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| 1 | MicroRNA let-7b targets important cell cycle molecules in malignant melanoma cells and interferes with anchorage-independent growth显示文摘一幅 microRNA 表示屏幕被执行从良性的 melanocytic nevi 在 laser-microdissected 纸巾分析 157 不同 microRNAs (n = 10 ) 并且主要恶意的黑瘤(n = 10 ) ,用量的即时 PCR。微分表示被作出对有利的裁决 72 microRNAs。microRNAs 的 let-7 家庭的成员是显著地, down 作为与良性的 nevi 相比在主要黑瘤调整了,为是的这些分子的一个可能的角色暗示肿瘤在恶意的黑瘤压制 ors。有趣地,类似的调查结果为肺和结肠癌被描述了。在黑瘤房间试管内 down 的 let-7b 的 Overexpression 调整了 cyclins 的表示 D1, D3,和 A,和 cyclin 依赖的 kinase (Cdk ) 4,所有哪个被描述了在黑瘤开发起一个作用。蛋白质表示上的 let-7b 的效果由于单个 mRNAs 的 3'-untranslated 区域(3'UTRs ) 指向,为 cyclin D1 由记者基因分析例示。与它细胞周期管理者上的 down modulating 效果一致, let-7b 禁止了细胞周期前进和黑瘤细胞的抛锚无关的生长。总起来说,这些调查结果不仅指向早黑瘤开发的新规章的机制,而且可以打开大街因为未来指向了这个肿瘤的治疗。 | Julia Schultz Peter Lorenz Gerd Gross Saleh Ibrahim Manfred Kunz | 2008 | Cell Research2008，18，5： | 45 |
| 2 | Long-term outcome and prognostic factors of patients with hilar cholangiocarcinoma显示文摘AIM: To evaluate the long-term outcome and prognostic factors of patients with hilar cholangiocarinoma. METHODS: Ninety-six consecutive patients underwent treatment for malignant hilar bile duct tumors during 1995–2005. Of the 96 patients, 20 were initially treated with surgery (n = 2 R0 / n = 18 R1). In non-operated patients, data analysis was performed retrospectively. RESULTS: Among the 96 patients, 76 were treated with endoscopic transpapillary (ERC, n = 45) and/or percutaneous transhepatic biliary drainage (PTBD, n = 31). The mean survival time of these 76 patients undergoing palliative endoscopic and/or percutaneous drainage was 359 ± 296 d. The mean survival time of patients with initial bilirubin levels > 10 mg/dL was significantly lower (P < 0.001) than patients with bilirubin levels < 10 mg/dL. The mean survival time of patients with Bismuth stage Ⅱ (n = 8), Ⅲ (n = 28) and Ⅳ (n = 40) was 496 ± 300 d, 441 ± 385 d and 274 ± 218 d, respectively. Thus, patients with advanced Bismuth stage showed a reduced mean survival time, but the difference was not significant. The type of biliary drainage had no significant beneficial effect on the mean survival time (ERC vs PTBD, P = 0.806). CONCLUSION: Initial bilirubin level is a significant prognostic factor for survival of patients. In contrast, age, tumor stage according to the Bismuth-Corlette classification, and types of intervention are not significant prognostic parameters for survival. Palliative treatment with endoscopic or percutaneous biliary drainage is still suboptimal, new diagnostic and therapeutic tools need to be evaluated. | Andreas Weber Sonja Landrock Jochen Schneider Manfred Stangl Bruno Neu Peter Born Meinhard Classen Thomas Rsch Roland M Schmid Christian Prinz | 2007 | World Journal of Gastroenterology2007，13，9： | 23 |
| 3 | Digital Earth 2020:towards the vision for the next decade显示文摘This position paper is the outcome of a brainstorming workshop organised by the International Society for Digital Earth(ISDE)in Beijing in March 2011.It argues that the vision of Digital Earth(DE)put forward by Vice-President Al Gore 13 years ago needs to be re-evaluated in the light of the many developments in the fields of information technology,data infrastructures and earth observation that have taken place since.The paper identifies the main policy,scientific and societal drivers for the development of DE and illustrates the multi-faceted nature of a new vision of DE grounding it with a few examples of potential applications.Because no single organisation can on its own develop all the aspects of DE,it is essential to develop a series of collaborations at the global level to turn the vision outlined in this paper into reality. | Max Craglia Kees de Bie Davina Jackson Martino Pesaresi Gabor Remetey-Fulopp Changlin Wang Alessandro Annoni Ling Bian Fred Campbell Manfred Ehlers John van Genderen Michael Goodchild Huadong Guo Anthony Lewis Richard Simpson Andrew Skidmore Peter Woodgate | 2012 | International Journal of Digital Earth2012，5，1： | 8 |
| 4 | Enhanced expression of interleukin-18 in serum and pancreas of patients with chronic pancreatitis显示文摘AIM: To investigate interleukin-18 (IL-18) in patients with chronic panreatitis (CP). METHODS: We studied 29 patients with CP and 30 healthy controls. Peripheral blood mononuclear cells (PBMC) were isolated and incubated with 50 mmol/L ethanol, lipopolysaccharide (LPS) (doses 25 g/L, 250 g/L, 2500 g/L) and both agents for 24 h. Levels of IL-18 in the supernatants, and levels of IL-18, IL-12, interferon (IFN)-γ and soluble CD14 in the serum were analysed by ELISA technique. Expression of IL-18 in PBMC was investigated by reverse-transcription (RT)-PCR. IL-18 protein levels in CP tissue and in normal pancreas were studied by ELISA technique. IL-18 levels in PBMC and pancreatic tissue were determined by Westernblot. Im-munohistochemistry for pancreatic IL-18 expression was performed. RESULTS: In patients, IL-18 serum levels were signifi-cantly enhanced by 76% (mean: 289.9 ± 167.7 ng/L) compared with controls (mean: 165.2 ± 43.6 ng/L; P < 0.0005). IL-12 levels were enhanced by 25% in patients (18.3 ± 7.3 ng/L) compared with controls (14.7 ± 6.8ng/L, P = 0.0576) although not reaching the statistical significance. IFN-γ and soluble CD14 levels were not in-creased. In vitro, LPS stimulated significantly and dose-dependently IL-18 secretion from PBMC. Incubation with ethanol reduced LPS-stimulated IL-18 secretion by about 50%. The mRNA expression of IL-18 in PBMC and the response of PBMC to ethanol and LPS was similar in CP patients and controls. In PBMC, no significant differences in IL-18 protein levels were detected between patients and controls. IL-18 protein levels were increased in CP tissues compared to normal pancreatic tissues. IL-18 was expressed by pancreatic acinar cells and by infiltrating inflammatory cells within the pancreas. CONCLUSION: IL-18 originates from the chronically inflammed pancreas and appears to be involved in the fibrotic destruction of the organ. | Alexander Schneider Stephan L Haas Ralf Hildenbrand Sren Siegmund Iris Reinhard Helmut Nakovics Manfred V Singer Peter Feick | 2006 | World Journal of Gastroenterology2006，12，40： | 4 |
| 5 | Role of glycosylation in TGF-β signaling and epithelial-to-mesenchymal transition in cancer显示文摘Glycosylation is a common posttranslational modification on membrane-associated and secreted proteins that is of pivotal importance for regulating cell functions.Aberrant glycosylation can lead to uncontrolled cell proliferation,cell-matrix interactions,migration and differentiation,and has been shown to be involved in cancer and other diseases.The epithelial-to-mesenchymal transition is a key step in the metastatic process by which cancer cells gain the ability to invade tissues and extravasate into the bloodstream.This cellular transformation process,which is associated by morphological change,loss of epithelial traits and gain of mesenchymal markers,is triggered by the secreted cytokine transforming growth factor-β(TGF-β).TGF-βbioactivity is carefully regulated,and its effects on cells are mediated by its receptors on the cell surface.In this review,we first provide a brief overview of major types of glycans,namely,N-glycans,O-glycans,glycosphingolipids and glycosaminoglycans that are involved in cancer progression.Thereafter,we summarize studies on how the glycosylation of TGF-βsignaling components regulates TGF-βsecretion,bioavailability and TGF-βreceptor function.Then,we review glycosylation changes associated with TGF-β-induced epithelial-to-mesenchymal transition in cancer.Identifying and understanding the mechanisms by which glycosylation affects TGF-βsignaling and downstream biological responses will facilitate the identification of glycans as biomarkers and enable novel therapeutic approaches. | Jing Zhang Peter ten Dijke Manfred Wuhrer Tao Zhang | 2021 | Protein & Cell2021，12，2： | 4 |
| 6 | Comprehensive lifestyle intervention vs soy protein-based meal regimen in non-alcoholic steatohepatitis显示文摘BACKGROUND Non-alcoholic steatohepatitis(NASH) has become one of the leading causes of liver disease in the western world. In obese patients weight reduction is recommended. Up to now there are no specific guidelines for weight loss in order to reduce hepatic fat content.AIM To investigate the effects of a 24-wk guided lifestyle intervention program compared to a meal replacement regimen based on soy protein.METHODS Twenty-six subjects with NASH participated in a randomized single-center study. They were randomly assigned to either meal replacement group(MR-G)with soy-yogurt-honey preparation or to guided lifestyle change group(LC-G)with endurance activity and nutrition counselling. Serum alanine transaminase(ALT), aspartate transaminase(AST), lipid parameters, and adipokines were measured. Liver fat content and lipid composition were determined by magnetic resonance imaging and magnetic resonance spectroscopy. Body fat mass and lean body mass were assessed using Bod Pod? device. Pre-and post-intervention monitoring of parameters was performed. Statistical analyses were conducted with SPSS software, results were expressed as median(interquartile range).RESULTS Twenty-two subjects(MR-G, n = 11 and LC-G, n = 11) completed the study(9 women, 13 men; age 52.1(15.0) years, body mass index(BMI) 32.3(3.3) kg/m^2).In both groups a significant weight loss was achieved(MR-G:-6.4(3.6) kg, P <0.01; LC-G:-9.1(10.4) kg, P < 0.01). BMI dropped in both groups(MR-G:-2.3(1.5)kg/m^2, P = 0.003; LC-G:-3.0(3.4) kg/m^2, P = 0.006). Internal fat and hepatic lipid content were markedly reduced in both groups in comparable amount. There was a strong correlation between reduction in liver fat and decrease in ALT.Likewise, both groups showed an improvement in glycemic control and lipid profile. Changes in adipokines, particularly in adiponectin and leptin were closely related to intrahepatic lipid changes.CONCLUSION Comprehensive lifestyle intervention and meal replacement regimen have comparable effects on body and liver fat, as well as decrease in markers of hepatic inflammation among NASH patients. | Peter Deibert Adhara Lazaro Denise Schaffner Aloys Berg Daniel Koenig Wolfgang Kreisel Manfred W Baumstark Daniel Steinmann Martin Buechert Thomas Lange | 2019 | World Journal of Gastroenterology2019，25，9： | 3 |
| 7 | Analysis of the nitric oxide-cyclic guanosine monophosphate pathway in experimental liver cirrhosis suggests phosphodiesterase-5 as potential target to treat portal hypertension显示文摘AIM To investigate the potential effect of inhibitors of phosphodiesterase-5(PDE-5) for therapy of portal hypertension in liver cirrhosis.METHODS In the rat model of thioacetamide-induced liver fibrosis/cirrhosis the nitric oxide-cyclic guanosine monophosphate(NO-cGMP) pathway was investigated. Expression and localization of PDE-5, the enzyme that converts vasodilating cGMP into inactive 5'-GMP, was in the focus of the study. Hepatic gene expression of key components of the NO-cGMP pathway was determined by qRT-PCR: Endothelial NO synthase(eNOS), inducible NO synthase(iNOS), soluble guanylate cyclase subunits α1 and β1(sGCa1, sGCb1), and PDE-5. Hepatic PDE-5 protein expression and localization were detected by immunohistochemistry. Serum cGMP concentrations were measured using ELISA. Acute effects of the PDE-5 inhibitor Sildenafil(0.1 mg/kg or 1.0 mg/kg) on portal and systemic hemodynamics were investigated using pressure transducers.RESULTS Hepatic gene expression of eNOS(2.2-fold; P = 0.003), sGCa1(1.7-fold; P = 0.003), sGCb1(3.0-fold; P = 0.003), and PDE-5(11-fold; P = 0.003) was increased in cirrhotic livers compared to healthy livers. Overexpression of PDE-5(7.7-fold; P = 0.006) was less pronounced in fibrotic livers. iNOS expression was only detected in fibrotic and cirrhotic livers. In healthy liver, PDE-5 protein was localized primarily in zone 3 hepatocytes and to a lesser extent in perisinusoidal cells. This zonation was disturbed in cirrhosis: PDE-5 protein expression in perisinusoidal cells was induced approximately 8-fold. In addition, PDE-5-expressing cells were also found in fibrous septa. Serum cGMP concentrations were reduced in rats with cirrhotic livers by approximately 40%. Inhibition of PDE-5 by Sildenafil caused a significant increase in serum cGMP concentrations [+ 64% in healthy rats(P = 0.024), + 85% in cirrhotic rats(P = 0.018)]. Concomitantly, the portal venous pressure was reduced by 19% in rats with liver cirrhosis. CONCLUSION Overexpression and abrogated zonation of PDE-5 likely contribute to the pathogenesis of cirrhotic portal hypertension. PDE-5 inhibition may therefore be a reasonable therapeutic approach for portal hypertension. | Denise Schaffner Adhara Lazaro Peter Deibert Peter Hasselblatt Patrick Stoll Lisa Fauth Manfred W Baumstark Irmgard Merfort Annette Schmitt-Graeff Wolfgang Kreisel | 2018 | World Journal of Gastroenterology2018，24，38： | 2 |
| 8 | Methylene blue-aided chromoendoscopy for the detection of intraepithelial neoplasia and colon cancer in ulcerative colitis显示文摘 | Ralf Kiesslich Johannes Fritsch Martin Holtmann Heinz H. Koehler Manfred Stolte Stephan Kanzler Bernhard Nafe Michael Jung Peter R. Galle Markus F. Neurath | 2003 | Gastroenterology2003，，4： | 2 |
| 9 | Chromoscopy-Guided Endomicroscopy Increases the Diagnostic Yield of Intraepithelial Neoplasia in Ulcerative Colitis显示文摘 | Ralf Kiesslich Martin Goetz Katharina Lammersdorf Constantin Schneider Juergen Burg Manfred Stolte Michael Vieth Bernhard Nafe Peter R. Galle Markus F. Neurath | 2007 | Gastroenterology2007，，3： | 2 |
| 10 | Electronic properties of wurtzite GaAs: A correlated structural, optical, and theoretical analysis of the same polytypic GaAs nanowire显示文摘III-V 复合半导体 nanowires 被 zincblende 和 wurtzite 结构的共存通常描绘。到目前为止,这 polytypism 阻碍了 GaAs 的亚稳的 wurtzite 阶段的电子性质的决心,它因此仍然保持高度争论。以获得新卓见进这个话题,我们跨相互关联的 nanoscale 光谱由有一样的 polytypic GaAs nanowire 的传播电子显微镜学分析的近地的扫描光显微镜学的成像分散了到 Si 晶片上。因此,空间地决定光致发光系列能明白地被分到其结构与解决格子的精确性被知道的 nanowire 片断。1.528 eV 的一个排放精力从扩大 zincblende 片断被观察,表明驱散的 nanowire 由于和它的支持的底层的相互作用大概在单轴的紧张下面。这些关键信息和为扩大纯 wurtzite 片断获得的排放精力被用来在一个 wurtzite 矩阵以及反的结构执行 zincblende 量磁盘的信封功能计算。在这些计算,我们改变了基本 bandgap,电子质量,和在 zincblende 和 wurtzite GaAs 之间的乐队偏移量。从有试验性的数据的这多参数比较,我们推断在 8 传导和一个原子价乐队之间的 bandgap 在没有紧张的 wurtzite GaAs 从 1.532 ~ 1.539 eV ,并且为 7-A bandgap 估计了 1.507 ~ 1.514 eV 的价值。 | Alexander Senichev Pierre Corfdir Oliver Brandt Manfred Ramsteiner Steffen Breuer Jorg Schilling Lutz Geelhaar Peter Werner | 2018 | Nano Research2018，11，9： | 2 |
| 11 | Correctness criteria for dynamic changes in workflow systems:A survey显示文摘 | Stefanie Rinderle Manfred Reichert Peter Dadam | 2004 | Data & Knowledge Engineering2004，50，1： | 1 |
| 12 | EB-PVDthermal barrier coatings for aeroengines and gas turbines显示文摘 | Manfred Peters Christoph Leyens Uwe Schulz | 2001 | Advanced Engineering Materials2001，3，4： | 1 |
| 13 | Investigations on mixed micelles and liposome preparations for parenteral use based on soya phosphatidylcholine显示文摘 | Manfred Durr Jorg Hager Josef Peter Lohr | 1994 | Eur J Pharm Biopharm1994，40，3： | 1 |
| 14 | DNA-based prediction of humanexternally visible characteristics in forensics:Motivations,scientific challenges,and ethical considerations显示文摘 | Manfred K Peter M S | 2009 | Forensic Sci Int:Genetics2009，3，： | 1 |
| 15 | Topological–histological investigation of the pterygium显示文摘 | Peter Seifert Jens Eckert Manfred Spitznas | 2001 | Graefe’s Archive for Clinical and Experimental Ophthalmology2001，，4： | 1 |
| 16 | Model predictive optimal averaging level control显示文摘 | Campo Peter J Manfred Morari | 1989 | AIChE J1989，35，4： | 1 |
| 17 | Some recent trends in research and technology of advanced thermal barrier coatings显示文摘 | Uwe Schulz Christoph Leyens Klaus Fritscher Manfred Peters Bilge Saruhan-Brings Odile Lavigne Jean-Marc Dorvaux Martine Poulain Remy Mévrel Micha?l Caliez | 2002 | Aerospace Science and Technology2002，，1： | 1 |
| 18 | Stable expression and functional characterization of a Na+-taurocholate cotransporting green fluorescent protein in human hepatoblastoma HepG2 cells显示文摘 | Gerd A. Kullak-Ublick Manfred G. Ismair Ralf Kubitz Marcus Schmitt Dieter H?ussinger Bruno Stieger Bruno Hagenbuch Peter J. Meier Ulrich Beuers Gustav Paumgartner | 2000 | Cytotechnology (-)2000，，1： | 1 |
| 19 | Mitigation of greenhouse gas emissions by anaerobic digestion of cattle slurry 显示文摘 | Joachim Clemens Manfred Trimborn Peter Weiland | 2006 | Agriculture Ecosystems and Environment2006，112，23： | 1 |
| 20 | Hormonal changes induced by partial rootzone drying of irrigated grapevine 显示文摘 | Manfred Stoll Brian Loveys Peter Dry | 2000 | Journal of Experimental Botany2000，51，350： | 1 |
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