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| 1 | Exosome and Exosomal MicroRNA: Trafficking, Sorting, and Function显示文摘Exosomes are 40–100 nm nano-sized vesicles that are released from many cell types into the extracellular space. Such vesicles are widely distributed in various body fluids. Recently,m RNAs and micro RNAs(mi RNAs) have been identified in exosomes, which can be taken up by neighboring or distant cells and subsequently modulate recipient cells. This suggests an active sorting mechanism of exosomal mi RNAs, since the mi RNA profiles of exosomes may differ from those of the parent cells. Exosomal mi RNAs play an important role in disease progression, and can stimulate angiogenesis and facilitate metastasis in cancers. In this review, we will introduce the origin and the trafficking of exosomes between cells, display current research on the sorting mechanism of exosomal mi RNAs, and briefly describe how exosomes and their mi RNAs function in recipient cells.Finally, we will discuss the potential applications of these mi RNA-containing vesicles in clinical settings. | Jian Zhang Sha Li Lu Li Meng Li Chongye Guo Jun Yao Shuangli Mi | 2015 | Genomics, Proteomics & Bioinformatics2015，13，1： | 162 |
| 2 | Prevalence of minimal hepatic encephalopathy and quality of life evaluations in hospitalized cirrhotic patients in China显示文摘AIM:To investigate the prevalence of minimal hepatic encephalopathy(MHE)and to assess corresponding health-related quality of life(HRQoL)in hospitalized cirrhotic patients in China.METHODS:This multi-center cross-sectional study included 16 teaching hospitals,which were members of 'Hepatobiliary Cooperation Group,Society of Gastroenterology,Chinese Medical Association',from different areas of China carried out between June and October in 2011.All the eligible hospitalized cirrhotic patients(n = 538)were required to complete triplicate number connection tests combined with one digit symbol test for diagnosing MHE.Patients' clinical examination data were complemented by a modified questionnaire assessing HRQoL.Written informed consent was obtained from each patient.RESULTS:Male was predominant(68.6%)in 519 patients who met the criteria of the study,with a mean age of 49.17 ± 11.02 years.The most common cause of liver cirrhosis was chronic hepatitis B(55.9%).The prevalence of MHE was 39.9% and varied by ChildPugh-Classification score(CPC-A:24.8%,CPC-B:39.4% and CPC-C:56.1%,P < 0.01).MHE(P < 0.01)and higher CPC scores(P < 0.01)were associated with a high HRQoL scores(reflecting poorer quality of life).The prevalence of MHE was proportionate to CPC(P = 0.01)and high quality of life scores(P = 0.01).CONCLUSION:Hospitalized cirrhotic patients have a high prevalence of MHE that is proportionate to the degree of liver function and HRQoL impairment. | Ji-Yao Wang Ning-Ping Zhang Bao-Rong Chi Yu-Qing Mi Li-Na Meng Ying-Di Liu Jiang-Bin Wang Hai-Xing Jiang Jin-Hui Yang Yun Xu Xiao Li Jian-Ming Xu Guo Zhang Xin-Min Zhou Yu-Zheng Zhuge De-An Tian Jin Ye Yu-Lan Liu | 2013 | World Journal of Gastroenterology2013，19，30： | 64 |
| 3 | High-Strength and High-Plasticity TWIP Steel for Modern Vehicle显示文摘In this paper new high-strength and high-plasticity twinning induced plasticity (TWIP) steel for modern automobile body was investigated. Some basic experimental results were given. The results indicate the TWlP steel has excellent properties. It exhibits high ultimate tensile strength (600~1100 MPa) and extremely large elongation of 60% to 90%. In the future it would be capable of satisfying the requirements of new generation of vehicle. | Zhenli MI Di TANG Ling YAN Jin GUO | 2005 | Journal of Materials Science & Technology2005，21，4： | 25 |
| 4 | Prevalence of Thyroid Nodules and Its Relationship with Iodine Status in Shanghai:a Population-based Study显示文摘Objective This study was designed to evaluate the prevalence of thyroid nodules(TNs) and its relationship with urine iodine concentrations(UICs) after the regional rapid economic growth and lifestyle changes. Methods A cross-sectional survey was conducted in the general population aged 15-69 years. A questionnaire regarding general and personal characteristics and relevant information was administered. Ultrasonography of the thyroid was performed, and serum triiodothyronine(T3), tetraiodothyronine(T4), serum thyroid stimulating hormone(TSH), free triiodothyronine(FT3), free tetraiodothyronine(FT4), thyroglobulin antibody(Tg Ab), thyroid peroxidase antibody(TPOAb), and TSH receptor antibody(TRAb) levels were measured for each individual subject. Results The prevalence rates of TNs in the whole population, females and males were 27.76%, 34.04%, and 21.60%, respectively. The prevalence of multiple nodules increased with age, whereas the prevalence peaks differed between males and females. The median UICs in the whole population and females with non-TNs were higher than those of subjects with TNs(P=0.0035, P=0.0068). The median UICs in subjects with a single TN were higher than those in subjects with multiple TNs(P=0.0164, P=0.0127). The result showed a U-shaped curve relationship between UIC and prevalence of TNs. The prevalence of TNs was the lowest when the UIC was 140-400 μg/L. Conclusion The prevalence of TNs was nearly 30% and increased with age. The relationship between UIC and prevalence of TNs is U-shaped, with an increase in risk when the UIC was <140 μg/L and >400 μg/L. Very low or high UIC levels need attention and correction. | SONG Jun ZOU Shu Rong GUO Chang Yi ZANG Jia Jie ZHU Zhen Ni MI Ming HUANG Cui Hua YU Hui Ting LU Xi RUAN Ye WU Fan | 2016 | Biomedical and Environmental Sciences2016，29，6： | 25 |
| 5 | Crystal structure and biochemical analyses reveal Beclin 1 as a novel membrane binding protein显示文摘Beclin 1 基因是 haplo 不够的肿瘤 suppressor 并且在 autophagy 起一个必要作用。然而, Beclin 1 由工作的分子的机制仍然保持大部分未知。这里,我们在 1.6 Å 报导 Beclin 1 的 evolutionarily 保存的领域(ECD ) 的水晶结构;分辨率。Beclin 1 ECD 展览一以前 unreported 褶层,与在中央轴附近相称性地安排的三次结构的重复。Beclin 1 ECD 定义膜绑定领域的一个新奇的类,与为与 cardiolipin 充实的类脂化合物膜的强壮的偏爱。在 Beclin 1 ECD 的一个表面环的尖端,包括三芳香的氨基酸,与类脂化合物膜充当一根恐水病的手指到伙伴,因而导致膜和 liposomes 的变丑。这些芳香的残余的变化使 Beclin 1 变为不能稳定地在 vitro 与类脂化合物膜联系并且不能充分在 vivo 在 Beclin 1-knockdown 房间救 autophagy。这些观察为译解形成一个重要框架 Beclin 1 的生物功能。 | Weljlao Huang Wooyoung Chol Wanqiu Hu Na Mi Qiang Guo Meisheng Ma Mei Liu Yuan Tian Peilong Lu Feng-Liang Wang Haiteng Deng Lei Liu Ning Gao Li Yu Yigong Shi | 2012 | Cell Research2012，22，3： | 21 |
| 6 | Antigenic variation of the human influenza A(H3N2) virus during the 2014–2015 winter season显示文摘The human influenza A(H3N2) virus dominated the 2014–2015 winter season in many countries and caused massive morbidity and mortality because of its antigenic variation. So far, very little is known about the antigenic patterns of the recent H3N2 virus. By systematically mapping the antigenic relationships of H3N2 strains isolat ed since 2010, we discov ered that two groups with obvious antigenic divergence, named SW13(A/Switzerland/9715293/2013-like strains) and HK14(A/Hong Kong/5738/2014-like strains), co-circulated during the 2014–2015 winter season. HK14 group co-circulated with SW13 in Europe and the United States during this season, while there were few strains of HK14 in China's Mainland, where SW13 has dominated since 2012. Furthermore, we found that substitutions near the receptor-binding site on hemagglutinin played an important role in the antigenic variation of both the groups. These findings provide a comprehensive understanding of the recent antigenic evolution of H3N2 virus and will aid in the selection of vaccine strains. | HUA Sha LI XiYan LIU Mi CHENG YanHui PENG YouSong HUANG WeiJuan TAN MinJu WEI HeJiang GUO JunFeng WANG DaYan WU AiPing SHU YueLong JIANG TaiJiao | 2015 | Science China(Life Sciences)2015，58，9： | 17 |
| 7 | An analysis of patients receiving emergency CAG without PCI and the value of GRACE score in predicting PCI possibilities in NSTE-ACS patients显示文摘BackgroundThere 是经历了紧急情况的病人冠的 angiography (CAG ) 但是没收到经皮的冠的干预(一种总线标准) 。学习是分析这些 reasons.MethodsThis 的这的目的是单个中心的回顾的研究。我们招募了 201 consecutive 收到了紧急情况 CAG 的病人但是没收到一种总线标准。为了在在非圣预言一种总线标准可能性调查尖锐冠的事件(优雅)分数的全球注册表的值,分割举起急性冠的症候群(NSTE交流)病人,我们招募了 80 ;与NSTE交流介绍了并且收到了紧急情况 CAG 以及紧急情况 PCI.ResultsAmong 的连续病人收到了紧急情况 CAG 的 201 个病人但是没收到一种总线标准,26%病人有除冠的心疾病以外的最后的诊断。在有重要冠的动脉狭窄的病人, 23 个病人(11.5%) 被推荐到冠的动脉绕过 grafting (CABG ) ,一个病人(0.5%) 拒绝了一种总线标准;有重要血栓负担的 13 个病人(6.5%) 与 glycoprotein IIb/IIIa 受体对手被对待;74 个病人(36.8%) 与药治疗被对待因为没有严重狭窄(>70%) 在罪容器是在场的。而且, 201 个病人中的 80 个与 NSTE 交流被介绍(与除冠的心疾病以外的最后的诊断排除那些病人,排除那些病人为 CABG 治疗计划了) ,是参考了非一种总线标准 NSTE 交流。当比较时,他们的优雅得分,象紧急情况一种总线标准一样收到了紧急情况 CAG 的 80 个连续病人与 NSTE 交流介绍了(作为一种总线标准 NSTE 交流参考了) ,我们发现一种总线标准 NSTE 交流病人有显著地更高的优雅分数与相比非一种总线标准 NSTE 交流病人。我们然后使用了接收装置操作员典型曲线(巨鸟) 测试 GRACE 分数是否擅长在 NSTE 交流病人评估一种总线标准的可能性。在 curve 下面的区域;是 0.854 ±0.030 (P <0.001 ) ,显示好预兆的价值。而且,我们分析了从巨鸟统计导出的结果,并且发现那 125.5 的一个优雅分数,作为一截止,在在 NSTE 交流 patients.ConclusionsOur 调查结果评估一种总线标准可能性让高敏感和特性显示 GRACE 分数在决定 NSTE 交流病人是否将收到一种总线标准有预兆的价值。 | Bo-Da ZHOU | 2015 | Journal of Geriatric Cardiology2015，12，3： | 15 |
| 8 | The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS Rats显示文摘Objective To explore the Intervention effect of Rosiglitozone in ovarian fibrosis of PCOS rats.Methods 60 female SD rats were randomly divided into 3 groups:control group,model group and treatment group.The model and treatment groups were established by subcutaneous injection of DHEA,while the treatment group was given RGZ.The serum hormone values,pathohistology of ovarian structure of rats,ovarian ultrastructure and the expressions of TGF-β 1 and CTGF were detected.Results The PCOS model was established successfully.The expression intensity of TGF-β 1 and CTGF in Oocytes of the PCOS groups was 9.545±2.954 and 9.665±2.400,respectively and was significantly higher than that of the control group 6.636±2.264 and 7.036±2.133;after treatment with rosiglitazone,the expression was significantly decreased 6.980±2.421 and 6.642±2.721 as compared with that of the model group (P<0.05,P<0.001).The values in serum of the PCOS groups were 3.749±2.054 and 0.265±0.129,and 1.914±1.801 and 0.096±0.088 in the control group which had statistically significant difference (P<0.05,P<0.001).After treatment with rosiglitazone,the values were 2.3100±1.825 and 0.112±0.187 and were significantly different with those of the model group (P<0.05,P<0.001).Conclusion TGF-β 1 and CTGF play an important role in the development of ovary fibrosis in PCOS.However,RGZ may postpone the development of fibrosis by decreasing the levels of TGF-β 1 and CTGF. | MIAO Zhu Lin GUO Liang WANG Yong Xia CUI Rong YANG Ning HUANG Mi Qiong QIN Wei Bing CHEN Jin LI Hong Mei WANG Zi Neng WEI Xiang Cai | 2012 | Biomedical and Environmental Sciences2012，25，1： | 14 |
| 9 | Uptake of HIV Self-testing among Men Who have Sex with Men in Beijing, China: a Cross-sectional Study显示文摘Objective To examine HIV self-testing uptake and its determinates among men who have sex with men(MSM) in Beijing, China. Methods A cross-sectional online survey was conducted in Beijing, China in 2016. Participants were users of a popular Chinese gay networking application and had an unknown or negative HIV status. Univariate and multivariate logistic regression analyses were conducted to examine factors associated with HIV self-testing based on adjusted odds ratio(AOR) and 95% confidence interval(CI). Results Among the 5,996 MSM included in the study, 2,383(39.7%) reported to have used HIV self-testing kits. Willingness to use an HIV self-test kit in the future was expressed by 92% of the participants. High monthly income(AOR = 1.49; CI = 1.10-2.02; P = 0.010), large number of male sex partners(≥ 2: AOR = 1.24; CI = 1.09-1.43; P = 0.002), sexual activity with commercial male sex partners(≥ 2: AOR = 1.94; CI = 1.34-2.82; P = 0.001), long-term drug use(AOR = 1.42; CI = 1.23-1.62; P < 0.001), and long-term HIV voluntary counseling and testing(VCT) attendance(AOR = 3.62; CI = 3.11-4.22; P < 0.001) were all associated with increased odds of HIV self-testing uptake. Conclusion The nearly 40% rate of HIV self-testing uptake among MSM in our sample was high. In addition, an over 90% willingness to use kits in the future was encouraging. HIV self-testing could be an important solution to help China achieve the global target of having 90% of all people living with HIV diagnosed by 2020. | REN Xian Long WU Zun You MI Guo Dong MCGOOGAN Jennifer ROU Ke Ming ZHAO Yan | 2017 | Biomedical and Environmental Sciences2017，30，6： | 13 |
| 10 | Bioactive biodegradable polycitrate nanoclusters enhances the myoblast differentiation and in vivo skeletal muscle regeneration via p38 MAPK signaling pathway显示文摘Complete skeletal muscle repair and regeneration due to severe large injury or disease is still a challenge.Biochemical cues are critical to control myoblast cell function and can be utilized to develop smart biomaterials for skeletal muscle engineering.Citric acid-based biodegradable polymers have received much attention on tissue engineering,however,their regulation on myoblast cell differentiation and mechanism was few investigated.Here,we find that citrate-based polycitrate-polyethylene glycol-polyethylenimine(POCG-PEI600)nanoclusters can significantly enhance the in vitro myoblast proliferation by probably reinforcing the mitochondrial number,promote the myotube formation and full-thickness skeletal muscle regeneration in vivo by activating the myogenic biomarker genes expression of Myod and Mhc.POCG-PEI600 nanoclusters could also promote the phosphorylation of p38 in MAP kinases(MAPK)signaling pathway,which led to the promotion of the myoblast differentiation.The in vivo skeletal muscle loss rat model also confirmed that POCG-PEI600 nanoclusters could significantly improve the angiogenesis,myofibers formation and complete skeletal muscle regeneration.POCG-PEI600 nanocluster could be also biodegraded into small molecules and eliminated in vivo,suggesting their high biocompatibility and biosafety.This study could provide a bioactive biomaterial-based strategy to repair and regenerate skeletal muscle tissue. | Yi Guo Min Wang Juan Ge Wen Niu Mi Chen Wei Cheng Bo Lei | 2020 | Bioactive Materials2020，5，3： | 12 |
| 11 | RNA Interference-mediated Silencing of Phytochelatin Synthase Gene Reduce Cadmium Accumulation in Rice Seeds显示文摘Phytochelatins (PC ) 玩在重金属抵抗和累积的一个重要角色。到在大米的镉(Cd ) 的累积播种的还原剂,表示 ofphytochelatin synthase (PCS ) 基因 OsPCSI 被 RNAinterference (RNAi ) 压制。PCS 碎片的发卡构造在控制 ofZMM1 下面在 pRNAi-OsPCS1 被设计,从玉米的 aseed 特定的倡导者。构造被介绍进米饭(装饰用的梨树) throughAgrobacteriumtumefaciens。RNAi 米饭小植物在壶 exposured to10 mg/kg Cd 被选择并且栽培。OsPCSI 的 transcriptionallevel 与野生型相比在一些 RNAi 米饭的种子衰退了。作为结果 Cd 累积被减少由关于在 RNAi 大米的种子的一半。Asexpected,生长的明显的差别都没出现在 RNAiand 野类型的工厂之间。结果建议这条新途径能被用来在庄稼控制重金属累积。 | Jiang-Chuan Li Jiang-Bo Guo Wen-Zhong Xu Mi Ma | 2007 | Journal of Integrative Plant Biology2007，49，7： | 11 |
| 12 | Effects of recombinant retroviral vector mediated human insulin like growth factor-1 gene transfection on skeletal muscle growth in rat显示文摘Background This study transferred a recombinant gene encoding human insulin like growth factor-1 (hIGF-1) into modified primary skeletal myoblasts with a retroviral vector (pLgXSN ) and determined whether the hIGF-1 promoted growth of skeletal muscle in rat. Methods hIGF-1cDNA was amplified in vitro from normal human liver cells by using RT-PCR and cloned into plasmid vector pLgXSN. The recombinant vector pLghIGF-1SN and control vector pLgGFPSN were transfected into packaging cell PT67 and G418 was used to select positive colony. Myoblasts were infected with a high titre viral supernatant and transduction efficiency was evaluated as GFP expression. The expression of hIGF-1 mRNA in myoblasts was investigated by immunocytochemistry and RT-PCR. MTT assays detected the growth of myoblasts in vitro. Myoblasts transduced with pLghIGF-1SN were injected into hind limb muscles of 10-12 week male SD rats. Formed tissues were harvested 4 weeks later. Myocyte diameter, mean weight of hind limb and body were measured to evaluate the skeletal muscle growth. Results Recombinant retroviral plasmid vector pLghIGF-1SN was constructed successfully. The titre of the packaged recombinant retrovirus was 1×106 cfu/ml. The transfection rate of PT67 cells reached 100% after G418 screening. hIGF-1 expression was positive in myoblast-IGF-1. The proliferation rate of myoblast-IGF-1 in vitro was higher than GFP-myoblast or myoblast (P< 0.05). The mean weights of hind limb and body of rats injected myoblast-IGF-1 were higher than those of the rats injected with myoblast-GFP or myoblast (P< 0.05). Myocyte diameter had a significant increase in IGF-1 group compared to GFP group and myoblast group (P< 0.05). Conclusions The transfection of the human IGF-1 gene mediated by a retroviral vector can promote the growth of skeletal muscle in rats. Genetically modified primary skeletal myoblasts provide a possibly effective approach to treat some skeletal muscle diseases. | RONG Shu-Ling LU Yong-Xin LIAO Yu-Hua WANG Xiao-Lin GUO He-Ping CHANG Chao GAO Yan-Zhang MI Shao-Hua Wan Jian-Ping | 2006 | Chinese Medical Journal2006，，23： | 10 |
| 13 | Microstructure and tensile properties of low cost titanium alloys at different cooling rate显示文摘Titanium and titanium alloys have several advantages, but the cost of titanium alloys is very expensive compared with the traditional metal materials. This article introduces two new low-cost titanium alloys Ti-2.1Cr-1.3Fe (TCF alloy) and Ti-3Al-2.1Cr-1.3Fe (TACF alloy). In this study, we used Cr-Fe master alloy as one of the raw materials to develop the two new alloys. We introduce the microstructure and tensile properties of the two new alloys from β solution treated with different cooling methods. Optical microscopy (OM), X-ray diffractometry (XRD), and transmission electron microscopy (TEM) were employed to analyze the phase constitution, and scanning electron microscopy (SEM) was used to observe the fracture surfaces. The results indicate that the microstructures consist of β grain boundary and α′ martensite after water quenching (WQ), β matrix and α phase after air cooling (AC) and furnace cooling (FC), respectively. Also, the microstructure is the typical basketweave structures after FC. Of course, athermal ω is also observed by TEM after WQ. The strength increases with decreasing cooling rates and the plasticity is reversed. Because of the athermal ω, the strength and ductility are highest and lowest when the cooling method is WQ. The strength of TACF alloy is higher than the TCF alloy, but the plasticity is lower. The fracture surfaces are almost entirely covered with dimples under the cooling methods of AC and FC. Also, we observe an intergranular fracture area that is generated by athermal ω, although some dimples are observed after WQ. | Wang Guo Hui Songxiao Ye Wenjun Mi Xujun Wang Yongling Zhang Wenjing | 2012 | Rare Metals2012，31，6： | 8 |
| 14 | Enhanced Cadmium Accumulation in Transgenic Tobacco Expressing the Phytochelatin Synthase Gene of Cynodon dactylon L显示文摘Bermudagrass (L 上的 Cynodon 扬抑抑格。cv。Goldensun ) 对高度抵抗并且积累大量镉(Cd ) 。phytochelatin synthase (PCS ) cDNA (CdPCSI ) 被 cDNA 结束的快速的扩大从这棵草孤立。通常认为的 CdPCSI 蛋白质从另外的植物与 PCS 分享了高相同,与在 N 终端的 79% 相同和在 theC 终端的 47% 相同。然而, 16 Cys 残余在 CdPCS1 的 C 终端被发现,并且在这些残余之中,三个位置与另外的 PCS 蛋白质不同。Semiquantitative reversetranscription 聚合酶链反应分析证明 Cd 应力在 Bermudagrass 在根和叶子导致了 CdPCSI 表示。我们证实 CdPCSI 由在 ABDE1 表示基因在酵母房间在 Cdtolerance 起一个重要作用, Cd 敏感的异种。CdPCSI 然后被介绍进烟草植物。在某转基因的烟草的 phytochelatin 水平在野生型植物非常衬里 increased3.88 褶层,在这些转基因的植物的 Cd 累积因此是 enhanced3.21 褶层。结果建议 CdPCSI 以后能为植物救治被用作一个基因要素。 | Jiangchuan Li Jiangbo Guo Wenzhong Xu Mi Ma | 2006 | Journal of Integrative Plant Biology2006，48，8： | 8 |
| 15 | Influence of Inclusion on Crack Initiation in Wheel Rim显示文摘Speed increase and heavy haul of the railway freight car are the effective measures to raise the transport ability of railway,and they have been the new trend for railway freight car development.The increase of speed and axle load will lead to the increase of wheel-rail contact stress,which will make a great change of stress distribution in the whole wheel rim.Goodier equation was employed to analyze the stress state around inclusion and cavity.In the operation of wheel-rail contact stress,stress concentration occurred in the pole of Al2O3 spherical inclusion,while it appeared on the equator of cavity.The critical inclusion sizes of 250 kN axle load freight car wheel at different velocities,a certain distance away from the tread,were calculated by Murakami equation.Comparing the inclusion in cast steel wheel with that in rolled steel wheel,it is found that the inclusion in cast steel wheel has much more excellent crack resistance than that in rolled steel wheel.In the meantime,in order to reduce the probability of rim crack,some suggestions were put forward. | MI Guo fa NAN Hong yan LIU Yan-lei ZHANG Bin ZHANG Hong SONG Guo xiang | 2011 | Journal of Iron and Steel Research(International)2011，18，1： | 8 |
| 16 | Efficacy of a Chinese herbal formula on hepatitis B e antigenpositive chronic hepatitis B patients显示文摘BACKGROUND No guideline recommends antiviral therapy for hepatitis B e antigen(HBeAg)-positive chronic hepatitis B patients with persistently normal alanine aminotransferase levels and a high hepatitis B virus(HBV)DNA viral load.AIM To evaluate the feasibility and safety of a Chinese herbal formula as a therapeutic option for chronic HBV infection.METHODS In total,395 patients(30–65 years old)with confirmed HBeAg-positive chronic hepatitis B infection and persistently normal alanine aminotransferase were randomized to receive either Chinese herbal formula or placebo for 96 wk.Endpoints to evaluate therapeutic efficacy included:(1)HBV DNA levels decreased to less than 4 log10 IU/mL at weeks 48 and 96;and(2)HBeAg clearance and seroconversion rates at weeks 48 and 96.RESULTS HBV DNA levels≤4 log10 IU/mL were 10.05%at week 48 and 18.59%at week 96 in the treatment group.The HBeAg clearance and conversion rates were 8.54%and 8.04%at week 48 and 16.08%and 14.57%at week 96,respectively.However,HBV DNA levels≤4 log10 IU/mL were 2.55%and 2.55%at weeks 48 and 96,respectively,and the HBeAg clearance rates were 3.06%and 5.61%at weeks 48 and 96,respectively,in the control group.The quantitative hepatitis B surface antigen and HBeAg levels at baseline and changes during the treatment period as well as the alanine aminotransferase elevation at weeks 12 and 24 were strong predictors of HBeAg clearance.CONCLUSION High rates of HBV DNA reduction,HBeAg clearance and seroconversion could be achieved with Chinese herbal formula treatments,and the treatments were relatively safe for HBeAg-positive chronic hepatitis B-infected patients with persistently normal alanine aminotransferase.The ability of the compound to modulate host immune function probably contributed to this effect. | Yu-Feng Xing Chun-Shan Wei Tian-Ran Zhou Dan-Ping Huang Wei-Chao Zhong Bin Chen Hua Jin Xiao-Yu Hu Zhi-Yun Yang Qing He Kai-Ping Jiang Jun-Min Jiang Zhen-Bin Hu Xin Deng Fan Yang Feng-Yi Li Gang Zhao Li-Chun Wang Yu-Qiang Mi Zuo-Jiong Gong Peng Guo Jian-Hua Wu Wei-Qun Shi Hong-Zhi Yang Da-Qiao Zhou Guang-Dong Tong | 2020 | World Journal of Gastroenterology2020，26，30： | 7 |
| 17 | An-Luo-Hua-Xian Pill Improves the Regression of Liver Fibrosis in Chronic Hepatitis B Patients Treated with Entecavir显示文摘Background and Aims:Chronic hepatitis B(CHB)can cause liver fibrosis and lead to cirrhosis and cancer.As the effectiveness of antiviral therapy to reverse liver fibrosis is limited,We aimed to evaluate the effect of An-Luo-Hua-Xian pill(ALHX)on fibrosis regression in CHB patients treated with entecavir(ETV).Methods:Treatment-naïve patients with CHB were randomly treated with ETV alone or combined with ALHX(ETV+ALHX)between October 1,2013 and December 31,2020.Demographic,laboratory,and liver histology data before and after 78 weeks of treatment were collected.The Ishak fibrosis score(F)was used and fibrosis regression required a decrease in F of≥1 after treatment.Results:A total of 780 patients were enrolled,and 394 with a second liver biopsy after treatment were included in the per-protocol population,132 in ETV group and 262 in ETV+ALHX group.After 78 weeks of treatment,the fibrosis regression rate in the ETV+ALHX group was significantly higher than that of the ETV group at baseline F≥3 patients:124/211(58.8%)vs.45/98(45.9%),p=0.035.The percentage of patients with a decreased liver stiffness measurement(LSM)was higher in the ETV+ALHX group:156/211(73.9%)vs.62/98(63.%),p=0.056.Logistic regression analysis showed that ETV combined with ALHX was associated with fibrosis regression[odds ratio(OR)=1.94,p=0.018],and a family history of hepatocellular carcinoma was on the contrary.(OR=0.41,p=0.031).Conclusions:ETV combined with ALHX increased liver fibrosis regression in CHB patients. | Yi-Qi Liu Chi Zhang Jia-Wen Li Li-Hua Cao Zhan-Qing Zhang Wei-Feng Zhao Qing-Hua Shang Da-Zhi Zhang An-Lin Ma Qing Xie Hong-Lian Gui Guo Zhang Ying-Xia Liu Jia Shang Shi-Bin Xie Jun Li Xu-Qing Zhang Zhi-Qiang Zou Yu-Ping Chen Zong Zhang Ming-Xiang Zhang Jun Cheng Fu-Chun Zhang Li-Hua Huang Jia-Bin Li Qing-Hua Meng Hai-Bin Yu Yu-Qiang Mi Yan-Zhong Peng Zhi-Jin Wang Li-Ming Chen Fan-Ping Meng Wan-Hua Ren Lang Bai Yi-Lan Zeng Rong Fan Xian-Zhi Lou Wei-Feng Liang Hui Liu Hui Zhuang Hong Zhao Gui-Qiang Wang | 2023 | Journal of Clinical and Translational Hepatology2023，11，2： | 7 |
| 18 | Protective effect of total flavonoid C-glycosides from Abrus mollis extract on lipopolysaccharide-induced lipotoxicity in mice显示文摘Abrus mollis is a widely used traditional Chinese medicine for treating acute and chronic hepatitis, steatosis, and fibrosis. It was found that the total flavonoid C-glycosides from Abrus mollis extract(AME) showed potent antioxidant, anti-inflammatory, and hepatoprotective activities. To further investigate the hepatoprotective effect of AME and its possible mechanisms, lipopolysaccharide(LPS)-induced liver injury models were applied in the current study. The results indicated that AME significantly attenuated LPS-induced lipid accumulation in mouse primary hepatocytes as measured by triglyceride(TG) and total cholesterol(TC) assays and Oil Red O staining. Meanwhile, AME exerted a protective effect on LPS-induced liver injury as shown by decreased liver index, serum aminotransferase levels, and hepatic lipid accumulation. Real-time PCR and immunoblot data suggested that AME reversed the LPS-mediated lipid metabolism gene expression, such as sterol regulatory element-binding protein-1(SREBP-1), fatty acid synthase(FAS), and acetyl-CoA carboxylase 1(ACC1). In addition, LPS-induced overexpression of activating transcription factor 4(ATF4), X-box-binding protein-1(XBP-1), and C/EBP homologous protein(CHOP) were dramatically reversed by AME. Furthermore, AME also decreased the expression of LPS-enhanced interleukin-6(IL-6) and cyclooxygenase-2(COX-2). Here, it is demonstrated for the first time that AME ameliorated LPS-induced hepatic lipid accumulation and that this effect of AME can be attributed to its modulation of hepatic de novo fatty acid synthesis. This study also suggested that the hepatoprotective effect of AME may be related to its down-regulation of unfolded protein response(UPR) activation. | WANG Yun JIANG Zhen-Zhou CHEN Mi WU Mei-Juan GUO Hong-Li SUN Li-Xin WANG Hao ZHANG Shuang WANG Tao ZHANG Lu-Yong | 2014 | Chinese Journal of Natural Medicines2014，12，6： | 7 |
| 19 | Agglutinin isolated from Arisema heterophyllum Blume induces apoptosis and autophagy in A549 cells through inhibiting PI3K/Akt pathway and inducing ER stress显示文摘Arisaema heterophyllum Blume is one of the three medicinal plants known as traditional Chinese medicine Rhizoma Arisaematis(RA). RA has been popularly used to treat patients with convulsions, inflammation, and cancer for a long time. However, the underlying mechanisms for RA effects are still unclear. The present study was designed to determine the cytotoxicity of agglutinin isolated from Arisema heterophyllum Blume(AHA) and explore the possible mechanisms in human non-small-cell lung cancer A549 cells. AHA with purity up to 95% was isolated and purified from Arisaema heterophyllum Blume using hydrophobic interaction chromatography. AHA dose-dependently inhibited the proliferation of A549 cells and induced G_1 phase cell cycle arrest. AHA induced apoptosis by up-regulating pro-apoptotic Bax, decreasing anti-apoptotic Bcl-2, and activating caspase-9 and caspase-3. In A549 cells treated with AHA, the PI3K/Akt pathway was inhibited. Furthermore, AHA induced increase in the levels of ER stress markers such as phosphorylated eukaryotic initiation factor 2α(p-eIF2α), C/EBP-homologous protein(CHOP), inositol-requiring enzyme 1α(IRE1α), and phosphorylated c-Jun NH_2-terminal kinase(p-JNK). AHA also induced autophagy in A549 cells. Staining of acidic vesicular organelles(AVOs) and increase in the levels of LC3II and ATG7 were observed in AHA-treated cells. These findings suggested that AHA might be one of the active components with anti-cancer effects in Arisaema heterophyllum Blume. In conclusion, cytotoxicity of AHA on cancer cells might be related to its effects on apoptosis and autophagy through inhibition of PI3K/Akt pathway and induction of ER stress. | FENG Li-Xing SUN Peng MI Tian LIU Miao LIU Wang YAO Si CAO Yi-Min YU Xiao-Lu WU Wan-Ying JIANG Bao-Hong YANG Min GUO De-An LIU Xuan | 2016 | Chinese Journal of Natural Medicines2016，14，11： | 6 |
| 20 | Stable carbon and nitrogen isotope evidence of human and pig diets at the Qinglongquan site,China显示文摘Previous studies on the Rice-Millet(foxtail millet and common millet) Blended Zone in Chinese Neolithic have not clearly addressed such questions as the importance of primitive rice-millet mixed agriculture to human lifestyle and livestock managements within this region,the relationship among the development of the agriculture,paleoenvironment,and cultural interactions,and so on.Here stable carbon and nitrogen isotope analysis of human and pig bones from the Qinglongquan site was conducted,covering two cultural phases,namely the Qujialing Culture(3000 BC to 2600 BC) and the Shijiahe Culture(2600 BC to 2200 BC) .Based on this analysis,we further discussed the diets of ancient humans and pigs in the site,investigated the importance of rice-millet mixed agriculture to human and pig diets,and explored the relationship among the primitive rice agriculture and millet agriculture,cultural interactions,and paleoenvironment.The δ13C values of human bone collagen(-16.7‰ to-12.4‰,averaging-14.6‰±1.3‰,n=24) revealed that both C3 and C4 foods were consumed,probably from the contribution of rice(C3 plant) and millets(C4 plants) due to the coexistence of these crops at this site.In addition,the human mean δ13C value suggested that millet agriculture was only minor in human diets.The human δ15N values(6.6‰to 10.8‰,averaging 9.0‰±1.2‰,n=24) showed that animal resources played a significant role in human diets,and varied greatly.The mean δ13C value of the pigs(-14.3‰±2.5‰,n=13) was quite similar to that of the humans,but the mean δ15N value of the pigs was slightly less(1.3‰) .The similar δ13C and δ15N values between humans and pigs suggested that the pigs consumed a lot of humans'food remains.No correlations of the δ13C and δ15N values between humans and pigs showed that both human and pig diets were based mainly on plant foods,which might be related to highly developed rice-millet mixed agriculture at that time. In comparison with the human and pig diets between the two periods,millet agriculture contributed more than 10%in the Shijiahe Culture,if a simple mixing model was used.This apparent dietary shift matched the climatic variation and agricultural development through the time.In warm and humid climate with the expansion of the Qujialing Culture northwards,rice was widely cultivated.However,when the climate was cold and arid,northern culture was expanding southwards.Thus,millet agriculture became more important. | GUO Yi HU YaoWu ZHU JunYing ZHOU Mi WANG ChangSui Michael P. RICHARDS | 2011 | Science China Earth Sciences2011，54，4： | 6 |
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