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1Performance Potential-based Neuro-dynamic Programming for SMDPs显示文摘An alpha-uniformized Markov chain is defined by the concept of equivalent infinitesimalgenerator for a semi-Markov decision process (SMDP) with both average- and discounted-criteria.According to the relations of their performance measures and performance potentials, the optimiza-tion of an SMDP can be realized by simulating the chain. For the critic model of neuro-dynamicprogramming (NDP), a neuro-policy iteration (NPI) algorithm is presented, and the performanceerror bound is shown as there are approximate error and improvement error in each iteration step.The obtained results may be extended to Markov systems, and have much applicability. Finally, anumerical example is provided.TANGHao YUANJi-Bin LUYang CHENGWen-Juan 2005自动化学报2005,31,4:10
2Endothelial CDS2 deficiency causes VEGFA-mediated vascular regression and tumor inhibition显示文摘The response of endothelial cells to signaling stimulation is critical for vascular morphogenesis,homeostasis and function.Vascular endothelial growth factor-a(VEGFA)has been commonly recognized as a pro-angiogenic factor in vertebrate developmental,physiological and pathological conditions for decades.Here we report a novel finding that genetic ablation of CDP-diacylglycerol synthetase-2(CDS2),a metabolic enzyme that controls phosphoinositide recycling,switches the output of VEGFA signaling from promoting angiogenesis to unexpectedly inducing vessel regression.Live imaging analysis uncovered the presence of reverse migration of the angiogenic endothelium in cds2 mutant zebrafish upon VEGFA stimulation,and endothelium regression also occurred in postnatal retina and implanted tumor models in mice.In tumor models,CDS2 deficiency enhanced the level of tumorsecreted VEGFA,which in-turn trapped tumors into a VEGFA-induced vessel regression situation,leading to suppression of tumor growth.Mechanistically,VEGFA stimulation reduced phosphatidylinositol(4,5)-bisphosphate(PIP2)availability in the absence of CDS2-controlled-phosphoinositide metabolism,subsequently causing phosphatidylinositol(3,4,5)-triphosphate(PIP3)deficiency and F0X01 activation to trigger regression of CDS2-null endothelium.Thus,our data indicate that the effect of VEGFA on vasculature is context-dependent and can be converted from angiogenesis to vascular regression.Wencao Zhao Le Cao Hanru Ying Wenjuan Zhang Dantong Li Xiaolong Ziiu Wenzhi Xue Shuang Wu Mengye Cao Cong Fu Haonan Qi Yimei Hao Yun-Chi Tang Jun Qin Tao PZhong Xiaoxi Lin Luyang Yu Xuri Li Lin Li Dianqing Wu Weijun Pan 2019Cell Research2019,29,11:9
3Effects of aging treatment on microstructure and mechanical properties of Cr25Ni35Nb and Cr35Ni45Nb furnace tube steel显示文摘Cr25Ni35Nb and Cr35Ni45Nb alloys are usually used in the ethylene cracking furnaces.However,premature failure of furnace tubes often occurs ahead of design life due to elevated temperature exposure conditions (1050-1100 ℃) and aggressive service environment.Effects of exposure temperature and time on microstructure and mechanical properties of Cr25Ni35Nb and Cr35Ni45Nb steel at aging temperature (1200 ℃) with various exposure time were simulated different service times at 1050 ℃.Change of mechanical properties at room temperature and elevated temperature (900 ℃) of the aged Cr25Ni35Nb and Cr35Ni45Nb steel were investigated.Under exposure at 1200 ℃,ultimate tensile and yield strength,elongation of Cr35Ni45Nb steel increase initially and then decrease,however,strength and ductility of Cr25Ni35Nb steel decrease with aging time increasing.Large amount of fine secondary carbide particles precipitated and dispersed in matrix of Cr35Ni45Nb steel,which increased strength and ductility for dispersion strengthening.However,the effect of the dispersion strengthening is weakened by needle-like secondary carbides.Strength and ductility decreased with fine secondary carbide particles growing.For Cr25Ni35Nb steel,few fine secondary carbide particles precipitated and dispersed in the matrix,and needle-like secondary carbides generated in the matrix,which causes strength and ductility decreased with aging time increasing.Limin SHEN Jianming GONG Yong JIANG Luyang GENG 2011Acta Metallurgica Sinica(English Letters)2011,24,3:8
4Generation of mouse anti-human urate anion exchanger antibody by genetic immunization and its identification显示文摘Background Human urate anion exchanger (hURAT1) as a major urate transporter expressed on renal tubular epithelial cells regulates blood urate level by reabsorbing uric acid. Antibody is an important tool to study hURAT1. This study aimed, by genetic immunization, to produce mouse anti-hURAT1 polyclonal antibody with high throughput and high specificity and to detect the location of hURAT1 in human kidney.Methods Human renal total RNA was isolated and the entire cDNA of hURAT1 was amplified by RT-PCR. The sequence of intracellular high antigenicity fragment (A280 to R349) was chosen by prediction software of protein antigenicity, and its cDNA was amplified from cDNA of hURAT1, and then cloned into pBQAP-TT vector to construct recombinant plasmid pBQAP-TT-hURAT1-210 for genetic immunization. Mice were inoculated with this recombinant plasmid and two other adjuvant plasmids, pCMVi-GMCSF and pCMVi-Flt3L, which helped to enhance the antibody’s generation. After four weeks, the mice were sacrificed to obtain the anti-hURAT1 antibody from serum. The antibody was identified by western blot analysis and immunohistochemistry. At the same time, rabbit anti-hURAT1 antibody was produced by protein immunization. The specificity and efficiency between the rabbit and mouse anti-hURAT1 antibody were compared by western blot analysis and immunohistochemistry. Results The entire cDNA of hURAT1 and cDNA of its intracellular high immunogenic fragment were amplified successfully. Recombinant plasmid pBQAP-TT-hURAT1-210 for genetic immunization was confirmed by restriction digestion and sequencing. Both!the mouse anti-hURAT1 antibody and rabbit anti-hURAT1 antibody recognized 58kD hURAT1 and 64kD glycosylated hURAT1 protein bands in western blot. Immunohistochemically, hURAT1 was located at the brush border membrane of renal proximal tubular cells. In addition, the throughput and specificity of the mouse anti-hURAT1 antibody were higher than those of the rabbit anti-hURAT1 antibody.Conclusion Genetic immunization can generate anti-hURAT1 polyclonal antibody of high throughput and specificity.XUGuo-shuang WUDi CHENXiang-mei SHISuo-zhu HONGQuan ZHANGPing LUYang 2005Chinese Medical Journal2005,,8:7
5The role of new energy in carbon neutral显示文摘Carbon dioxide is an important medium of the global carbon cycle,and has the dual properties of realizing the conversion of organic matter in the ecosystem and causing the greenhouse effect.The fixed or available carbon dioxide in the atmosphere is defined as'gray carbon',while the carbon dioxide that cannot be fixed or used and remains in the atmosphere is called'black carbon'.Carbon neutral is the consensus of human development,but its implementation still faces many challenges in politics,resources,technology,market,and energy structure,etc.It is proposed that carbon replacement,carbon emission reduction,carbon sequestration,and carbon cycle are the four main approaches to achieve carbon neutral,among which carbon replacement is the backbone.New energy has become the leading role of the third energy conversion and will dominate carbon neutral in the future.Nowadays,solar energy,wind energy,hydropower,nuclear energy and hydrogen energy are the main forces of new energy,helping the power sector to achieve low carbon emissions.'Green hydrogen'is the reserve force of new energy,helping further reduce carbon emissions in industrial and transportation fields.Artificial carbon conversion technology is a bridge connecting new energy and fossil energy,effectively reducing the carbon emissions of fossil energy.It is predicted that the peak value of China’s carbon dioxide emissions will reach 110×10^(8) t in 2030.The study predicts that China’s carbon emissions will drop to 22×10^(8) t,33×10^(8) t and 44×10^(8) t,respectively,in 2060 according to three scenarios of high,medium,and low levels.To realize carbon neutral in China,seven implementation suggestions have been put forward to build a new'three small and one large'energy structure in China and promote the realization of China’s energy independence strategy.ZOU Caineng XIONG Bo XUE Huaqing ZHENG Dewen GE Zhixin WANG Ying JIANG Luyang PAN Songqi WU Songtao 2021Petroleum Exploration and Development2021,48,2:6
6Comparison of labeling methods and time course of traumatic brain injury-induced cell death in mice显示文摘BACKGROUND:Various molecular mechanisms of cell death following traumatic brain injury have been previously described.However,the time course of cell death remains unclear.TUNEL and Fluoro-Jade B labeling have been widely used to label apoptotic cells and neuronal degeneration.Propidium iodide (PI) functions as a biomarker of cell death in vivo.OBJECTIVE:To explore the role of PI labeling compared to TUNEL and Fluoro-Jade B staining for detecting neural cell death,and to observe time course of traumatic brain injury-induced cell death in mice.DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment was performed at the Laboratory of Aging and Nervous Diseases,Soochow University from September 2007 to December 2008.MATERIALS:PI (B1221) was purchased from Sigma,USA.TUNEL kit was purchased from Roche Molecular Biochemicals,USA.Fluoro-Jade B was purchased from Chemicon,USA.METHODS:A total of 70 healthy,male,Kunming mice were randomly assigned to sham-surgery (n=5) and model (n=65) groups.Traumatic brain injury was established using the controlled cortical impact method.PI was intraperitoneally injected at 1 hour prior to animal sacrifice.MAIN OUTCOME MEASURES:TUNEL,Fluoro-Jade B,and PI-positive cells were quantified using a double-labeling method to determine the time course of traumatic brain injury-induced cell death.RESULTS:PI labeled cells in an earlier phase of cell death than TUNEL and Fluoro-Jade B labeling.PI-positive cells were observed immediately following injury,and the numbers rapidly increased in injured brain areas at 1 hour,peaked at 24-48 hours,and subsequently decreased at 3-21 days post-injury.TUNEL-labeled cells were significantly increased at 12 hours,while Fluoro-Jade B-labeled cells were increased at 6 hours after injury,with cells still visible at 6-48 hours post-injury.Moreover,a greater number of PI-positive cells were observed compared to TUNEL-and Fluoro-Jade B-labeled cells.CONCLUSION:PI labeling is more sensitive and reliable than TUNEL and Fluoro-Jade B staining for detecting cell death following traumatic brain injury.Moreover,PI labeling can function as a reliable marker to estimate the entire time course of cell death.Chengliang Luo Xiping Chen Hong Ni Qianqian Li Rui Yang Yuxia Sun Guangyou Zhu Luyang Tao 2010Neural Regeneration Research2010,5,9:6
7Imbalance of the reciprocally inhibitory loop between the ubiquitin-specific protease USP43 and EGFR/PI3K/AKT drives breast carcinogenesis显示文摘Lin He Xinhua Liu Jianguo Yang Wanjin Li Shumeng Liu Xujun Liu Ziran Yang Jie Ren Yue Wang Lin Shan Chengjian Guan Fei Pei Liandi Lei Yu Zhang Xia Yi Xiaohan Yang Jing Liang Rong Liu Luyang Sun Yongfeng Shang 2018Cell Research2018,28,9:5
8Truth Discovery with Memory Network显示文摘Truth discovery aims to resolve conflicts among multiple sources and find the truth. Conventional methods for truth discovery mainly investigate the mutual effect between the reliability of sources and the credibility of statements. These methods use real numbers, which have a lower representation capability than vectors to represent the reliability. In addition, neural networks have not been used for truth discovery. In this work, we propose memory-network-based models to address truth discovery. Our proposed models use feedforward and feedback memory networks to learn the representation of the credibility of statements. Specifically, our models adopt a memory mechanism to learn the reliability of sources for truth prediction. The proposed models use categorical and continuous data during model learning by automatically assigning different weights to the loss function on the basis of their own effects. Experimental results show that our proposed models outperform state-of-the-art methods for truth discovery.Luyang Li Bing Qin Wenjing Ren Ting Liu 2017Tsinghua Science and Technology2017,22,6:5
9Assessment of cognitive dysfunction in traumatic brain injury patients:a review显示文摘Traumatic brain injury(TBI)is one of the major causes of human mortality and morbidity in the world.Brain injury could affect the core of a person’s being–their thinking,memory,personality and behaviour.Electrophysiological markers from the human electroencephalogram and brain imaging provide a rich source of data which helps to elucidate specific processing impairments in TBI patients.To assess the cognitive and social function in traumatic brain injury patients,this review will focus on some of methods for assessing the disabling cognitive and social function deficits induced by TBI.There are many new technologies available to address TBI and recognition related questions.Integration of the various techniques will help to facilitate our comprehending of TBI,cognitive function and social function,and improve treatment and rehabilitation.Huiyan Sun Chengliang Luo Xiping Chen Luyang Tao 2017Forensic Sciences Research2017,2,4:5
10A correlation study of the expression of resistin and glycometabolism in muscle tissue after traumatic brain injury in rats显示文摘Jin Peng Zhu Lielie Zhang Jiasheng Xie Songling Pan Da Wen Hao Meng Weiyang Lin Luyang Chen Daqing 2014Chinese Journal of Traumatology2014,17,3:5
11Highly dispersed Co-Mo sulfide nanoparticles on reduced graphene oxide for lithium and sodium ion storage显示文摘A novel hybrid,highly dispersed spinel Co-Mo sulfide nanoparticles on reduced graphene oxide(Co3S4/CoMo2S4@rGO),is reported as anode for lithium and sodium ion storage.The hybrid is synthesized by one-step hydrothermal method but exhibits excellent lithium and sodium storage performances.The as-synthesized Co3S4/CoMo2S4@rGO presents reversible capacity of 595.4 mA·h·g^−1 and 408.8 mA·h·g^−1 after 100 cycles at a current density of 0.2 A·g^−1 for lithium and sodium ion storages,respectively.Such superior performances are attributed to the unique composition and structure of Co3S4/CoMo2S4@rGO.The rGO provides a good electronically conductive network and ensures the formation of spinel Co3S4/CoMo2S4 nanoparticles,the Co3S4/CoMo2S4 nanoparticles provide large reaction surface for lithium and sodium intercalation/deintercalation,and the spinel structure allows fast lithium and sodium ion diffusion in three dimensions.Yuqing Liao Chun Wu Yaotang Zhong Min Chen Luyang Cai Huirong Wang Xiang Liu Guozhong Cao Weishan Li 2020Nano Research2020,13,1:4
12Establishment of a new acute-on-chronic liver failure model显示文摘To establish an animal model of acute-on-chronic liver failure(ACLF) that would replicate the pathological process of ACLF in humans, rats were administered porcine serum(PS) for 11 weeks. Liver fibrosis was determined by pathological and biochemical assessments. The animals then were injected with D-galactosamine(D-gal) and lipopolysaccharide(LPS). The survival times of animals with cirrhosis and ACLF were determined over 48 h. Other animals were killed at 0, 4, 8 and 12 h after administration of D-gal/LPS. Liver injury was assessed by histopathological analysis and biochemical indices, and apoptosis was detected by Western blot and TUNEL analysis. After PS administration for 11 weeks the serum levels of hyaluronic acid and N-procollagen type III peptide increased significantly, and serious fibrosis and cirrhosis was observed at weeks 10 and 11. Cirrhotic rats were injected with D-gal/LPS to induced ACLF; the rate of mortality over 48 h was 80%. ALT and AST levels increased markedly at 4 h, but decreased significantly at8 and 12 h post-treatment. The total bilirubin, direct bilirubin, and total bile acids levels increased markedly at 8 and 12 h. Clotting times, TNF-α and IL-6 levels increased significantly, except for 12 h post-treatment.Apoptosis, inflammation and necrosis were elevated as determined by hematoxylin-eosin staining and TUNEL assays. BCL-2 levels decreased significantly, While BAX levels increased significantly.Cytochrome c expression peaked at 8 h postD-gal/LPS treatment. In conclusion, an ACLF model induced by PS and D-gal/LPS was established and the underlying mechanisms of ACLF development were explored.Fangfang Li Luyang Miao Hua Sun Yuyang Zhang Xiuqi Bao Dan Zhang 2017Acta Pharmaceutica Sinica B2017,7,3:4
13Phase control system for SSRF linac显示文摘The design of phase control system in Shanghai Synchrotron Radiation Facility (SSRF) linac is presented in this paper. And digital phase detecting algorithm, the key for phase control system, is fully described. The testing results for phase control system in 100MeV linac are discussed in detail.YIN Chongxian YU Luyang LIU Dekang 2008Nuclear Science and Techniques2008,19,3:4
14Design and measurement of signal processing system for cavity beam position monitor显示文摘In this paper,in order to achieve the output signal processing of cavity beam position monitor(CBPM),we develop a digital intermediate frequency receiver architecture based signal processing system,which consists of radio frequency(RF) front end and high speed data acquisition board.The beam position resolution in the CBPM signal processing system is superior to 1μm.Two signal processing algorithms,fast Fourier transform(FFT) and digital down converter(DDC),are evaluated offline using MATLAB platform,and both can be used to achieve,the CW input signal,position resolutions of 0.31μm and 0.10μm at -16 dBm.The DDC algorithm for its good compatibility is downloaded into the FPGA to realize online measurement,reaching the position resolution of 0.49μm due to truncation error.The whole system works well and the performance meets design target.WANG Baopeng LENG Yongbin YU Luyang ZHOU Weimin YUAN Renxian CHEN Zhichu 2013Nuclear Science and Techniques2013,24,2:3
15Surface disorder engineering in ZnCdS for cocatalyst free visible light driven hydrogen production显示文摘Metal chalcogenide solid solution,especially ZnCdS,has been intensively investigated in photocatalytic H_(2) generation due to their cost-effective synthetic procedure and adjustable band structures.In this work,we report on the defect engineering of ZnCdS with surface disorder layer by simple room temperature Li-ethylenediamine(Li-EDA)treatment.Experimental results confirm the formation of unusual Zn and S dual vacancies,where rich S vacancies(Vs)served as electron trapping sites,meanwhile Zn vacancies(Vzn)served as hole trapping sites.The refined structure significantly facilitates the photo charge carrier transfer and improves photocatalytic properties of ZnCdS.The disordered ZnCdS shows a highest photocatalytic H_(2) production rate of 33.6 mmol·g^(-1)·h^(-1) under visible light with superior photocatalytic stabilities,which is 7.3 times higher than pristine ZnCdS and 7 times of Pt(1 wt.%)loaded ZnCdS.Enna Ha Shuhong Ruan Danyang Li Yuanmin Zhu Yanping Chen Jiangyuan Qiu Zhaohui Chen Tingting Xu Jingyun Su Luyang Wang Junqing Hu 2022Nano Research2022,15,2:3
16The Effect of Super Volcanic Eruptions on Ozone Depletion in a Chemistry–Climate Model显示文摘With the gradual yet unequivocal phasing out of ozone depleting substances(ODSs), the environmental crisis caused by the discovery of an ozone hole over the Antarctic has lessened in severity and a promising recovery of the ozone layer is predicted in this century. However, strong volcanic activity can also cause ozone depletion that might be severe enough to threaten the existence of life on Earth. In this study, a transport model and a coupled chemistry–climate model were used to simulate the impacts of super volcanoes on ozone depletion. The volcanic eruptions in the experiments were the 1991 Mount Pinatubo eruption and a 100 × Pinatubo size eruption. The results show that the percentage of global mean total column ozone depletion in the 2050 RCP8.5 100 × Pinatubo scenario is approximately 6% compared to two years before the eruption and 6.4% in tropics. An identical simulation, 100 × Pinatubo eruption only with natural source ODSs, produces an ozone depletion of 2.5% compared to two years before the eruption, and with 4.4% loss in the tropics. Based on the model results,the reduced ODSs and stratospheric cooling lighten the ozone depletion after super volcanic eruption.Luyang XU Ke WEI Xue WU S. P. SMYSHLYAEV Wen CHEN V. Ya. GALIN 2019Advances in Atmospheric Sciences2019,36,8:3
173D printing of hydroxyapatite/tricalcium phosphate scaffold with hierarchical porous structure for bone regeneration显示文摘Three-dimensional(3D)-printed scaffolds have attracted considerable attention in recent years as they provide a suitable environment for bone cell tissue regeneration and can be customized in shape.Among many other challenges,the material composition and geometric structure have major impacts on the performance of scaffolds.Hydroxyapatite and tricalcium phosphate(HA/TCP),as the major constituents of natural bone and teeth,possess attractive biological properties and are widely used in bone scaffold fabrication.Many fabrication methods have been investigated in attempts to achieve HA/TCP scaffolds with microporous structure enabling cell growth and nutrient transport.However,current 3D printing methods can only achieve the fabrication of HA/TCP scaffolds with certain range of microporous structure.To overcome this challenge,we developed a slurry-based microscale mask image projection stereolithography,allowing us to form a HA/TCP-based photocurable suspension with complex geometry including biomimetic features and hierarchical porosity.Here,the curing performance and physical properties of the HA/TCP suspension were investigated,and a circular movement process for the fabrication of highly viscous HA/TCP suspension was developed.Based on these investigations,the scaffold composition was optimized.We determined that a 30 wt%HA/TCP scaffold with biomimetic hierarchical structure exhibited superior mechanical properties and porosity.Cell proliferation was investigated in vitro,and the surgery was conducted in a nude mouse in vivo model of long bone with cranial neural crest cells and bone marrow mesenchymal stem cells.The results showed our 3D-printed HA/TCP scaffold with biomimetic hierarchical structure is biocompatible and has sufficient mechanical strength for surgery.Xiangjia Li Yuan Yuan Luyang Liu Yuen‑Shan Leung Yiyu Chen Yuxing Guo Yang Chai Yong Chen 2020Bio-Design and Manufacturing2020,3,1:3
18Recent advances on the circadian gene PER2 and metabolic rhythm of lactation of mammary gland显示文摘Due to regulation by circadian rhythm, the lactation of the mammary gland has rhythmicity. As one of prominent members of period protein family which regulates biological rhythms, PER2 plays an important role in developing the milk duct and maintaining the polarity and the morphology of the mammary epithelium; at the same time, it is also closely related with the metabolism of milk protein and milk fat. This paper summarized recent researches on PER2 gene and related researches on mammary gland development and metabolism to provide some information for the studies of the theory and technology on physiological functions of the mammary gland and milk quality control.Mengzhi Wang Yujia Jing Liangyu Hu Jian Gao Luyang Ding Jun Zhang 2015Animal Nutrition2015,,4:2
19Constructions of vectorial Boolean functions with good cryptographic properties显示文摘Dear editor,Vectorial Boolean functions play an important role in designing certain stream cipher schemes,such as filtering generators and nonlinear combiners.To be used in the aforementioned schemes,vectorial Boolean functions must satisfy several criteria.The best affine and linear approximation attacks show that high nonlinearity is significant for designing cryptographically strong functions.Correlation-immune functions were first introduced by Siegenthaler[1]in order toLuyang LI Weiguo ZHANG 2016Science China(Information Sciences)2016,59,11:2
20Screening of Anti-tumor Effective Extracts from Sedum bulbiferum Makino and HPLC Determination of Quercetin and Kaempferol in This Herbal Medicine显示文摘[Objectives] To investigate the anti-tumor activity of Sedum bulbiferum Makino in vitro,and establish a HPLC method for determination of quercetin and kaempferol in S. bulbiferum. [Methods] The inhibitory activities of different extracts and total flavonoids of S. bulbiferum on proliferation of three kinds of cancer cells( Hep G2,EC109,SW480) were tested by MTT assay. HPLC method for determination of quercetin and kaempferol in S. bulbiferum was established. [Results]The growth and proliferation of the three kinds of cancer cells were all significantly inhibited by ethyl acetate fraction and total flavonoids isolated from S. bulbiferum. With each extraction concentration increasing,stronger anti-tumor activity was found. The linear ranges of quercetin and kaempferol were 0. 03-0. 36 μg( R = 0. 999 9) and0. 08-0. 96 μg( R = 0. 999 9),and the average recoveries were 98. 90%( RSD = 1. 15%) and 98. 27%( RSD = 1. 70%),respectively.[Conclusions]S. bulbiferum has significant anti-tumor activity in vitro. The HPLC method established was accurate,reproducible,and could be used for quality control of this crude drug.Liming MENG Xiangluan WAN Luyang LI Jing HU Pu CHEN Dingrong WAN 2019Medicinal Plant2019,10,3:2
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