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| 1 | Genome and transcriptome analysis of the grapevine(Vitis vinifera L.) WRKY gene family显示文摘The plant WRKY gene family represents an ancient and complex class of zinc-finger transcription factors(TFs)that are involved in the regulation of various physiological processes,such as development and senescence,and in plant response to many biotic and abiotic stresses.Despite the growing number of studies on the genomic organisation of WRKY gene family in different species,little information is available about this family in grapevine(Vitis vinifera L.).In the present study,a total number of 59 putative grapevine WRKY transcription factors(VvWRKYs)were identified based on the analysis of various genomic and proteomic grapevine databases.According to their structural and phylogentic features,the identified grapevine WRKY transcription factors were classified into three main groups.In order to shed light into their regulatory roles in growth and development as well as in response to biotic and abiotic stress in grapevine,the VvWRKYs expression profiles were examined in publicly available microarray data.Bioinformatics analysis of these data revealed distinct temporal and spatial expression patterns of VvWRKYs in various tissues,organs and developmental stages,as well as in response to biotic and abiotic stresses.To also extend our analysis to situations not covered by the arrays and to validate our results,the expression profiles of selected VvWRKYs in response to drought stress,Erysiphe necator(powdery mildew)infection,and hormone treatments(salicilic acid and ethylene),were investigated by quantitative real-time reverse transcription PCR(qRT-PCR).The present study provides a foundation for further comparative genomics and functional studies of this important class of transcriptional regulators in grapevine. | Min Wang Alessandro Vannozzi Gang Wang Ying-Hai Liang Giovanni Battista Tornielli Sara Zenoni Erika Cavallini Mario Pezzotti Zong-Ming(Max)Cheng | 2014 | Horticulture Research2014，1，1： | 19 |
| 2 | Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development显示文摘The Dof(DNA-binding with one finger)protein family spans a group of plant transcription factors involved in the regulation of several functions,such as plant responses to stress,hormones and light,phytochrome signaling and seed germination.Here we describe the Dof-like gene family in grapevine(Vitis vinifera L.),which consists of 25 genes coding for Dof.An extensive in silico characterization of the VviDofL gene family was performed.Additionally,the expression of the entire gene family was assessed in 54 grapevine tissues and organs using an integrated approach with microarray(cv Corvina)and real-time PCR(cv Pinot Noir)analyses.The phylogenetic analysis comparing grapevine sequences with those of Arabidopsis,tomato,poplar and already described Dof genes in other species allowed us to identify several duplicated genes.The diversification of grapevine DofL genes during evolution likely resulted in a broader range of biological roles.Furthermore,distinct expression patterns were identified between samples analyzed,corroborating such hypothesis.Our expression results indicate that several VviDofL genes perform their functional roles mainly during flower,berry and seed development,highlighting their importance for grapevine growth and production.The identification of similar expression profiles between both approaches strongly suggests that these genes have important regulatory roles that are evolutionally conserved between grapevine cvs Corvina and Pinot Noir. | Danielle Costenaro da Silva Vítor da Silveira Falavigna Marianna Fasoli Vanessa Buffon Diogo Denardi Porto Georgios Joannis Pappas Jr Mario Pezzotti Giancarlo Pasquali Luís Fernando Revers | 2016 | Horticulture Research2016，3，1： | 5 |
| 3 | Pollination modulates expression of the PPAL gene, a pistil-specific β-expansin显示文摘 | Mario Pezzotti Richard Feron Celestina Mariani | 2002 | Plant Molecular Biology2002，，2： | 1 |
| 4 | Pollination modulates expression of the PPAL gene, a pistil-specific β-expansin显示文摘 | Mario Pezzotti Richard Feron Celestina Mariani | 2002 | Plant Molecular Biology2002，，2： | 1 |
| 5 | The SSR-based molecular profile of 1005 grapevine ( Vitis vinifera L.) accessions uncovers new synonymy and parentages, and reveals a large admixture amongst varieties of different geographic origin显示文摘 | Guido Cipriani Alessandro Spadotto Irena Jurman Gabriele Di Gaspero Manna Crespan Stefano Meneghetti Enrica Frare Rita Vignani Mauro Cresti Michele Morgante Mario Pezzotti Enrico Pe Alberto Policriti Raffaele Testolin | 2010 | Theoretical and Applied Genetics2010，，8： | 1 |
| 6 | A molecular phenology scale of grape berry development显示文摘Fruit growth and development consist of a continuous succession of physical,biochemical,and physiological changes driven by a genetic program that dynamically responds to environmental cues.Establishing recognizable stages over the whole fruit lifetime represents a fundamental requirement for research and fruit crop cultivation.This is especially relevant in perennial crops like grapevine(Vitis vinifera L.)to scale the development of its fruit across genotypes and growing conditions.In this work,molecular-based information from several grape berry transcriptomic datasets was exploited to build a molecular phenology scale(MPhS)and to map the ontogenic development of the fruit.The proposed statistical pipeline consisted of an unsupervised learning procedure yielding an innovative combination of semiparametric,smoothing,and dimensionality reduction tools.The transcriptomic distance between fruit samples was precisely quantified by means of the MPhS that also enabled to highlight the complex dynamics of the transcriptional program over berry development through the calculation of the rate of variation of MPhS stages by time.The MPhS allowed the alignment of time-series fruit samples proving to be a complementary method for mapping the progression of grape berry development with higher detail compared to classic time-or phenotype-based approaches. | Giovanni Battista Tornielli Marco Sandri Marianna Fasoli Alessandra Amato Mario Pezzotti Paola Zuccolotto Sara Zenoni | 2023 | Horticulture Research2023，10，5： | 0 |
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