to be improved for the development of CsA-4-OH into a commercial hair growth stimulator.Herein,we report the crystal structure of CYP-sb21 in its substrate-free form at 1.85Å.Together with sequence and 3D structure comparisons,Autodock-based substrate docking,molecular dynamics(MD)simulation,and site-directed mutagenesis,we identified a number of key residues including R294,E264,and M179 that can improve catalytic efficiency or change the regioselectivity of CYP-sb21 towards CsA,setting the stage for better enzymatic preparation of CsA-4-OH.This study also provides new insights into the substrate recognition and binding mechanism of P450 enzymes that accommodate bulky substrates. | Fengwei Li Li Ma Xingwang Zhang Jingfei Chen Feifei Qi Yinyue Huang Zepeng Qu Lishan Yao Wei Zhang Eung-Soo Kim Shengying Li | 2020 | Synthetic and Systems Biotechnology2020，5，3： | 1 |
| 7 | The advantages of penehyclidine hydrochloride over atropine in acute organophosphorus pesticide poisoning:A meta-analysis显示文摘Background:Penehyclidine hydrochloride(PHC)has been used for many years as an anticholinergic drug for the treatment of acute organophosphorus pesticide poisoning(AOPP).The purpose of this meta-analysis was to explore whether PHC has advantages over atropine in the use of anticholinergic drugs in AOPP.Methods:We searched Scopus,Embase,Cochrane,PubMed,ProQuest,Ovid,Web of Science,China Science and Technology Journal Database(VIP),Duxiu,Chinese Biomedical literature(CBM),WanFang,and Chinese National Knowledge Infrastructure(CNKI),from inception to March 2022.After all qualified randomized controlled trials(RCTs)were included,we conducted quality evaluation,data extraction,and statistical analysis.Statistics using risk ratios(RR),weighted mean difference(WMD),and standard mean difference(SMD).Results:Our meta-analysis included 20,797 subjects from 240 studies across 242 different hospitals in China.Compared with the atropine group,the PHC group showed decreased mortality rate(RR=0.20,95%confidence intervals[CI]:0.16–0.25,P<0.001),hospitalization time(WMD=-3.89,95%CI:-4.37 to-3.41,P<0.001),overall incidence rate of complications(RR=0.35,95%CI:0.28–0.43,P<0.001),overall incidence of adverse reactions(RR=0.19,95%CI:0.17–0.22,P<0.001),total symptom disappearance time(SMD=-2.13,95%CI:-2.35 to-1.90,P<0.001),time for cholinesterase activity to return to normal value 50–60%(SMD=-1.87,95%CI:-2.03 to-1.70,P<0.001),coma time(WMD=-5.57,95%CI:-7.20 to-3.95,P<0.001),and mechanical ventilation time(WMD=-2.16,95%CI:-2.79 to-1.53,P<0.001).Conclusion:PHC has several advantages over atropine as an anticholinergic drug in AOPP. | Siyao Zeng Lei Ma Lishan Yang Xiaodong Hu Cheng Wang Xinxin Guo Yi Li Yi Gou Yao Zhang Shengming Li Shaotong Zhang Xiaoxuan Wu Meihong Li Jing Lei Bingqian Li Chengfei Bi Like Ma Qingpeng Luo | 2023 | Journal of Intensive Medicine2023，3，2： | 0 |
| 8 | HCl guided morphology and conductivity of chiral PPy nanostructures toward efficient electromagnetic wave absorption显示文摘To solve the severe electromagnetic(EM)radiation from the widespread application of electronic equipment,we developed a simple template-guided oxypolymerization strategy to synthesize polypyrrole(PPy)planar helixes as an efficient EM wave(EMW)absorber,and systematically investigated the morphology-dependent chirality,conductivity,and microwave absorption properties.As HCl concentration[HCl]varied from 0 to 2.0 M,the morphology evolved from planar helix to 3D cross-linking network structures,the conductivity increased from 0.0019 to 0.0302 S/cm,and the EM parameters peaked at[HCl]=0.5 M.Compared to other absorbers,the PPy planar helix formed at[HCl]=0 M possessed wider absorption band(5.84 GHz),smaller matching thickness(1.6 mm),lower loading(25 wt.%),and intenser absorption(-48.17 dB).The reason lies in the strong attenuation capability,multiple resonances,multiple scattering,and good impedance matching generated by chiral PPy planar helixes with a distinctive helical configuration,doped heteroatoms(O,S),and a local conductive network.Our results suggest that PPy planar helixes offer great promise for fields such as chiral sensors,electronics,optics,chiral catalysis,and EMW absorption and shielding due to their distinctive morphology,tunable conductivity,and outstanding EMW absorption properties(EMWAPs). | YANG XiaoFen FAN BaoXin WANG XinXin WU LiShan YAO QiBin TONG GuoXiu WU WenHua CHEN DaBo | 2022 | Science China(Technological Sciences)2022，65，4： | 0 |
| 9 | PARALLEL GENETIC ALGORITHM ON PVM显示文摘PARALLELGENETICALGORITHMONPVM￥CuangmingLin;XinYao(DepartmentofComputerScience,ComputationalIntelligenceGroupUniversityCollege... | Cuangming Lin Xin Yao(Department of Computer Science, Computational Intelligence GroupUniversity College, The University of New South Wales, ADFA)lain Macleod(Computer Sciences Lab RSISE ANUCanberra, ACT, Australia 2600)Lishan Kang Yuping Chen(Institu | 1996 | Wuhan University Journal of Natural Sciences1996，1，Z1： | 0 |
| 10 | Size and near-surface engineering in weak-oxidative confined space to fabricate 4 nm L1_(0)-PtCo@Pt nanoparticles for oxygen reduction reaction显示文摘The near-surface structure of the Pt-based alloy including the surface and subsurface structures is prominent to their electrocatalytic performance.Modulating the near-surface structure of PtCo intermetallics with small particle size could efficiently optimize the binding force between Pt and oxygen and finally enhance its oxygen reduction reaction(ORR)performance.Here we simultaneously achieve the size controlling and surface modulation of intermetallic nanoparticles(NPs)in a weak-oxidative confined space with abundant uncoordinated oxygen atoms.1–2 atomic layers of concave Pt-rich surface were successfully constructed on 4 nm L1_(0)-PtCo core after removing Co–O species which is derived from the segregation of the subsurface Co to the surface induced by the uncoordinated oxygen atoms.Owing to the elaborate structure,PtCo-1000/C catalyst shows significant improvement in both activity(1.290 A∙mg_(Pt)^(−1)and 1.529 mA∙cm_(Pt)^(−2) at 0.9 V vs.reversible hydrogen electrode(RHE))and stability(85.2%of initial mass activity after accelerated degression tests(ADTs))even the production is scaled up to gram level.Density functional theory calculations suggest that the cave Pt site optimizes the protonation of*O,which finally boosts the ORR performance. | Yifei Liao Lishan Peng Chaoling Wu Yigang Yan Haijiao Xie Yungui Chen Yao Wang | 2023 | Nano Research2023，16，5： | 0 |
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