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| 1 | Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells显示文摘AIM: To investigate and compare the hepatogenic transdifferentiation of adipose tissue-derived stem cells (ADSC) and bone marrow-derived mesenchymal stem cells (BMSC) in vitro. Transdifferentiation of BMSC into hepatic cells in vivo has been described. Adipose tissue represents an accessible source of ADSC, with similar characteristics to BMSC.METHODS: BMSCs were obtained from patients under-going total hip arthroplasty and ADSC from human adi-pose tissue obtained from lipectomy. Cells were grown in medium containing 15% human serum. Cultures were serum deprived for 2 d before cultivating under similar pro-hepatogenic conditions to those of liver develop-ment using a 2-step protocol with sequential addition of growth factors, cytokines and hormones. Hepatic differ-entiation was RT-PCR-assessed and liver-marker genes were immunohistochemically analysed. RESULTS: BMSC and ADSC exhibited a fibroblastic mor-phology that changed to a polygonal shape when cells differentiated. Expression of stem cell marker Thy1 de-creased in differentiated ADSC and BMSC. However, the expression of the hepatic markers, albumin and CYPs increased to a similar extent in differentiated BMSC and ADSC. Hepatic gene activation could be attributed to in-creased liver-enriched transcription factors (C/EBPβ and HNF4α), as demonstrated by adenoviral expression vec-tors.CONCLUSION: Mesenchymal stem cells can be induced to hepatogenic transdifferentiation in vitro. ADSCs have a similar hepatogenic differentiation potential to BMSC, but a longer culture period and higher proliferation ca-pacity. Therefore, adipose tissue may be an ideal source of large amounts of autologous stem cells, and may become an alternative for hepatocyte regeneration, liver cell transplantation or preclinical drug testing. | Raquel Taléns-Visconti Ana Bonora Ramiro Jover Vicente Mirabet Francisco Carbonell José Vicente Castell María José Gómez-Lechón | 2006 | World Journal of Gastroenterology2006，12，36： | 35 |
| 2 | Prognostic value of increased carbohydrate antigen in patients with heart failure显示文摘AIM:To study the prognostic value of carbohydrateantigen 125(CA125) and whether it adds prognostic information to N-terminal pro-brain natriuretic peptide(NT-proBNP) in stable heart failure(HF) patients.METHODS:The predictive value of CA125 was retrospectively assessed in 156 patients with stable HF remitted to the outpatient HF unit for monitoring from 2009 to 2011.Patients were included in the study if they had a previous documented episode of HF and received HF treatment.CA125 and NT-proBNP concentrations were measured.The independent association between NT-proBNP or CA125 and mortality was assessed with Cox regression analysis,and their combined predictive ability was tested by the integrated discrimination improvement(IDI) index.RESULTS:The mean age of the 156 patients was 72 ± 12 years.During follow-up(17 ± 8 mo),27 patients died,1 received an urgent heart transplantation and 106 required hospitalization for HF.Higher CA125 values were correlated with outcomes:58 ± 85 KU/L if hospitalized vs 34 ± 61 KU/L if not(P < 0.05),and 94 ± 121 KU/L in those who died or needed urgent heart transplantation vs 45 ± 78 KU/L in survivors(P < 0.01).After adjusting for propensity scores,the highest risk was observed when both biomarkers were elevated vs not elevated(HR = 8.95,95%CI:3.11-25.73; P < 0.001) and intermediate when only NT-proBNP was elevated vs not elevated(HR = 4.15,95%CI:1.41-12.24; P < 0.01).Moreover,when CA125 was added to the clinical model with NT-proBNP,a 4%(P < 0.05) improvement in the IDI was found.CONCLUSION:CA125 > 60 KU/L identified patients in stable HF with poor survival.Circulating CA125 level adds prognostic value to NT-proBNP level in predicting HF outcomes. | Ana B Méndez Jordi Ordonez-Llanos Andreu Ferrero Mariana Noguero Teresa Mir Josefina Mora Antoni Bayes-Genis Sònia Mirabet Juan Cinca Eulàlia Roig | 2014 | World Journal of Cardiology2014，6，4： | 13 |
| 3 | Donor screening for hepatitis B virus infection in a cell and tissue bank显示文摘 | SOLVES P MIRABET V | 2008 | Transpl Infect Dis2008，10，6： | 1 |
| 4 | Twins born af- ter transplantation of ovarian conical tissue and oocyte vitrifica- tion 显示文摘 | Sanchez-Serrano M Crespo J Mirabet V | 2010 | Fertil Steril2010，93，26： | 1 |
| 5 | Twins born after transplantation of ovarian cortical tissue and oocyte vitrification 显示文摘 | Sanchez-Serrano M Crespo J Mirabet V | 2010 | Fertil Steril2010，93，1： | 1 |
| 6 | Twins born after transplantation of ovarian cortical tissue and oo- cyte vitrification显示文摘 | S6nchez-Serrano M Crespo ] Mirabet V | 2010 | Fertil Steril2010，93，1： | 1 |
| 7 | Lack of effect of glyprotein Ⅱb/Ⅲa blockade on myocardial platelet or polymorphonuclear leukocyte accumulation and on infarct size after transient coronary occlusion in pigs 显示文摘 | Barrabes JA Garcia-Dorado D Mirabet M | 2002 | J Am Coll Cardiol2002，39，1： | 1 |
| 8 | Twins born after transplantation of ovarian cortical tissue and oocyte vitrification显示文摘 | Sánchez-Serrano M Crespo J Mirabet V | | 0，，01： | 1 |
| 9 | Platelet deposition in remote cardiac regions after coronary occlusion显示文摘 | Barrabes JA Mirabet M Agullo L | 2007 | Clinical Investigation2007，37，12： | 1 |
| 10 | Volume reduction in routine cord blood banking显示文摘 | Solves P Mirabet V Roig R | | 0，，04： | 1 |
| 11 | Objective optical assessment of tear-film quality dynamics in normal and mildly symptomatic dry eyes显示文摘 | Benito A Perez GM Mirabet S | 2011 | J Cataract Refract Surg2011，37，8： | 1 |
| 12 | Objective optical assessment of tear-film quality dynamics in normal and mildly symptomatic dry eyes显示文摘 | Benito A P6rez GM Mirabet S | 2011 | J Cataract Refract Surg2011，37，8： | 1 |
| 13 | Rejection after conversion to a proliferation signal inhibitor in chronic heart transplantation显示文摘 | Francisco González‐Vílchez José A. Vázquez de Prada María J. Paniagua Luis Almenar Sonia Mirabet Manuel Gómez‐Bueno Beatriz Díaz‐Molina Jose M. Arizón Juan Delgado Félix Pérez‐Villa María G. Crespo‐Leiro Luis Martínez‐Dolz Eulalia Roig Javier Segovia José | 2013 | Clin Transplant2013，，6： | 1 |
| 14 | Banking strate- gies for improving the hematopoietic stem cell content of umbilical cord blood units for transplantation 显示文摘 | Solves P Mirabet V Perales A | 2008 | Curr Stem Cell Res Ther2008，3，2： | 1 |
| 15 | Automated separation of cord blood units in top and bottom bags using the Compomat G4显示文摘 | Solves P Mirabet V Carbonell-Uberos F | 2006 | Clin Lab haematol2006，28，3： | 1 |
| 16 | Qualitative and quantitative cell recovery in umbilical cord blood processed by two automated devices in routine cord blood banking:a comparative study显示文摘 | Solves P Planelles D Mirabet V | 2013 | Blood Transfus2013，11，： | 1 |
| 17 | Expression of A2B adenosine receptors in human lymphocytes:their role in T cell activation显示文摘 | Mirabet M Herrera C Cordero OJ | 1999 | J Cell Sci1999，112，4： | 1 |
| 18 | Banking strategies for improving the hematopoietic stem cell content of umbilical cord blood units for transplantation显示文摘 | SOLVES P MIRABET V PERALES A | 2008 | Curr Stem Cell Res Ther2008，3，2： | 1 |
| 19 | Expression of A2B adenosine receptors in human lymphocytes:their role in T cell activation显示文摘 | Mirabet M Herrera C Cordero OJ | | 0，，： | 1 |
| 20 | Use of liquid nitrogen during storage in a cell and tissue bank: Contamination risk and effect on the detectability of potential viral contaminants显示文摘 | Vicente Mirabet Manuel Alvarez Pilar Solves Dolores Ocete Concepción Gimeno | 2011 | Cryobiology2011，，2： | 1 |
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