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| 1 | Seasonal variations in the plant diet of the Chinese Monal revealed by fecal DNA metabarcoding analysis显示文摘The Chinese Monal(Lophophorus thuysii)is an alpine-obligate galliform species of global conservation priority.It has been listed as a first class protected wildlife species in China,requiring conservation actions during the 14 th Five-Year Plan period.However,the diet composition of Chinese Monal and its seasonal variations have rarely been studied,constraining the effective conservation of the species.Here,we investigated the plant diet composition of the Chinese Monal and its seasonal variations using a DNA metabarcoding approach on fecal samples.We collected 190 fecal samples of the Chinese Monals from the central Qionglai Mountains located in China,and analyzed the plant diet of this species using a DNA metabarcoding approach.Taxonomic profiling of higher plants in the fecal samples was performed using the second internal transcribed spacer(ITS2)amplicon.Downstream analyses,including rarefaction curves,nonmetric multidimensional scaling(NMDS)and permutational multivariate analysis of variance(PERMANOVA),were used to explore the seasonal variations in diet composition.The Chinese Monal foraged a wide range of plant recipes composed of 35 families and 83 genera throughout the year,with Brassicaceae,Apiaceae,and Poaceae as the dominant families,and Cardamine as the dominant genus.The species consumed plants from 62 genera from 28 families during the breeding season(n=81)and 66 genera from 31 families during the non-breeding season(n=109).Further,the plant diet composition significantly varied between the breeding and non-breeding seasons,especially for the frequency of occurrence and relative read abundances at genus level.Our study analyzed the plant diet of the Chinese Monal at a high resolution for the first time,and the results revealed that the seasonal variations in its plant diet composition was adapted to plant phenology and foraging strategy.Fritillaria species,a previously confirmed important food resource for the Chinese Monal,were not detected in any fecal samples,potentially due to overharvesting of Fritillaria bulbs for Traditional Chinese Medicine.Therefore,we highly recommend further restriction of herb gathering in Chinese Monal habitats to facilitate the conservation of this endangered species.Altogether,our study enriches essential ecological information for the Chinese Monal and also provides insights into conservation management for this endangered species. | Bin Wang Yun Li Guiquan Zhang Jian Yang Cao Deng Haoyu Hu Long Zhang Xiaoqin Xu Caiquan Zhou | 2022 | Avian Research2022，13，2： | 2 |
| 2 | Synthesis, characterization and biological activities of schiff base complexes derived from methyl-substituted salicylaldehyde and D-glucosamine,ML2显示文摘 | Huang Yonghua Shen Haoyu Long Shu | 2002 | Synthesis & Reactivity in Inorganic & Metal-Organic Chemistry2002，32，9： | 1 |
| 3 | Cell-free synthesis system-assisted pathway bottleneck diagnosis and engineering in Bacillus subtilis显示文摘Metabolic engineering is a key technology for cell factories construction by rewiring cellular resources to achieve efficient production of target chemicals.However,the existence of bottlenecks in synthetic pathway can seriously affect production efficiency,which is also one of the core issues for metabolic engineers to solve.Therefore,developing an approach for diagnosing potential metabolic bottlenecks in a faster and simpler manner is of great significance to accelerate cell factories construction.The cell-free reaction system based on cell lysates can transfer metabolic reactions from in vivo to in vitro,providing a flexible access to directly change protein and metabolite variables,thus provides a potential solution for rapid identification of bottlenecks.Here,bottleneck diagnosis of the N-acetylneuraminic acid(NeuAc)biosynthesis pathway in industrially important chassis microorganism Bacillus subtilis was performed using cell-free synthesis system.Specifically,a highly efficient B.subtilis cell-free system for NeuAc de novo synthesis was firstly constructed,which had a 305-fold NeuAc synthesis rate than that in vivo and enabled fast pathway dynamics analysis.Next,through the addition of all potential key intermediates in combination with substrate glucose respectively,it was found that insufficient phosphoenolpyruvate supply was one of the NeuAc pathway bottlenecks.Rational in vivo metabolic engineering of NeuAc-producing B.subtilis was further performed to eliminate the bottleneck.By down-regulating the expression level of pyruvate kinase throughout the growth phase or only in the stationary phase using inhibitory Nterminal coding sequences(NCSs)and growth-dependent regulatory NCSs respectively,the maximal NeuAc titer increased 2.0-fold.Our study provides a rapid method for bottleneck diagnosis,which may help to accelerate the cycle of design,build,test and learn cycle for metabolic engineering. | Rongzhen Tian Minghu Wang Jintian Shi Xiaolong Qin Haoyu Guo Xuanjie Jia Jianghua Li Long Liu Guocheng Du Jian Chen Yanfeng Liu | 2020 | Synthetic and Systems Biotechnology2020，5，3： | 1 |
| 4 | Kinetic Study of Homogeneous Rhodium-Catalyzed Ethanol Carbonylation Using Bis(diphenylphosphino)methane Ligands显示文摘Homogeneous rhodium-catalyzed carbonylation of ethanol is a potential route for the preparation of propionic acid,although its commercialization has been hindered by a low reaction rate.In this work,various bis(diphenylphosphine)ligands were evaluated in the rhodium-catalyzed reaction system in an effort to improve the reaction rate.By comparing the space-time yield and selectivity of the catalytic system in a Hastelloy autoclave reactor,the effects of the choice of ligand,ligand:rhodium ratio,carbon monoxide pressure,temperature,rhodium concentration,ethyl iodide content,and lithium iodide content were investigated.In the presence of the ligand bis(diphenylphosphino)methane disulfide(dppmS_(2)),the activity of the catalyst was improved significantly.The results revealed that the optimal conditions included a dppmS_(2):Rh molar ratio of 0.5:1,a rhodium concentration of 1000μg/g,an ethyl iodide content of 10%,and a lithium iodide content of 10%.The most suitable reaction temperature and carbon monoxide pressure were 463 K and 3.0 MPa,respectively,which resulted in a maximum space-time yield of 4.30 mol/(h·L).A kinetic model was established in the temperature range of 442-463 K,and the model data were regressed and validated,revealing an activation energy of 28.01 kJ/mol.Residual analysis and statistical tests demonstrated the reliability of the kinetic experiments. | Long Haoyu Xu Lin Song Jianhua Wang Xiaoping Wang Weichong Liu Dianhua | 2022 | China Petroleum Processing & Petrochemical Technology2022，24，2： | 0 |
| 5 | Serum Procalcitonin Levels on Admission Predict Death in Severe and Critical COVID-19 Patients in Wuhan, China显示文摘Background:We evaluated whether the serum procalcitonin(PCT)level could predict death in severe and critical coronavirus disease 2019(COVID-19)patients.Methods:This study included 129 COVID-19 patients.PCT levels on admission,treatment,and death were collected.The outcomes were compared.Results:The optimum cutoff value of the PCT level determined by receiver operator characteristic curve analysis to predict all-cause death was 0.085 ng/mL,with sensitivity of 95.7%and specifi city of 72.6%.Overall,78 patients had a PCT level below 0.085 ng/mL and 51 patients had a PCT level of 0.085 ng/mL or greater.High-PCT-level patients had lower levels of lymphocytes(P=0.001)and albumin(P=0.002)and higher levels of creatinine(P=0.024),D-dimer(P=0.002),and white blood cells,neutrocytes(P<0.001),high-sensitivity C-reactive protein(P<0.001),interleukin-6(P<0.001),interleukin-8(P=0.001),interleukin-10(P=0.001),tumor necrosis factor(P<0.001),erythrocyte sedimentation rate(P=0.001),and ferritin(P=0.001).During the 30-day observation period,23 patients died.Mortality was significantly higher in high-PCT-level patients than in patients with low PCT levels(43.1%vs.1.3%;P<0.001).The risks of death(P<0.0001)and ventilator use(P<0.0001)were increased in patients with PCT levels of 0.085 ng/mL or greater.Conclusions:A PCT level of 0.085 ng/mL or greater on admission could effectively predict death and ventilator use in severe and critical COVID-19 patients. | Han Jin Shengwen Yang Pengkang He Haoyu Weng Shengcong Liu Fan Yang Long Zhang Fangfang Fan Yan Zhang Jing Zhou Jianping Li | 2020 | Cardiovascular Innovations and Applications2020，，3： | 0 |
| 6 | Continuous Process Optimization and Computational Fluid Dynamics Simulation of a Methyl Anthranilate Jet Reactor显示文摘Methyl anthranilate(MA)is an important material for the synthesis of sodium saccharin,but the yield and quality of MA are not stable due to the batch operation conditions.In this study,the optimum conditions obtained by an orthogonal test in a batch reactor were a volume ratio of methanol to amide solution of 5:4,volume ratio of sodium hypochlorite to amide solution of 7:4,Hoffmann degradation reaction duration of 10 min,Hoffmann degradation reaction temperature of 10℃,esterification reaction duration of 10 min,and esterification reaction temperature of 40℃.The working flow velocity and allowable working viscosity range of a Venturi ejector inlet were both determined by a computational fluid dynamics(CFD)simulation.Based on the above experimental data,the continuous operation of the process was tested in a three-stage continuous reactor,which improved the product yield and quality of the process. | Chen Zhuohang Xu Lin Wang Xiaoping Song Jianhua Long Haoyu Li Xinping Liu Dianhua Xu Yongjing Chen Hebing Liu Hui Zhang Ying Lei Wei | 2023 | China Petroleum Processing & Petrochemical Technology2023，25，2： | 0 |
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