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| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | PIK3CA mutations predict recurrence in localized microsatellite stable colon cancer显示文摘 | Manceau G Marisa L Boige V | 2015 | Cancer Med2015，4，3： | 1 |
| 3 | Genotypic differences in polyembryo formation and somatic embryogenesis increment in wheat (Triticum aestivum L.), following 2,4-D treatment显示文摘 | Rosario Linacero Marisa G. López-Bilbao Concepción Romero David A. Laurie Ana M. Vázquez | 1996 | Euphytica1996，，3： | 1 |
| 4 | Promoter and tra- nscription analysis of penicillin-binding protein genes in Streptococcus gordonii 显示文摘 | MARISA H PHILIPPE M VLADIMIR L | 2007 | Antimicrob Agents Chemother2007，51，8： | 1 |
| 5 | Evaluation of the incidence of carboplatin hypersensitivity reactions in cancer patients 显示文摘 | MARISA N ANURADHA K MARTINA L | 2006 | Gynecologic Oncology2006，103，2： | 1 |
| 6 | Preparation and characterisation of ibuprofen-poloxamer 188 granules obtained by melt granulation显示文摘 | Passerini N Albertini B Marisa L | 2002 | Eur J Pharm Sci2002，15，2： | 1 |
| 7 | The human cytomegalovirus显示文摘 | Santo L Marisa G Giorgio G | 2003 | Pharmacology & Therapeutics2003，98，3： | 1 |
| 8 | A refined molecular taxonomy of breast cancer显示文摘 | Guedj M Marisa L de Reynies A | 2012 | Oncogene2012，31，9： | 1 |
| 9 | Extensive character- ization of sphere models established from colorectal canc- er cell lines显示文摘 | Collura A Marisa L Trojan D | 2013 | Cell Mol Life Sci2013，70，4： | 1 |
| 10 | Volatile composition and descriptive sensory analysis of Italian vanilla torrone显示文摘 | MARISA S V2ZQUEZ-ARAI]JO L MINCIONE A | 2010 | International Journal of Food Science and Technology2010，45，： | 1 |
| 11 | A refined molecular taxonomy of breast cancer显示文摘 | Guedj M Marisa L de Reynies A | 2012 | Oncogene2012，31，9： | 1 |
| 12 | A Study of Faculty Data Curation Behaviors and At- titudes at a Teaching-Centered University显示文摘 | Scaramozzino J eanine Marie Ramirez Marisa L McGaughey Karen J | 2012 | College & re search libraries2012，，4： | 1 |
| 13 | Insights into the concept of fish welfare 显示文摘 | Volpatol G L Eliane Goncalves-de-Freitas Marisa emandes- de-Castilho | 2007 | Diseases of Aquatic Organisms Dis Aquat Org2007，75，： | 1 |
| 14 | Extension of the Job Demands-Resources Model in the Prediction of Burnout and Engagement among Teachers over Time显示文摘 | LAURA L P MARISA S S ISABEL M M | 2008 | Psicothema2008，20，3： | 1 |
| 15 | IL-2 and (IFN-gamma),but not IL-4 secretion by peripheral blood mononuclear cells (PBMC) are related to CD4^+ T cells and clinical status in Brazilian HIV-1-infected subjects显示文摘 | Marisa A Hong Valeria L Wakim Simone J Salomao | 1998 | Revista do Instituto de Medicina Tropical de Sao Paulo1998，40，6： | 1 |
| 16 | Real-time PCR for mRNA quantítatíon显示文摘 | Marisa L W Juan F M | | 0，，01： | 1 |
| 17 | Gene expression classification of colon cancer into molecular subtypes:characterization,validation,and prognostic value显示文摘 | MARISA L DE REYNI(E)S A DUVAL A | 2013 | PLoS Med2013，10，10： | 1 |
| 18 | Management Information System:A Tool for Corporate Sustainability显示文摘 | CALDELLIE A MARISA L P | 2004 | Journal of Business Ethics2004，55，： | 1 |
| 19 | Linking transformational leader- ship to nurses' extra-role performance:the mediating role of self-effi cacy and work engagement 显示文摘 | Marisa S Laura L Maria JC | 2011 | J Adv Nur2011，67，10： | 1 |
| 20 | The effect of addition of different elastomers upon the crystalline nature of PP 显示文摘 | Silva A L N Marisa C G Lopes R | 2001 | J Appl Polym Sci2001，81，14： | 1 |
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