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| 1 | The apple DNA-binding one zinc-finger protein MdDof54 promotes drought resistance显示文摘DNA-binding one zinc-finger(Dof)proteins constitute a family of transcription factors with a highly conserved Dof domain that contains a C2C2 zinc-finger motif.Although several studies have demonstrated that Dof proteins are involved in multiple plant processes,including development and stress resistance,the functions of these proteins in drought stress resistance are largely unknown.Here,we report the identification of the MdDof54 gene from apple and document its positive roles in apple drought resistance.After long-term drought stress,compared with nontransgenic plants,MdDof54 RNAi plants had significantly shorter heights and weaker root systems;the transgenic plants also had lower shoot and root hydraulic conductivity,as well as lower photosynthesis rates.By contrast,compared with nontransgenic plants,MdDof54-overexpressing plants had higher photosynthesis rates and shoot hydraulic conductivity under long-term drought stress.Moreover,compared with nontransgenic plants,MdDof54-overexpressing plants had higher survival percentages under short-term drought stress,whereas MdDof54 RNAi plants had lower survival percentages.MdDof54 RNAi plants showed significant downregulation of 99 genes and significant upregulation of 992 genes in response to drought,and 366 of these genes were responsive to drought.We used DAPseq and ChIP-seq analyses to demonstrate that MdDof54 recognizes cis-elements that contain an AAAG motif.Taken together,our results provide new information on the functions of MdDof54 in plant drought stress resistance as well as resources for apple breeding aimed at the improvement of drought resistance. | Pengxiang Chen Mingjia Yan Lei Li Jieqiang He Shuangxi Zhou Zhongxing Li Chundong Niu Chana Bao Fang Zhi Fengwang Ma Qingmei Guan | 2020 | Horticulture Research2020，7，1： | 7 |
| 2 | Functional assembly of root-associated microbial consortia improves nutrient efficiency and yield in soybean显示文摘Root-associated microbes are critical for plant growth and nutrient acquisition. However, scant information exists on optimizing communities of beneficial root-associated microbes or the mechanisms underlying their interactions with host plants. In this report, we demonstrate that rootassociated microbes are critical influencers of host plant growth and nutrient acquisition. Three synthetic communities(SynComs) were constructed based on functional screening of 1,893 microbial strains isolated from root-associated compartments of soybean plants. Functional assemblage of SynComs promoted significant plant growth and nutrient acquisition under both N/P nutrient deficiency and sufficiency conditions.Field trials further revealed that application of SynComs stably and significantly promoted plant growth, facilitated N and P acquisition, and subsequently increased soybean yield. Among the tested communities, SynCom1 exhibited the greatest promotion effect, with yield increases of up to 36.1% observed in two field sites. Further RNA-seq implied that SynCom application systemically regulates N and P signaling networks at the transcriptional level, which leads to increased representation of important growth pathways, especially those related to auxin responses. Overall,this study details a promising strategy for constructing SynComs based on functional screening,which are capable of enhancing nutrient acquisition and crop yield through the activities of beneficial root-associated microbes. | Cunhu Wang Yanjun Li Mingjia Li Kefei Zhang Wenjing Ma Lei Zheng Hanyu Xu Baofeng Cui Ran Liu Yongqing Yang Yongjia Zhong Hong Liao | 2021 | Journal of Integrative Plant Biology2021，63，6： | 5 |
| 3 | Fabrication of gelatin methacryloyl hydrogel microneedles for transdermal delivery of metformin in diabetic rats显示文摘Injection therapy for diabetes has poor patient compliance,and the pain occurring at the site of subcutaneous injections causes significant inconvenience to diabetic patients.In this work,to demonstrate the benefits of an alternative drug delivery technique that overcomes these issues,methacrylated gelatin hydrogel-forming microneedles integrated with metformin were developed to adjust blood glucose levels in diabetic rats.Gelatin methacryloyl microneedles(GelMA-MNs)with different degrees of substitution were successfully prepared by a micro-molding method.The resultant GelMA-MNs exhibited excellent mechanical properties and moisture resistance.Metformin,an anti-diabetic drug,was further encapsulated into the GelMA-MNs,and its release rate could be controlled by the three-dimensional cross-linked network of microneedles,thereby exhibiting sustained drug release behaviors in vitro and implying a better therapeutic effect compared with that of subcutaneous injection in diabetic rats.The drug release period could be significantly prolonged by improving the cross-link density of GelMA-MNs.The results of hypoglycemic effect evaluation show that the application of GelMA-MNs for transdermal delivery in diabetic rats has promising benefits for diabetes treatment. | Zhiyong Zeng Guohua Jiang Tianqi Liu Gao Song Yanfang Sun Xueya Zhang Yanting Jing Mingjia Feng Yufei Shi | 2021 | Bio-Design and Manufacturing2021，4，4： | 4 |
| 4 | Modeling of microstructure evolution of AZ80 magnesium alloy during hot working process using a unified internal state variable method显示文摘In this paper, a unified internal state variable(ISV) model for predicting microstructure evolution during hot working process of AZ80 magnesium alloy was developed. A novel aspect of the proposed model is that the interactive effects of material hardening, recovery and dynamic recrystallization(DRX) on the characteristic deformation behavior were considered by incorporating the evolution laws of viscoplastic flow, dislocation activities, DRX nucleation and boundary migration in a coupled manner. The model parameters were calibrated based on the experimental data analysis and genetic algorithm(GA) based objective optimization. The predicted flow stress, DRX fraction and average grain size match well with experimental results. The proposed model was embedded in the finite element(FE) software DEFORM-3 D via user defined subroutine to simulate the hot compression and equal channel angular extrusion(ECAE) processes. The heterogeneous microstructure distributions at different deformation zones and the dislocation density evolution with competitive deformation mechanisms were captured.This study can provide a theoretical solution for the hot working problems of magnesium alloy. | Zexing Su Chaoyang Sun Mingjia Wang Lingyun Qian Xintong Li | 2022 | Journal of Magnesium and Alloys2022，10，1： | 3 |
| 5 | A genome-wide association study uncovers a critical role of the RsPAP2 gene in red-skinned Raphanus sativus L.显示文摘Radish(Raphanus sativus L.)taproot contains high concentrations of flavonoids,including anthocyanins(ATCs),in redskinned genotypes.However,little information on the genetic regulation of ATC biosynthesis in radish is available.A genome-wide association study of radish red skin color was conducted using whole-genome sequencing data derived from 179 radish genotypes.The R2R3-MYB transcription factor production of anthocyanin pigment 2(PAP2)gene was found in the region associated with a leading SNP located on chromosome 2.The amino acid sequence encoded by the RsPAP2 gene was different from those of the other published RsMYB genes responsible for the red skin color of radish.The overexpression of the RsPAP2 gene resulted in ATC accumulation in Arabidopsis and radish,which was accompanied by the upregulation of several ATC-related structural genes.RsPAP2 was found to bind the RsUFGT and RsTT8 promoters,as shown by a dual-luciferase reporter system and a yeast one-hybrid assay.The promoter activities of the RsANS,RsCHI,RsPAL,and RsUFGT genes could be strongly activated by coinfiltration with RsPAP2 and RsTT8.These findings showed the effectiveness of GWAS in identifying candidate genes in radish and demonstrated that RsPAP2 could(either directly or together with its cofactor RsTT8)regulate the transcript levels of ATC-related genes to promote ATC biosynthesis,facilitating the genetic enhancement of ATC contents and other related traits in radish. | Lianxue Fan YanWang Liang Xu Mingjia Tang Xiaoli Zhang Jiali Ying Cui Li Junhui Dong Liwang Liu | 2020 | Horticulture Research2020，7，1： | 2 |
| 6 | Roles of Mitogen-Activated Protein Kinases in the Modulation of Endothelial Cell Function Following Thermal Injury显示文摘 | Wei Wu Qiaobing Huang Fei He Mingjia Xiao Shiyu Pang Xiaohua Guo Ulf T. Brunk Kesen Zhao Ming Zhao | 2011 | Shock2011，，6： | 2 |
| 7 | Effects of Deformation and Phase Transformation Microstructures on Springback Behavior and Biocompatibility in β-Type Ti-15Mo Alloy显示文摘This study examined the mechanical properties, springback behavior from three-point bending loading–unloading tests and biocompatibility from human osteoblast cell adhesion and proliferation experiments in Ti-15Mo alloy with different microstructures. The springback ratio increased after the appearance of deformation microstructures including {332} < 113 > twins and dislocations, due to the increased bending strength and unchanged Young’s modulus. By contrast, the change in springback ratio was dependent on the competing effect of the simultaneous increase in bending strength and Young’s modulus after phase transformation, namely, the isothermal ω-phase formation. Good cell adhesion and proliferation were observed on the alloy surface, and they were not significantly affected by the deformation twins, dislocations and isothermal ω-phase.The diversity of deformation and phase transformation microstructures made it possible to control the springback behavior effectively while keeping the biocompatibility of the alloy as an implant rod used for spinal fixation devices. | Sujie Zhang Xiaohua Min Yada Li Weiqiang Wang Ping Li Mingjia Li | 2022 | Acta Metallurgica Sinica(English Letters)2022，35，4： | 2 |
| 8 | Novel Matching Strategy for the Coupling of Heat Flux in Furnace Side and CO_(2)Temperature in Tube Side to Control the Cooling Wall Temperatures显示文摘Required by the supercritical carbon dioxide(s CO_(2))coal-fired power cycle,s CO_(2)entering a boiler has a high temperature and can cause overheating of tubes.To eliminate the pressure drop penalty effect,the s CO_(2)boiler consists of several modules,each having different heat flux received from the furnace side(q)and different CO_(2)temperature in the cooling wall tube(T_f).We aim to search for the best matching strategy coupling furnace side and tube side to obtain the lowest temperature of tubes.By theoretically analyzing the wall temperature influenced by q,T_f and a comprehensive thermal resistance C,two matching methods are introduced:the heat flux-temperature matching(HTM)which matches higher q with lower T_f,and the heat flux-heat flux matching(HHM)that matches higher q with higher allowable-heat-flux at the temperature limit of tubes.HTM is a conventional method but HHM is newly proposed here.We show that,if C is identical for different modules,the two methods coincide;otherwise,HHM is recommended.For a s CO_(2)boiler driving 1000 MWe power plant,smaller cooling wall temperatures are obtained by HHM than HTM.Based on HHM,the mid-partition wall,heat transfer enhancement,and downward flow are comprehensively used,decreasing the wall temperature significantly. | LIU Chao MIAO Zheng XU Jinliang XU Zeyu | 2021 | Journal of Thermal Science2021，30，4： | 2 |
| 9 | A review on artificial intelligence in high-speed rail显示文摘High-speed rail(HSR)has brought a number of social and economic benefits,such as shorter trip times for journeys of between one and five hours;safety,security,comfort and on-time commuting for passengers;energy saving and environmental protection;job creation;and encouraging sustainable use of renewable energy and land.The recent development in HSR has seen the pervasive applications of artificial intelligence(AI).This paper first briefly reviews the related disciplines in HSR where AI may play an important role,such as civil engineering,mechanical engineering,electrical engineering and signalling and control.Then,an overview of current AI techniques is presented in the context of smart planning,intelligent control and intelligent maintenance of HSR systems.Finally,a framework of future HSR systems where AI is expected to play a key role is provided. | Mingjia Yin Kang Li Xiaoqing Cheng | 2020 | Transportation Safety and Environment2020，2，4： | 2 |
| 10 | Genome-wide identification of droughtresponsive microRNAs in two sets of Malus from interspecific hybrid progenies显示文摘Drought stress can negatively impact apple fruit quality and yield.Apple microRNAs(miRNAs)participate in apple tree and fruit development,as well as in biotic stress tolerance;however,it is largely unknown whether these molecules are involved in the drought response.To identify drought-responsive miRNAs in Malus,we first examined the drought stress tolerance of ten F1 progenies of R3(M.×domestica)×M.sieversii.We performed Illumina sequencing on pooled total RNA from both drought-tolerant and drought-sensitive plants.The sequencing results identified a total of 206 known miRNAs and 253 candidate novel miRNAs from drought-tolerant plants and drought-sensitive plants under control or drought conditions.We identified 67 miRNAs that were differentially expressed in drought-tolerant plants compared with drought-sensitive plants under drought conditions.Under drought stress,61 and 35 miRNAs were differentially expressed in drought-tolerant and drought-sensitive plants,respectively.We determined the expression levels of seven out of eight miRNAs by stem-loop qPCR analysis.We also predicted the target genes of all differentially expressed miRNAs and identified the expression of some genes.Gene Ontology analyses indicated that the target genes were mainly involved in stimulus response and cellular and metabolic processes.Finally,we confirmed roles of two miRNAs in apple response to mannitol.Our results reveal candidate miRNAs and their associated mRNAs that could be targeted for improving drought tolerance in Malus species,thus providing a foundation for understanding the molecular networks involved in the response of apple trees to drought stress. | Chundong Niu Haiyan Li Lijuan Jiang Mingjia Yan Cuiying Li Dali Geng Yinpeng Xie Yan Yan Xiaoxia Shen Pengxiang Chen Jun Dong Fengwang Ma Qingmei Guan | 2019 | Horticulture Research2019，6，1： | 2 |
| 11 | Highly efficient K-doped Mn-Ce catalysts with strong K-Mn-Ce interaction for toluene oxidation显示文摘In this study,K_(x)-Mn-Ce catalysts prepared by sol-gel method were investigated for toluene oxidation.Compared with Mn-Ce,the catalytic performance of K_(x)-Mn-Ce was further improved.X-ray diffraction(XRD),high resolution transmission electron microscopy(HRTEM)and Raman analyses demonstrate that K ions enter the lattice of CeO_(2) and disperse uniformly.The results of X-ray photoelectron spectroscopy(XPS),H_(2)-temperature programmed reduction(H_(2)-TPR).and O_(2)-temperature programmed desorption(O_(2)-TPD)analyses indicate that there is a strong interaction between K,Mn and Ce;the charge co mpensation effect would be induced when K ions enter the lattice of CeO_(2),which leads to more oxygen vacancies due to the generation of more Ce^(3+).Toluene-TPD shows that K-doping enhances the activation ability of toluene.Among all catalysts,K0.1-Mn-Ce shows the highest concentration of Mn^(4+),Ce^(3+),Osur,and redox ability,resulting in higher low-temperature catalytic activity.Additionally,the results of stability and water resistance also prove that K0.1-Mn-Ce catalyst possesses excellent stability and water resistance. | Bin Yang Yiqing Zeng Mingjia Zhang Fanyu Meng Shule Zhang Qin Zhong | 2023 | Journal of Rare Earths2023，41，3： | 2 |
| 12 | A reduced graphene oxide/Co3O4 composite for supercapacitor electrode显示文摘 | Xiang Chengcheng Li Ming Zhi Mingjia | 2013 | Journal of Power Source2013，226，： | 1 |
| 13 | Effect of rare emlh elements on the thermal eracking resistance of high speed steel rolls显示文摘 | Wang Mingjia Li Yanmei Wang Zixi | 2011 | Jou ''nal of rare earths2011，29，5： | 1 |
| 14 | Separation of phenothiazines in aqueous and non-aqueous capillary electrophoresis显示文摘 | Rongying Wang Xiaoning Lu Huijun Xin Mingjia Wu | 2000 | Chromatographia (-)2000，，1： | 1 |
| 15 | Parametric optimiza-tion ofregenerative Organic Rankine Cycle (ORC) for lowgrade waste heat recovery using genetic algorithm显示文摘 | Huan Xi Mingjia Li Chao Xu | 2013 | En-ergy2013，58，： | 1 |
| 16 | Purificationand characterization of a novel hypersensitive response-inducingelicitor from Magnaportheoryzae that triggers defense re-sponse in rice显示文摘 | Chen Mingjia Zeng Hongmei Qiu Dewen | 2012 | PloS ONE2012，7，5： | 1 |
| 17 | A novel elicitor identi-fied fromMagnaporthe oryzae triggers defense responses in tobaccoand rice显示文摘 | Chen Mingjia Zhang Caizhi Zi Qian | 2014 | Plant Cell Reports2014，33，11： | 1 |
| 18 | Enhanced enzymatic hydrolysis of lignocellulose by optimizing enzyme complexes显示文摘 | Mingjia Zhang Rongxin Su Wei Qi | 2010 | Applied Biochemistry and Biotechnology2010，160，： | 1 |
| 19 | An intermedi- ate-temperature solid oxide fuel cell with electrospun nanoflber cath- ode 显示文摘 | ZHI Mingjia LEE Shiwoo MILLER Nicholas | 2012 | Energy Enviro Sci2012，5，5： | 1 |
| 20 | Life Cycle Assessment Analysis and Comparison of 1000 MW S-CO_(2)Coal Fired Power Plant and 1000 MW USC Water-Steam Coal-Fired Power Plant显示文摘The objective of this paper is to understand the benefits that one can achieve for large-scale supercritical CO_(2)(S-CO_(2))coal-fired power plants.The aspects of energy environment and economy of 1000 MW S-CO_(2)coal-fired power generation system and 1000 MW ultra-supercritical(USC)water-steam Rankine cycle coal-fired power generation system are analyzed and compared at the similar main vapor parameters,by adopting the neural network genetic algorithm and life cycle assessment(LCA)methodology.Multi-objective optimization of the 1000 MW S-CO_(2)coal-fired power generation system is further carried out.The power generation efficiency,environmental impact load,and investment recovery period are adopted as the objective functions.The main vapor parameters of temperature and pressure are set as the decision variables.The results are concluded as follows.First,the total energy consumption of the S-CO_(2)coal-fired power generation system is 10.48 MJ/k Wh and the energy payback ratio is 34.37%.The performance is superior to the USC coal-fired power generation system.Second,the resource depletion index of the S-CO_(2)coal-fired power generation system is 4.38μPRchina,90,which is lower than that of the USC coal-fired power generation system,and the resource consumption is less.Third,the environmental impact load of the S-CO_(2)coal-fired power generation system is 0.742 m PEchina,90,which is less than that of the USC coal-fired power generation system,0.783 m PEchina,90.Among all environmental impact types,human toxicity potential HTP and global warming potential GWP account for the most environmental impact.Finally,the investment cost of the S-CO_(2)coal-fired power generation system is generally less than that of the USC coal-fired power generation system because the cost of the S-CO_(2)turbine is only half of the cost of the steam turbine.The optimal turbine inlet temperature T_(5)becomes smaller,and the optimal turbine inlet pressure is unchanged at 622.082°C/30 MPa. | LI Mingjia WANG Ge XU Jinliang NI Jingwei SUN Enhui | 2022 | Journal of Thermal Science2022，31，2： | 1 |
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