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| 1 | miRNA studies in in vitro and in vivo activated hepatic stellate cells显示文摘AIM: To understand which and how different miRNAs are implicated in the process of hepatic stellate cell (HSC) activation. METHODS: We used microarrays to examine the differential expression of miRNAs during in vitro activation of primary HSCs (pHSCs). The transcriptome changes upon stable transfection of rno-miR-146a into an HSC cell line were studied using cDNA microarrays. Selected differentially regulated miRNAs were investigated by quantitative real-time polymerase chain reaction during in vivo HSC activation. The effect of miRNA mimics and inhibitor on the in vitro activation of pHSCs was also evaluated.RESULTS: We found that 16 miRNAs were upregulated and 26 were downregulated significantly in 10-d in vitro activated pHSCs in comparison to quiescent pHSCs. Overexpression of rno-miR-146a was characterized by marked upregulation of tissue inhibitor of metalloproteinase-3, which is implicated in the regulation of tumor necrosis factor-α activity. Differences in the regulation of selected miRNAs were observed comparing in vitro and in vivo HSC activation. Treatment with miR-26a and 29a mimics, and miR-214 inhibitor during in vitro activation of pHSCs induced significant downregulation of collagen type Ⅰ transcription. CONCLUSION: Our results emphasize the different regulation of miRNAs in in vitro and in vivo activated pHSCs. We also showed that miR-26a, 29a and 214 are involved in the regulation of collagen type I mRNA. | Gunter Maubach Michelle Chin Chia Lim Henry Yang | 2011 | World Journal of Gastroenterology2011，17，22： | 12 |
| 2 | GFAP promoter directs lacZ expression specifically in a rat hepatic stellate cell line显示文摘瞄准:GFAP 传统地被认为是为神经神经胶质的一个简历标记(主要星形细胞和 non-myelinating Schwann 房间) 。遗传上,一个 2.2-kb 人 GFAP 倡导者成功地被用来指向星形细胞在试管内和在活体内。更最近, GFAP 也为识别肝的星形细胞(HSC ) 作为几个制造者之一被建立。在这个工程,为指向 HSC 的一样的 2.2-kb 人 GFAP 倡导者的可能的申请被调查。方法:GFAP-lacZ transgene 是进各种各样的房间线(HSC, hepatocyte,和另外的 non-HSC 房间类型) 的 transfected。transgene 表示特性被贝它牛乳糖活动染色的 X 女郎决定。并且 transgene 的应答的海角与典型 pro-fibrotic cytokine TGF-beta1 被测试。内长的 GFAP 基因的表示被即时 RT-PCR 估计,提供为 transgene 表示的一本参考书。结果:结果第一次证明 2.2 kb hGFAP 倡导者不仅能够指导 HSC 特定的表示,而且对已知的 pro-fibrogenic cytokine TGF-beta1 作出回应由在在一个剂量依赖者和时间依赖者举止的规定上面,类似于内长的 GFAP。结论:在结论,这些调查结果为使用 GFAP 倡导者明确地为治疗学的目的操作 HSC 建议了新奇实用程序。 | Gunter Maubach Michelle Chin Chia Lim | 2006 | World Journal of Gastroenterology2006，12，5： | 4 |
| 3 | Impaired activity of thiol-dependent ATPases in rheumatoid mononuclear cell membranes显示文摘 | K. Maubach A. D. Foey Dr. N. D. Hall | 1993 | Agents and Actions1993，，1： | 1 |
| 4 | Expression and up regulation of cathep sin S and other early molecules required for antigen presenta- tion in activated hepatic stellate cells upon IFN-γ treatment 显示文摘 | Maubach G Lin MC Kumar S | 2007 | Biochmi Biophysica Acta2007，1173，2： | 1 |
| 5 | Quantitation of soy-derived phytoestrogens in human breast tissue and biological fluids by high-performance liquid chromatography显示文摘 | MAUBACH J BRACKE M E HEYERICK A | 2003 | J Chromatogr B2003，784，1： | 1 |
| 6 | GABAA receptor subtype selective cognition enhancers显示文摘 | Maubach K | 2003 | Curr Drug Targets CNS Neurol Disord2003，2，： | 1 |
| 7 | TGF-β1 down-regulates connexin 43 expression and gap junction intercellular communication in rat hepatic stellate cells显示文摘 | Lim M C Maubach G Zhuo L | 2009 | Eur J Cell Bio2009，88，12： | 1 |
| 8 | Evaluating sharpness functions for automated scanning electron microscopy显示文摘 | RUDNAYA M E MATTHEIJ R MAUBACH J | 2010 | Journal of Microscopy2010，240，1： | 1 |
| 9 | Quantitation of soy-derived phytoestrogens in human breast tissue and biological fluids by highperformance liquid chromatography显示文摘 | Maubach J Bracke ME Heyerick A | 2003 | J Chromatogr B Anal Technol Biomed Life Sci2003，784，1： | 1 |
| 10 | The success rate of the iliac and femoral veins显示文摘 | Worttzteld A Fink U Maubach P | 1983 | Angiology1983，34，： | 1 |
| 11 | Ca2+/calmodulin‐dependent kinase II contributes to inhibitor of nuclear factor‐kappa B kinase complex activation in Helicobacter pylori infection显示文摘 | Gunter Maubach Olga Sokolova Markus Wolfien Hermann‐Josef Rothk?tter Michael Naumann | 2013 | Cancer2013，，6： | 1 |
| 12 | Chronic substance P ( NK1 ) receptor antagonist and conventional antidepressant treatment increases burst firing of monoamine neurons in the locus coeruleus显示文摘 | Maubach KA Martin K Chicchi G | 2002 | Neuroscience2002，109，3： | 1 |
| 13 | Age-related morphol- ogy of the normal paucreas on computer tomography 显示文摘 | Heuck A Maubach PA Reiser M | 1987 | Gastrointest Radiol1987，12，1： | 1 |
| 14 | Polynomial automorphisms over finite fields显示文摘 | Maubach S | 2001 | Serdica Math2001，27，4： | 1 |
| 15 | An inverse agonist solective for alpha3 subunit-containing GABAA rocoptors onhancoscognition显示文摘 | Dawson GR Maubach KA Collinson N | 2006 | J Pharnmcol Exp Ther2006，316，3： | 1 |
| 16 | Quantitation of soyderived phytoestrogens in human breast tissue and biological fluids by high-performance liquid chromatography 显示文摘 | Maubach J Bracke ME Heyerick A | 2003 | J Chromatogr B Anal yt Technol Biomed Life Sci2003，784，1： | 1 |
| 17 | GABAA receptor subtype selective cognition enhancers显示文摘 | Maubach K | 2003 | Curr Drug Targets CNS Neurol Disord2003，，2： | 1 |
| 18 | The GABAA receptor as a potential target for the treatment of cognitive dysfunction 显示文摘 | Maubach KA | 2006 | Drugs Future2006，31，2： | 1 |
| 19 | I,essons learned from early clinical experience and results of 300 ASR hip resurfacing im- plantations 显示文摘 | Siebel T Maubach S Morlock MM | 2006 | Proc lnst Mech Eng H2006，220，2： | 1 |
| 20 | Expression and upregulation of cathepsin S and other early molecules required for antigen presentation in activated hepatic stellate cells upon IFN-gamma treatment显示文摘 | Maubach G Lim MC Kumar S | 2007 | Biochim Biophys Acta2007，1773，2： | 1 |
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