|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Mechanism of antagonistic effects of Andrographis paniculata methanolic extract against Staphylococcus aureus显示文摘Objective: To investigate the effects of Andrographis paniculata(Burm.f.) Wall. Ex Nees(A. paniculata) on expressions and activities of catalase, superoxide dismutase and alkylhydroperoxide reductase C in Staphylococcus aureus(S. aureus) with respect to its survival in vitro. Methods: Antioxidative property of methanolic leaves extract of A. paniculata(0.06 mg/m L). Minimum inhibitory concentration(MIC) was determined by its ability to reduce hydrogen peroxide(H_2O_2) toxicity against S. aureus ATCC 25923(3.8×10~8 cfu/m L). Effects of the extract on expressions of kat A(encoding catalase), sod A and sod M [encoding superoxide dismutases(SODs)], and ahp C [encoding alkylhydroperoxide reductase C(AhpC)] in S. aureuswere determined by RT-q PCR and corresponding enzyme activity assays were performed. Nitroblue tetrazolium reduction(NBT) assay was performed to determine effects of the extract on intracellular and extracellular levels of O_2-in S. aureus. Results: Cells challenged with 7.5 mmol/L H_2O_2 showed 0% survival in 30 min whereas 25% survived after treatment with the extract and H_2O_2. Cells that were treated with the extract alone had 43% survival in the same exposure period. Expressions of sod A and sod M genes in extract-treated cells were lowered 0.8-fold and 0.7-fold, respectively with decrease in total SOD activity of 26.8 U compared to untreated cells, 32.4 U(P<0.05). In contrast, extract-treated S. aureus cells showed 3.3-fold increase in kat A expression with corresponding increase in catalase activity of 1.828 U compared to untreated cells which was 1.248 U,(P<0.05). More profoundly, ahp C expression was increased 61-fold in extract-treated cells,(P<0.05) with corresponding increase in Ahp C activity of 0.018 U compared to untreated cells, 0.012U,(P<0.05). Extract-treated cells had significantly lower intra-and extracellular O_2-levels with absorbance readings(A_(575nm)) of 0.340 and 0.524 compared to untreated cells which were 0.516 and 0.928(P<0.05), respectively. Conclusions: Taken together these results suggest that the low MIC of A. paniculatamethanolic leaves extract(0.06 mg/m L) reduce H_2O_2 toxicity and more importantly, was in itself effectively inhibitory against S. aureus. Further, our observations suggest that a probable mode of its inhibitory mechanism against S. aureus is by reducing total SOD activity through downregulation of sod A and sod M expressions. | Roslinah Mohamad Hussain Zayan Nabilah Rasyidah Abd.Razak Wan Mazlina Md Saad Maimunah Mustakim | 2017 | Asian Pacific Journal of Tropical Medicine2017，10，7： | 3 |
| 2 | Career plateaus:constructs ,consequences and coping strategies 显示文摘 | MAIMUNAH ISMAIL | 2008 | European Journal of Social Science2008，5，： | 1 |
| 3 | Meat species identification and Halal authentication analysis using mitochondrial DNA显示文摘 | Chandrika Murugaiah Zainon Mohd Noor Maimunah Mastakim Lesley Maurice Bilung Jinap Selamat Son Radu | 2009 | Meat Science2009，，1： | 1 |
| 4 | Human Capital Development and Its Impact on Firm Performance: Evidence from Developmental Economics 显示文摘 | Maran M Lawrence A Maimunah I | 2009 | The Journal of International Social Research2009，2，8： | 1 |
| 5 | Smart Farming for Sustainable Rice Production:An Insight into Application,Challenge,and Future Prospect显示文摘Rice has a huge impact on socio-economic growth,and ensuring its sustainability and optimal utilization is vital.This review provides an insight into the role of smart farming in enhancing rice productivity.The applications of smart farming in rice production including yield estimation,smart irrigation systems,monitoring disease and growth,and predicting rice quality and classifications are highlighted.The challenges of smart farming in sustainable rice production to enhance the understanding of researchers,policymakers,and stakeholders are discussed.Numerous efforts have been exerted to combat the issues in rice production in order to promote rice sector development.The effective implementation of smart farming in rice production has been facilitated by various technical advancements,particularly the integration of the Internet of Things and artificial intelligence.The future prospects of smart farming in transforming existing rice production practices are also elucidated.Through the utilization of smart farming,the rice industry can attain sustainable and resilient production systems that could mitigate environmental impact and safeguard food security.Thus,the rice industry holds a bright future in transforming current rice production practices into a new outlook in rice smart farming development. | Norhashila HASHIM Maimunah Mohd ALI Muhammad Razif MAHADI Ahmad Fikri ABDULLAH Aimrun WAYAYOK Muhamad Saufi Mohd KASSIM Askiah JAMALUDDIN | 2024 | Rice science2024，31，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员