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41篇 您的检索式:作者名="Marushima"
    题名 作者 年代 出处 被引量
1Hepatic reconstruction from fetal porcine liver cells using a radial flow bioreactor显示文摘AIM: To examine the efficacy of the radial flow bioreactor (RFB) as an extracorporeal bioartificial liver (BAL) and the reconstruction of liver organoids using embryonic pig liver cells. METHODS: We reconstructed the liver organoids using embryonic porcine liver cells in the RFB. We also determined the gestational time window for the optimum growth of embryonic porcine liver cells. Five weeks of gestation was designated as embryonic day (E) 35 and 8 wk of gestation was designated as E56. These cells were cultured for one week before morphological and functional examinations. Moreover, the efficacy of pulsed administration of a high concentration hepatocyte growth factor (HGF) was examined. RESULTS: Both cell growth and function were excellent after harvesting on E35. The pulsed administration of a high concentration of HGF promoted the differentiation and maturation of these fetal hepatic cells. Microscopic examination of organoids in the RFB revealed palisading and showed that bile duct-like structures were well developed, indicating that the organoids were mini livers. Transmission electron microscopy revealed microvilli on the luminal surfaces of bile duct-like structures and junctional complexes, which form the basis of the cytoskeleton of epithelial tissues. Furthermore, strong expression of connexin (Cx) 32, which is the main protein of hepatocyte gap junctions, was observed. With respect to liver function, ammonia detoxification and urea synthesis were shown to be performed effectively. CONCLUSION: Our system can potentially be applied in the fields of BAL and transplantation medicine.Yuji Ishii Ryota Saito Hideki Marushima Ryusuke Ito Taro Sakamoto Katsuhiko Yanaga 2008World Journal of Gastroenterology2008,14,17:3
2A subpopulation of endothelial progenitor cells with low aldehyde dehydrogenase activity attenuates acute ischemic brain injury in rats显示文摘Kazuhiro Nakamura Hideo Tsurushima Aiki Marushima Masumi Nagano Toshiharu Yamashita Kensuke Suzuki Osamu Ohneda Akira Matsumura 2012Biochemical and Biophysical Research Communications2012,,1:2
3Pulmonary vein analysis using three- dimensional computed tomography angiography for thoracic surgery显示文摘Akiba T Marushima H Odaka M 2010Gen Thorac Cardiovasc Surg2010,58,7:1
4lmportance of preoperative imaging with 64-row three-dimensional multidetector computed tomography for safer video-assisted thoracic surgery in lung cancer显示文摘Akiba T Marushima H Harada J 2009Surg Today2009,39,10:1
5Anomalous pulmonary vein detected using threedimensional computed tomography in a patient with lung cancer undergoing thoracoscopic lobectomy显示文摘AKIBA T MARUSHIMA H HARADA J 2008Gen Thorac Cardiovasc Surg2008,56,8:1
6Collision free trajectory planning for manipulator using potential funtion显示文摘Mohri A Marushima S Yamarnoto M 1995IEEE International Conference on Robot and Automation1995,,:1
7Anomalous pulmonary vein detected using three-dimensional computed tomography in a patient with lung cancer undergoing thoracoscopic lobectomy显示文摘Akiba T Marushima H Harada J 0,,08:1
8Video-assisted thoracic surgery for recurrent primary spontaneous pneumothorax in reoperated chests显示文摘Akiba T Marushima H Kobayashi S 2009Surg Today2009,39,:1
9Video-assisted thoracoscopic surgery for non-small cell lung cancer in patients on hemodialysis显示文摘Akiba T Marushima H Hiramatsu M 2010Annals of Thoracic and Cardiovascular Surgery2010,16,1:1
10Newly Synthesized Radical Containing Nanoparticles Enhance Neuroprotection After Cerebral Ischemia Reperfusion Injury显示文摘Aiki Marushima Kensuke Suzuki Yukio Nagasaki 2011Neurosurgery2011,68,5:1
11Thoraeoscopic lo- hectomy for treating cancer in a patient with an unusual vein anomaly显示文摘Akiba T Marushima H Kamiya N 2011Ann Thorac Cardiovasc Surg2011,17,5:1
12Brown coal conversion by microwave plasma reactions under successive supply of methane 显示文摘Kamei O Onoe K Marushima W 1998Fuel1998,77,13:1
13Small symptomatic peri- cardial diverticula treated by video-assisted thotacic surgical resec- tion显示文摘Tkina T Marushima H Kobayashi S 2009Ann Thorac Cardiovasc Surg2009,15,5:1
14Subarachnoid hemorrhage not due to ruptured aneurysm in moyamoya disease 显示文摘Marushima A Yanaka K Matsuki T 2006J Clin Neurosci2006,13,:1
15Brown Coal Conversion by Microwave Plasma Reactions under Successive Supply of Methane显示文摘Kamei O Onoe K Marushima W 1998Fuel1998,77,13:1
16Video-assisted thoraco- scopic surgery for non-small cell lung cancer in patients on hemodialysis显示文摘Akiba T Marushima H Hiramatsu M 2010Ann Thorac Cardiovasc Surg2010,16,1:1
17A subpopulation of endothelial progenitor cells with low aldehyde dehydrogenase activity attenuates acute ischemic brain injury in rats 显示文摘Nakamura K Tsurushima H Marushima A 2012Bio- chem Biophys Res Commun2012,418,1:1
18Video-assisted thomcoscopic sur-gery for non-small cell lung cancer in patients oil hemodialysis 显示文摘Akiba Marushima H Hiramatsu M 2010Annals of Thoracic and Cardiovascular Surgery2010,16,1:1
19Enhanced bone formation using hydroxyapatite ceramic coated with fibroblast growth factor-2 显示文摘Tsurushima H Marushima A Suzuki K 2010Acta Biomater2010,6,7:1
20Brown coal conversion by microwave plasma reactions under successive supply of methane 显示文摘Kamei O Onoe K Marushima W 1998Fuel1998,77,13:1
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