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| 1 | Introducing antisense waxy gene into rice seeds reduces grain amylose contents using a safe transgenic technique显示文摘An agrobacterium tumefaciens-mediated co-tr- ansformation method was established and successfully used to transfer an antisense waxy gene into rice. The binary vec- tor p13W4 contains an antisense waxy gene, a hygromycin resistance gene (hpt) and a report gene (gus). The other bi- nary vector p13W8 contains only an antisense waxy gene in the T-DNA region. Two separate strains of A. tumefaciens, containing p13W4 and p13W8 mixed at a ratio of 1︰9, were used to transform a high-yield rice strain Chao 2-10. A total of 34 transgenic plants were obtained. PCR analysis showed that 15 of them were co-transformed. After GUS staining, the T1seeds with GUS positive were eliminated. The GUS nega- tive T1 seeds were germinated, and the seedlings were ana- lyzed for the existence of antisense waxy gene. The transgenic T1 plants with only antisense waxy gene further were con- firmed through Southern blot analysis. The T2 seeds collected from marker-free T1 plants were used for amylose content analysis. The results showed that the amylose content in marker-free transgenic seeds reduced by up to 28.61% over the wild type rice. This marker-free transgenic rice with low amylose contents obtained in this project could be used to screen soft grain, and could also be used as a new rice re- source for eating quality improvement. | LIJianyue MAOWanxia YANGLijun ZHOUGenyu LIUJia YANQitao MIDong | 2005 | Chinese Science Bulletin2005，50，1： | 9 |
| 2 | Fusing moving average model and stationary wavelet decomposition for automatic incident detection:case study of Tokyo Expressway显示文摘Traffic congestion is a growing problem in urban areas all over the world.The transport sector has been in full swing event study on intelligent transportation system for automatic detection.The functionality of automatic incident detection on expressways is a primary objective of advanced traffic management system.In order to save lives and prevent secondary incidents,accurate and prompt incident detection is necessary.This paper presents a methodology that integrates moving average(MA)model with stationary wavelet decomposition for automatic incident detection,in which parameters of layer coefficient are extracted from the difference between the upstream and downstream occupancy.Unlike other wavelet-based method presented before,firsdy it smooths the raw data with MA model.Then it uses stationary wavelet to decompose,which can achieve accurate reconstruction of the signal,and does not shift the signal transfer coefficients.Thus,it can detect the incidents more accurately.The threshold to trigger incident alarm is also adjusted according to normal traffic condition with congestion.The methodology is validated with real data from Tokyo Expressway ultrasonic sensors.Experimental results show that it is accurate and effective,and that it can differentiate traffic accident from other condition such as recurring traffic congestion. | Qinghua Liu Edward Chung Liujia Zhai | 2014 | Journal of Traffic and Transportation Engineering(English Edition)2014，1，6： | 2 |
| 3 | Effects of Lateral Motion on the Creep Forces in Wheel/Rail Rolling Contact显示文摘EfectsofLateralMotionontheCrepForcesinWhel/RailRolingContact*JinXuesongZhangWeihuaHuLiujiaNationalTractionPowerLaboratory,Sou... | Jin Xuesong Zhang Weihua Hu Liujia National Traction Power Laboratory, Southwest Jiaotong University, Chengdu 610031, China | 1997 | Journal of Modern Transportation1997，14，1： | 2 |
| 4 | GeneticDiversityAnalysisofTibetanBrassicacampestrisI[J]显示文摘 | WANGJinxiong(王晋雄) LIUJia(刘佳) MAOLei(毛磊) etal | | 西藏农业科技0，，： | 1 |
| 5 | 显示文摘 | LIUJia GAOXun DUANHua-hua etal(刘佳 高勋 段花花 等) | 2012 | 激光杂志2012，33，1： | 1 |
| 6 | 显示文摘 | YangQizhi(杨奇志) LiuJia(刘佳) JiangXulin(蒋序林) | | 化学进展0，，： | 1 |
| 7 | Concentration and separation of vanadium from alkaline media by strong alkaline anion-exchange resin 717显示文摘With strong alkaline anion-exchange resin 717 as the sorbent and NaOH solution as the eluent,a study on the sorption from alkaline solution and elution of vanadium(V),silicon(Ⅳ),and aluminium(Ⅲ) was carried out.Different parameters affecting the sorption and elution process,including temperature,pH values as well as the ratio of resin to solution,were investigated.The results show that sorption degree of vana-dium(V) increases with a decrease of pH values,and V(V) ions are easier sorbed than Si(Ⅳ) and Al(Ⅲ) ions under the same conditions.The sorption degree of V(V),Si(Ⅳ),and Al(Ⅲ) at pH 9.14 for 15 min are 90.6%,33.5%,and 21.6%,respectively.Si(Ⅳ),Al(Ⅲ),and V(V) ions sorbed on 717 resin were eluted by use of 2 mol·L-1 NaOH solution;the desorption degree of V(V),Si(Ⅳ),and Al(Ⅲ) for 5 min are 81.7%,99.1%,and 99.3%,respectively. | HUANG Jinwen SU Peng WU Wenwei LIAO Sen QIN Huiquan WU Xuehang HE Xiaohu TAO Liujia FAN Yanjin | 2010 | Rare Metals2010，29，5： | 1 |
| 8 | Enhanced removal efficiency of bromate from aqueous solutions by nanoscale zero-valent iron immobilized on activated carbon显示文摘 | Chunhua Xu Xiaohong Wang Sheng Lin Liujia Zhu Yaming Chen | 2015 | Desalination and Water Treatment2015，，9： | 1 |
| 9 | 显示文摘 | YANLi(严丽) DINGYun-Jie(丁云杰) LIUJia(刘佳) ZHUHe-Jun(朱何俊) LINLi-Wn(林励吾) | | 催化学报0，，： | 1 |
| 10 | 显示文摘 | LIUJia(刘佳) YANGHao-Tian(杨浩田) ZHANGJing-Bo(张敬波) etal | | ActaJPhys-ChimSin(WuliHuaxueXuebao)0，，： | 1 |
| 11 | Photopatterning hybrid sol-gel glass materials prepared from ethylene tellurate and alkoxysilane 显示文摘 | LiuJia James M 0 Reilly Smith Thomas W | 2005 | Journal of Non- Crystalline Solids2005，351，： | 1 |
| 12 | ON THE GROWTH ORDER OF C_0-SEMIGROUPS, I显示文摘ONTHEGROWTHORDEROFC_0-SEMIGROUPS,I￥LUOYUEHU;ANDLIUJIAAbstract:Inthispaper,byusingthetheoryofsemigroupandspectrum,acomputation?.. | LUOYUEHU LIUJIA | 1995 | Applied Mathematics(A Journal of Chinese Universities)1995，10，1： | 0 |
| 13 | Design,Fabrication and Test of a VME Pressure Sensor显示文摘This paper presents the design,fabrication,packaging and measurement results of a vacuum microelectronic(VME)pressure sensor.A new configuration,named 'cathode-on-membrane',greatly reduces the difficulties in the packaging process and enables practical VME pressure sensors to be developed.Experimental studies and results demonstrate the characteristics of the sensor output current variation with applied pressure under different working voltages. | XIAShanhong CHENShaofeng LIUJia | 2003 | Chinese Journal of Electronics2003，12，1： | 0 |
| 14 | Supervised and Unsupervised Speaker Adaptation Using Confidence Measure显示文摘The speaker adaptation is an effective means of improving the performance of a speech recognition system,and it can be divided into supervised or unsupervised speaker adaptation.In this paper a kind of confidence measure based on the word lattice structure is proposed and is used for the supervised and unsupervised speaker adaptation.The reliability of the recognition results can be evaluated by the confidence measure,and the uncertain parts in the recognition results can be removed or be given smaller weights in the speaker adaptation process.The experiments show that the confidence can effectively eliminate the suspicious speech and improve the performance of the supervised and unsupervised adaptation considerably.The performance difference between the supervised and unsupervised adaptation is reduced by using the confidence measure based on the word lattice structure. | LIUJia LIHusheng LIUJing LIURunsheng | 2003 | Chinese Journal of Electronics2003，12，1： | 0 |
| 15 | Advances in China Space Medical Experiment Research显示文摘In 2021,China Space Station(CSS)was assembled and constructed in-orbit,which provided a broad space platform for space medicine and space life sciences research.Space medicine focuses on the systematic risks that restrict long-term manned spaceflight.It carries out systematic research on the impact and countermeasure technology of long-term weightlessness on astronauts’health,space radiation on astronauts’health,behavior and ability,advanced on-orbit monitoring and medical disposal technology,and traditional space medical application technology by the space medicine experiment platform on human body and cell.It has accumulated valuable data on space environmental effects and human factors research,established a“human system risk”research system,obtained new knowledge and discoveries of space medicine,and explored countermeasure technologies of new characteristics.Moreover,a series of achievements have been made in the basic research on the mechanism of the special space environmental effect,which provides a solid foundation for the implementation of space missions. | CHEN Hailong LIU Zhaoxia WANG Lidong DING Bai SHI Liujia WANG Chunyan MI Tao YU Hongqiang ZHAO Rui WANG Honghui CHEN Chao QU Lina MA Honglei JIANG Changhua LI Yongzhi WEI Ming LI Zhili XU Chong ZANG Peng CAO Ping LI Yinghui | 2022 | 空间科学学报2022，42，4： | 0 |
| 16 | Expression of Drosophila melanogaster acetylcholinesterase(DmAChE) gene splice variants in Pichia pastoris and evaluation of its sensitivity to organophosphorus pesticides显示文摘Acetylcholinesterase(AChE) is a key enzyme used to detect organophosphorus pesticide residues by the enzyme inhibition method.An accidental discovery of a mutant strain with AChE activity was made in our laboratory during the process of AChE expression by Pichia pastoris.The pPIC9 K-Drosophila melanogaster acetylcholinesterase(DmAChE)-like expression vector was constructed by codon optimization of this mutant strain,which was transformed into P.pastoris GS115,and positive clones were selected on yeast peptone dextrose(YPD) plate with G418 at 4.0 mg/mL.The GS115-pPIC9 K-DmAChE-like strain was subjected to 0.5% methanol induction expression for 120 h,with a protein band at 4.3 kDa found by the tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) pattern of the fermentation supernatant.After preliminary purification by ammonium sulfate precipitation,the enzyme activity was detected to be 76.9 U/(mL·min).In addition,the pesticide sensitivity test proved that DmAChE-like is selective and sensitive to organophosphorus pesticides. | Liujia SHI Fangfang YANG Yanyan XU Shoufeng WANG | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，3： | 0 |
| 17 | Adaptive bit allocation scheme in multi-cell cooperative block diagonalization systems with delayed and limited feedback显示文摘In multi-cell cooperative multi-input multi-output(MIMO) systems,base station(BS) can exchange and utilize channel state information(CSI) of adjacent cell users to manage co-channel interference.Users quantize the CSIs of desired channel and interference channels using finite-rate feedback links,then BS can generate cooperative block diagonalization(BD) precoding matrices using the obtained quantized CSI at transmitter to supress co-channel interference.In this paper,a novel adaptive bit allocation scheme is proposed to minimize the rate loss due to imperfect CSI.We derive the closed-form expression of rate loss caused by both channel delay and limited feedback.Based on the derived rate loss expression,the proposed scheme can adaptively allocate more bits to quantize the better channels with smaller delays and fewer bits to worse channels with larger delays.Simulation results show that the proposed scheme yields higher performance than other allocation schemes. | GUI Xin KANG Gui-xia Huang Dong-yan LIUjia ZHANG Ping | 2012 | The Journal of China Universities of Posts and Telecommunications2012，19，5： | 0 |
| 18 | High-performance flexible organic field effect transistors with print-based nanowires显示文摘Polymer nanowire(NW)organic field-effect transistors(OFETs)integrated on highly aligned large-area flexible substrates are candidate structures for the development of high-performance flexible electronics.This work presents a universal technique,coaxial focused electrohydrodynamic jet(CFEJ)printing technology,to fabricate highly aligned 90-nm-diameter polymer arrays.This method allows for the preparation of uniformly shaped and precisely positioned nanowires directly on flexible substrates without transfer,thus ensuring their electrical properties.Using indacenodithiophene-co-benzothiadiazole(IDT-BT)and poly(9,9-dioctylfluorene-co-benzothiadiazole)(F8-BT)as example materials,5 cm^(2) arrays were prepared with only minute size variations,which is extremely difficult to do using previously reported methods.According to 2D-GIXRD analysis,the molecules inside the nanowires mainly adopted face-onπ-stacking crystallite arrangements.This is quite different from the mixed arrangement of thin films.Nanowire-based OFETs exhibited a high average hole mobility of 1.1 cm^(2) V^(−1) s^(−1) and good device uniformity,indicating the applicability of CFEJ printing as a potential batch manufacturing and integration process for high-performance,scalable polymer nanowire-based OFET circuits.This technique can be used to fabricate various polymer arrays,enabling the use of organic polymer semiconductors in large-area,high-performance electronic devices and providing a new path for the fabrication of flexible displays and wearable electronics in the future. | Liangkun Lu Dazhi Wang Changchang Pu Yanyan Cao Yikang Li Pengfei Xu Xiangji Chen Chang Liu Shiwen Liang Liujia Suo Yan Cui Zhiyuan Zhao Yunlong Guo Junsheng Liang Yunqi Liu | 2023 | Microsystems & Nanoengineering2023，9，3： | 0 |
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