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| 1 | Role and effect of vein-transplanted human umbilical cord mesenchymal stem cells in the repair of diabetic foot ulcers in rats显示文摘Diabetic foot ulcer(DFU)is one of diabetic complications,which is frequently present and tormented in diabetes mellitus.Most multipotent mesenchymal stromal cells(MSCs)are capable of immune evasion,providing an allogeneic,ready-to-use,cell product option for therapeutic applications.The beneficial effect of MSCs for the treatment of a variety of traumatic injuries,such as open wounds,has been extensively explored.In this study,a rat DFU model was used to simulate the pathophysiology of clinical patients and to investigate the localization of human umbilical cord mesenchymal stem cells(hUC-MSCs)after intravenous transplantation and its role in DFU healing,so as to evaluate the potential of hUC-MSCs in the treatment of DFU.The diabetic rat model was established by streptozotocin injection,which was used to create full-thickness foot dorsal skin wounds to mimic DFU by a 6-mm skin biopsy punch and a Westcott scissor.The hUC-MSCs were transplanted through femoral vein,and the ulcer cicatrization situation and the fate of hUC-MSCs were evaluated.Our data suggest that intravenously transplantated hUC-MSCs have the ability to migrate and locate to the wound tissue and are helpful to wound healing in DFU rats,partly by regulating inflammation,trans-differentiation and providing growth factors that promote angiogenesis,cell proliferation and collagen deposition.Herein,we demonstrate that hUC-MSC transplantation is able to accelerate DFU healing in rats and transplantation of exogenous stem cells may be a potential strategy for clinical application in DFUs. | Rongfeng Shi Weishuai Lian Yinpeng Jin Chuanwu Cao Shilong Han Xiaohu Yang Suming Zhao Maoquan Li Hui Zhao | 2020 | Acta Biochimica et Biophysica Sinica2020，52，6： | 13 |
| 2 | Expression of Rab25 correlates with the invasion and metastasis of gastric cancer显示文摘The objective of this study was to determine the expression of the important vesicle trafficking- regulating factor Rab25 in human gastric cancer tissues, to analyze the correlation between Rab25 protein expression with gastric cancer occurrence and development, and to discuss the correlation of Rab25 protein expression with gastric cancer cell metastasis. The overall aim was to provide experimental evidence that can be used to design future biological treatments of human gastric cancer. Human gastric cancer tissue and the adjacent normal gastric tissue were surgically removed, and immunohistochemistry and Western blotting were used to detect Rab25 protein expression. The correlation between Rab25 protein expression with the development and pathological characteristics of gastric cancer was analyzed. Using RNAi, Rab25 expression was reduced in the gastric cancer cell line MGC80-3, and the changes in MGC80-3 cell invasiveness were then monitored. Immunohistochemistry showed that the Rab25 protein expression rates were 78.21% and 23.08% in gastric carcinoma and the adjacent normal gastric tissue, respectively. Immunohistochemistry and Western blot results showed that Rab25 protein expression in gastric cancer was significantly higher than in adjacent normal gastric tissues (P<0.01). Less differentiated gastric cancer cells had higher expression of Rab25 protein (P<0.01). Gastric carcinomas from patients with a late pathological stage (III-IV) had significantly higher Rab25 protein expression than early stage (I-II) patients (P<0.01). Gastric carcinomas from patients with lymph node metastasis had significantly higher Rab25 protein expression than lymph node metastasis- free patients (P<0.01). Gastric carcinomas from patients with distant metastases had significantly higher Rab25 protein expression than the distant metastasis-negative patients (P<0.01). Rab25 protein expression in gastric cancer was not affected by the patients , sex, age, or tumor size (P>0.05). MGC80-3 cells transfected with Rab25 siRNA had significantly lower Rab25 protein expression (P<0.01) and a significantly lower number of cells that passed through a Transwell chamber compared with non-transfected controls and the transfected control group (P<0.01). Rab25 protein expression is associated with the development of gastric cancer. siRNA knockdown of Rab25 protein expression in MGC80-3 gastric cancer cells reduced MGC80-3 cell invasiveness and provided experimental evidence for potential future biological treatment strategies of human gastric cancer. | Chuanwu Cao Chenhui Lu Jichong Xu Jiaxing Zhang Jun Zhang Maoquan Li | 2013 | Chinese Journal of Cancer Research2013，25，2： | 12 |
| 3 | MiR-31 regulates the function of diabetic endothelial progenitor cells by targeting Satb2显示文摘Endothelial 与糖尿病在病人失灵被知道导致脉管的疾病,并且 endothelial 祖先房间(EPC ) 为脉管的内皮细胞层的功能的保藏是不可缺少的。MicroRNA-31 (miR-31 ) 被发现了能调制干细胞的区别。然而, miR-31 怎么在糖尿病的 EPC 工作,仍然是不清楚的。这研究的目的是调查 miR-31 怎么调整糖尿病的 EPC 功能。在当前的学习, miR-31 表示在正常、糖尿病的 EPC 之间被比较。Satb2 根据计算预言在 EPC 作为 miR-31 的一个机能上地相关的目标被认出。我们也以它的 anti-apoptotic 效果探索了 miR-31 的角色。在 miR-31 表示的显著举起在糖尿病的 EPC 被发现,并且这提高的表情在高葡萄糖下面导致了压制的房间增长。miR-31 指向 Satb2,这也被发现,导致 anti-apoptotic 效果和 EPC 的功能的维护。而且,展出的 Satb2 击倒在健康、糖尿病的题目的 EPC 的增长和移植上的禁止的效果,它显示出象 miR-31 overexpression 的一样的趋势。相反地, Satb2 的 overexpression 显示出相反的效果。而且, Satb2 的 overexpression 在健康、糖尿病的题目稀释了 miR-31-induced 迁居和形成殖民地的能力减小和 EPC 的 apoptosis 正式就职。在糖尿病的 EPC,提高了葡萄糖水平被发现到起来调整 miR-31 表示,它接着提高了 EPC 的 malfunction 和死亡。在结论,我们的结果显示 miR-31 的起来规定可以由指向 Satb2 在糖尿病位于 endothelial 机能障碍下面。 | Weishuai Lian Xiaoxiao Hu Rongfeng Shi Shilong Han Chuanwu Cao Kun Wang Maoquan Li | 2018 | Acta Biochimica et Biophysica Sinica2018，50，4： | 7 |
| 4 | Guidelines and standards for comprehensive clinical diagnosis and interventional treatment for diabetic foot in China(Issue 7.0)显示文摘Diabetic foot(DF)is one of the most common complications of diabetes and is associated with high morbidity,disability,lethality and low cure-rate.The clinical diagnosis and treatment of DF need to be standardized.The Chinese Diabetic Foot Cell and Interventional Therapy Technology Alliance has released six editions of guidelines and standards for clinical diagnosis and interventional treatment of DF,which filled the gap in the domestic DF treatment standard and played an important role in improving the level of diagnosis and treatment in China.In line with the latest developments in diagnosis and treatment,the Alliance,along with other 89 institutions,developed and issued the new edition based on the sixth edition to help standardize the clinical diagnosis and treatment of DF in China. | Maoquan Li | 2021 | Journal of Interventional Medicine2021，4，3： | 6 |
| 5 | MicroRNA-182 promotes pancreatic cancer cell proliferation and migration by targeting β-TrCP2显示文摘胰腺的癌症是好攻击的恶意。中部的幸存率仍然保持低,显示新奇 biomarkers 和治疗学的目标的鉴定是批评的。这里,我们在胰腺的癌症开发检验了 microRNA-182 (miR-182 ) 的角色。人的胰腺的癌症标本和房间线的分析证明 miR-182 是在胰腺的癌症的 overexpressed 并且支持肿瘤增长和侵略。-TrCP2 作为 miR-182 的一个直接目标被证实。-TrCP2 的 Silencing 增加了类似于 miR-182 overexpression 的 -catenin, 的层次。-TrCP2 的宫外的表示禁止了 -catenin 发信号的 miR-182-induced 激活。由 -TrCP2 的 miR-182 和它的颠倒的 oncogenic 效果在 vivo 被证实。这研究建议 -TrCP 和 miR-182 可以为胰腺的癌症的早察觉和治疗是可能的 biomarkers 和目标。 | Shi Wang Jiansong Ji Jingjing Song Xue Li Shilong Han Weishuai Lian Chuanwu Cao Xiaoping Zhang Maoquan Li | 2016 | Acta Biochimica et Biophysica Sinica2016，48，12： | 4 |
| 6 | Green tea epigallocatechin-3-gallate (EGCG) promotes neural progenitor cell proliferation and sonic hedgehog pathway activation during adult hippocampal neurogenesis显示文摘 | Yanyan Wang Maoquan Li Xueqing Xu Min Song Huansheng Tao Yun Bai | | 0，，08： | 1 |
| 7 | On regularity of G-rnorphic rings显示文摘 | LI Yanwu CHU Maoquan | 2007 | Journal of Anhui Normal University(Natural Science)2007，30，5： | 1 |
| 8 | Clinical practice guidelines for the interventional treatment of advanced pancreatic cancer(5^(th)edition)显示文摘Pancreatic cancer has become a major disease affecting people’s health because of its insidiousness,rapid progression and poor prognosis.Based on the practical needs of clinical work,combined with domestic multi-center research and experience,this guideline provides constructive suggestions for the interventional treatment of pancreatic cancer. | Maoquan Li | 2021 | Journal of Interventional Medicine2021，4，4： | 1 |
| 9 | Growth of BiOBr/ZIF‑67 Nanocomposites on Carbon Fiber Cloth as Filter‑Membrane‑Shaped Photocatalyst for Degrading Pollutants in Flowing Wastewater显示文摘BiOBr-based nanocomposite photocatalysts are used for removing the organic pollutants,but their poor adsorption/photocatalytic performances and the low potential for recycling limit their application.To solve the issue,herein we report a large-area recyclable CFC/BiOBr/ZIF-67 filter-membrane-shaped photocatalyst prepared by in situ growth of BiOBr/ZIF-67 nanocomposites on carbon fiber cloth(CFC).Fabrication process is based on hydrothermal synthesis of BiOBr nanosheets(diameter 0.5–1μm)on carbon fiber cloth(as substrate material)and then a chemical bath route is used to grow ZIF-67 nanoparticles(diameter 300–600 nm)in situ on the surface of CFC/BiOBr.Resulted composite,CFC/BiOBr/ZIF-67,exhibits a high specific surface area(545.82 m^(2) g^(−1))and a wide photoabsorption,accompanied by an absorption edge(~620 nm).In dark condition,CFC/BiOBr/ZIF-67 adsorbs bisphenol A(BPA)and orange 7(AO7)within 60 min,respectively with 20.0% and 40.1% efficiency.This level of efficiencies are correspondingly 2.6 and 3.2 times more that of the bare CFC/BiOBr(7.6%for BPA and 12.4% for AO7).Under visible light irradiation,CFC/BiOBr/ZIF-67 can degrade 69.7% of BPA and 96.0% of AO7,in 120 min,which are,respectively,1.3 and 1.8 times higher than the absorption efficiency of bare CFC/BiOBr(53.2% for BPA,52.0% for AO7).When CFC/BiOBr/ZIF-67 is used as a filter membrane for photocatalytic removal of pollutants in flowing wastewater(AO7,rate:~1.5 L h^(−1)),92.2%of AO7 can be decomposed after 10 filtering cycles.This study suggests CFC/BiOBr/ZIF-67 as a novel highly functional,recyclable and environmental friendly photo-driven membrane filter for purification and recovery of flowing surface waste waters. | Xiaolong Li Ting Liu Yan Zhang Jiafeng Cai Mengqiang He Maoquan Li Zhigang Chen Lisha Zhang | 2022 | Advanced Fiber Materials2022，4，6： | 1 |
| 10 | The polycaprolactone/silk fibroin/carbonate hydroxyapatite electrospun scaffold promotes bone reconstruction by regulating the polarization of macrophages显示文摘Macrophages are known to modulate the osteogenic environment of bone regeneration elicited by biological bone grafts.Alteration in certain chemical components tends to affect macrophages polarization.Comparatively to hydroxyapatite(HAp),carbonate hydroxyapatite(CHA)consists of 7.4(wt%)carbonate ions and more closely resembles the mineral content of bone.It remains unknown whether CHA scaffolds or HA scaffolds have better osteogenic properties.In this study,we fabricated PCL/SF scaffold,PCL/SF/HAp scaffold and PCL/SF/CHA scaffold using the electrospinning technique.Despite comparable mechanical properties,the PCL/SF/CHA scaffold exhibited better osteogenic properties than the PCL/SF/HAp scaffold.Although no significant differences were observed between the two scaffolds for promoting osteoblast differentiation in vitro,the PCL/SF/CHA group appeared to be more effective at promoting bone regeneration in cranial defects in vivo.The PCL/SF/CHA scaffold was found to promote macrophage polarization toward M2 via activating the JAK/STAT5 pathway which caused a pro-osteogenic microenvironment to facilitate osteoblast differentiation.The results of this study indicated a higher potential of CHA to substitute HAp in the production of bone scaffolds for better bone regeneration. | Xiaoshi Jia Jing Zhou Jinqiu Ning Maoquan Li Yitong Yao Xiaodong Wang Yutao Jian Ke Zhao | 2022 | Regenerative Biomaterials2022，9，1： | 0 |
| 11 | The Research of Fine Arts Education with Cultivating Aesthetic Appreciation and Creativity as the Core Quality显示文摘In the process of art education and teaching,the improvement of students'aesthetic quality and creative ability is the main goal of art teaching.At the same time,it is also the fundamental basis and direction of teaching.In art teaching,aesthetic education is not only reflected by art skills and teaching basis,but also can not separate aesthetic education from basic knowledge and skills of art education.The cultivation of students'artistic quality and creative ability is the main content of the current development of art education and teaching.Colleges and universities should effectively improve and optimize the teaching courses and teaching contents from the aspects of teaching methods,teaching environment,teaching resources,so as to realize the effective cultivation of students'aesthetic quality and creativity.This paper mainly expounds the significance of cultivating students'aesthetic quality and creative ability,and effectively expounds the methods of improvement,aiming to promote the continuous development and reform of art education in colleges and universities,and improve the level of art education. | Maoquan Li | 2021 | Journal of Contemporary Educational Research2021，5，2： | 0 |
| 12 | Oxygen and hydrogen peroxide self-supplying magnetic nanoenzymes for cancer therapy through magneto-mechanical force,force-induced reactive oxygen species,chemodynamic effects,and cytotoxicity of Ca^(2+) ions显示文摘The use of magneto-mechanical force to kill cancer cells has attracted significant attention in recent years.However,many reports have focused on in vitro experiments with a single treatment.Herein,CaO_(2)-coated Fe_(3)O_(4)core–shell magnetic nanoenzymes(Fe_(3)O_(4)/CaO_(2))are developed for low frequency vibrating magnetic field(VMF)-induced multimodal cancer therapy.Fe_(3)O_(4)/CaO_(2)are shown to efficiently generate O_(2),H_(2)O_(2),·OH through hydrolysis of CaO_(2)and a CaO_(2)-strengthened Fenton reaction,killing laryngeal carcinoma cells and inhibiting mouse tumor growth(chemodynamic therapy(CDT)).Both Fe_(3)O_(4)and Fe_(3)O_(4)/CaO_(2)triggered by a VMF are shown to damage the cytoskeleton of cancer cells through magneto-mechanical force(maxima:223 piconewtons or larger by Fe_(3)O_(4)/CaO_(2)aggregations)and induce the generation of intracellular reactive oxygen species(ROS),the VMF-triggered Fe_(3)O_(4)/CaO_(2)is shown to generate additional intracellular ROS.Upon exposure to a VMF,the cell killing efficiency and tumor growth inhibition were further significantly improved by Fe_(3)O_(4)/CaO_(2)through CDT,magnetomechanical force,force-induced ROS,the cytotoxicity of Ca^(2+)ions.In addition,the Fe_(3)O_(4)/CaO_(2)nanoenzymes and VMFinduced treatment are shown to be safe for mice.The results of this study open the door for treating solid tumors without inducing multidrug resistance through the combination of CDT and force. | Jie Liu Wenxuan Yang Yuqiao Huang Ji Li Chunjiao Zhu Guangjin Pu Bo Wang Xin Gui Maoquan Chu | 2023 | Nano Research2023，16，5： | 0 |
| 13 | Exogenous FGF-2 improves biological activity of endothelial progenitor cells exposed to high glucose conditions显示文摘Purpose: To investigate the effects of exogenous basic fibroblast growth factor-2(FGF-2) on the biological activity of endothelial progenitor cells(EPCs) exposed to high glucose conditions. Materials and Methods: 1) Bone marrow EPCs from C57BL/6 mice were isolated and cultured in vitro. EPC purity was identified by flow cytometry and immunofluorescence staining. 2) Apoptosis was detected by TUNEL assay. Migration and tube formation ability was detected by Transwell chamber and Matrigel assays, respectively. The expression and activation of β-catenin was detected by Western blot. 3) Doppler flowmetry was used to detect the effect of FGF2 on blood flow recovery in ischemic hind limbs of mice. Results: 1) FGF-2 treatment reversed high glucose induced growth inhibition of EPCs. FGF-2 treatment also increased migration and tube formation ability of EPCs even in high glucose conditions. 2) Western blot analysis demonstrated that the percentage of activated β-catenin/total β-catenin in the high glucose group were significantly lower than that in the control group, while FGF-2 treatment reversed high glucose induced β-catenin inhibition. 3) In vivo experiments demonstrated that the blood flow recovery in ischemic hind limbs of mice was significantly improved after FGF-2 treatment. Conclusion: Exogenous FGF-2 could play a role in the functional repair of damaged EPC exposed to high glucose conditions, via the activation of the Wnt/β-catenin signaling pathway. | Yang Li Xue Li Shilong Han Weishuai Lian Jie Cheng Xiaoyun Xie Maoquan Li | 2018 | Journal of Interventional Medicine2018，1，1： | 0 |
| 14 | Controllable Polymerization of N-Substituted β-Alanine N-Thiocarboxyanhydrides for Convenient Synthesis of Functional Poly(β-peptoid)s显示文摘Poly(β-peptoid)is a class of polypeptide mimics that possesses excellent biocompatibility and resistance to proteolysis.However,the synthesis of poly(β-peptoid)s with functionalities is a long-standing challenge that greatly hinders the functional study and application of poly(β-peptoid)s.We report a controllable and easy synthesis of poly(β-peptoid)s bearing diverse functionalities via the ring-opening polymerization on N-substitutedβ-alanine N-thiocarboxyanhydrides(β-NNTAs).The polymerization can be carried out in openvesselsundermildconditions usingaminesas the initiators to provide poly(β-peptoid)s with targeted molecular weights,narrow dispersities,and diverse functionalities in the side chains and termini.Theβ-NNTAs polymerization is even compatible with initiators bearing unprotected hydroxyl groups.The amphiphilic/cationic poly(β-peptoid)s exhibit a broad spectrum and potent antibacterial activities against multidrug-resistant bacteria.In addition,the highly favored stability ofβ-NNTAmonomers for purification and storage highlights the advantages of thisβ-NNTA polymerization strategy for poly(β-peptoid)s synthesis,functional study,and application as a synthetic mimic of polypeptides. | Ximian Xiao Min Zhou Zihao Cong Longqiang Liu Jingcheng Zou Zhemin Ji Ruxin Cui Yueming Wu Haodong Zhang Sheng Chen Maoquan Li Runhui Liu | 2023 | CCS Chemistry2023，5，4： | 0 |
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