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| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | Small intestinal bacterial overgrowth in inactive Crohn's disease:Influence of thiopurine and biological treatment显示文摘AIM:To investigate the influence of thiopurines and biological drugs on the presence of small intestinal bacterial overgrowth(SIBO) in patients with inactive Crohn's disease(CD).METHODS:This was a prospective study in patients with CD in remission and without corticosteroid treatment,included consecutively from 2004 to 2010.SIBO was investigated using the hydrogen glucose breath test.RESULTS:One hundred and seven patients with CD in remission were included.Almost 58%of patients used maintenance immunosuppressant therapy and 19.6%used biological therapy.The prevalence of SIBO was16.8%.No association was observed between SIBO and the use of thiopurine Immunosuppressant(12/62patients),administration of biological drugs(2/21 patients),or with double treatment with an anti-tumor necrosis factor drugs plus thiopurine(1/13 patients).Half of the patients had symptoms that were suggestive of SIBO,though meteorism was the only symptom that was significantly associated with the presence of SIBO on univariate analysis(P<0.05).Multivariate analysis revealed that the presence of meteorism and a fistulizing pattern were associated with the presence of SIBO(P<0.05).CONCLUSION:Immunosuppressants and/or biological drugs do not induce SIBO in inactive CD.Fistulizing disease pattern and meteorism are associated with SIBO. | Cristina Sánchez-Montes Vicente Ortiz Guillermo Bastida Ester Rodríguez María Yago Belén Beltrán Mariam Aguas Marisa Iborra Vicente Garrigues Julio Ponce Pilar Nos | 2014 | World Journal of Gastroenterology2014，20，38： | 4 |
| 3 | Adalimumab in prevention of postoperative recurrence of Crohn's disease in high-risk patients显示文摘AIM:To evaluate the effectiveness of adalimumab in preventing recurrence after intestinal resection for Crohn's disease in high-risk patients.METHODS:A multicenter,prospective,observational study was conducted from June 2009 until June 2010.We consecutively included high-risk Crohn's disease patients who had undergone an ileal/ileocolonic resection.High-risk patients were defined as two or more criteria:smokers,penetrating pattern,one or more previous surgical resections or prior extensive resection.Subcutaneous adalimumab was administered 2 wk(± 5 d) after surgery at a dose of 40 mg eow,with an initial induction dose of 160/80 mg at weeks 0 and 2.Demographic data,previous and concomitant treatments(antibiotics,5-aminosalicylates,corticosteroids,immunomodulators or biologic therapies),smoking status at the time of diagnosis and after the index operation and number of previous resections(type and reason for surgery) were all recorded.Biological status was assessed with C-reactive protein,erythrocyte sedimentation rate and fecal calprotectin.One year(± 3 mo) after surgery,an ileocolonoscopy and/or magnetic resonance enterography was performed.Endoscopic recurrence was defined as Rutgeerts score ≥ i2.Morphological recurrence was based on magnetic resonance(MR) score ≥ MR1.RESULTS:Twenty-nine patients(55.2% males,48.3% smokers at diagnosis and 13.8% after the index operation),mean age 42.3 years and mean duration of the disease 13.8 years were included in the study.A mean of 1.76(range:1-4) resections previous to adalimumab administration and in 37.9% was considered extensive resection.51.7% had previously received infliximab.Immunomodulators were given concomitantly to 17.2% of patients.Four of the 29(13.7%) developed clinical recurrence,6/29(20.7%) endoscopic recurrence and 7/19(36.8%) morphological recurrence after 1-year.All patients with clinical recurrence showed endoscopic and morphological recurrence.A high degree of concordance was found between clinical-endoscopic recurrence(k = 0.76,P < 0.001) and clinical-morphological recurrence(k = 0.63,P = 0.003).Correlation between endoscopic and radiological findings was good(comparing the 5-point Rutgeerts score with the 4-point MR score,a score of i4 was classified as MR3,i3 as MR2,and i2-i1 as MR1)(P < 0.001,r s = 0.825).During follow-up,five(17.2%) patients needed adalimumab dose intensification(40 mg/wk);Mean time to intensification after the introduction of adalimumab treatment was 8 mo(range:5 to 11 mo).In three cases(10.3%),a biological change was needed due to a worsening of the disease after the dose intensification to 40 mg/wk.One patient suffered an adverse event.CONCLUSION:Adalimumab seems to be effective and safe in preventing postoperative recurrence in a selected group of patients who had undergone an intestinal resection for their CD. | Mariam Aguas Guillermo Bastida Elena Cerrillo Belén Beltrán Marisa Iborra Cristina Sánchez-Montes Fernando Muoz Jesús Barrio Sabino Riestra Pilar Nos | 2012 | World Journal of Gastroenterology2012，18，32： | 3 |
| 4 | Influence of the sea rush Juncus martitimus on metal concentration and speciation in estuarine sediment colonized by the plant显示文摘 | Marisa C RAlmeida Ana P Mucha Teresa M S D Vasconce- los | 2004 | Environmental Science and Technology2004，38，： | 1 |
| 5 | Transcripts of a chimeric cDNA of hepatitis C virus genotype lb are infectious in vivo显示文摘 | Masayuki Y Marisa S T Max S | 1998 | Virology1998，244，： | 1 |
| 6 | Parental imprinting of the mouse H19 gene 显示文摘 | Marisa S Bartolomei Sharon Zemel Shirley M Tilghman | 1991 | Nature1991，351，6322： | 1 |
| 7 | Determination of ammonia based on the electro-oxidation of hydroquinone in dimethylformamide or in the room temperature ionic liquid, 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide显示文摘 | Debora Giovanelli Marisa C Buzzeo Nathan S Lawrence Christopher Hardacre Kenneth R Seddon Richard G Compton | 2003 | Talanta2003，，： | 1 |
| 8 | ^sup 18^F-FDG PET After 2 Cycles of ABVD Predicts Event-Free Survival in Early and Advanced Hodgkin Lymphoma显示文摘 | Cerci Juliano J Pracchia Luís F Linardi Camila C G Pitella Felipe A Delbeke Dominique Izaki Marisa Trindade Evelinda Junior José Soares Buccheri Valeria Meneghetti José C | 2010 | The Journal of Nuclear Medicine2010，，： | 1 |
| 9 | Volatile composition and descriptive sensory analysis of Italian vanilla torrone显示文摘 | MARISA S V2ZQUEZ-ARAI]JO L MINCIONE A | 2010 | International Journal of Food Science and Technology2010，45，： | 1 |
| 10 | Temperature effect on the biological activity of Bifidobacterium long- um CRL 849 and LactobaciUus fermentum CRL 251 in pure and mixed cultures grown in soymilk 显示文摘 | MARISA S GARRO GRACIELA F DE VALDEZ GRACIELA S | 2004 | Food Chemistry2004，21，5： | 1 |
| 11 | Dynamic and static adsorption and desorption of Hg(Ⅱ) ions on chitosan membranes and spheres显示文摘 | Rodrigo S Vieira Marisa M Beppu | | 0，，： | 1 |
| 12 | Extension of the Job Demands-Resources Model in the Prediction of Burnout and Engagement among Teachers over Time显示文摘 | LAURA L P MARISA S S ISABEL M M | 2008 | Psicothema2008，20，3： | 1 |
| 13 | Biological activity of Bifidbacterium longum in response to environmental pH显示文摘 | Marisa S Garro Laura Aguirre Graciela Savoy de Giori | 2006 | Applied Microbiology Biotechnology2006，70，： | 1 |
| 14 | Gene expression classification of colon cancer into molecular subtypes:characterization,validation,and prognostic value显示文摘 | MARISA L DE REYNI(E)S A DUVAL A | 2013 | PLoS Med2013，10，10： | 1 |
| 15 | Linking transformational leader- ship to nurses' extra-role performance:the mediating role of self-effi cacy and work engagement 显示文摘 | Marisa S Laura L Maria JC | 2011 | J Adv Nur2011，67，10： | 1 |
| 16 | Flow At Work: Evidence for an upward spiral of personal and organizational resources 显示文摘 | Marisa S Arnold B B | 2006 | J Happiness Stud2006，7，1： | 1 |
| 17 | Volatile composition and descriptive sensory analysis of Italian vanilla torrone显示文摘 | Marisa S Laura V Antonio M et ol | 2010 | Intemafionai Journal of Food Science and Technology2010，45，： | 1 |
| 18 | Lysis ofLaetobacillus casei 5Mn373 accel- erates Grana cheese ripening 显示文摘 | GIANLUIGI S MARISA V | 2005 | Eur Food Res Teehnol2005，220，56： | 1 |
| 19 | Immunogenicity of Hepatitis B vaccine in preterm and full term infants vaccinated within the first week of life显示文摘 | Márcia Soares Freitas da Motta Marisa Márcia Mussi-Pinhata Salim Moysés Jorge Clara Fumiko Tachibana Yoshida Cleonice Barbosa Sandoval de Souza | 2002 | Vaccine2002，，11： | 1 |
| 20 | Optimiza- tion of levofloxacin-loaded crosslinked chitosan microspheres for in- haled aerosol therapy显示文摘 | Marisa C Gaspar Jo Alberto A C C Pais | 2015 | Eur J Pharm Biopharm2015，96，： | 1 |
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