| 1 | Basic helix-loop-helix(bHLH)transcription factor MdbHLH3 negatively affects the storage performance of postharvest apple fruit显示文摘The storage period of fleshy fruits greatly affects their quality and selection,and is largely controlled by genetic factors.Therefore,it is imperative to elucidate how genetic factors affect fruit ripening and its storage.Here,we evaluated the postharvest storage properties of the basic helix-loop-helix(bHLH)transcription factor MdbHLH3-overexpressing transgenic Royal gala apple fruits.During storage,the contents of starch,malic acid,fructose,glucose,and sucrose in fruits of three MdbHLH3 transgenic lines were always higher than those of the wild-type(WT)control.Interestingly,the sugar-acid ratio also showed the same trend during fruit storage.Additionally,the fruit firmness decreased with increasing storage time,and the contents of cell wall components such as water-soluble pectin and cellulose in transgenic fruits were higher than those in control fruits,while the firmness of transgenic fruits was lower than that in WT control fruits.Though the ethylene release rate in both showed the same trend(firstly increasing,then decreasing,and finally peaking)in 90-day stored fruits,transgenic apples had higher ethylene levels than the WT control throughout storage.Furthermore,the activities of membrane peroxidase,antioxidant enzymes,and fruit ripening enzymes in all transgenic fruits were significantly higher than those in the WT control.Thus,our findings show how MdbHLH3 negatively regulates and reduces apple storage time.This may prove useful for not only developing biotechnological strategies,but also support traditional breeding programs,to help improve the storage time of fleshy fruits. | Wenyan Wang Jianqiang Yu Mengchi Du Jiahui Wang Dagang Hu | 2022 | Horticultural Plant Journal2022，8，6： | 2 |
| 2 | Comprehensive evaluation of the optimal rates of irrigation and potassium application for strawberry显示文摘Accurate and effective management of irrigation and fertilization is essential for efficient greenhouse strawberry production.Here,the effects of the combined application of water and potassium on strawberry growth were evaluated by experimenting with 12 treatments,including four transpiration(ET_(c))-based irrigation levels(W1:100%ET_(c),W2:85%ET_(c),W3:70%ET_(c),and W4:55%ET_(c))and three potassium levels according to the target yield(K1:369.5 kg/hm^(2),K2:307.9 kg/hm^(2),and K3:246.3 kg/hm^(2)).Various indexes of strawberry yield,fruit quality,water and fertilizer utilization efficiency,and soil nutrient residues were measured.Irrigation and potassium application had significant effects on strawberry production.Higher irrigation levels increased yield(Y),and moderate water and potassium application increased single fruit weight(SFW),water utilization efficiency(WUE),total soluble sugar(TSSC),and the sugar-acid ratio(SAR).While low-application of potassium increased the soluble protein content(SP),partial factor productivity of potassium(PFP_(K)),and reduce nutrient residues in soil effectively.A comprehensive system for evaluating strawberry growth and its benefits to the soil environment was developed.The FAHP and CRITIC methods were used to calculate the subjective weight and objective weight of each index,respectively.The largest subjective weight was observed forY(0.200),and the largest objective weight was observed for soil-available potassium(0.101).The final weight was determined using Game theory;Y had the highest weight(0.185),and free amino acids(FAA)had the lowest weight(0.047).Grey relational analysis(GRA)was used to evaluate the optimal irrigation and potassium scheme for accomplishing multiple objectives.The response of the comprehensive score of strawberries to irrigation and potassium exhibited a negative parabolic relationship,and the effect of irrigation was greater than the effect of potassium application.There was a significant interaction between irrigation and potassium application,and an irrigation amount of 2053-2525 m^(3)/hm^(2) with a potassium application rate of 288.1-334.2 kg/hm^(2) was optimal for promoting strawberry yield,fruit quality,and efficiency and reducing soil nutrient residues.The results of this study provide new insights that could aid the development of sustainable approaches for enhancing agricultural production. | Xiaoqing Yang Rongcheng Du Mengchi Zhang Huawei Feng Ziqing Wang Zhi Zhang | 2023 | International Journal of Agricultural and Biological Engineering2023，16，2： | 0 |
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