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| 1 | Essential roles of exosome and circRNA_101093 on ferroptosis desensitization in lung adenocarcinoma显示文摘Background:Resistance to ferroptosis,a regulated cell death caused by irondependent excessive accumulation of lipid peroxides,has recently been linked to lung adenocarcinoma(LUAD).Intracellular antioxidant systems are required for protection against ferroptosis.The purpose of the present studywas to investigate whether and how extracellular system desensitizes LUAD cells to ferroptosis.Methods:Established human lung fibroblasts MRC-5,WI38,and human LUAD H1650,PC9,H1975,H358,A549,and H1299 cell lines,tumor and matched normal adjacent tissues of LUAD,and plasma from healthy individuals and LUAD patients were used in this study.Immunohistochemistry and immunoblotting were used to analyze protein expression,and quantitative reverse transcription-PCR was used to analyze mRNA expression.Cell viability,cell death,and the lipid reactive oxygen species generationwere measured to evaluate the responses to ferroptosis.Exosomes were observed using transmission electron microscope.The localization of arachidonic acid(AA)was detected using click chemistry labeling followed by confocal microscopy.Interactions between RNAs and proteins were detected using RNA pull-down,RNA immunoprecipitation and photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation methods.Proteomic analysis was used to investigate RNA-regulated proteins,and metabolomic analysis was performed to analyze metabolites.Cellderived xenograft,patient-derived xenograft,cell-implanted intrapulmonary LUAD mouse models and plasma/tissue specimens from LUAD patients were used to validate the molecular mechanism.Results:Plasma exosome from LUAD patients specifically reduced lipid peroxidation and desensitized LUAD cells to ferroptosis.A potential explanation is that exosomal circRNA_101093(cir93)maintained an elevation in intracellular cir93 in LUAD to modulate AA,a poly-unsaturated fatty acid critical for ferroptosisassociated increased peroxidation in the plasma membrane.Mechanistically,cir93 interacted with and increased fatty acid-binding protein 3(FABP3),which transported AA and facilitated its reaction with taurine.Thus,global AA was reduced,whereas N-arachidonoyl taurine(NAT,the product of AA and taurine)was induced.Notably,the role of NAT in suppressing AA incorporation into the plasma membrane was also revealed.In pre-clinical in vivo models,reducing exosome improved ferroptosis-based treatment.Conclusion:Exosome and cir93 are essential for desensitizing LUAD cells to ferroptosis,and blocking exosome may be helpful for future LUAD treatment. | Xiao Zhang Yunhua Xu Lifang Ma Keke Yu Yongjie Niu Xin Xu Yi Shi Susu Guo Xiangfei Xue Yikun Wang Shiyu Qiu Jiangtao Cui Hong Wang Xiaoting Tian Yayou Miao Fanyu Meng Yongxia Qiao Yongchun Yu Jiayi Wang | 2022 | Cancer Communications2022，42，4： | 10 |
| 2 | Highly efficient K-doped Mn-Ce catalysts with strong K-Mn-Ce interaction for toluene oxidation显示文摘In this study,K_(x)-Mn-Ce catalysts prepared by sol-gel method were investigated for toluene oxidation.Compared with Mn-Ce,the catalytic performance of K_(x)-Mn-Ce was further improved.X-ray diffraction(XRD),high resolution transmission electron microscopy(HRTEM)and Raman analyses demonstrate that K ions enter the lattice of CeO_(2) and disperse uniformly.The results of X-ray photoelectron spectroscopy(XPS),H_(2)-temperature programmed reduction(H_(2)-TPR).and O_(2)-temperature programmed desorption(O_(2)-TPD)analyses indicate that there is a strong interaction between K,Mn and Ce;the charge co mpensation effect would be induced when K ions enter the lattice of CeO_(2),which leads to more oxygen vacancies due to the generation of more Ce^(3+).Toluene-TPD shows that K-doping enhances the activation ability of toluene.Among all catalysts,K0.1-Mn-Ce shows the highest concentration of Mn^(4+),Ce^(3+),Osur,and redox ability,resulting in higher low-temperature catalytic activity.Additionally,the results of stability and water resistance also prove that K0.1-Mn-Ce catalyst possesses excellent stability and water resistance. | Bin Yang Yiqing Zeng Mingjia Zhang Fanyu Meng Shule Zhang Qin Zhong | 2023 | Journal of Rare Earths2023，41，3： | 2 |
| 3 | Retrospective study of the incidence of HFMD and seroepidemiology of antibodies against EV71 and CoxA16 in prenatal women and their infants 显示文摘 | Zhu Fengcai Liang Zhenglun Meng Fanyue | 2012 | PLoSOne2012，7，37： | 1 |
| 4 | Effect of Shear Wave Velocity on the Ground Motion Parameters of Site Surface显示文摘Based on the data from typical sites in the Jianghuai region,many kinds of soil layer seismic response are modeled by increasing and decreasing the measured values of shear velocity to a certain scale. The seismic response of soil layer sites are calculated using the one-dimensional equivalent linear method in the frequency domain by choosing the Taft,Kobe and El-centro records as the ground motion input. The results show that the impact of shear velocity variability on the surface ground motion is in relation to the soil layer structure and ground motion input parameters such as amplitude and spectral characteristic. With the increase of shear velocity,the PGA( peak ground acceleration)on the surface of site will increase,however,the characteristic period of the acceleration response spectra is decreasing. | Cao Junfeng Feng Weidong Meng Fanyue Dong Shuanglin | 2014 | Earthquake Research in China2014，28，2： | 1 |
| 5 | Synthesis and surface modification of uniform barium borate nanorods for lubrication显示文摘 | Ning Liu Yumei Tian Lianxiang Yu Quanjun Li Fanyu Meng Yunhui Zheng Guangyu Zhang Zhihui Liu Jing Li Fuming Jiang | 2007 | Journal of Alloys and Compounds2007，，1： | 1 |
| 6 | Processing Complex Mixtures of Intact Proteins for Direct Analysis by Mass Spectrometry显示文摘 | Meng Fanyu Cargile Benjamin J Patrie Steven M | 2002 | Anal Chem2002，74，29： | 1 |
| 7 | Synergistic effect of F and triggered oxygen vacancies over F-TiO_(2) on enhancing NO ozonation显示文摘Oxidation-absorption technology is a key step for NOxremoval from low-temperature gas.Under the condition of low O_(3)concentration(O_(3)/NO molar ratio = 0.6), F-TiO_(2)(F-TiO_(2)), which is cheap and environmentally friendly, has been prepared as ozonation catalysts for NO oxidation. Catalytic activity tests performed at 120℃showed that the NO oxidation efficiency of F-TiO_(2)samples was higher than that of TiO_(2)(about 43.7%), and the NO oxidation efficiency of F-TiO_(2)-0.15 was the highest, which was 65.3%. Combined with physicochemical characteristics of catalysts and the analysis of active species, it was found that there was a synergistic effect between F sites and oxygen vacancies on F-TiO_(2), which could accelerate the transformation of monomolecular O_(3)into multi-molecule singlet oxygen(1O_(2)), thus promoting the selective oxidation of NO to NO_(2). The oxidation reaction of NO on F-TiO_(2)-0.15 follows the Eley-Rideal mechanism, that is, gaseous NO reacts with adsorbed O_(3)and finally form NO_(2). | Lina Guo Xu Zhang Fanyu Meng Jing Yuan Yiqing Zeng Chenyang Han Yong Jia Mingyan Gu Shule Zhang Qin Zhong | 2023 | Journal of Environmental Sciences2023，，3： | 0 |
| 8 | Facile Synthesis of Al-pillared Mesoporous Montmorillonite Assisted with Concentrated Al13 Solution显示文摘 | ZHAO Tianqi LI Wenjie WANG Hongyan MENG Fanyu | 2014 | Chemical Research in Chinese Universities2014，30，2： | 0 |
| 9 | Microwave-assisted Synthesis of New 1,2,3-Triazoles Bearing an Isoxazole Ring by the Azide-alkyne Cycloaddition Click Chemistry显示文摘 | LI Jing LIU Hongwei MENG Fanyu YAN Liuqing SHI Yanpeng ZHANG Yumin GU Qiang | 2018 | Chemical Research in Chinese Universities2018，34，2： | 0 |
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