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| 1 | Clinical relevance of Helicobacter pylori babA2 and babA2/B in Costa Rica and Japan显示文摘AIM:To evaluate the prevalence of Helicobacter pylori(H.pylori ) babA2 ,babB and a recombinant gene between babA2 and babB(babA2/B ),and their role in the development of atrophic gastritis in Costa Rican and Japanese clinical isolates.METHODS:A total of 95 continuous H.pylori-positive Costa Rican(41 males and 54 females;mean age,50.65 years;SD,± 13.04 years) and 95 continuous H.pylori-positive Japanese(50 males and 45 females;mean age,63.43;SD,± 13.21 years) patients underwent upper endoscopy from October 2005 to July 2006.They were enrolled for the polymerase chain reaction(PCR)-based genotyping of the H.pylori babA2 ,babB and babA2/B genes.Statistical analysis was performed using the χ2 test and the Fisher's exact probability test and multivariate analysis was performed by logistic regression adjusting for gender and age.P < 0.05 was regarded as statistically significant.RESULTS:The PCR-based genotyping of 95 Costa Rican and 95 Japanese isolates showed a higher prevalence of babA2 in Japan(96.8%) than in Costa Rica(73.7%),while that of babA2/B was higher in Costa Rica(11.6%) than in Japan(1.1%).In Costa Rican isolates only,babA2 was significantly associated with atrophic gastritis(P = 0.01).CONCLUSION:These results suggest that the status of babA2 and babA2/B shows geographic differences,and that babA2 has clinical relevance in Costa Rica. | Sergio A Con Hiroaki Takeuchi Mitsuaki Nishioka Norihito Morimoto Tetsuro Sugiura Nobufumi Yasuda Reinaldo Con-Wong | 2010 | World Journal of Gastroenterology2010，16，4： | 4 |
| 2 | Effect of Helicobacter pylori cdrA on interleukin-8 secretions and nuclear factor kappa B activation显示文摘AIM:To investigate genetic diversity of Helicobacter pylori (H.pylori) cell division-related gene A (cdrA) and its effect on the host response.METHODS:Inactivation of H.pylori cdrA,which is involved in cell division and morphological elongation,has a role in chronic persistent infections.Genetic property of H.pylori cdrA was evaluated using polymerase chain reaction and sequencing in 128 (77 American and 51 Japanese) clinical isolates obtained from 48 and 51 patients,respectively.Enzyme-linked immunosorbent assay was performed to measure interleukin-8 (IL-8) secretion with gastric biopsy specimens obtained from American patients colonized with cdrA-positive or-negative strains and AGS cells cocultured with wild-type HPK5 (cdrA-positive) or its derivative HPKT510 (cdrA-disruptant).Furthermore,the cytotoxin-associated gene A (cagA) status (translocation and phosphorylation) and kinetics of transcription factors [nuclear factor-kappa B (NF-κB) and inhibition kappa B] were investigated in AGS cells co-cultured with HPK5,HPKT510 and its derivative HPK5CA (cagA disruptant) by western blotting analysis with immunoprecipitation.RESULTS:Genetic diversity of the H.pylori cdrA gene demonstrated that the cdrA status segregated into two categories including four allele types,cdrA-positive (allele types;Ⅰand Ⅱ) and cdrA-negative (allele types;Ⅲ and Ⅳ) categories,respectively.Almost all Japanese isolates were cdrA-positive (Ⅰ:7.8% and Ⅱ:90.2%),whereas 16.9% of American isolates were cdrA-positive (Ⅱ) and 83.1% were cdrA-negative (Ⅲ:37.7% and Ⅳ:45.5%),indicating extended diversity of cdrA in individual American isolates.Comparison of each isolate from different regions (antrum and corpus) in the stomach of 29 Americans revealed that cdrA status was identical in both isolates from different regions in 17 cases.However,12 cases had a different cdrA allele and 6 of them exhibited a different cdrA category between two regions in the stomach.Furthermore,in 5 of the 6 cases possessing a different cdrA category,cdrA-negative isolate existed in the corpus,suggesting that cdrA-negative strain is more adaptable to colonization in the corpus.IL-8 secretions from AGS revealed that IL-8 levels induced by a cdrA-disrupted HPKT510 was significantly lower (P < 0.01) compared to wildtype HPK5:corresponding to 50%-60% of those of wild-type HPK5.These data coincided with in vivo data that an average value of IL-8 in biopsy specimens from cdrA-positive and cdrA-negative groups was 215.6 and 135.9 pg/mL,respectively.Western blotting analysis documented that HPKT510 had no effect on CagA translocation and phosphorylation,however,nuclear accumulation of NF-κB was lower by HPKT510 compared to HPK5.CONCLUSION:Colonization by a cdrA-negative or cdrA-dysfunctional strain resulted in decreased IL-8 production and repression of NF-κB,and hence,attenuate the host immunity leading to persistent infection. | Hiroaki Takeuchi Ya-Nan Zhang Dawn A Israel Richard M Peek Jr Mikio Kamioka Hideo Yanai Norihito Morimoto Tetsuro Sugiura | 2012 | World Journal of Gastroenterology2012，18，5： | 3 |
| 3 | Sodium sensitivity and cardiovascular events in patients with essential hypertension 显示文摘 | Morimoto A Uzu J Fujii T | 1997 | Lancet1997，350，9093： | 1 |
| 4 | Adverse drug events and medication errors: Detection and classification methods 显示文摘 | Morimoto T Gandhi T Seger A | 2004 | Qual SafHealth Care2004，13，4： | 1 |
| 5 | Hypertension is associated with serologically active disease in patients with systemic lupus erythematosus:role of increased Th1/Th2ratio and oxidative stress显示文摘 | Lozovoy M A Simo A N Morimoto H K | 2014 | Scand J Rheumatol2014，43，1： | 1 |
| 6 | Prenatal DNA diagnosis of a single gene disorders from maternal plasma 显示文摘 | Sekizawa A Morimoto T | 2000 | Lancet2000，356，9236： | 1 |
| 7 | Detection of GAD-reactive CD4^+ cells in so-called 'type 1B' diabetes显示文摘 | Shimada A Kodama K Morimoto J | 2003 | Ann N Y Acad Sci2003，1005，5： | 1 |
| 8 | Ventricular adrenomedullin levels correlate with the extent of cardiac hypertrophy in rats显示文摘 | Morimoto A Nishikimi T Yoshihara F | 1999 | Hypertension1999，33，5： | 1 |
| 9 | The centralrole of corticotrophin-releasing factor (CRF-41) in psycholog-ical stress in rats 显示文摘 | Morimoto A Nakamori T Morimoto K | 1993 | J Physiol1993，460，： | 1 |
| 10 | Coordinated multipoint transmission/reception techniques for LTE-advanced coordinated and distributed MIMO显示文摘 | Sawahashi M Kishiyama Y Morimoto A | 2010 | IEEE Wireless Communications2010，17，3： | 1 |
| 11 | Role of Monochamus alternatus (Coleoptera:Cerambycidae) as a vector of Bursaphelenchus xylophilus (Nematoda:Aphelenchoidae)显示文摘 | Morimoto K Iwasaki A | 1972 | Jpn For Soc1972，54，： | 1 |
| 12 | 显示文摘 | Komiya R Hayashi A Morimoto H | 2001 | Solid State Ionics2001，140，12： | 1 |
| 13 | Relationship between left ventricular geometry and natriuretie peptide levels in essential hypertension显示文摘 | Nishikimi T Yoshihara F Morimoto A | 1996 | Hypertension1996，28，1： | 1 |
| 14 | Successful treatment of congenital systemic juvenile xanthogranuloma with Langerhans cell hlstiocytosis-based chemotherapy显示文摘 | Nakatani T Morimoto A Kato R | 2004 | J Pediatr Hematol Onol2004，26，6： | 1 |
| 15 | Biological pro- duction of hydrogen from glucose by natural anaerobic mi- croflora 显示文摘 | Morimoto M Atsuko M Atif A A Y | 2004 | International Journal of Hydrogen Energy2004，29，7： | 1 |
| 16 | Structural analysis of Arabidopsis CnfU protein,an iron-sulfur cluster biosynthetic scaffold in chloroplasts显示文摘 | Yabe T Yamashita E Kikuchi A Morimoto K Nakagawa A Tsukihara T Nakai M | | 0，，： | 1 |
| 17 | Efficacy of corticosteroids in sarcoidosis presenting with atrioventricular block显示文摘 | KATO Y MORIMOTO S UEMURA A | 2003 | Sarcoidosis Vase Diffuse Lung Dis2003，20，2： | 1 |
| 18 | PHB2 protects sister-chromatid cohesion in mitosis显示文摘 | Takata H Matsunaga S Morimoto A | 2007 | Curr Biol2007，17，15： | 1 |
| 19 | Biological production of hydrogen from glucose by natural anaerobic microflora显示文摘 | Morimoto M Atsuko M Atif A A Y | 2004 | Int J Hydrogen Energy2004，29，： | 1 |
| 20 | Cardiac overexpression of monocyte chemoattractant protein-1 in transgenic mice prevents cardiac dysfunction and remodeling after myocardial infarction 显示文摘 | Morimoto H Takahashi M Izawa A | 2006 | Cire Res2006，99，8： | 1 |
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