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| 1 | The herbal compound geniposide rescues formaldehyde-induced apoptosis in N2a neuroblastoma cells显示文摘The herbal medicine Tong Luo Jiu Nao(TLJN)contains geniposide(GP)and ginsenoside Rg1 at a molar ratio of 10:1.Rg1 is the major component of another herbal medicine,panax notoginseng saponin(PNS).TLJN has been shown to strengthen brain function in humans,and in animals it improves learning and memory.We have previously shown that TLJN reduces amyloidogenic processing in Alzheimer’s disease(AD)mouse models.Together this suggests TLJN may be a potential treatment for patients with dementia.Because chronic damage of the central nervous system by formaldehyde(FA)has been presented as a risk factor for age-associated cognitive dysfunction,in the present study we investigated the protective effect of both TLJN and GP in neuron-like cells exposed to FA.FA-exposed murine N2a neuroblastoma cells were incubated with TLJN,its main ingredient GP,as well as PNS,to measure cell viability and morphology,the rate of apoptosis and expression of genes encoding Akt,FOXO3,Bcl2 and p53.The CCK-8 assay,cytoskeletal staining and flow cytometry were used to test cell viability,morphology and apoptosis,respectively.Fluorescent quantitative real-time PCR(qRT-PCR)was used to monitor changes in gene expression,and HPLC to determine the rate of FA clearance.Treatment of N2a cells with 0.09 mmol L?1 FA for 24 h significantly reduced cell viability,changed cell morphology and promoted apoptosis.Both TLJN and GP conferred neuroprotection to FA-treated N2a cells,whereas PNS,which had to be used at lower concentrations because of its toxicity,did not.Our data demonstrate that TLJN can rescue neuronal damage caused by FA and that its main ingredient,GP,has a major role in this efficacy.This presents purified GP as a drug or lead compound for the treatment of AD. | CHEN JinYan SUN MengRu WANG XingHua LU Jing WEI Yan TAN Yan LIU Ying GTZ Jürgen HE RongQiao HUA Qian | 2014 | Science China(Life Sciences)2014，57，4： | 15 |
| 2 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 3 | Regional homogeneity approach to fMRI data analysis显示文摘 | Zang YF Jiang TZ Lu YL | | 0，，： | 1 |
| 4 | An ultrastructural study of rabbit re- nal tissues with experimental crush syndome显示文摘 | Shi JQ Lu JS Shi TZ | 1986 | J Med Colleges PLA1986，1，： | 1 |
| 5 | The sedative component of anesthesia is mediated by GABA (A) receptors in an endogenous sleep pathway 显示文摘 | Nelson LE Guo TZ Lu J | 2002 | Nat Neurosci2002，5，10： | 1 |
| 6 | Regional homogeneity approach to fMRI data analysis显示文摘 | Zang YF Jiang TZ Lu YL | 2004 | Neuroimage2004，22，： | 1 |
| 7 | Regional homogeneity approach to fMRI data analysis显示文摘 | Zang YF Jiang TZ Lu YL | | 0，，： | 1 |
| 8 | The a2-Adrenoceptor agonist dexmedetomidine converges on an endogenous sleep promoting pathway to ex- ert its sedative effects显示文摘 | Nelson LE Lu J Guo TZ | 2003 | Anesthesiology2003，98，2： | 1 |
| 9 | A Sodium leak current regulates pacemaker activity of adult central pattern generator neurons in Lymnaea stagnalis显示文摘 | Lu TZ Feng ZP | 2011 | PLo S One2011，6，4： | 1 |
| 10 | Regional homogeneity approach to fMRI data analysis显示文摘 | Zang YF Jiang TZ Lu YL | 2004 | Neuroimage2004，22，1： | 1 |
| 11 | The alpha2-adrenoceptor agonist dexrnedetomidine converges on an endogenous sleep-promoting pathway to exert its sedative effects显示文摘 | Nelson LE Lu J Guo TZ | 2003 | Anesthesiology2003，98，2： | 1 |
| 12 | The alpha2-adrenoceptor agonist dexmedetomidine converges on an endogenous sleep-promoting pathway to exert its sedative effects 显示文摘 | Nelson LE Lu J Guo TZ et d | 2003 | Anesthesiology2003，98，2： | 1 |
| 13 | Combined effects of TGFB1 +869 T/C and +915 G/C polymorphisms on acute rejection risk in solid organ transplant recipients:a systematic review and meta-analysis显示文摘 | Ge YZ Wu R Lu TZ | 2014 | PLoS One2014，9，93： | 1 |
| 14 | The α2-Adrenoceptor agonist dexmedetomidine converges on an endogenous sleep-promoting pathway to exert its sedative effects显示文摘 | Lu J Guo TZ | 2003 | Anesthesiology2003，98，： | 1 |
| 15 | The sedative component of anesthesia is mediated by GABA (A) receptors in an endogenous sleep pathway显示文摘 | Nelson LE Guo TZ Lu J | | 0，，10： | 1 |
| 16 | The a2-Adrenoceptor ago- nist dexmedtomidine converges on an endogenous sleep- promo- ring path- way to exert its sedative effects显示文摘 | Nelson LE Lu J Ggo TZ | 2003 | Anesthesiology2003，98，： | 1 |
| 17 | The sedative component of anesthesia is mediated by GABA(A) receptors in an endoge- nous sleep pathway 显示文摘 | Nelson LE Guo TZ Lu J | 2002 | Nature neuroscience2002，5，10： | 1 |
| 18 | The sedative component of anesthesia is mediated by GABA(A) receptors in an endogenous sleep pathway显示文摘 | Nelson LE Guo TZ Lu J | | 0，，： | 1 |
| 19 | The (x2 Adrenoceptor ago- nist dexm edetomidine converges on an endogenous sleep pro moting pathway to exertits sedative ef显示文摘 | Nelson LE Lu J Guo TZ | 2003 | Anesthesiolo gy2003，98，2： | 1 |
| 20 | The sedative compo- nent of anesthesia is mediated by GABA (A) receptors in an endogenous sleep pathway 显示文摘 | Nelson LE Guo TZ Lu J | 2002 | Nat Neurosei2002，5，： | 1 |
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