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| 1 | Genome and transcriptome analysis of the grapevine(Vitis vinifera L.) WRKY gene family显示文摘The plant WRKY gene family represents an ancient and complex class of zinc-finger transcription factors(TFs)that are involved in the regulation of various physiological processes,such as development and senescence,and in plant response to many biotic and abiotic stresses.Despite the growing number of studies on the genomic organisation of WRKY gene family in different species,little information is available about this family in grapevine(Vitis vinifera L.).In the present study,a total number of 59 putative grapevine WRKY transcription factors(VvWRKYs)were identified based on the analysis of various genomic and proteomic grapevine databases.According to their structural and phylogentic features,the identified grapevine WRKY transcription factors were classified into three main groups.In order to shed light into their regulatory roles in growth and development as well as in response to biotic and abiotic stress in grapevine,the VvWRKYs expression profiles were examined in publicly available microarray data.Bioinformatics analysis of these data revealed distinct temporal and spatial expression patterns of VvWRKYs in various tissues,organs and developmental stages,as well as in response to biotic and abiotic stresses.To also extend our analysis to situations not covered by the arrays and to validate our results,the expression profiles of selected VvWRKYs in response to drought stress,Erysiphe necator(powdery mildew)infection,and hormone treatments(salicilic acid and ethylene),were investigated by quantitative real-time reverse transcription PCR(qRT-PCR).The present study provides a foundation for further comparative genomics and functional studies of this important class of transcriptional regulators in grapevine. | Min Wang Alessandro Vannozzi Gang Wang Ying-Hai Liang Giovanni Battista Tornielli Sara Zenoni Erika Cavallini Mario Pezzotti Zong-Ming(Max)Cheng | 2014 | Horticulture Research2014，1，1： | 19 |
| 2 | Chronic constipation in hemiplegic patients显示文摘AIM: To assess the prevalence of bowel dysfunction in hemiplegic patients, and its relationship with the site of neurological lesion, physical immobilization and pharmacotherapy. METHODS: Ninety consecutive hemiplegic patients and 81 consecutive orthopedic patients were investigated during physical motor rehabilitation in the same period, in the same center and on the same diet. All subjects were interviewed ≥ 3 mo after injury using a questionnaire inquiring about bowel habits before injury and at the time of the interview. Patients’ mobility was evaluated by the Adapted Patient Evaluation Conference System. Drugs considered for the analysis were nitrates, angiogenic converting enzyme (ACE) inhibitors, calcium antagonists, anticoagulants, antithrombotics, antidepressants, anti-epileptics. RESULTS: Mobility scores were similar in the two groups. De novo constipation (OR = 5.36) was a frequent outcome of the neurological accident. Hemiplegics showed an increased risk of straining at stool (OR: 4.33), reduced call to evacuate (OR: 4.13), sensation of incomplete evacuation (OR: 3.69), use of laxatives (OR: 3.75). Logistic regression model showed that constipation was significantly and independently associated with hemiplegia. A positive association was found between constipation and use of nitrates and antithrombotics in both groups. Constipation was not related to the site of brain injury. CONCLUSION: Chronic constipation is a possible outcome of cerebrovascular accidents occurring in 30% of neurologically stabilized hemiplegic patients. Its onset after a cerebrovascular accident appears to be independent from the injured brain hemisphere, and unrelated to physical inactivity. Pharmacological treatment with nitrates and antithrombotics may represent an independent risk factor for developing chronic constipation. | F Bracci D Badiali P Pezzotti G Scivoletto U Fuoco L Di Lucente A Petrelli E Corazziari | 2007 | World Journal of Gastroenterology2007，13，29： | 15 |
| 3 | Relationship between antral distension and postprandial symptoms in functional dyspepsia显示文摘AIM: To investigate in patients with functional dyspepsia (FD) after an every-day meal whether (1) gastrointestinal (GI) and extra-GI symptoms had any relation with the degree of antral volume, (2) the onset of postprandial symptoms was associated with, and may predict, delayed gastric emptying. METHODS: In 94 symptomatic FD patients, antral volume variations and gastric emptying were assessed with ultrasonography after a 1050 kcal meal. Symptoms were evaluated with a standardized questionnaire. The association of GI and extra-GI symptoms with antral volumes and gastric emptying were estimated with logistic regression analysis. RESULTS: Forty percent of patients did not report any symptoms after a meal. Compared to the healthy controls, the antrum was more distended in patients throughout the entire observation period and 37 (39.4%) patients had delayed gastric emptying. Only postprandial drowsiness was associated with antral volume variations (AOR = 1.42; P < 0.001) and with delayed gastric emptying (AOR = 3.59; P < 0.03). CONCLUSION: In FD patients, GI symptoms are neither associated with antral distension nor with gastric emptying. Drowsiness is associated with antral distension and delayed gastric emptying. The onset of drowsiness is preceded by an increment of antral distension and the duration of the symptom appears to be related to the persistence of antral distension. | Nadia Pallotta Patrizio Pezzotti Enrico Corazziari | 2006 | World Journal of Gastroenterology2006，12，43： | 7 |
| 4 | Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development显示文摘The Dof(DNA-binding with one finger)protein family spans a group of plant transcription factors involved in the regulation of several functions,such as plant responses to stress,hormones and light,phytochrome signaling and seed germination.Here we describe the Dof-like gene family in grapevine(Vitis vinifera L.),which consists of 25 genes coding for Dof.An extensive in silico characterization of the VviDofL gene family was performed.Additionally,the expression of the entire gene family was assessed in 54 grapevine tissues and organs using an integrated approach with microarray(cv Corvina)and real-time PCR(cv Pinot Noir)analyses.The phylogenetic analysis comparing grapevine sequences with those of Arabidopsis,tomato,poplar and already described Dof genes in other species allowed us to identify several duplicated genes.The diversification of grapevine DofL genes during evolution likely resulted in a broader range of biological roles.Furthermore,distinct expression patterns were identified between samples analyzed,corroborating such hypothesis.Our expression results indicate that several VviDofL genes perform their functional roles mainly during flower,berry and seed development,highlighting their importance for grapevine growth and production.The identification of similar expression profiles between both approaches strongly suggests that these genes have important regulatory roles that are evolutionally conserved between grapevine cvs Corvina and Pinot Noir. | Danielle Costenaro da Silva Vítor da Silveira Falavigna Marianna Fasoli Vanessa Buffon Diogo Denardi Porto Georgios Joannis Pappas Jr Mario Pezzotti Giancarlo Pasquali Luís Fernando Revers | 2016 | Horticulture Research2016，3，1： | 5 |
| 5 | Relationship between gastrointestinal and extra-gastrointestinal symptoms and delayed gastric emptying in functional dyspeptic patients显示文摘AIM: Delayed gastric emptying and an enlarged fasting gastric antrum are common findings in functional dyspepsia but their relationship with gastrointestinal (GI), and the frequently associated extra-GI symptoms remains unclear.This study evaluated the relationship between GI and extra-GI symptoms, fasting antral volume and delayed gastric emptying in functional dyspepsia.METHODS: In 108 functional dyspeptic patients antral volume and gastric emptying were assessed with ultrasonography (US). Symptoms were assessed with standardized questionnaire. The association of symptoms and fasting antral volume with delayed gastric emptying was estimated with logistic regression analysis.RESULTS: Delayed gastric emptying was detected in 39.8% of the patients. Postprandial drowsiness (AOR 11.25; 95%CI 2.75-45.93), nausea (AOR 3.51; 95%CI 1.19-10.32), fasting antral volume (AOR 1.93; 95%CI 1.22-3.05), were significantly associated with delayed gastric emptying. Symptoms, mainly the extra-GI ones as postprandial drowsiness and nausea, combined with fasting antral volume predicted the modality of gastric emptying with a sensitivity and specificity of 78%.CONCLUSION: In functional dyspeptic patients, (1) an analysis of fasting antral volume and of symptoms can offer valuable indication on the modality of gastric emptying,and (2) it seems appropriate to inquire on postprandial drowsiness that showed the best correlation with delayed gastric emptying. | N Pallotta P Pezzotti E Calabrese F Baccini E Corazziari | 2005 | World Journal of Gastroenterology2005，11，28： | 4 |
| 6 | NGF, DCX, and NSE upregulation correlates with severity and outcome of head trauma in children显示文摘 | A Chiaretti G Barone R Riccardi A Antonelli P Pezzotti O Genovese L Tortorolo G Conti | 2009 | Neurology2009，，7： | 3 |
| 7 | Microstructure and mechanical properties of ZrB 2 –MoSi 2 ceramic composites produced by different sintering techniques显示文摘 | Diletta Sciti Frédéric Monteverde Stefano Guicciardi Giuseppe Pezzotti Alida Bellosi | 2006 | Materials Science & Engineering A2006，，1： | 2 |
| 8 | Microstructure and fracture toughness of Si3N4 ceramics: Combined roles of Grain morphology and secondary phase chemistry 显示文摘 | KLEEBE H J PEZZOTTI G ZIEGLER G | 1999 | Journal of the American Ceramic Society1999，82，7： | 1 |
| 9 | Early and late mortality in elderly patients after hip fracture: a cohort study using administrative health databases in the Lazio re- gion, Italy 显示文摘 | Castronuovo E Pezzotti P Franzo A | 2011 | BMC Geriatr2011，8，： | 1 |
| 10 | Synthesis,thermal behaviour and luminescence properties ofrare earth-doped titania nanofibers 显示文摘 | Cacciotti I Bianco A Pezzotti G | 2011 | Chem EngJ2011，166，2： | 1 |
| 11 | Effect of glass additives on the strength and toughness of polycrystalline alumina显示文摘 | Zhang Y F Pezzotti G | 2002 | J Euro Ceram Soc2002，22，： | 1 |
| 12 | Alzbeimer's amyloid beta-pep- tide (1-42) indoces cell death in bmnan neuroblastoma via Bax/Bel-2 ratio increase:an intriguing role for methionine 35 显示文摘 | Clementi ME Pezzotti M Orsini F | 2006 | Bioehem Biophys Res Cnmmun2006，342，1： | 1 |
| 13 | Enzymatic synthesis of aldonic acids 显示文摘 | Pezzotti F Therisod M | 2006 | Carbohydr Res2006，341，： | 1 |
| 14 | Abrasive wear in ceramic laminated composites显示文摘 | G. de Portu L. Micele D. Prandstraller G. Palombarini G. Pezzotti | 2005 | Wear2005，，9： | 1 |
| 15 | CD4 lymphocyte count as a determinant of the time from HIV seroconversion to AIDS and death from AIDS:evidence from the Italian Seroconversion Study显示文摘 | Phillips AN Pezzotti P Lepri AC | | 0，，9： | 1 |
| 16 | Fracture behavior of hydroxyapatite/polymer interpenetrating network composites prepared by in situ polymerization process显示文摘 | Pezzotti G Asmus S M F | 2001 | Materials Science and Engineering2001，316，： | 1 |
| 17 | Influence of AlF3 and ZnF2 on the phase transformation of gamma to alpha alumina显示文摘 | Yiquan Wu Yufeng Zhang Giuseppe Pezzotti | 2002 | Materials Letters2002，，52： | 1 |
| 18 | Epidemiology and economic burden of herpes zoster and postherpetic neuralgia in Italy:a retrospective,population-based study显示文摘 | Gialloreti LE Merito M Pezzotti P | | 0，，： | 1 |
| 19 | Epideminlogy and eco- nomic burden of herpes zoster mad post-herpetic neuralgia in Italy: a retrospective population-based study显示文摘 | Gialloreti LE Merito M Pezzotti P | 2010 | BMC Infect Dis2010，10，： | 1 |
| 20 | Epidemiology and eco- nomic burden of herpes zoster and post-herpetic neuralgia in Italy : a retrospective, population-based study显示文摘 | Gialloreti LE Merito M Pezzotti P | 2010 | BMC Infect Dis2010，10，： | 1 |
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