2篇
您的检索式：作者名="Liudan Wei"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A signal-amplified whole-cell biosensor for sensitive detection of Hg^2+ based on Hg^2+-enhanced reporter module显示文摘A signal-amplified mercury sensing biosensor with desired sensitivity was developed through firstly using the GFP mutant with fluorescence increasing response towards Hg^2+ as the reporter module.The developed biosensor showed response for Hg^2+ in a relatively wide range of 1–10,000 nmol/L,and the detection limit was improved one or two orders of magnitude in comparison with most metal-sensing biosensors in similar constructs.In addition,the biosensor could distinguish Hg^2+ easily from multiple metal ions and displayed strong adaptability to extensive p H conditions (pH 4.0–10.0).More importantly,the developed biosensor was able to provide an initial assessment of Hg^2+ spiked in the environmental water with the recoveries between 85.70%and 112.50%.The signal-amplified strategy performed by the modified reporter module will be widely applicable to many other wholecell biosensors,meeting the practical requirements with sufficient sensing performance. | Dan Wang Yanan Zheng Liudan Wei Na Wei Xiaosu Fan Shan Huang Qi Xiao | 2020 | Journal of Environmental Sciences2020，32，10： | 1 |
| 2 | Rapid field testing of mercury pollution by designed fluorescent biosensor and its cells-alginate hydrogel-based paper assay显示文摘With increasing industrial activities,mercury has been largely discharged into environment and caused serious environmental problems.The growing level of mercury pollution has become a huge threat to human health due to its significant biotoxicity.Therefore,the simple and fast means for on-site monitoring discharged mercury pollution are highly necessary to protect human beings from its pernicious effects in time.Herein,a'turn off'fluorescent biosensor(mCherry L199 C)for sensing Hg2+was successfully designed based on direct modification of the chromophore environment of fluorescent protein mCherry.For rapid screening and characterization,the designed variant of mCherry(mCherry L199 C)was directly expressed on outer-membrane of Escherichia coli cells by cell surface display technique.The fluorescent biosensor was characterized to have favorable response to Hg2+at micromole level among other metal ions and over a broad pH range.Further,the cells of the fluorescent biosensor were encapsulated in alginate hydrogel to develop the cells-alginate hydrogel-based paper.The cells-alginate hydrogel-based paper could detect mercury pollution in 5 min with simple operation process and inexpensive equipment,and it could keep fluorescence and activity stable at 4℃ for 24 hr,which would be a high-throughput screening tool in preliminarily reporting the presence of mercury pollution in natural setting. | Yanan Zheng Liudan Wei Linwei Duan Fangfang Yang Guixiang Huang Tianyi Xiao Min Wei Yanling Liang Huiting Yang Zhipeng Li Dan Wang | 2021 | Journal of Environmental Sciences2021，33，8： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员