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| 1 | Salicylic acid and ethylene signaling pathways are involved in production of rice trypsin proteinase inhibitors induced by the leaf folder Cnaphalocrocis medinalis(Guenée)显示文摘The roles of signaling pathways in the production of trypsin proteinase inhibitors(TrypPIs)in rice infested by the leaf folder(LF) Cnaphalocrocis medinalis were studied.Infestation by LF increased TrypPI levels in the leaves of rice plants at the tillering,booting and flowering stages but decreased TrypPI levels at the ripening stage;TrypPI levels in rice stems did not increase at any developmental stage.Infestation by LF at the tillering stage systemically increased TrypPI levels in leaves but not in stems;it also enhanced salicylic acid(SA)levels in leaves and stems,and the ethylene level released from plants.However,LF infestation did not increase JA concentrations.Exogenous application of SA or ethylene enhanced TrypPI levels in the leaves and stems of plants at the tillering stage,whereas treatment with both SA and ethylene induced lower levels of TrypPIs than treatment with SA or ethylene alone,suggesting an antagonistic effect of SA and ethylene on TrypPIs induction.The results suggest that both SA and ethylene signaling pathways are involved in the production of TrypPIs in rice induced by LF;moreover,the antagonistic effect of SA and ethylene may explain the changes in TrypPI levels seen at different plant developmental stages and in different organs. | WANG Xia HU LiuCheng ZHOU GuoXin CHENG JiaAn LOU YongGen | 2011 | Chinese Science Bulletin2011，56，22： | 5 |
| 2 | Investigations on femtosecond laser-induced surface modification and periodic micropatterning with anti-friction properties on Ti6Al4V titanium alloy显示文摘Titanium alloys have a wide application in aerospace industries as it has greater strength and low density, but it has poor tribological properties. To improve its friction and wear performance, in present work, a femtosecond laser is used to directly irradiate the Ti6Al4V titanium alloy surface in air conditioning, which results in localized ablation and the formation of periodic microstructures but also a strong pressure wave, propagating the material inside. Through the optimization of processing parameters, surface modification and periodic micropatterning with effective anti-friction properties were successfully induced on the surface. After a treatment of femtosecond laser-induced surface modification(FsLSM), the surface microhardness was improved by 16.6% and compressive residual stress reached-746 MPa. Besides, laser-induced periodic surface structures(LIPSS) with a titanium oxide outer coating were fabricated uniformly on the titanium alloy surface. Rotary ball-on-disk wear experiments revealed that the average coefficient of friction(COF) and wear mass loss of the specimen with Fs LSM treatment were largely reduced by 68.9% and 90% as compared to that of untreated specimens, respectively. It was analyzed that the reason for the remarkable wear resistance was attributed to the comprehensive action of the generation of LIPSS, the titanium oxide outer coating, high amplitude compressive residual stress and gradient grain size distribution on the subsurface during the laser surface treatment. Since the findings here are broadly applicable to a wide spectrum of engineering metals and alloys, the present results offer unique pathways to enhancing the tribological performance of materials. | Xinlei PAN Weifeng HE Zhenbing CAI Xuede WANG Ping LIU Sihai LUO Liucheng ZHOU | 2022 | Chinese Journal of Aeronautics2022，35，4： | 2 |
| 3 | GeometricRectification of Satellite Imagery with Minimal GroundControl Using Space Oblique Mercator Projection Theory显示文摘 | Ren Liucheng Clarke Keith C Zhou Chenghu | 2010 | Cartography and Geographic Information Science2010，37，4： | 1 |
| 4 | Deforming TC6 titanium alloys at ultrahigh strain rates during multiple laser shock peening显示文摘 | Liucheng Zhou Yinghong Li Weifeng He Guangyu He Xiangfan Nie Donglin Chen Zhilin Lai Zhibin An | 2013 | Materials Science & Engineering A2013，，： | 1 |
| 5 | Deformation TC6 titanium alloys at ultrahigh strain rates during multiple laser shock pee- ning显示文摘 | Zhou Liucheng Li Yinghong He Weifeng | 2013 | Materials Science &- Engineering A2013，578，： | 1 |
| 6 | Targeted editing of intronic-splicing silencer enhancement of SMN2 Exon 7 inclusion by CRISPR/Case 9显示文摘Spinal muscular atrophy(SMA)is an autosomal recessive hereditary neuromuscular disease.Exon 7 and 8 of survival of motor neuron 1(SMN1)gene or only exon 7 homology deletion leads to the failure to produce a full-length SMN gene.The copy number of SMN2 gene with high homology of SMN1 affects the degree of disease and was the target gene for targeting therapy,in which splicing silencer in intron 7 was the key to suppress the inclusion of exon 7.In this study,we projected to use CRISPR/Case 9 for the targeted editing of intronic-splicing silencer(ISS)sequence to promote the inclusion of SMN2 exon 7 and increase the production of SMN2 full-length(FL)gene expression.It happens that there was a protospacer adjacent motif(PAM)at one end of the ISS sequence according to the design of sgRNA.The recombinant vector of sgRNA HSMN2 CRISPR/Case 9 was constructed and transfected into HEK293 cells.Sequencing results showed that the ISS sequence could be edited accurately and targeting in the predicted direction,in which deleting small fragments,inserting small amounts and mutation.Quantitative analysis of RT-PCR products by restriction enzyme of DdeI digestion showed that the FL of SMN2 increased by 8%(P<0.05).In the primary cultured chondrocytes of SMA mice,in which sgRNA HSMN2 CRISPR/Case9 recombinant vector transfection could increase the SMN2 FL gene by 23%(P<0.05)and significantly improve SMN protein levels(P<0.05).CRISPR/Case 9 is an effective tool for gene editing and therapy of hereditary diseases,but it is rarely reported in the treatment of SMA diseases.This study shows that CRISPR/Case 9 was first used for the precision target of ISS sequence editing,which can effectively promote the production of SMN2 FL gene expressions,in which there was an important clinical reference value. | LIUCHENG WU YI WANG LILI DU GUIQING JI RUI ZHOU ZEYI ZHAO JUN CHEN SHUNXING ZHU | 2021 | BIOCELL2021，45，6： | 0 |
| 7 | Anisotropic, biomorphic cellular Si_(3)N_(4) ceramics with directional well-aligned nanowhisker arrays based on wood-mimetic architectures显示文摘Inspired by the transport behavior of water and ions through the aligned channels in trees,we demonstrate a facile,scalable approach for constructing biomorphic cellular Si_(3)N_(4) ceramic frameworks with well-aligned nanowhisker arrays on the surface of directionally aligned microchannel alignments.Through a facile Y(NO_(3))_(3) solution infiltration into wood-derived carbon preforms and subsequent heat treatment,we can faultlessly duplicate the anisotropic wood architectures into free-standing bulk porous Si_(3)N_(4) ceramics.Firstly,α-Si3N4 microchannels were synthesized on the surface of CB-templates via carbothermal reduction nitridation(CRN).And then,homogeneous distributed Y–Si–O–N liquid phase on the walls of microchannel facilitated the anisotropicβ-Si3N4 grain growth to form nanowhisker arrays.The dense aligned microchannels with low-tortuosity enable excellent load carrying capacity and thermal conduction through the entire materials.As a result,the porous Si_(3)N_(4) ceramics exhibited an outstanding thermal conductivity(TC,k_(R)≈6.26 W·m^(−1)·K^(−1)),a superior flexural strength(σ_(L)≈29.4 MPa),and a relative high anisotropic ratio of TC(k_(R)/k_(L)=4.1).The orientation dependence of the microstructure–property relations may offer a promising perspective for the fabrication of multifunctional ceramics. | Songsong XU Xiaonan ZHOU Qiang ZHI Junjie GAO Liucheng HAO Zhongqi SHI Bo WANG Jianfeng YANG Kozo ISHIZAKI | 2022 | Journal of Advanced Ceramics2022，11，4： | 0 |
| 8 | Dihydroartemisinin ameliorates palmitate-induced apoptosis in cardiomyocytes via regulation on miR-133b/Sirt1 axis显示文摘Excessive fat ectopically deposited in the non-adipose tissues is considered as one of the leading causes of myopathy.The aim of this study was to investigate the role of Dihydroartemisinin(DHA)in palmitate(PAL)-incubated H9c2 cells(lipotoxicity-induced cell injury model).Cell viability of PAL-treated cells was determined by MTT assay,and apoptotic regulators were examined by qRT-PCR and western blot analysis,in the absence or in the presence of DHA,respectively.Expression levels of miR-133b and Sirt1 were also evaluated by qRT-PCR and western blotting examination.PAL decreased the viability of H9c2 cells and enhanced the expression of apoptotic genes.DHA reversed the effect of PAL on cell viability and lowed the level of Caspase3 and Bax.It also lowered the expression of miR-133b,while enhanced the expression of Bcl-2.Sirt1 was revealed as target of miR-133b through transcriptional regulation and the process was affected by DHA.DHA partially protected against the PAL-induced lipotoxicity by influencing the expression of miR-133b that hindered the activity of Sirt1.DHA may be used as a potential treatment in clinical management for lipotoxicity induced heart complications. | LONGJU QI XIAOYING XU BIN LI BO CHANG SHENGCUN WANG CHUN LIU LIUCHENG WU XIAODI ZHOU QINGHUA WANG | 2022 | BIOCELL2022，46，4： | 0 |
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