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| 1 | An engineered xCas12i with high activity,high specificity,and broad PAM range显示文摘Dear Editor,The clustered regularly interspaced short palindromic repeats-Cas(CRISPR-Cas)systems,including type II Cas9 and type V Cas12 systems,which serve in the adaptive immunity of prokaryotes against viruses,have been developed into genome-editing tools(Anzalone et al.,2020;Doudna,2020).Compared with type II systems,the type V systems including V-A to V-K showed more functional diversity(Yan et al.,2019).Amongst them,Cas12i has a relatively smaller size(1,033-1,093 aa),compared to SpCas9 and Cas12a,and has a 5'-TTN protospacer adjacent motif(PAM)preference(Yan et al.,2019). | Hainan Zhang Xiangfeng Kong Mingxing Xue Jing Hu Zikang Wang Yinghui Wei Haoqiang Wang Jingxing Zhou Weihong Zhang Mengqiu Xu Xiaowen Shen Fengcai Yin Zhiyuan Ai Guangyan Huang Junhui Xia Xueqiong Song Hengbin Li Yuan Yuan Jinhui Li Na Zhong Meiling Zhang Yingsi Zhou Hui Yang | 2023 | Protein & Cell2023，14，7： | 0 |
| 2 | Back in time:digital restoration techniques for the millennium Dunhuang murals显示文摘In the long history of more than 1 500 years,Dunhuang murals suffered from various deteriorations causing irreversible damage such as falling off,fading,and so on.However,the existing Dunhuang mural restoration methods are time-consuming and not feasible to facilitate cultural dissemination and permanent inheritance.Inspired by cultural computing using artificial intelligence,gated-convolution-based dehaze net(GD-Net) was proposed for Dunhuang mural refurbishment and comprehensive protection.First,a neural network with gated convolution was applied to restore the falling off areas of the mural to ensure the integrity of the mural content.Second,a dehaze network was applied to enhance image quality to cope with the fading of the mural.Besides,a Dunhuang mural dataset was presented to meet the needs of deep learning approach,containing 1 180 images from the Cave 290 and Cave 112 of the Mogao Grottoes.The experimental results demonstrate the effectiveness and superiority of GD-Net. | Tao Yifeng Song Yucheng Xu Mengqiu Zhang Chuang Wu Ming Bai Sule | 2022 | The Journal of China Universities of Posts and Telecommunications2022，29，2： | 0 |
| 3 | A Recessive Mutant of argonaute 1b/gsnl4 Leads to Narrow Leaf, Small Grain Size and Low Seed Setting in Rice显示文摘A gsnl4 mutant characterized by small grain size,narrow leaf and low seed-setting rate was obtained by ethyl methane sulfonate(EMS)mutagenesis of a japonica rice variety Wuyunjing 21.Genetic analysis showed that gsnl4 is a loss-of-function mutant.A single-base mutation in gsnl4 resulted in the substitution of Ser to Asn in the Piwi domain of OsAGOlb protein.CRISPR/Cas9-mediated editing of OsAGOlb yielded a mutant phenotypically resembling gsnl4.Furthermore,miRNA-Seq analysis showed that the transcript expression levels of miRNAs in the signal transduction pathways related to pollen development,leaf morphology and honnone activation were significantly different between the gsnl4 mutant and the wild type(WT)plants.Several miRNAs were downregulated,and their target genes were upregulated in gsnl4 mutants.The auxin content in the root tips of the gsnl4 mutant decreased,and the expression of most auxin-related genes was altered.In summary,GSNL4 not only regulates organ development by controlling cell division and expansion,but also plays an important role in regulating auxin transport in rice. | SONG Mengqiu RUAN Shuang PENG Youlin WANG Zhongwei Jahan NOUSHIN ZHANG Yu CUI Yongtao HU Haitao JIANG Hongzhen DING Shilin SHEN Lan GAO Zhenyu HU Xingming QIAN Qian GUO Longbiao | 2021 | Rice science2021，28，6： | 0 |
| 4 | Simultaneous production of high-frequency synthetic apomixis with high fertility and improved agronomic traits in hybrid rice显示文摘Dear Editor,Utilization of heterosis is fundamental to modern agricultural improvement and contributes significantly to food security and agricultural development(Ouyang et al.,2022).However,heterosis of F,hybrids cannot be stably inherited.Apomixis,or asexual reproduction through seeds,offers the potential for the fixation of heterosis in F,hybrids and the formation of clonal offspring,which could have considerable implications for plant breeding and agriculture(Schmidt,2020).But naturally occurring apomixis has never been found in major crops.Recently,synthetic apomixis has been successfully generated by inducing incomplete meiotic development combined with parthenogenesis development to produce cloned rice(Oryza sativa)seeds(Khanday et al.,2019;Wang et al.,2019;Vernet et al.,2022;Wei et al.,2023). | Mengqiu Song Wumei Wang Chun Ji Shengnan Li Wei Liu Xiaoyu Hul Anhui Feng Shuang Ruan Shiyun Du Huan Wang Kui Dai Longbiao Guo Qian Qian Hongqi Si Xingming Hu | 2024 | Molecular Plant2024，17，1： | 0 |
| 5 | Cdc2-like kinases:structure,biological function,and therapeutic targets for diseases显示文摘The CLKs(Cdc2-like kinases)belong to the dual-specificity protein kinase family and play crucial roles in regulating transcript splicing via the phosphorylation of SR proteins(SRSF1–12),catalyzing spliceosome molecular machinery,and modulating the activities or expression of non-splicing proteins.The dysregulation of these processes is linked with various diseases,including neurodegenerative diseases,Duchenne muscular dystrophy,inflammatory diseases,viral replication,and cancer.Thus,CLKs have been considered as potential therapeutic targets,and significant efforts have been exerted to discover potent CLKs inhibitors.In particular,clinical trials aiming to assess the activities of the small molecules Lorecivivint on knee Osteoarthritis patients,and Cirtuvivint and Silmitasertib in different advanced tumors have been investigated for therapeutic usage.In this review,we comprehensively documented the structure and biological functions of CLKs in various human diseases and summarized the significance of related inhibitors in therapeutics.Our discussion highlights the most recent CLKs research,paving the way for the clinical treatment of various human diseases. | Mengqiu Song Luping Pang Mengmeng Zhang Yingzi Qu Kyle Vaughn Laster Zigang Dong | 2023 | Signal Transduction and Targeted Therapy2023，8，5： | 0 |
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