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| 1 | Combination of two multiplex genome-edited soybean varieties enables customization of protein functional properties显示文摘Dear Editor,Soybean(Glycine max)is a major source of plant-based protein for people worldwide,providing a healthy,affordable,and environmentally friendly alternative to animal-based protein(Montgomery,2003).It has been estimated that there are currently over 12000 food products that contain soy protein(Montgomery,2003).Different food applications of soy protein require distinct properties that affect its function and sensory features.For instance,the sensory quality of soymilk is significantly affected by protein emulsibility,whereas tofu quality is determined by protein-gelling ability(Kinsella,1979). | Mengyan Bai Cuicui Yuan Huaqin Kuang Qian Sun Xinchen Hu Luning Cui Wenxin Lin Chunyan Peng Peng Yue Shikui Song Zebin Guo Yuefeng Guan | 2022 | Molecular Plant2022，15，7： | 1 |
| 2 | Elimination of an unfavorable allele conferring pod shattering in an elite soybean cultivar by CRISPR/Cas9显示文摘Pod shattering can lead to devastating yield loss of soybean and has been a negatively selected trait in soybean domestication and breeding.Nevertheless,a significant portion of soybean cultivars are still pod shattering-susceptible,limiting their regional and climatic adaptabilities.Here we performed genetic diagnosis on the shattering-susceptible trait of a national registered cultivar,Huachun6(HC6),and found that HC6 carries the susceptible genotype of a candidate Pod dehiscence 1(PDH1)gene,which exists in a significant portion of soybean cultivars.We next performed genome editing on PDH1 gene by clustered regularly interspaced short palindromic repeats(CRISPR)-CRISPR-associated protein 9(Cas9).In T2 progenies,several transgene-free lines with pdh1 mutations were characterized without affecting major agronomic traits.The pdh1 mutation significantly improved the pod shattering resistance which is associated with aberrant lignin distribution in inner sclerenchyma.Our work demonstrated that precision breeding by genome editing on PDH1 holds great potential for precisely improving pod shattering resistance and adaptability of soybean cultivars. | Zhihui Zhang Jie Wang Huaqin Kuang Zhihong Hou Pingping Gong Mengyan Bai Shaodong Zhou Xiaolei Yao Shikui Song Long Yan Yuefeng Guan | 2022 | aBIOTECH2022，3，2： | 0 |
| 3 | Multiplex genome editing targeting soybean with ultra-low anti-nutritive oligosaccharides显示文摘Soybean is the primary source of plant protein for humans.Owing to the indigestibility of the raffinose family of oligosaccharides(RFO),raffinose and stachyose are considered anti-nutritive factors in soybean seeds.Low-RFO soybean cultivars are generated by mutagenesis of RFO biosynthesis genes,but the carbohydrate profiles invite further modification to lower RFOs.This study employed a pooled multiplex genome editing approach to target four seed-specifically expressed genes mediating RFO biosynthesis,encoding three raffinose synthases(RS2,RS3,and RS4)and one stachyose synthase.In T1progeny,rs2/rs3 and rs4/sts homozygous double mutants and a rs2/rs3/rs4/sts quadruple mutant(rfo-4m)were characterized.The rs2/rs3 mutant showed reduced raffinose and stachyose contents,but the rs4/sts mutant showed only reduced stachyose in seeds.The RFO contents in the rfo-4m mutant were almost eliminated.Metabolomic analysis showed that the mutation of four RFO biosynthesis genes led to a shift of metabolic profile in the seeds,including the accumulation of several oligosaccharides-related metabolites.These mutants could contribute to precision breeding of soybean cultivars for soy food production. | Wenxin Lin Huaqin Kuang Mengyan Bai Xiaomeng Jiang Pengfei Zhou Yinghua Li Bo Chen Huarong Li Yuefeng Guan | 2023 | The Crop Journal2023，11，3： | 0 |
| 4 | Expressing a human RNA demethylase as an assister improves gene-editing efficiency in显示文摘Dear Editor,Despite myriad successful applications of gene editing in plant functional genomics research and precision breeding,many challenges persist around the efficiency of gene-editing tools for many plant species.For instance,soybean(Glycine max)is a major crop providing oil and protein to human diets and feedstock,but its gene-editing efficiency remains relatively low(Bai et al.,2019). | Mengyan Bai Wenxin Lin Chunyan Peng Peizhe Song Huaqin Kuang Jieni Lin Jieping Zhang Jiyao Wang Bo Chen Huarong Li Fanjiang Kong Guifang Jia Yuefeng Guan | 2024 | Molecular Plant2024，17，3： | 0 |
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