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| 1 | Bone-conditioned medium contributes to initiation and progression of osteogenesis by exhibiting synergistic TGF-β1/BMP-2 activity显示文摘Guided bone regeneration (GBR) often utilizes a combination of autologous bone grafts, deproteinized bovine bone mineral(DBBM), and collagen membranes. DBBM and collagen membranes pre-coated with bone-conditioned medium (BCM) extracted from locally harvested autologous bone chips have shown great regenerative potential in GBR. However, the underlying molecular mechanism remains largely unknown. Here, we investigated the composition of BCM and its activity on the osteogenic potential of mesenchymal stromal cells. We detected a fast and significant (P <0.001) release of transforming growth factor-β1 (TGF-β1) from autologous bone within 10 min versus a delayed bone morphogenetic protein-2 (BMP-2) release from 40 min onwards. BCMs harvested within short time periods (10, 20, or 40 min), corresponding to the time of a typical surgical procedure, significantly increased the proliferative activity and collagen matrix production of BCM-treated cells. Long-term (1, 3, or 6 days)-extracted BCMs promoted the later stages of osteoblast differentiation and maturation. Short-term-extracted BCMs, in which TGF-β1 but no BMP-2was detected, reduced the expression of the late differentiation marker osteocalcin. However, when both growth factors were present simultaneously in the BCM, no inhibitory effects on osteoblast differentiation were observed, suggesting a synergistic TGF-β1/BMP-2 activity. Consequently, in cells that were co-stimulated with recombinant TGF-β1 and BMP-2, we showed a significant stimulatory and dose-dependent effect of TGF-β1 on BMP-2-induced osteoblast differentiation due to prolonged BMP signaling and reduced expression of the BMP-2 antagonist noggin. Altogether, our data provide new insights into the molecular mechanisms underlying the favorable outcome from GBR procedures using BCM, derived from autologous bone grafts. | Maria B.Asparuhova Jordi Caballé-Serrano Daniel Buser Vivianne Chappuis | 2018 | International Journal of Oral Science2018，10，4： | 17 |
| 2 | Excretion of biliary compounds during intrauterine life显示文摘In adults,the hepatobiliary system,together with the kidney,constitute the main routes for the elimination of several endogenous and xenobiotic compounds into bile and urine,respectively.However,during intrauterine life the biliary route of excretion for cholephilic compounds,such as bile acids and biliary pigments,is very poor.Although very early in pregnancy the fetal liver produces bile acids,bilirubin and biliverdin,these compounds cannot be efficiently eliminated by the fetal hepatobiliary system,owing to the immaturity of the excretory machinery in the fetal liver.Therefore,the potentially harmful accumulation of cholephilic compounds in the fetus is prevented by their elimination across the placenta.Owing to the presence of detoxifying enzymes and specifi c transport systems at different locations of the placental barrier,such as the endothe-lial cells of chorionic vessels and trophoblast cells,this organ plays an important role in the hepatobiliary-like function during intrauterine life.The relevance of this excretory function in normal fetal physiology is evident in situations where high concentrations of biliary compounds are accumulated in the mother.This may result in oxidative stress and apoptosis,mainly in the placenta and fetal liver,which might affect normal fetal development and challenge the fate of the pregnancy.The present article reviews current knowledge of the mechanisms underlying the hepatobiliary function of the fetal-placental unit and the repercussions of several pathological conditions on this tandem. | Rocio IR Macias Jose JG Marin Maria A Serrano | 2009 | World Journal of Gastroenterology2009，15，7： | 6 |
| 3 | The Hallmarks of Aging显示文摘 | Carlos López-Otín Maria A. Blasco Linda Partridge Manuel Serrano Guido Kroemer | 2013 | Cell2013，，6： | 5 |
| 4 | The role of reduced intracellular concentrations of active drugs in the lack of response to anticancer chemotherapy显示文摘 | Jose JG MARIN Maria J MONTE Alba G BLAZQUEZ Rocio IR MACIAS Maria A SERRANO Oscar BRIZ | 2014 | Acta Pharmacologica Sinica2014，35，1： | 4 |
| 5 | Anti- Helicobacter pylori activity of Terminalia macroptera root显示文摘 | Olga Silva Sílvia Viegas Cristina de Mello-Sampayo Maria Jo?o P. Costa Rita Serrano José Cabrita Elsa T. Gomes | 2012 | Fitoterapia2012，，5： | 2 |
| 6 | Bioethanol, biohydrogen and biogas production from wheat straw in a biorefinery concept 显示文摘 | PRASAD KAPARAJU MARIA SERRANO ANNE BELINDA THOMSEN | 2009 | Bioresource Technology2009，100，9： | 1 |
| 7 | Thermodynamic representation of the solubility for potassium dihydrogen phosphate (KDP) + water + alcohols systems显示文摘 | Paulo A Barata Maria L Serrano | 1997 | Fluid Phase Equilibria1997，141，12： | 1 |
| 8 | Effect of artemisinin/artesunate as inhibitors of hepatitis B virus production in an “in vitro” replicative system显示文摘 | Marta R. Romero Thomas Efferth Maria A. Serrano Beatriz Casta?o Rocio I.R. Macias Oscar Briz Jose J.G. Marin | 2005 | Antiviral Research2005，，2： | 1 |
| 9 | Effect of reactor configuration on biogas production from wheat straw hydrolysate显示文摘 | Kaparaju Prasad Serrano Maria Angelidaki Irini | 2009 | Bioresource Technology2009，100，24： | 1 |
| 10 | Telomeres Acquire Embryonic Stem Cell Characteristics in Induced Pluripotent Stem Cells显示文摘 | Rosa M. Marion Katerina Strati Han Li Agueda Tejera Stefan Schoeftner Sagrario Ortega Manuel Serrano Maria A. Blasco | 2009 | Cell Stem Cell2009，，2： | 1 |
| 11 | Response of transcription factorNFATc3 to excitotoxic and traumatic brain insults:Identification of asubpopulation of reactive astrocytes显示文摘 | Maria C Pedro T Serrano P | 2011 | GLIA2011，59，： | 1 |
| 12 | Thl and Th2 lymphocytes in perennialallergic rhinitis显示文摘 | Daniel Aguilar Angeles Ernestina Serrano Miranda Maria Isabel Rojo Gutierrez | 2006 | Rev Alerg Mex2006，53，3： | 1 |
| 13 | The Addition of Essential Oils to MAP as a Tool to Maintain the Overall Quality of Fruits显示文摘 | Maria Serrano | 2008 | Trends in Food Science &Technology2008，19，9： | 1 |
| 14 | Effects of grape antioxidant dietary fiber in cardiovascular disease risk factors显示文摘 | Jara Pérez Jiménez Jose Serrano Maria Tabernero Sara Arranz M. Elena Díaz-Rubio Luis García-Diz Isabel Go?i Fulgencio Saura-Calixto | 2008 | Nutrition2008，，7： | 1 |
| 15 | Role of calcium and heat treatments in alleviating physiological changes induced by mechanical damage in plum显示文摘 | Maria Serrano Domingo Martinez-Romero Salvador Castillo | 2004 | Postharvest Biology and Technology2004，34，： | 1 |
| 16 | Roles of notch signalling in mandibular condylar cartilage显示文摘 | Maria J. Serrano Sarah So Robert J. Hinton | 2014 | Archives of Oral Biology2014，，： | 1 |
| 17 | Bis(salicylaldiminate) copper(II) and palladium(II) complexes: towards columnar mesophases显示文摘 | Joaquin Barbera Raquel Gimenez Nelida Gimeno Mercedes Marcos Maria Del Carmen Pina Jose Luis Serrano | 2003 | Liquid Crystals2003，，6： | 1 |
| 18 | Effects of grape antioxidant dietary fiber in cardiovascular disease risk factors显示文摘 | Jara Perez-Jimenez Jose Serrano Maria Tabernero | 2008 | Nutrition2008，，24： | 1 |
| 19 | Cellular Senescence in Cancer and Aging显示文摘 | Manuel Collado Maria A. Blasco Manuel Serrano | 2007 | Cell2007，，2： | 1 |
| 20 | Polyamines and ethylene changes during germination of different plant species under salinity显示文摘 | Pedro J Zapata Maria Serrano Pretel M T | 2004 | Plant Science2004，167，： | 1 |
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