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| 1 | Genome-wide analysis of NDR1/HIN1-like genes in pepper(Capsicum annuum L.)and functional characterization of CaNHL4 under biotic and abiotic stresses显示文摘Plant NDR1/HIN1-like(NHL)genes play an important role in triggering plant defenses in response to biotic stresses.In this study,we performed a genome-wide identification of the NHL genes in pepper(Capsicum annuum L.)and characterized the functional roles of these CaNHL genes in response to abiotic stresses and infection by different pathogens.Phylogenetic analysis revealed that CaNHLs can be classified into five distinct subgroups,with each group containing generic and specific motifs.Regulatory element analysis showed that the majority of the promoter regions of the identified CaNHLs contain jasmonic acid(JA)-responsive and salicylic acid(SA)-responsive elements,and transcriptomic analysis revealed that CaNHL genes are expressed in all the examined tissues of pepper.The CaNHL1,CaNHL4,CaNHL6,CaNHL10,CaNHL11,and CaNHL12 genes were significantly upregulated under abiotic stress as well as in response to different pathogens,such as TMV,Phytophthora capsici and Pseudomonas syringae.In addition,we found that CaNHL4 localizes to the plasma membrane.CaNHL4-silenced pepper plants display significantly increased susceptibility to TMV,Phytophthora capsici and Pseudomonas syringae,exhibiting reduced expression of JA-related and SA-related genes and reduced ROS production.However,transient overexpression of CaNHL4 in pepper increases the expression of JArelated and SA-related genes,enhances the accumulation of ROS,and inhibits the infection of these three pathogens.Collectively,for the first time,we identified the NHL genes in pepper and demonstrated that CaNHL4 is involved in the production of ROS and that it also regulates the expression of JA-related and SA-related genes in response to different pathogens,suggesting that members of the CaNHL family play an essential role in the disease resistance of pepper. | Changyun Liu Haoran Peng Xinyu Li Chaolong Liu Xing Lv Xuefeng Wei Aihong Zou Jian Zhang Guangjin Fan Guanhua Ma Lisong Ma Xianchao Sun | 2020 | Horticulture Research2020，7，1： | 2 |
| 2 | Autoantibody against angiotensin AT1 receptor from preeclamptic patients enhances collagen-induced human platelet aggregation显示文摘Hypercoagulability,血小板激活,和 thrombocytopenia 是 preeclampsia 的主要特征,但是他们的负责的内在的分子的机制仍然保持阴暗。最近的研究证明了对血管收缩素 II 类型 1 受体(AT1-AA ) 的自身抗体为 preeclampsia 组成一个新奇风险因素。然而,在血小板激活的 AT1-AA 和在 preeclampsia 的 hypercoagulability 的角色从来没被调查过。在现在的学习,我们决定了 AT1-AA 是否在 vitro 支持血小板聚集,并且把潜在的位于 \O 下面机制。AT1-AA 被连接酶的 immunosorbent 试金检测。在从 preeclamptic 病人净化的免疫球蛋白 G 部分以后,积极 sera 被加到从健康志愿者,血小板聚集和细胞内部的 Ca2+ 孤立的血小板层次被检测。AT1-AA 显著地在 vitro 提高了导致骨胶原的血小板聚集,效果由 AT1 受体对手 losartan 堵住了。另外, AT1-AA 在整个实验增加了并且维持导致骨胶原的 cytosolic 钙集中。我们第一次证明 AT1-AA 显著地在 vitro 通过血管收缩素类型 1 受体激活支持导致骨胶原的血小板聚集,潜在地经由增加的细胞内部的 Ca2+ 集中,作为一个潜在的贡献者支持 AT1-AA 到 preeclampsia 的 hypercoagulable 状态。 | Kehua Bai Ke Wang Xiaoyu Li Jie Wang Jie Zhang LiSong Jin Wang Suli Zhang Wayne Bond Lau Xinliang Ma Huirong Liu | 2013 | Acta Biochimica et Biophysica Sinica2013，45，9： | 2 |
| 3 | Performance research of self regenerated absorption heat transformer cycle using TFE-NMP as working fluids显示文摘 | XU SHIMING LIU YANLI ZHANG LISONG | 2001 | International Journal of Refrigeration2001，24，6： | 1 |
| 4 | Perfor- mance research of self regenerated absorption heat transformer cycle using TFE-NMP as working fluids 显示文摘 | XU SHIMING LIU YANLI ZHANG LISONG | 2001 | International Journal of Refrigeration2001，24，6： | 1 |
| 5 | Tunable multiwavelength fiber laser based on a polarizationmaintaining erbiumdoped fiber and a polarizationcontroller显示文摘 | Zhang Chenfang Liu Lisong Liu Zhimin | 2011 | Optics Communications2011，284，1011： | 1 |
| 6 | Progress on deuterated potassium dihydrogen phosphate(DKDP)crystals for high power laser system application显示文摘In this review,we introduce the progress in the growth of large-aperture DKDP crystals and some aspects of crystal quality including determination of deuterium content,homogeneity of deuterium distribution,residual strains,nonlinear absorption,and laser-induced damage resistance for its application in high power laser system.Large-aperture high-quality DKDP crystal with deuteration level of 70%has been successfully grown by the traditional method,which can fabricate the large single-crystal optics with the size exceeding 400 mm.Neutron diffraction technique is an efficient method to research the deuterium content and 3D residual strains in single crystals.More efforts have been paid in the processes of purity of raw materials,continuous filtration technology,thermal annealing and laser conditioning for increasing the laser-induced damage threshold(LIDT)and these processes enable the currently grown crystals to meet the specifications of the laser system for inertial confinement fusion(ICF),although the laser damage mechanism and laser conditioning mechanism are still not well understood.The advancements on growth of large-aperture high-quality DKDP crystal would support the development of ICF in China. | Mingxia Xu Baoan Liu Lisong Zhang Hongkai Ren Qingtian Gu Xun Sun Shenglai Wang Xinguang Xu | 2022 | Light(Science & Applications)2022，11，9： | 1 |
| 7 | Microwave absorbing property of nano-Fe3O4/BaTiO3 composite system 显示文摘 | Zhou Kesheng Liu Gui Yin Lisong Ma Shihong Gao Songhua | 2005 | Journal of Central South University: Science and Technology2005，36，5： | 1 |
| 8 | Performance research of self regenerated absorption heat transformer cycle using TFE-NMP as working fluids显示文摘 | XU Shiming LIU Yanli ZHANG Lisong | 2001 | International Journal of Refrigeration2001，24，6： | 1 |
| 9 | Performance research of self regenerated absorption heat transformer cycle using TFE-NMP as working fluids显示文摘 | Xu Shiming Liu Yanli Zhang Lisong | 2001 | International Journal of Refrigeration2001，24，6： | 1 |
| 10 | Biomass, diversity and composition of epiphytic macrolichens in primary and secondary forests in the subtropical Ailao Mountains, SW China显示文摘 | Su Li Wenyao Liu Lisong Wang Wenzhang Ma Liang Song | 2011 | Forest Ecology and Management2011，，11： | 1 |
| 11 | Scratch behavior of high speed steels for hot rolls显示文摘The scratch behaviors of two high speed steels (HSS) for hot rolls were studied by a Micro-combi Tester, and the emphasis was placed on researching the relations between the microstructure and the scratch resistance property of different HSS. The experimental results indicate that during the scratch process, the carbides are embedded into the matrix, the penetration depth of different HSS is closely related with the matrix hardness, i.e., the higher the matrix hardness, the better the scratch resistance property; and in the matrix, the fine, dispersive carbides are beneficial to form steady friction between the indenter and the scratched materials, but the coarser carbides are easier to fall into pieces. | Li Zhou Dale Sun Changsheng Liu Chunguang Li Lisong Yao | 2008 | Journal of University of Science and Technology Beijing2008，15，4： | 1 |
| 12 | The evolution of carbides in the tempering process of semi-high speed steel cold rolls with nitrogen addition显示文摘This study analyzes the evolution of carbides in the tempering process of a newly developed semi-high speed steel cold forged roll with nitrogen addition using a transmission electron microscope (TEM) equipped with an energy dispersive X-ray (EDX).The precipitation sequence of the carbides was identified in the entire tempering process and it can be expressed as M3C→M3C+M(C,N)Ⅱ→M(C,N)Ⅱ+ M7C3Ⅱ+M23C6.The secondary hardening in the tempering process was mainly due to the dispersed precipitation of fine M(C,N)Ⅱ particles.The dissolution of M3C tempered at high temperatures induced the precipitation and coarsening of M7C3Ⅱ and M23C6. | WU Qiong SUN Dale YAO Lisong LIU Changsheng | 2009 | Baosteel Technical Research2009，3，2： | 0 |
| 13 | Phase-tailored assembly and encoding of dissipative soliton molecules显示文摘Self-assembly of particle-like dissipative solitons,in the presence of mutual interactions,emphasizes the vibrant concept of soliton molecules in varieties of laser resonators.Controllable manipulation of the molecular patterns,held by the degrees of freedom of internal motions,still remains challenging to explore more efficient and subtle tailoring approaches for the increasing demands.Here,we report a new phase-tailored quaternary encoding format based on the controllable internal assembly of dissipative soliton molecules.Artificial manipulation of the energy exchange of soliton-molecular elements stimulates the deterministic harnessing of the assemblies of internal dynamics.Self-assembled soliton molecules are tailored into four phase-defined regimes,thus constituting the phase-tailored quaternary encoding format.Such phase-tailored streams are endowed with great robustness and are resistant to significant timing jitter.All these results experimentally demonstrate the programmable phase tailoring and exemplify the application of the phase-tailored quaternary encoding,prospectively promoting high-capacity all-optical storage. | Yusong Liu Siyun Huang Zilong Li Haoguang Liu Yixiang Sun Ran Xia Lisong Yan Yiyang Luo Huanhuan Liu Gang Xu Qizhen Sun Xiahui Tang Perry Ping Shum | 2023 | Light(Science & Applications)2023，12，6： | 0 |
| 14 | A Novel High Volume Manufacturing Method for Defect-free and High-yield SiN Micro-sieve Membranes显示文摘Micro-sieves have been widely used in medical treatment,quarantine,environment,agriculture,pharmacy and food processing.However,the manufacturing and yield improvement have been difficult due to multiple challenges,such as the sieve unit release defect,cracking,and KOH corrosion.In this paper,we report process details and discuss technical difficulties which are usually the root-causes for process failures,and demonstrate a reliable and high yield production of SiNx micro-sieves processed with our novel method,which is also compatible with high volume manufacturing. | Yansong Liu Chao Zhao Lisong Dong Rui Chen Yayi Wei | 2018 | Journal of Microelectronic Manufacturing2018，1，1： | 0 |
| 15 | Comparative study of the effects of Tartary buckwheat seed and sprout consumption on the physiological indices and gut microbiota of C57BL/6J mice显示文摘Tartary buckwheat(Fagopyrum tataricum)is a well-known pseudocereal for its health and economic value.However,abundant antinutritional factors(ANFs)reduces its health benefits.As reported,germination can improve the nutritional profile of grains.In this study,we systematically evaluate the safety of Tartary buckwheat seeds(TB)and Tartary buckwheat sprouts(TBS)used as high active ingredients.After evaluating nutrition levels,bioactive compounds and ANFs in TBS during germinating,5^(th)-day TBS were selected as the raw material.C57BL/6J mice were gavaged daily with distilled water,TB,or TBS for 6 weeks.The physiological indices related to ANFs were determined.Results showed that the TB intake tends to generate negative effects on the gut microbiota,and organs.Additionally,upon TB intake,the Fe^(3+)content in serum,trypsin activity in pancreas and jejunum decreased,while the cytokine,IgE,and histamine levels in serum,water content in faeces,cytokine levels in liver and jejunum increased.Conversely,TBS did not induce any obvious negative effects on the above relevant indices and showed better lipid-lowering effect.Altogether,TBS are safer and more effective as a raw material to produce the functional food for long-term consumption with the intention of preventing and treating hyperlipidaemia. | Guohui Nan Haixia Zhao Qiong Wu Lisong Liu Zichao Guan Chenglei Li Huala Wu Dabing Xiang Qi Wu | 2024 | Food Science and Human Wellness2024，13，2： | 0 |
| 16 | Single Molecule Imaging in Living Cell with Optical Method显示文摘Significance, difficult, international developing actuality and our completed works for single molecules imaging in living cell with optical method are described respectively. Additionally we give out some suggestions for the technology development further. | Guiying Wang , Zhizhan XuZhihua Ding, Zhifeng Fan, Lisong Yang, Li Liu, Xiaoqing Deng , Qinghua WuShanghai Institute of Optics and Fine Mechanics, CAS, P.R.ChinaPO Box 800211, Shanghai, 201800, Tel :0086-021-69918800E-mail: gywsiofim@mail.shcnc.ac.cnYizhang Chen Medicine Institute of Zhejiang University | 2003 | 光学学报2003，23，S1： | 0 |
| 17 | High-order femtosecond vortices up to the 30th order generated from a powerful mode-locked Hermite-Gaussian laser显示文摘Femtosecond vortex beams are of great scientific and practical interest because of their unique phase properties in both the longitudinal and transverse modes,enabling multi-dimensional quantum control of light fields.Until now,generating femtosecond vortex beams for applications that simultaneously require ultrashort pulse duration,high power,high vortex order,and a low cost and compact laser source has been very challenging due to the limitations of available generation methods.Here,we present a compact apparatus that generates powerful high-order femtosecond vortex pulses via astigmatic mode conversion from a mode-locked Hermite-Gaussian Yb:KGW laser oscillator in a hybrid scheme using both the translation-based off-axis pumping and the angle-based non-collinear pumping techniques.This hybrid scheme enables the generation of femtosecond vortices with a continuously tunable vortex order from the 1st up to the 30th order,which is the highest order obtained from any femtosecond vortex laser source based on a mode-locked oscillator.The average powers and pulse durations of all resulting vortex pulses are several hundred milliwatts and<650 fs,respectively.In particular,424-fs 11th-order vortex pulses have been achieved with an average power of 1.6 W,several times more powerful than state-of-the-art oscillator-based femtosecond vortex sources. | Hongyu Liu Lisong Yan Hongshan Chen Xin Liu Heyan Liu Soo Hoon Chew Alexander Gliserin Qing Wang Jinwei Zhang | 2023 | Light(Science & Applications)2023，12，10： | 0 |
| 18 | Puccinia triticina effector protein Pt_21 interacts with wheat thaumatin-like protein TaTLP1 to inhibit its antifungal activity and suppress wheat apoplast immunity显示文摘Puccinia triticina(Pt), as the causal agent of wheat leaf rust, employs a plethora of effector proteins to modulate wheat immunity for successful colonization. Understanding the molecular mechanisms underlying Pt effector-mediated wheat susceptibility remains largely unexplored. In this study, an effector Pt_21 was identified to interact with the apoplast-localized wheat thaumatin-like protein TaTLP1 using a yeast two-hybrid assay and the Pt_21-TaTLP1 interaction was characterized. The interaction between Pt_21 and TaTLP1 was validated by in vivo co-immunoprecipitation assay. A TaTLP1 variant,TaTLP1C71A, that was identified by the site-directed mutagenesis failed to interact with Pt_21. Pt_21was able to suppress Bax-mediated cell death in leaves of Nicotiana benthamiana and inhibit TaTLP1-mediated antifungal activity. Furthermore, infiltration of recombinant protein Pt_21 into leaves of transgenic wheat line overexpressing TaTLP1 enhanced the disease development of leaf rust compared to that in wild-type leaves. These findings demonstrate that Pt_21 suppresses host defense response by directly targeting wheat TaTLP1 and inhibiting its antifungal activity, which broadens our understanding of the molecular mechanisms underlying Pt effector-mediated susceptibility in wheat. | Fei Wang Songsong Shen Zhongchi Cui Shitao Yuan Ping Qu Hui Jia Linshuo Meng Xiaoyu Hao Daqun Liu Lisong Ma Haiyan Wang | 2023 | The Crop Journal2023，11，5： | 0 |
| 19 | Dynamically observing the formation of MOFs-driven Co/N-doped carbon nanocomposites by in-situ transmission electron microscope and their application as high-efficient microwave absorbent显示文摘Metal-organic frameworks(MOFs)derived magnetic carbon-based nanocomposites have drawn widespread attentions due to the well distributed nanocrystals in carbon matrix.Dynamically observing the formation process is urgently needed.Herein,taking zeolitic imidazolate framework(ZIF)-67 as an example,the pyrolysis process is investigated by in-situ transmission electron microscopy(TEM)assisted with ex-situ characterizations.Co nanocrystals are evenly distributed in carbon at the initial stage of carbonization.By increasing pyrolysis temperature,the nanocrystals grow bigger and migrate to carbon surface.The carbon texture transfers from amorphous to crystalline at 600°C,and thoroughly converts at 800°C.In-situ heating TEM shows that more tiny Co nanocrystals move out from the carbon texture by increasing temperature from 700 to 800°C.At 1,000°C,some escaped tiny Co nanocrystals are volatilized and disappeared.The residual escaped Co nanocrystals catalyze the formation of carbon nanotubes(CNTs).Due to the synergistic effect between Co and carbon as well as porous structure,the nanocomposites show high-efficient microwave absorption performance,which can be tuned by pyrolysis temperature,heating rate,and mass fraction.When the mass fraction is 30 wt.%,the nanocomposites obtained at 600 or 700°C display remarkable microwave absorption with optimal reflection loss(RL)smaller than−70 dB and effective absorption band larger than 4.9 GHz.Combining the in-situ and ex-situ techniques,some key findings were observed:(1)graphitization of carbon;(2)volatilization of Co nanocrystals;(3)formation process of CNTs by Co catalyst.These findings are helpful to understand the formation of MOFs derived carbon-based composites and expand their practical applications,especially for microwave absorption. | You Zhou Xia Deng Hongna Xing Hongyang Zhao Yibo Liu Lisong Guo Juan Feng Wei Feng Yan Zong Xiuhong Zhu Xinghua Li Yong Peng Xinliang Zheng | 2022 | Nano Research2022，15，8： | 0 |
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