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| 1 | Tumor infiltrating lymphocytes in triple negative breast cancer receiving neoadjuvant chemotherapy显示文摘AIM To determine influence of neoadjuvant-chemotherapy(NAC) over tumor-infiltrating-lymphocytes(TIL) intriple-negative-breast-cancer(TNBC).METHODS TILs were evaluated in 98 TNBC cases who came to Instituto Nacional de Enfermedades Neoplasicas from 2005 to 2010. Immunohistochemistry staining for CD3, CD4, CD8 and FOXP3 was performed in tissue microarrays(TMA) sections. Evaluation of H/E in full-face and immunohistochemistry in TMA sections was performed in pre and post-NAC samples. STATA software was used and P value < 0.05 was considered statistically significant. RESULTS Higher TIL evaluated in full-face sections from pre-NAC tumors was associated to pathologic-complete-response(pCR)(P = 0.0251) and outcome(P = 0.0334). TIL evaluated in TMA sections showed low level of agreement with full-face sections(ICC = 0.017-0.20) and was not associated to pCR or outcome. TIL in post-NAC samples were not associated to response or outcome. PostNAC lesions with pC R had similar TIL levels than those without pCR(P = 0.6331). NAC produced a TIL decrease in full-face sections(P < 0.0001). Percentage of TIL subpopulations was correlated with their absolute counts. Higher counts of CD3, CD4, CD8 and FOXP3 in pre-NAC samples had longer disease-free-survival(DFS). Higher counts of CD3 in pre-NAC samples had longer overallsurvival. Higher ratio of CD8/CD4 counts in pre-NAC was associated with pCR. Higher ratio of CD4/FOXP3 counts in pre-NAC was associated with longer DFS. Higher counts of CD4 in post-NAC samples were associated with pCR.CONCLUSION TIL in pre-NAC full-face sections in TNBC are correlated to longer survival. TIL in full-face differ from TMA sections, absolute count and percentage analysis of TIL subpopulation closely related. | Carlos A Castaneda Elizabeth Mittendorf Sandro Casavilca Yun Wu Miluska Castillo Patricia Arboleda Teresa Nunez Henry Guerra Carlos Barrionuevo Ketty Dolores-Cerna Carolina Belmar-Lopez Julio Abugattas Gabriela Calderon Miguel De La Cruz Manuel Cotrina Jorge Dunstan Henry L Gomez Tatiana Vidaurre | 2016 | World Journal of Clinical Oncology2016，7，5： | 6 |
| 2 | Clinicopathological predictors of long-term benefit in breast cancer treated with neoadjuvant chemotherapy显示文摘AIM To investigate the survival impact of clinicopathological factors, including pathological complete response(p CR) and tumor-infiltrating lymphocytes(s TIL) levels according to subtypes, in breast cancer(BC) patients who received neo-adjuvant chemotherapy(NAC).METHODS We evaluated 435 BC patients who presented and received NAC at the Instituto Nacional de Enfermedades Neoplasicas from 2003 to 2014. s TIL was analyzed as the proportion of tumor stroma occupied by lymphocytes, and was prospectively evaluated on hematoxylin and eosin-stained sections of the preN AC core biopsy. p CR was considered in the absence of infiltrating cancer cells in primary tumor and axillary lymph nodes. Analysis of statistical association between clinical pathological features, s TIL, p CR and survival were carried out using SPSSvs19.RESULTS Median age was 49 years(range 24-84 years) and the most frequent clinical stage was ⅢB(58.3%). Luminal A, Luminal B, HER2-enriched and(triple-negative) TN phenotype was found in 24.6%, 37.9%, 17.7% and 19.8%, respectively. p CR was observed in 11% and median percentage of s TIL was 40%(2%-95%) in the whole population. p CR was associated to Ct1-2(P = 0.045) and to high s TIL(P = 0.029) in the whole population. There was a slight trend towards significance for s TIL(P = 0.054) in Luminal A. s TIL was associated with grade Ⅲ(P < 0.001), no-Luminal A subtype(P < 0.001), RE-negative(P < 0.001), PgR-negative(P < 0.001), HER2-positive(P = 0.002) and p CR(P = 0.029) in the whole population. Longer disease-free survival was associated with grade Ⅰ-Ⅱ(P = 0.006), cN 0(P < 0.001), clinical stage Ⅱ(P = 0.004), ER-positive(P < 0.001), Pg R-positive(P < 0.001), luminal A(P < 0.001) and p CR(P = 0.002). Longer disease-free survival was associated with grade Ⅰ-Ⅱ in Luminal A(P < 0.001), N0-1 in Luminal A(P = 0.045) and TNBC(P = 0.01), clinical stage Ⅱ in Luminal A(P = 0.003) and TNBC(P = 0.038), and pC R in TNBC(P < 0.001). Longer overall survival was associated with grade Ⅰ-Ⅱ(P < 0.001), ER-positive(P < 0.001), PgR-positive(P < 0.001), Luminal A(P < 0.001), cN 0(P = 0.002) and p CR(P = 0.002) in the whole population. Overall survival was associated with clinical stage Ⅱ(P = 0.017) in Luminal A, older age(P = 0.042) in Luminal B, and pC R in TNBC(P = 0.005).CONCLUSION Predictive and prognostic values of clinicopathological features, like p CR and s TIL, differ depending on the evaluated molecular subtype. | Marco Galvez Carlos A Castaneda Joselyn Sanchez Miluska Castillo Lia Pamela Rebaza Gabriela Calderon Miguel De La Cruz Jose Manuel Cotrina Julio Abugattas Jorge Dunstan Henry Guerra Omar Mejia Henry L Gomez | 2018 | World Journal of Clinical Oncology2018，9，2： | 4 |
| 3 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 4 | Blood Pressure Decrease During the Acute Phase of Ischemic Stroke Is Associated With Brain Injury and Poor Stroke Outcome显示文摘 | José Castillo Rogelio Leira María M. García Joaquín Serena Miguel Blanco Antoni Dávalos | 2004 | Stroke: Journal of the American Heart Association2004，，2： | 2 |
| 5 | Clinical and histomorphometric characteristics of three different families with hereditary gingival fibromatosis显示文摘 | Jorge Kather Miguel Angel Castillo Salgado Ulisses Fernando Lodi Salgado José Roberto Cortelli Débora Pallos | 2008 | Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology2008，，3： | 1 |
| 6 | Clinical and manometric results of endorectal advancement flaps for complex anal fistula显示文摘 | Natalia Uribe Monica Millán Miguel Minguez Cristina Ballester Francisco Asencio Vicente Sanchiz Pedro Esclapez Juan Ruiz Castillo | 2007 | International Journal of Colorectal Disease2007，，3： | 1 |
| 7 | High serum levels of pro-brain natriuretic peptide (pro BNP) identify cardioembolic origin in undetermined stroke显示文摘 | Manuel Rodríguez-Yá?ez Tomás Sobrino Miguel Blanco Natalia Pérez de la Ossa David Brea Raquel Rodríguez-González Rogelio Leira Antonio Dávalos José Castillo | 2009 | Disease Markers2009，，4： | 1 |
| 8 | Semicarbazide!sensitive amine oxidase (SSAO) from human and bovine cerebrovascular tissues biochemical and immunohistological characterization显示文摘 | Vernon Castillo Jose Miguel Lizcano Joana Visa | 1998 | Neurochemistry International1998，33，： | 1 |
| 9 | Determination of low analyte concentrations by near-infrared spectroscopy: Effect of spectral pretreatments and estimation of multivariate detection limits显示文摘 | Marcel Blanco Miguel Castillo | 2007 | Analytica Chimiea Acta2007，581，： | 1 |
| 10 | Autocrine production of TGF-β confers resistance to apoptosis after an epithelial–mesenchymal transition process in hepatocytes: Role of EGF receptor ligands显示文摘 | Gaelle del Castillo Miguel M. Murillo Alberto álvarez-Barrientos Esther Bertran Margarita Fernández Aránzazu Sánchez Isabel Fabregat | 2006 | Experimental Cell Research2006，，15： | 1 |
| 11 | Asymptomatic hyperuricemia: impact of ultrasonography显示文摘 | Puig lG de Miguel E Castillo MC | 2008 | Nucleosides Nucleotides Nucleic Acids2008，27，6： | 1 |
| 12 | Plasma Cellular-Fibronectin Concentration Predicts Hemorrhagic Transformation After Thrombolytic Therapy in Acute Ischemic Stroke显示文摘 | Mar Castellanos Rogelio Leira Joaquín Serena Miguel Blanco Salvador Pedraza José Castillo Antoni Dávalos | 2004 | Stroke: Journal of the American Heart Association2004，，7： | 1 |
| 13 | Diagnostic accuracy of enthesis ultrasound in the diagnosis of early spondyloarthritis显示文摘 | de Miguel E Mu(n)oz-Fernández S Castillo C | | 0，，： | 1 |
| 14 | Diagnosis of gout in patients with asymptomatic hyperuricaemia: a pilot uhra- sound study 显示文摘 | De Miguel E Puig JG Castillo C | 2012 | Ann Rheum Dis2012，71，： | 1 |
| 15 | Asymptomatichyperuricemia:impact of ultrasonography显示文摘 | Puig JG de Miguel E Castillo MC | 2008 | NucleosidesNucleotides Nucleic Acids2008，27，： | 1 |
| 16 | Diagnosis of gout in patients with asy- mptomatic hyperuricaemia: a pilot ultrasound study 显示文摘 | De Miguel E Puig JG Castillo C | 2012 | Ann Rheum Dis2012，71，： | 1 |
| 17 | The addition of essential oils to MAP as a tool to maintain the overall quality of fruits显示文摘 | María Serrano Domingo Martínez-Romero Fabián Guillén Juan Miguel Valverde Pedro Javier Zapata Salvador Castillo Daniel Valero | 2008 | Trends in Food Science & Technology2008，，9： | 1 |
| 18 | Superiority of SDAI o- ver DAS-28 in assessment of remission in rheumatoid arthritis pa- tients using power Doppler ultrasonography as a gold standard 显示文摘 | Balsa A de Miguel E Castillo C | 2010 | Rheumatology (Oxford England)2010，49，4： | 1 |
| 19 | Asymptomatic hyperuricemia impact of ultrasonography 显示文摘 | Puig JG Miguel E Castillo MC | 2008 | Nucleosides Nucleotides Nucleic Acids2008，27，6： | 1 |
| 20 | Age Determines the Effects of Blood Pressure Lowering During the Acute Phase of Ischemic Stroke: The TICA Study显示文摘 | Rogelio Leira Mónica Millán Exuperio Díez-Tejedor Miguel Blanco Joaquín Serena Blanca Fuentes Manuel Rodríguez-Yá?ez Mar Castellanos Aida Lago Antonio Dávalos José Castillo | 2009 | Hypertension2009，，4： | 1 |
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